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Gene information
General information
TC000308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13206213..13220830
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000308
    iBeetle screen iB_00066

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000308 RA
      MQFIKHTFL FVKKLTDNR FKSFYLYST VSLTANKST 
      TTNKMENTP NLVLRRVEN IIKSQEDKR LYRGLELAN 
      HMKVLLVSD PTTDKSAAA MDVNVGFMS DPRDVYGLA 
      HFCEHMLFL GTKKYPNEN DYNKYLSEH GGSSNAATY 
      PDHTIYYFD IVPDELNNA LDRFSQFFI APLFTESAT 
      DREMNAVNS EHEKNIPND VWRKDQLDK HLADPKHPY 
      HTFGTGNRH TLDTLPKEK NINVRDELL KFHDKWYSS 
      NIMCLAVLG KESLDDLEQ MVVKLFSEV KDKAIAAPR 
      WEEHPFKDE HFGTCVYMY PIKDVRNLN IVFPCRDLQ 
      EYYKSSPSH YISHLMGHE GPGSILSTL KARGWSNNL 
      VAGSRPAPR GLGFFGVTV DLTEEGIKH IDDIVELIF 
      QYLNMLKRQ GPQKWVQDE NRDIGNMLF RFKDKESPR 
      SYIAGLVHT LQDYSMEDV LSCMYLFSE WRPDIIEQV 
      WNDFVPEKI RIVVLAKQY ENELDQVEP WYGTKYKVA 
      KIPEKTLER WRKSELSGD FKLPEKNEF IPTDFELYP 
      IDKEVTEHP VIIQDTALT RVWFKQDET FLLPKANVM 
      FDFVSPLAY LDPLNCNLT HMLVQLFRD ALNEYAYAA 
      ELAGLKWEL INTKYGLIL AIGGYSNKQ HIFLDKVME 
      KLTNFKIDP KRFEICKEN YIRNLKNFA AEQPYQHAV 
      YYLAALLTE HSWTKQELL ATTEQLTID KLEAFIPQI 
      LSKMHIECL IHGNANKEK ALQLVQIVE DRLLSTLNM 
      SPLLPRQLL LNRELKLED GCNYVYEVQ NEVHKESCI 
      ELYYQCGLQ SKENNMKLE LFAQIVQEP CFDILRTKE 
      QLGYIVFSG IRRSNGVQG LRIIVQSDK HPVRLDERI 
      EEFLKNMLS YLKNMSEEE FARHREALA AQRLEKPKQ 
      LSTQTNIFW GEITSQQYH FDRANVEVA YLRTLTKED 
      IIDFYKSLL EENAQFRKK LSVHVVSMA DGGAGKMAA 
      SDKEYTVDS KGTVVCDIT QKMFVIKNT VFLLAFIAC 
      YFVNSSGGE DEDFYKLLG VPRDATVKE IRKAFKVLA 
      VKLHPDKNQ AGFNAILGE QLAYEILKD PDTRKHYDL 
      HGDTESSQK KQQYHSYTY YRDQFGIYD DDPLIVTLS 
      RADYELNIL DASQAWFIN FYSPNCHHC HELAPTWRK 
      LSSELEGVI RIGAVNCED DWSLCYQLS IESYPTLLY 
      YEKEAHLHE GQRYRGPRT LDALKEYVL SKITVSVKN 
      VDKENWERD LRKQQWLLF LCAGDNPNC PEHETRLKL 
      AAILDGLMS VGVVKDLEL CDKISNTHK SNPIVLWQV 
      DKEKNSDSE SFAILHNVQ GSDSKEILE NILGILPNP 
      ESLNEQQFK EIRAKLRNG DENPWLLCF YLGTATDLN 
      LQLKRLPSM IPSINIGLI HCGKNSALC SSLHISRYP 
      NWGILKVGG AFELHHGRD VLHELSAFA RDSSKAQNL 
      HALSPADFS NILNGHSAW FVDWYAPWC PPCRRLMPE 
      LRRASHHFA PEVVQFGTV DCTLHRNLC SQNGISSYP 
      TTILYNGSR TQVFHGTPS EDGIVEFIS DMIAPTVIT 
      LDDSSFVRL MRKPEDELW VVDFFAPWC GPCQKLAPQ 
      WRKLAKQLA EFPQIRVAQ VDCVANSDL CSAQNVRGY 
      PTIRVYPLG SKGMNTVGM YNGNRDVVS LKRWVLNLL 
      PSPVVAMDA EAFKEQILT RKFMTPWLV EFYAPWCGH 
      CTHFEPEFR KVANKLEGV IRSAKVDCE AERMFCGNL 
      RVNSYPSLF LYLSPTEKI EIDTLNVPE IISQVRNLV 
      TNRHLQHDE L
    CDS sequence
    • >TC000308 RA
      atgcagttc ataaaacac actttcctt tttgtcaag 
      aagttaact gacaatcgg ttcaaatcg ttttacttg 
      tacagtact gtaagtcta accgcaaat aagtcaacc 
      accacaaac aaaatggaa aacacacca aacttagtc 
      ttacgaaga gtcgagaat ataatcaaa tcccaagaa 
      gacaagcgg ctttatcgg ggtttggag cttgcaaat 
      cacatgaaa gttttgctt gtgagtgac cccacaacc 
      gacaaatcc gccgctgcc atggatgtc aacgttggt 
      ttcatgagc gacccacga gatgtctat ggtttggcc 
      catttctgc gaacatatg ctctttctg ggcaccaaa 
      aaatacccc aatgagaac gactacaat aaatacttg 
      agtgaacat ggggggagc agtaacgca gccacctat 
      cccgaccac acaatttac tactttgac atagtacct 
      gatgaatta aataatgct ctggatcgg ttttcgcag 
      ttttttatc gccccgttg tttaccgaa tcggccact 
      gatcgggag atgaatgct gtgaattcc gaacacgaa 
      aaaaatatc cctaatgat gtgtggaga aaggaccag 
      ttggacaaa catttggct gatccaaag cacccttac 
      cacacgttc gggacaggc aacagacat actttggat 
      acgctcccg aaggaaaaa aatattaac gtgcgagat 
      gagctgtta aagtttcac gacaagtgg tattcatca 
      aacatcatg tgtctggct gtcctagga aaagaaagt 
      cttgatgac ttggaacaa atggttgta aaattgttc 
      tctgaggtg aaagacaag gccattgcg gcccccagg 
      tgggaggag cacccgttc aaggacgag cactttggt 
      acttgtgtc tacatgtac cccatcaag gacgtccgg 
      aatttgaac atagtgttc ccctgccgt gaccttcaa 
      gagtactac aagtcgtca ccttcgcac tacatcagt 
      cacctaatg gggcacgag ggccccggc agtatttta 
      tcgacgctt aaagcccgc ggttggtcg aataattta 
      gtcgctggt agtaggcca gcgccacgc gggttgggt 
      ttttttggg gtcactgtg gatttaaca gaagagggc 
      ataaagcat attgacgat atcgtcgaa ctgattttc 
      caatatttg aacatgttg aagcgtcaa gggccccaa 
      aagtgggtg caggatgag aacagggac atcggcaat 
      atgctgttt aggtttaag gataaggaa tctccaagg 
      agttacatt gcaggccta gttcacacg ctacaagat 
      tacagtatg gaggacgtg ttaagttgt atgtatttg 
      ttttctgaa tggagaccg gatattatt gaacaagtc 
      tggaatgat tttgttccg gaaaaaatc cgaatcgta 
      gtcttggct aaacagtac gagaatgag ttggatcaa 
      gtcgagcct tggtacggt actaagtat aaagtggca 
      aagattccg gaaaaaacg ctagaaagg tggcgaaag 
      tccgaactt tctggtgat tttaagttg ccggaaaag 
      aatgagttc attccgacc gattttgag ttgtaccca 
      atcgataaa gaagttacg gaacatccg gtaattatt 
      caagacact gctctgacg agagtttgg tttaaacaa 
      gacgaaacg tttttgtta ccgaaagca aacgtcatg 
      tttgatttc gtcagtccc ttggcatat ttagatcct 
      ctcaattgt aatctgacc cacatgctg gtccaattg 
      ttccgagac gctttaaac gaatatgcc tatgccgct 
      gaacttgcc ggcttaaag tgggaactt atcaataca 
      aaatacggc ctaatcctc gccattgga ggttacagc 
      aataagcaa cacatattt ttagataag gtgatggag 
      aaattgacg aattttaaa atcgatccg aaacgattt 
      gaaatttgc aaagaaaac tacattcgt aatttgaaa 
      aactttgcg gctgagcag ccctaccag cacgctgtg 
      tattatttg gccgctctt ttgaccgag cattcctgg 
      acgaaacag gagctcttg gctacgacc gaacagctc 
      acgattgat aagttggag gcgttcatt ccccaaatc 
      ctgtcaaaa atgcacatc gagtgccta attcacggt 
      aacgcaaac aaggaaaag gcgcttcag ttggtgcaa 
      atcgtcgaa gaccgtctt ctctcaacc ctaaacatg 
      tcaccgttg cttccgcga cagttgcta ttaaatcgc 
      gagttgaaa cttgaagac ggttgtaat tacgtttac 
      gaagtccag aatgaagtc cacaaggag tcttgcatc 
      gaattgtac taccagtgc gggctccag tcgaaggag 
      aacaacatg aagttggag ttatttgct caaattgtc 
      caagagccg tgtttcgat attttacgg acgaaggaa 
      cagctgggg tatattgtt tttagcggc attcggcgg 
      tcgaatggg gtgcaaggg ttgaggatt atcgtgcag 
      tccgacaaa cacccggtg cgtttggac gaacgtatc 
      gaagagttt ttgaaaaat atgttgagt tatttgaag 
      aacatgtcg gaggaagag tttgcaaga caccgggag 
      gcgctagct gctcaaaga ctagaaaaa ccgaagcag 
      ttgtccact cagactaac attttctgg ggggagatc 
      acctcacag cagtaccac tttgacagg gccaatgtc 
      gaagtggcc tatttgcgg accctgacc aaggaggac 
      atcatcgat ttttacaag agtttgttg gaggaaaac 
      gcccagttt cgtaaaaag ctctctgtc catgtggtt 
      tcaatggcg gatgggggg gctggcaaa atggcggcc 
      agtgataag gaatacact gttgatagc aaggggacg 
      gttgtttgt gatatcacg caaaaaatg tttgttatc 
      aaaaacaca gttttttta ctcgctttt atcgcgtgc 
      tattttgta aatagttcc ggtggtgaa gatgaagat 
      ttttacaaa ttgttgggg gtgcctagg gacgccaca 
      gttaaggag attaggaag gctttcaaa gtgctagct 
      gtgaaattg catccagat aaaaatcag gctggtttt 
      aatgcaatt ttaggcgag caattggct tatgagatt 
      ctaaaagac ccagacact cgaaagcat tacgatttg 
      catggggac acagagtcc tcacaaaaa aaacaacag 
      tatcatagt tatacgtat tacagggac cagttcggc 
      atttatgat gatgacccc ctcattgtt accttaagc 
      agggccgat tatgaattg aacattttg gacgcgagt 
      caggcttgg ttcataaat ttctactct cctaactgt 
      caccactgc cacgagttg gccccaact tggcgcaaa 
      ctgtcttcc gaattagag ggggtgatc agaattggc 
      gctgttaac tgtgaagat gattggtcc ttgtgttac 
      caactcagt attgaatct taccctact ttactctat 
      tacgagaaa gaggcccac cttcacgag ggccagcgt 
      taccgcggc cctcgaact ctagacgct ttgaaagag 
      tacgttttg tcgaaaata actgttagt gtaaaaaat 
      gtagataag gaaaattgg gagcgagac ttaaggaag 
      caacaatgg ttgctgttt ttgtgtgct ggggataat 
      cctaattgt cctgaacac gagactaga ctcaaactg 
      gcggccatt ttagacggg ttaatgtca gtgggagtt 
      gtaaaagat ctggaattg tgtgataaa atttcgaat 
      actcataaa agcaaccct attgtatta tggcaagtg 
      gataaggag aagaatagt gattctgaa agttttgca 
      attttgcat aatgtgcaa ggatctgat agtaaagag 
      attctcgag aatatttta ggtatttta ccgaacccc 
      gagtcgcta aatgagcaa caatttaag gaaattagg 
      gcgaaacta cgcaacggt gacgaaaac ccgtggctg 
      ttgtgtttc tacctcggc acagccacg gatttaaac 
      ctccaactc aaacgtctt ccttccatg atcccttcc 
      ataaacata ggtttgatc cactgtggc aaaaactcg 
      gctttatgc tcaagcctc cacatctcg cgttacccc 
      aactggggc attttgaaa gtggggggc gccttcgag 
      ctgcaccac ggccgcgac gtcctccac gaactctcc 
      gcctttgcc cgcgacagc agcaaggcc caaaacctg 
      cacgcctta tcccctgcc gatttttcc aacattcta 
      aacggccac agtgcctgg tttgtggac tggtacgcc 
      ccctggtgc cccccctgc cggcgcctc atgcccgag 
      ctgcggagg gcgagccac cacttcgcc cccgaagtc 
      gtccaattc gggactgtt gactgcacg ttacacagg 
      aatttgtgc tcgcagaac gggatctcc tcctatccc 
      accacaatc ctgtacaac ggcagccgg acgcaggtg 
      ttccacggg acgccctcc gaggacggg atcgtcgag 
      tttatctca gacatgatc gcgccaact gttataacc 
      ctggatgac tcgtctttt gtccggctg atgcgcaag 
      ccggaggat gagctctgg gtggttgat tttttcgcc 
      ccgtggtgc ggcccctgc cagaagctg gccccccag 
      tggcgcaag ctcgccaaa caactggcc gagtttccg 
      caaattagg gtggctcaa gtggattgt gtcgcaaat 
      tcggatttg tgtagcgcg caaaatgtg cggggttat 
      ccgacgatt cgggtttat cccttggga agcaagggc 
      atgaatacc gttgggatg tacaacggc aatcgcgac 
      gtcgtctcg ctgaaacgc tgggttttg aacttgctg 
      ccgtcgccc gtcgtcgca atggacgct gaagcgttc 
      aaagagcaa atcctaacg aggaaattc atgacgccg 
      tggcttgtc gagttttat gcgccttgg tgcggccac 
      tgtacacat ttcgagccc gaatttcgc aaagttgca 
      aacaaatta gaaggggtt ataagaagt gccaaagtc 
      gactgcgag gccgaacga atgttttgc ggcaattta 
      cgtgtcaat agttatcct tcactattt ttgtactta 
      tcaccgacc gaaaaaatc gaaattgac acgctaaac 
      gtccccgaa attattagc caagtcagg aatttggtc 
      acaaacagg catttgcag catgacgaa ctttaa
    mRNA sequence
    • >TC000308 RA
      tgtacaatt aggtaaaac caacgtctg gatgcagtt 
      cataaaaca cactttcct ttttgtcaa gaagttaac 
      tgacaatcg gttcaaatc gttttactt gtacagtac 
      tgtaagtct aaccgcaaa taagtcaac caccacaaa 
      caaaatgga aaacacacc aaacttagt cttacgaag 
      agtcgagaa tataatcaa atcccaaga agacaagcg 
      gctttatcg gggtttgga gcttgcaaa tcacatgaa 
      agttttgct tgtgagtga ccccacaac cgacaaatc 
      cgccgctgc catggatgt caacgttgg tttcatgag 
      cgacccacg agatgtcta tggtttggc ccatttctg 
      cgaacatat gctctttct gggcaccaa aaaataccc 
      caatgagaa cgactacaa taaatactt gagtgaaca 
      tggggggag cagtaacgc agccaccta tcccgacca 
      cacaattta ctactttga catagtacc tgatgaatt 
      aaataatgc tctggatcg gttttcgca gttttttat 
      cgccccgtt gtttaccga atcggccac tgatcggga 
      gatgaatgc tgtgaattc cgaacacga aaaaaatat 
      ccctaatga tgtgtggag aaaggacca gttggacaa 
      acatttggc tgatccaaa gcaccctta ccacacgtt 
      cgggacagg caacagaca tactttgga tacgctccc 
      gaaggaaaa aaatattaa cgtgcgaga tgagctgtt 
      aaagtttca cgacaagtg gtattcatc aaacatcat 
      gtgtctggc tgtcctagg aaaagaaag tcttgatga 
      cttggaaca aatggttgt aaaattgtt ctctgaggt 
      gaaagacaa ggccattgc ggcccccag gtgggagga 
      gcacccgtt caaggacga gcactttgg tacttgtgt 
      ctacatgta ccccatcaa ggacgtccg gaatttgaa 
      catagtgtt cccctgccg tgaccttca agagtacta 
      caagtcgtc accttcgca ctacatcag tcacctaat 
      ggggcacga gggccccgg cagtatttt atcgacgct 
      taaagcccg cggttggtc gaataattt agtcgctgg 
      tagtaggcc agcgccacg cgggttggg tttttttgg 
      ggtcactgt ggatttaac agaagaggg cataaagca 
      tattgacga tatcgtcga actgatttt ccaatattt 
      gaacatgtt gaagcgtca agggcccca aaagtgggt 
      gcaggatga gaacaggga catcggcaa tatgctgtt 
      taggtttaa ggataagga atctccaag gagttacat 
      tgcaggcct agttcacac gctacaaga ttacagtat 
      ggaggacgt gttaagttg tatgtattt gttttctga 
      atggagacc ggatattat tgaacaagt ctggaatga 
      ttttgttcc ggaaaaaat ccgaatcgt agtcttggc 
      taaacagta cgagaatga gttggatca agtcgagcc 
      ttggtacgg tactaagta taaagtggc aaagattcc 
      ggaaaaaac gctagaaag gtggcgaaa gtccgaact 
      ttctggtga ttttaagtt gccggaaaa gaatgagtt 
      cattccgac cgattttga gttgtaccc aatcgataa 
      agaagttac ggaacatcc ggtaattat tcaagacac 
      tgctctgac gagagtttg gtttaaaca agacgaaac 
      gtttttgtt accgaaagc aaacgtcat gtttgattt 
      cgtcagtcc cttggcata tttagatcc tctcaattg 
      taatctgac ccacatgct ggtccaatt gttccgaga 
      cgctttaaa cgaatatgc ctatgccgc tgaacttgc 
      cggcttaaa gtgggaact tatcaatac aaaatacgg 
      cctaatcct cgccattgg aggttacag caataagca 
      acacatatt tttagataa ggtgatgga gaaattgac 
      gaattttaa aatcgatcc gaaacgatt tgaaatttg 
      caaagaaaa ctacattcg taatttgaa aaactttgc 
      ggctgagca gccctacca gcacgctgt gtattattt 
      ggccgctct tttgaccga gcattcctg gacgaaaca 
      ggagctctt ggctacgac cgaacagct cacgattga 
      taagttgga ggcgttcat tccccaaat cctgtcaaa 
      aatgcacat cgagtgcct aattcacgg taacgcaaa 
      caaggaaaa ggcgcttca gttggtgca aatcgtcga 
      agaccgtct tctctcaac cctaaacat gtcaccgtt 
      gcttccgcg acagttgct attaaatcg cgagttgaa 
      acttgaaga cggttgtaa ttacgttta cgaagtcca 
      gaatgaagt ccacaagga gtcttgcat cgaattgta 
      ctaccagtg cgggctcca gtcgaagga gaacaacat 
      gaagttgga gttatttgc tcaaattgt ccaagagcc 
      gtgtttcga tattttacg gacgaagga acagctggg 
      gtatattgt ttttagcgg cattcggcg gtcgaatgg 
      ggtgcaagg gttgaggat tatcgtgca gtccgacaa 
      acacccggt gcgtttgga cgaacgtat cgaagagtt 
      tttgaaaaa tatgttgag ttatttgaa gaacatgtc 
      ggaggaaga gtttgcaag acaccggga ggcgctagc 
      tgctcaaag actagaaaa accgaagca gttgtccac 
      tcagactaa cattttctg gggggagat cacctcaca 
      gcagtacca ctttgacag ggccaatgt cgaagtggc 
      ctatttgcg gaccctgac caaggagga catcatcga 
      tttttacaa gagtttgtt ggaggaaaa cgcccagtt 
      tcgtaaaaa gctctctgt ccatgtggt ttcaatggc 
      ggatggggg ggctggcaa aatggcggc cagtgataa 
      ggaatacac tgttgatag caaggggac ggttgtttg 
      tgatatcac gcaaaaaat gtttgttat caaaaacac 
      agttttttt actcgcttt tatcgcgtg ctattttgt 
      aaatagttc cggtggtga agatgaaga tttttacaa 
      attgttggg ggtgcctag ggacgccac agttaagga 
      gattaggaa ggctttcaa agtgctagc tgtgaaatt 
      gcatccaga taaaaatca ggctggttt taatgcaat 
      tttaggcga gcaattggc ttatgagat tctaaaaga 
      cccagacac tcgaaagca ttacgattt gcatgggga 
      cacagagtc ctcacaaaa aaaacaaca gtatcatag 
      ttatacgta ttacaggga ccagttcgg catttatga 
      tgatgaccc cctcattgt taccttaag cagggccga 
      ttatgaatt gaacatttt ggacgcgag tcaggcttg 
      gttcataaa tttctactc tcctaactg tcaccactg 
      ccacgagtt ggccccaac ttggcgcaa actgtcttc 
      cgaattaga gggggtgat cagaattgg cgctgttaa 
      ctgtgaaga tgattggtc cttgtgtta ccaactcag 
      tattgaatc ttaccctac tttactcta ttacgagaa 
      agaggccca ccttcacga gggccagcg ttaccgcgg 
      ccctcgaac tctagacgc tttgaaaga gtacgtttt 
      gtcgaaaat aactgttag tgtaaaaaa tgtagataa 
      ggaaaattg ggagcgaga cttaaggaa gcaacaatg 
      gttgctgtt tttgtgtgc tggggataa tcctaattg 
      tcctgaaca cgagactag actcaaact ggcggccat 
      tttagacgg gttaatgtc agtgggagt tgtaaaaga 
      tctggaatt gtgtgataa aatttcgaa tactcataa 
      aagcaaccc tattgtatt atggcaagt ggataagga 
      gaagaatag tgattctga aagttttgc aattttgca 
      taatgtgca aggatctga tagtaaaga gattctcga 
      gaatatttt aggtatttt accgaaccc cgagtcgct 
      aaatgagca acaatttaa ggaaattag ggcgaaact 
      acgcaacgg tgacgaaaa cccgtggct gttgtgttt 
      ctacctcgg cacagccac ggatttaaa cctccaact 
      caaacgtct tccttccat gatcccttc cataaacat 
      aggtttgat ccactgtgg caaaaactc ggctttatg 
      ctcaagcct ccacatctc gcgttaccc caactgggg 
      cattttgaa agtgggggg cgccttcga gctgcacca 
      cggccgcga cgtcctcca cgaactctc cgcctttgc 
      ccgcgacag cagcaaggc ccaaaacct gcacgcctt 
      atcccctgc cgatttttc caacattct aaacggcca 
      cagtgcctg gtttgtgga ctggtacgc cccctggtg 
      ccccccctg ccggcgcct catgcccga gctgcggag 
      ggcgagcca ccacttcgc ccccgaagt cgtccaatt 
      cgggactgt tgactgcac gttacacag gaatttgtg 
      ctcgcagaa cgggatctc ctcctatcc caccacaat 
      cctgtacaa cggcagccg gacgcaggt gttccacgg 
      gacgccctc cgaggacgg gatcgtcga gtttatctc 
      agacatgat cgcgccaac tgttataac cctggatga 
      ctcgtcttt tgtccggct gatgcgcaa gccggagga 
      tgagctctg ggtggttga ttttttcgc cccgtggtg 
      cggcccctg ccagaagct ggcccccca gtggcgcaa 
      gctcgccaa acaactggc cgagtttcc gcaaattag 
      ggtggctca agtggattg tgtcgcaaa ttcggattt 
      gtgtagcgc gcaaaatgt gcggggtta tccgacgat 
      tcgggttta tcccttggg aagcaaggg catgaatac 
      cgttgggat gtacaacgg caatcgcga cgtcgtctc 
      gctgaaacg ctgggtttt gaacttgct gccgtcgcc 
      cgtcgtcgc aatggacgc tgaagcgtt caaagagca 
      aatcctaac gaggaaatt catgacgcc gtggcttgt 
      cgagtttta tgcgccttg gtgcggcca ctgtacaca 
      tttcgagcc cgaatttcg caaagttgc aaacaaatt 
      agaaggggt tataagaag tgccaaagt cgactgcga 
      ggccgaacg aatgttttg cggcaattt acgtgtcaa 
      tagttatcc ttcactatt tttgtactt atcaccgac 
      cgaaaaaat cgaaattga cacgctaaa cgtccccga 
      aattattag ccaagtcag gaatttggt cacaaacag 
      gcatttgca gcatgacga actttaaac ggagtgtca 
      atgtcgaaa attatgcaa cactgaggc catttactt 
      tttatttgc gactttcta aagtaaaat aactgtttt 
      gctagcctg taattgaga acgaatttc gcaatttta 
      acaagattt tgttataca gggcgttta aaatttgca 
      aatttgtgg ttttaaaca ctctgtata acagccggt 
      ttctggccc aaaaagcca gatctgaac ttcgtagtt 
      ccaaagctt taaccaaat ctctgatac ttatttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY