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Gene information
General information
TC032227
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000300,TC000299
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7761455..7780806
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032227
    iBeetle screen iB_00063
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032227 RA
      MPTVGVKRN LLFEALGRT FTDDEFQRL CFEFGLELD 
      EVTTEKQMI SKEQGEDSA QAAGASEEV IYRIDIPAN 
      RYDLLCLEG ISLGIKIFQ GKIQPPVYK RIKPSSDSI 
      QKIHVTPET AKIRPYIVG AVLRNLYFT QDSYNSFID 
      LQDKLHQNI CRKRTLVSI GTHDLDTVQ GPFTYDAKP 
      PSEFKFVPL NQTKECDGN GVMELYSAH AQLKQYLHI 
      IKDSPVYPL VLDSNGVIL SLPPIINGD HSKITLNTK 
      NVLIECTAT DLTKARIVV DTLVCMFSQ YCKEPFTVE 
      ACEVITADG ESITYPDLT YRNEVISAD KANAVIGIS 
      ESPESITKL LNKMCLKSE LTSDGSSIK VTIPPTRHD 
      IIHPCDIYE DVAIAFGYN KIVRTLPKT NTIGSQNPI 
      NKLSDLLRA PIAQAGFTE ALTFSLCSR DDISTKLGL 
      KSVDEVPAV HISNPKTLE FQVCRTTLI PGILKTIAA 
      NKKMPLPLK IFEVSDVVI KDPSTEVGA RNERRICAV 
      NCNKNAGFE VVHGLLDKI MMLLEVPWN PNKTPKGYY 
      LQACEDSAF FPGRCAKVI CDGSEIGKI GVLHPNVLH 
      KFELTNPCS CVEINIEPF V
    CDS sequence
    • >TC032227 RA
      atgccgact gtgggtgtc aagcgcaat ttgctattc 
      gaagccctt gggcgcact ttcaccgac gatgaattc 
      cagcgttta tgcttcgag ttcggtctt gaactggac 
      gaagtcaca actgaaaaa cagatgata agtaaggaa 
      caaggggag gattcagcc caggcggcg ggagcctcc 
      gaggaagtc atttatcga attgacatt cctgcaaac 
      agatacgat ttattgtgt cttgaaggc atttcatta 
      ggaattaaa atatttcag gggaaaatt cagccgccg 
      gtttacaaa cgtatcaaa ccgtcatca gacagtatt 
      caaaaaatt cacgtcaca cccgaaacg gcgaaaatc 
      aggccttat atcgtaggc gccgttttg cgtaattta 
      tacttcact caagattct tacaacagt ttcattgat 
      ttgcaagat aagttgcat cagaatatt tgccgcaaa 
      cgcacgctt gtctcaatc gggacgcac gatttagat 
      acagttcag gggcccttc acctatgac gctaaacca 
      ccttctgag tttaagttt gtaccactt aatcaaacc 
      aaagagtgt gacggcaac ggcgttatg gagctctat 
      tcagcgcac gcgcagcta aaacaatac ttgcacatc 
      atcaaagac agccctgta tatccactc gttttggac 
      agtaatggg gttatttta tcccttccg ccgattatt 
      aatggggat cactccaaa attacactc aacaccaaa 
      aatgttttg attgaatgt acggcaact gatttgact 
      aaagcccgc attgttgtc gatactttg gtttgtatg 
      ttcagccag tattgcaaa gaacctttt actgtggaa 
      gcttgtgag gtgattacg gctgatggt gaatccata 
      acttacccc gacttaacg tatcgtaac gaagtgatc 
      tcagccgat aaagccaat gctgtgata ggcatcagt 
      gagtcaccg gaaagtatt acaaagctt ttaaataaa 
      atgtgtttg aagtcggaa ctgacttct gacggaagc 
      agtataaaa gtaacaatc ccacccaca agacatgac 
      attatccac ccttgcgac atctacgag gacgtggct 
      atagctttc ggttataat aaaatcgtt cgcacttta 
      cccaaaaca aacacaatc ggctcccaa aaccccatt 
      aacaaactg tcggatctt ttgcgagca ccgatcgcc 
      caagctggt ttcaccgaa gctttgacc ttttctctg 
      tgttcccga gacgacatt tcaaccaaa cttggccta 
      aaatcagtg gacgaagtc ccggctgtg cacatttca 
      aatccgaaa actctcgaa tttcaagtg tgccgcact 
      actttaatt cccgggatt ttgaaaaca atcgcagcc 
      aataaaaaa atgccgctg ccgcttaaa attttcgaa 
      gtctcggac gtcgtcatc aaggaccca tccacagaa 
      gtcggggcg agaaacgag cgccggatt tgtgcagtg 
      aattgcaac aaaaacgcc ggttttgaa gtggtccac 
      ggcctttta gacaagatt atgatgctg ttggaggtg 
      ccctggaac ccgaataag acccccaaa gggtattat 
      ctgcaagcg tgtgaagat tcggctttt tttccaggc 
      aggtgtgcc aaggtgatt tgtgacggg agcgaaatt 
      gggaaaatc ggggttttg cacccgaat gttttgcat 
      aaatttgag ctaactaat ccttgctcg tgtgtcgag 
      attaatatc gagccgttt gtgtag
    mRNA sequence
    • >TC032227 RA
      gttttttca gttgtgaaa cactttaaa cgtgttcat 
      ttttagaca aaaacgtga tagtaaata aaaaataca 
      atgccgact gtgggtgtc aagcgcaat ttgctattc 
      gaagccctt gggcgcact ttcaccgac gatgaattc 
      cagcgttta tgcttcgag ttcggtctt gaactggac 
      gaagtcaca actgaaaaa cagatgata agtaaggaa 
      caaggggag gattcagcc caggcggcg ggagcctcc 
      gaggaagtc atttatcga attgacatt cctgcaaac 
      agatacgat ttattgtgt cttgaaggc atttcatta 
      ggaattaaa atatttcag gggaaaatt cagccgccg 
      gtttacaaa cgtatcaaa ccgtcatca gacagtatt 
      caaaaaatt cacgtcaca cccgaaacg gcgaaaatc 
      aggccttat atcgtaggc gccgttttg cgtaattta 
      tacttcact caagattct tacaacagt ttcattgat 
      ttgcaagat aagttgcat cagaatatt tgccgcaaa 
      cgcacgctt gtctcaatc gggacgcac gatttagat 
      acagttcag gggcccttc acctatgac gctaaacca 
      ccttctgag tttaagttt gtaccactt aatcaaacc 
      aaagagtgt gacggcaac ggcgttatg gagctctat 
      tcagcgcac gcgcagcta aaacaatac ttgcacatc 
      atcaaagac agccctgta tatccactc gttttggac 
      agtaatggg gttatttta tcccttccg ccgattatt 
      aatggggat cactccaaa attacactc aacaccaaa 
      aatgttttg attgaatgt acggcaact gatttgact 
      aaagcccgc attgttgtc gatactttg gtttgtatg 
      ttcagccag tattgcaaa gaacctttt actgtggaa 
      gcttgtgag gtgattacg gctgatggt gaatccata 
      acttacccc gacttaacg tatcgtaac gaagtgatc 
      tcagccgat aaagccaat gctgtgata ggcatcagt 
      gagtcaccg gaaagtatt acaaagctt ttaaataaa 
      atgtgtttg aagtcggaa ctgacttct gacggaagc 
      agtataaaa gtaacaatc ccacccaca agacatgac 
      attatccac ccttgcgac atctacgag gacgtggct 
      atagctttc ggttataat aaaatcgtt cgcacttta 
      cccaaaaca aacacaatc ggctcccaa aaccccatt 
      aacaaactg tcggatctt ttgcgagca ccgatcgcc 
      caagctggt ttcaccgaa gctttgacc ttttctctg 
      tgttcccga gacgacatt tcaaccaaa cttggccta 
      aaatcagtg gacgaagtc ccggctgtg cacatttca 
      aatccgaaa actctcgaa tttcaagtg tgccgcact 
      actttaatt cccgggatt ttgaaaaca atcgcagcc 
      aataaaaaa atgccgctg ccgcttaaa attttcgaa 
      gtctcggac gtcgtcatc aaggaccca tccacagaa 
      gtcggggcg agaaacgag cgccggatt tgtgcagtg 
      aattgcaac aaaaacgcc ggttttgaa gtggtccac 
      ggcctttta gacaagatt atgatgctg ttggaggtg 
      ccctggaac ccgaataag acccccaaa gggtattat 
      ctgcaagcg tgtgaagat tcggctttt tttccaggc 
      aggtgtgcc aaggtgatt tgtgacggg agcgaaatt 
      gggaaaatc ggggttttg cacccgaat gttttgcat 
      aaatttgag ctaactaat ccttgctcg tgtgtcgag 
      attaatatc gagccgttt gtgtaggga taaggaata 
      aatggaaat tgaaattaa ctgactttt tttaccctt 
      tccaccaaa ccatgtttt aatcaaata aaacatttc 
      taacgtc