Status
Please wait ...
Gene information
General information
TC000275 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 8166782..8174824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000275
    iBeetle screen iB_00057
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupation - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24 adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000275 RA
      MSDFTRSYF VDPGITIKE EPLREEPSI MNTSASVPI 
      PTRRIMEIS DYRDFGIDL DNSQSPLYP DSTLGNMQT 
      PDGFDNTTV WGNRLIISD ETIKTEALI HMDDDDIFQ 
      VDKADLIQG PTLAELNAN DDTLLGDLN FDDLLLPEE 
      NTYLNMSQK MSTAFVQNS NHLNNNSPT VTASSSCPQ 
      PSMFLYRDL DLPVASNHF DMYSAKTPV SAFSPASQS 
      SNSPQLRSS PLHMGAQKH STLHELLMK RENYISPER 
      GVAAGVQKS TPSTSPNVR MPRSSHTSR LSSSAPTHV 
      GFADIWQRR EPRKHLLST GSLAEAGST SSISTGGIL 
      SPDPQDFSH DEFDTDEES DGDHHYEDV SSEIDSDDD 
      EHVPKQSNS KKERYFWQY NVQAKGPKG QRLVMKCKM 
      EDPHVLNEV TDPVFSPDC SVRGIKHSG KARKGDGND 
      LTPNARKLF NIGKELDKL GRTINDMTP VSELPFNVR 
      PRSRKEKNK LASRACRLK KKAQHEANK IKLNGLENE 
      HRNILHGIS QCRDLVMAK LQEQSPEKQ EELSARIEN 
      IFKAATRLK VAGFTTDYV NKILEKINK GQPVTGLED 
      NI
    CDS sequence
    • >TC000275 RA
      atgagtgat tttacgagg agttatttc gtcgacccc 
      ggaataaca atcaaggag gagccctta cgtgaagaa 
      ccgtcaata atgaacact tctgccagt gtcccgatc 
      ccaacgcgc cgtataatg gaaatatcc gattaccgc 
      gacttcggc atcgacttg gacaactcg caatcgccc 
      ctgtaccca gacagcacc ttgggcaac atgcagacc 
      cctgacggc ttcgacaac acgaccgtg tggggcaat 
      cgcctgata atcagcgac gagacgata aagaccgag 
      gctttgatc cacatggac gacgacgac atcttccaa 
      gtggacaag gccgacctg atccagggc cccactttg 
      gccgaactg aacgccaac gacgacacc ctcctcggc 
      gacttgaac ttcgacgat ttgctcctc ccggaggaa 
      aacacgtac ctgaacatg tcccagaag atgtccacg 
      gcgtttgtc cagaactcc aaccacctc aacaacaac 
      agccccacg gtcacagcg tcctcctcg tgcccccag 
      cccagcatg ttcctgtac agggacctg gacctgcca 
      gttgcctcc aaccacttc gacatgtac agcgccaag 
      acccccgtt tcggccttc agccccgcc agtcagagc 
      tcgaattcg ccccagttg cggtcgtcg ccgctgcac 
      atgggggcg cagaaacac agcactttg cacgagctg 
      ctcatgaag cgggagaat tacatctcg ccggagcga 
      ggggtcgcc gctggggtg cagaaaagc acgccctcc 
      acgtcgccg aacgtgcgg atgccgagg agcagtcac 
      acctcgcgg ctgtcgtcc tcggcgccc acgcacgtg 
      ggctttgcc gatatctgg cagaggagg gagccgagg 
      aagcatttg ctgtccacg gggtcgctg gcggaggcc 
      ggatcgacg tcctccatc tccacggga gggattctg 
      agtccggat ccgcaggat ttctcgcat gatgagttt 
      gatacggat gaggagagc gatggggat catcattat 
      gaggatgtt tcgtcggaa atagactcc gatgacgac 
      gaacacgtg cctaaacaa tcaaacagc aagaaagaa 
      cggtatttc tggcaatac aacgtgcag gcaaaaggc 
      cccaaaggg cagcgtctg gtaatgaaa tgtaaaatg 
      gaagacccg cacgtcctt aacgaagta actgatccg 
      gtttttagc cctgattgt tcagttaga ggtataaaa 
      cacagcgga aaagcgcgc aaaggcgac ggcaacgat 
      ctgacgcca aacgcgcga aagcttttc aacattgga 
      aaagaacta gataagttg gggcggacg atcaacgac 
      atgacgccc gtcagcgaa ctgcccttc aacgtgagg 
      ccgaggtcc agaaaagag aagaacaaa ctggcctcc 
      agagcctgc cgtttgaag aaaaaggcc caacacgag 
      gcgaataaa atcaaactg aacgggctt gaaaatgaa 
      cacaggaac attttgcac gggatttcg caatgtagg 
      gacctggtg atggccaaa ctgcaggaa cagtcgccc 
      gaaaaacag gaagagttg tcggcaagg atcgaaaat 
      atcttcaaa gctgcaaca aggttgaaa gttgcgggt 
      tttaccaca gactacgtt aacaaaatt ctagagaaa 
      attaacaaa ggacagcct gtcacagga ttggaggat 
      aatatttaa
    mRNA sequence
    • >TC000275 RA
      tgcgtttgt tattgttgg ttttatttt tattttatt 
      tgtgttcct tgagcgatc acgtgtgtt tgtgacttt 
      gcgagtacc aaggccgcg aatatcata tccatagcc 
      aagttctgc gctgacgtc agcgaaatt aagtgtttg 
      caaataaag gaaatggct gacaatgtg gacgatcaa 
      agtgtgctg tgttgtatc aaaacagtg gttctgaca 
      gtcgtcaaa gtcgcctaa accgttcgc ttttccgat 
      tggacaatg atgatcgag ttgtcattc gttaagtga 
      taacgctgc tatcagcag atctaaaaa tacagtgtt 
      gacgttgca aatttcttg aaaatgctg tacatttaa 
      gttttgcac aaagtgaac accctcatt ggtgcaagt 
      gtaccgttc tggcttccc attggcgca gaaaattga 
      ttgaatgta ttcatgtga cctttaata aacctggtt 
      tattgtttc acatatttt gtgatttag cataaaatt 
      gttcggttt gttgtgtta tcaagtggt ttctcgtcc 
      attgtgccg tgcttgtga gtggtcgtt ggcgagtga 
      cattgcgtg agaggctat cgaagggat ccaaaacat 
      tgagtcatg ctgatggtc tgattgtta tggtggatt 
      tttcattga ctcggcacg ggatttgaa cttttatcc 
      ttggtcact agtcatgag tgattttac gaggagtta 
      tttcgtcga ccccggaat aacaatcaa ggaggagcc 
      cttacgtga agaaccgtc aataatgaa cacttctgc 
      cagtgtccc gatcccaac gcgccgtat aatggaaat 
      atccgatta ccgcgactt cggcatcga cttggacaa 
      ctcgcaatc gcccctgta cccagacag caccttggg 
      caacatgca gacccctga cggcttcga caacacgac 
      cgtgtgggg caatcgcct gataatcag cgacgagac 
      gataaagac cgaggcttt gatccacat ggacgacga 
      cgacatctt ccaagtgga caaggccga cctgatcca 
      gggccccac tttggccga actgaacgc caacgacga 
      caccctcct cggcgactt gaacttcga cgatttgct 
      cctcccgga ggaaaacac gtacctgaa catgtccca 
      gaagatgtc cacggcgtt tgtccagaa ctccaacca 
      cctcaacaa caacagccc cacggtcac agcgtcctc 
      ctcgtgccc ccagcccag catgttcct gtacaggga 
      cctggacct gccagttgc ctccaacca cttcgacat 
      gtacagcgc caagacccc cgtttcggc cttcagccc 
      cgccagtca gagctcgaa ttcgcccca gttgcggtc 
      gtcgccgct gcacatggg ggcgcagaa acacagcac 
      tttgcacga gctgctcat gaagcggga gaattacat 
      ctcgccgga gcgaggggt cgccgctgg ggtgcagaa 
      aagcacgcc ctccacgtc gccgaacgt gcggatgcc 
      gaggagcag tcacacctc gcggctgtc gtcctcggc 
      gcccacgca cgtgggctt tgccgatat ctggcagag 
      gagggagcc gaggaagca tttgctgtc cacggggtc 
      gctggcgga ggccggatc gacgtcctc catctccac 
      gggagggat tctgagtcc ggatccgca ggatttctc 
      gcatgatga gtttgatac ggatgagga gagcgatgg 
      ggatcatca ttatgagga tgtttcgtc ggaaataga 
      ctccgatga cgacgaaca cgtgcctaa acaatcaaa 
      cagcaagaa agaacggta tttctggca atacaacgt 
      gcaggcaaa aggccccaa agggcagcg tctggtaat 
      gaaatgtaa aatggaaga cccgcacgt ccttaacga 
      agtaactga tccggtttt tagccctga ttgttcagt 
      tagaggtat aaaacacag cggaaaagc gcgcaaagg 
      cgacggcaa cgatctgac gccaaacgc gcgaaagct 
      tttcaacat tggaaaaga actagataa gttggggcg 
      gacgatcaa cgacatgac gcccgtcag cgaactgcc 
      cttcaacgt gaggccgag gtccagaaa agagaagaa 
      caaactggc ctccagagc ctgccgttt gaagaaaaa 
      ggcccaaca cgaggcgaa taaaatcaa actgaacgg 
      gcttgaaaa tgaacacag gaacatttt gcacgggat 
      ttcgcaatg tagggacct ggtgatggc caaactgca 
      ggaacagtc gcccgaaaa acaggaaga gttgtcggc 
      aaggatcga aaatatctt caaagctgc aacaaggtt 
      gaaagttgc gggttttac cacagacta cgttaacaa 
      aattctaga gaaaattaa caaaggaca gcctgtcac 
      aggattgga ggataatat ttaagtgta accaaatct 
      gtaattgaa tctctatat cgcatttta ggtataagc 
      aaaaatata tttttaata accatattt atgtaattt 
      aatttgatt acaaattat cgattttta attacctgt 
      aggtacatt ttttacagc ttatgttag taggtcatg 
      atacatgat acagtgttt tgttatagt tcctattag 
      gttacgttg tttgctaca gtgcgttgt tcgtcaagt 
      cgactatgg aacttaaat tttataggt gacttgatg 
      aaccatttt taaacacac tgtattatt acgtttgaa 
      tacttattg aattgtaat agttattta tgcacctct 
      tcctgaaaa ttcgctctt aggcaggat ttcacgtct 
      ttctgtttt atttttgtc cacattggt gcataaact 
      tgttttaaa ctttcaata tgaactgca aaataaata 
      ctaaacgtt gttccaatt aacacaggt tgtgttttg 
      tcgtaattc taaattctg ttgttttta tttgagaca 
      tcacttttt gttgttttg cggggtttt catgttact 
      gatgtttta attgtttgc atgtaaatt aatggtcta 
      tattatcat aaatgttga taattatta ctatagtat 
      gttgttgca tgtagattg taaaagttt ctaaagtag 
      cgattattg tattgcgct taattgaac gaagtcggt 
      attttactt ggcaattat tgaagtaaa caagaaacc 
      aaattgaca gtgaagtga accggcagt tatttttat 
      ttctaggct ctattaggt tattagttc gtagggtag 
      attaaaggt gtttcttgg taacagttg gtgacctgc 
      tcttcaaac agttctgtg taaagagat aactatttt 
      tggaatgac cactatatt tttgtaaat tttgtaagc 
      acaccaagt gaatcgttg agacatgtg attagatga 
      taatgtgtt cagtttgtg ataagcttt ctttataat 
      aatgtaaat atagtgcaa aagtgagct caatgtaat 
      caggtattt acattaatc cataggttt ataacgtga 
      cagaattta cattgtaag ggttataaa ttaggttta 
      ttttgataa aagggctgt gttttaggg ttaattatt 
      gcgggtgag ccaagtaaa atacatgtg atgtgatga 
      cacaatggt ttgttcgag ttggtttga gcctgtgat 
      ggccataaa tatattttt acattgaac gcagacgaa 
      tatccaaag ttgatatgg ttttatact tgtagttta 
      agtattgcc atgcttatg ttttgtatt ttcatagtt 
      acagtttat gttaattta tttctatcg gttaagttt 
      gtaactctg tcgattttt gtttaatgt tcaattaat 
      aataaacga tatttataa tt