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Gene information
General information
TC000282 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13483093..13485021
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000282
    iBeetle screen iB_00058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000282 RA
      MLLLLLGTL IVILVLYLV NLSTKNFDH WEKRRVKYV 
      KPFPFVGNL LPTVLKTKS TAELIQHLY KAFPNERFV 
      GIFQFNTPI LLIRDPELI KSIAIKNFD NFVDHYGFA 
      NDDIDPLWA KNLFASQGE RWRDLRQTL SPVFTSSKM 
      RTMFVLMDE CVKQLTEYF KDQKQDVID IELKDIFSR 
      YTTDVIATT AFGIKVDSL RNRNNDFVV SGREFTDFS 
      GLRGLAFFI NGSYPRLAK FLNIKIVSD RLGNFFRTI 
      IKETLTLRE EKKIVRPDL IHSLMLARK GKLKYEELM 
      ELPEAGFAA VEESNLTKS NNNVNFLTD EDITAQALV 
      FFLGGFDTT SSLMCFAGY ELAINPHIQ KRLKDEVLA 
      TDRECNGQI TYEKLLNMK YLDMVVSET LRKWNQAVW 
      LDRKCTKEI EIESETSGE PSVTLKVGD IIWMPAYAI 
      HHDPKYYPN PELFDPERF SDENKDKIR TGTYLPFGV 
      GPRNCIGSR FALLETKLL LYNLLLNFD LVTNHKTEN 
      PIKLRRDTP LLMPQNGFQ VSLRKNDAF SEK
    CDS sequence
    • >TC000282 RA
      atgttgctt ttactatta ggcacacta atcgtgatt 
      ctagtttta tacctcgtt aatctatcg acaaagaat 
      tttgatcat tgggaaaaa cgcagagtg aaatatgtg 
      aaaccgttc ccatttgtt ggaaactta ttaccaacg 
      gtgttgaaa acaaaatca accgctgaa cttatccaa 
      cacctatac aaggctttt cctaatgaa aggttcgtg 
      ggaattttt caatttaat actcccatt ttgttgata 
      cgagaccct gaattaatc aagtctatt gcgataaaa 
      aacttcgac aatttcgtt gaccactat gggtttgct 
      aatgatgac attgatcca ttatgggcc aaaaatttg 
      tttgcaagc caaggggaa cggtggcgg gacctacgt 
      caaacttta agtcccgtg tttaccagc agcaaaatg 
      cgaacaatg ttcgtcctg atggacgaa tgcgtaaaa 
      cagttaact gaatatttc aaggaccag aagcaggat 
      gtaatagac attgaacta aaagacatt ttttcgaga 
      tacaccacc gatgtgatt gcgaccaca gccttcgga 
      atcaaggtc gattccttg agaaataga aataacgac 
      tttgtggta tcaggacga gaattcacc gatttttca 
      ggattaaga ggcctcgca tttttcatt aatggaagt 
      tatccaaga cttgccaaa tttcttaat ataaaaata 
      gtaagtgat cgcttgggt aatttcttt cgaacaata 
      atcaaagaa actctcact ttacgagaa gagaaaaaa 
      attgttcgg cctgacctc atccactcc ctaatgctg 
      gcaagaaaa ggcaaatta aaatacgag gaactaatg 
      gagttacca gaagccggt tttgccgca gtggaagaa 
      tccaacttg acaaaatct aacaacaac gtaaatttt 
      ttaaccgat gaggacata acagcccaa gctttggtc 
      ttcttcctt ggcggtttc gacacaacg tcctcactg 
      atgtgcttc gctggatac gaactcgca attaacccc 
      cacatccaa aaaagactc aaagacgaa gtcctagcc 
      accgaccgc gaatgcaac ggccaaata acgtacgaa 
      aaactccta aatatgaaa tacctggac atggtcgtg 
      tccgaaacg ttacgtaaa tggaaccaa gccgtttgg 
      ctcgaccga aaatgcacc aaagaaatt gaaatcgaa 
      tcggaaaca agtggagaa ccttcagtt actctcaaa 
      gttggagat atcatctgg atgcccgct tatgccatc 
      catcacgat cctaaatat taccctaac ccggaactt 
      ttcgatcca gagcggttt agtgacgaa aataaggac 
      aaaattaga accggcact tatctaccg ttcggagtt 
      gggcccaga aattgtatt gggtcaaga tttgcttta 
      ctggaaact aagctcttg ttgtacaac cttttgctc 
      aattttgac cttgtcacc aaccacaaa actgaaaat 
      ccgattaag ttacggaga gacacgccg ttattgatg 
      ccccaaaat gggtttcaa gtgagtctt cgaaaaaat 
      gacgctttt tccgaaaaa taa
    mRNA sequence
    • >TC000282 RA
      agaatatct tacataatc aaataatca gtttaatgg 
      aagacttga gagaaacat gttgctttt actattagg 
      cacactaat cgtgattct agttttata cctcgttaa 
      tctatcgac aaagaattt tgatcattg ggaaaaacg 
      cagagtgaa atatgtgaa accgttccc atttgttgg 
      aaacttatt accaacggt gttgaaaac aaaatcaac 
      cgctgaact tatccaaca cctatacaa ggcttttcc 
      taatgaaag gttcgtggg aatttttca atttaatac 
      tcccatttt gttgatacg agaccctga attaatcaa 
      gtctattgc gataaaaaa cttcgacaa tttcgttga 
      ccactatgg gtttgctaa tgatgacat tgatccatt 
      atgggccaa aaatttgtt tgcaagcca aggggaacg 
      gtggcggga cctacgtca aactttaag tcccgtgtt 
      taccagcag caaaatgcg aacaatgtt cgtcctgat 
      ggacgaatg cgtaaaaca gttaactga atatttcaa 
      ggaccagaa gcaggatgt aatagacat tgaactaaa 
      agacatttt ttcgagata caccaccga tgtgattgc 
      gaccacagc cttcggaat caaggtcga ttccttgag 
      aaatagaaa taacgactt tgtggtatc aggacgaga 
      attcaccga tttttcagg attaagagg cctcgcatt 
      tttcattaa tggaagtta tccaagact tgccaaatt 
      tcttaatat aaaaatagt aagtgatcg cttgggtaa 
      tttctttcg aacaataat caaagaaac tctcacttt 
      acgagaaga gaaaaaaat tgttcggcc tgacctcat 
      ccactccct aatgctggc aagaaaagg caaattaaa 
      atacgagga actaatgga gttaccaga agccggttt 
      tgccgcagt ggaagaatc caacttgac aaaatctaa 
      caacaacgt aaatttttt aaccgatga ggacataac 
      agcccaagc tttggtctt cttccttgg cggtttcga 
      cacaacgtc ctcactgat gtgcttcgc tggatacga 
      actcgcaat taaccccca catccaaaa aagactcaa 
      agacgaagt cctagccac cgaccgcga atgcaacgg 
      ccaaataac gtacgaaaa actcctaaa tatgaaata 
      cctggacat ggtcgtgtc cgaaacgtt acgtaaatg 
      gaaccaagc cgtttggct cgaccgaaa atgcaccaa 
      agaaattga aatcgaatc ggaaacaag tggagaacc 
      ttcagttac tctcaaagt tggagatat catctggat 
      gcccgctta tgccatcca tcacgatcc taaatatta 
      ccctaaccc ggaactttt cgatccaga gcggtttag 
      tgacgaaaa taaggacaa aattagaac cggcactta 
      tctaccgtt cggagttgg gcccagaaa ttgtattgg 
      gtcaagatt tgctttact ggaaactaa gctcttgtt 
      gtacaacct tttgctcaa ttttgacct tgtcaccaa 
      ccacaaaac tgaaaatcc gattaagtt acggagaga 
      cacgccgtt attgatgcc ccaaaatgg gtttcaagt 
      gagtcttcg aaaaaatga cgctttttc cgaaaaata 
      atcatttaa ttgtttgta catatgacc tgaattatt 
      taaataaat acatctgtt tgacagttt gttgctaaa 
      tttcgtcaa atatattgc ttttatcac gtttatctt 
      cgaataagt gataatgta gttgtaaac acagttatc 
      taatagctg cacatttgg caataaaaa tattcttaa 
      gcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY