Status
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Gene information
General information
TC000271 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13719057..13723407
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000271
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000271 RA
      MSDDEDAPY VKKTKNIHY GSLEEAERA RLAAGDGND 
      DSNEKAEST SANPPQVHI STEYMELED AMSRDKQAL 
      LEEFERRRK ARSINVSTD DAEVKRNLR QLGEPICLF 
      GEGPADRRS RLREILSRI GEDAVIRRE AEEERKNFE 
      KDLETTWYH EGPESLRIA RLWIAHYSL PRAKARLEE 
      ARKAKDLST ATRTAKKQE IQKKLQIMS IYSSQIGDS 
      RPIAYCQFS PDSKLLATA GWSGLCKVW SVPDCTLKQ 
      TLKGHACNA SAIIFHPRA TISQEENIC NMASCAADG 
      SVKLWDFQS EEPIADIEG HMPHRVSRL GFHPSGRFL 
      GTCCFDCSW RLWDLQQCT EVLHQEGHV KPVYCISFQ 
      IDGSVCATG GLDSFGRVW DLRTGRCIM FMESHLKAV 
      LGIDFSPDG YHIATSSED NTCKIWDLR KRSVLYTIP 
      AHTNLISEV KFQRDGGDY LITSSYDNT AKLWTNRTW 
      QPLKTLSGH DGKIMSVDI SPDNQYIAT ASYDRTFKF 
      WAPEEPK
    CDS sequence
    • >TC000271 RA
      atgtccgac gatgaggac gcgccttac gtgaaaaaa 
      accaaaaat attcactac ggctcgttg gaggaagcg 
      gaaagagct aggttagca gccggcgat gggaacgat 
      gatagcaat gaaaaggcc gaaagcacg tcggcgaac 
      ccgccacag gttcacatt tccacagaa tacatggag 
      cttgaggac gccatgtcc cgcgacaag caagccctc 
      ttggaggaa ttcgagcgt cgtcgtaaa gcgcgctct 
      attaacgtc tccaccgat gacgccgaa gtgaaacga 
      aaccttcgc caattggga gaaccgatc tgtctattc 
      ggggaagga ccggcagat cggcgttca cgcctccgt 
      gaaatacta agtcgtatc ggtgaagac gcagtcatt 
      cgacgcgaa gccgaagaa gaacgtaaa aatttcgaa 
      aaagatctc gaaacaacg tggtatcat gaaggcccc 
      gaaagtttg cgaatagcc agattatgg atagcgcac 
      tattcactg ccgcgggcc aaagcccgg cttgaggaa 
      gccagaaaa gcgaaggat ttatcaacg gctactaga 
      acagccaag aagcaggaa atacagaaa aaactccag 
      attatgtcg atttattcg agtcagatt ggcgattcg 
      agacctatt gcctattgc cagtttagt ccagattcg 
      aaactttta gccacagct ggatggagc gggttgtgt 
      aaagtgtgg tcagtgccc gactgcaca ctcaaacag 
      accctgaaa ggtcacgcc tgtaatgcc agtgctatt 
      attttccac cctagagca acaatcagc caggaggaa 
      aatatttgc aatatggca tcgtgtgca gccgatggt 
      tcagtaaaa ttgtgggat tttcaaagc gaggaaccg 
      atcgctgat atcgagggc catatgcca catcgtgtc 
      tcccggttg ggctttcat ccctctgga aggtttctc 
      gggacttgt tgctttgac tgttcctgg agattgtgg 
      gatttgcag caatgcacc gaagtttta caccaggaa 
      ggacacgtc aaacctgtc tattgtatt tccttccag 
      attgatgga tcggtctgt gccacaggt ggtttagac 
      tcatttgga cgtgtgtgg gacttgagg accggaaga 
      tgcatcatg ttcatggag agtcactta aaggcggtt 
      ctagggata gatttttcc cctgatggc taccacatt 
      gccacgtct agcgaagac aacacttgc aaaatttgg 
      gatttgcgc aaacgttcc gtcttgtac acaatccca 
      gctcatacg aatttaatt tcggaggtg aagtttcaa 
      agggacggc ggcgattac ctaattact tcatcgtac 
      gataacacg gcgaaattg tggacaaat cggacctgg 
      caaccgttg aagaccctg tcaggtcac gacggtaaa 
      atcatgtca gttgacatc tcccccgat aatcagtac 
      atagccacg gcgtcgtac gaccgcact ttcaaattc 
      tgggccccc gaagagccc aaataa
    mRNA sequence
    • >TC000271 RA
      tggctcggt ttgacgttc agtgtccca actctccca 
      ataaaggaa ctctagggt tttattgac gttgcgagg 
      agaatcgat tgttggcag tttgcgggc caagtggag 
      tttctcatg gggtcatga aaaattgaa gggttttgc 
      tttttttaa agggggctt acgtggtcg acgttgatc 
      tctcctttg tacatgaac tcgaccaag cacttaata 
      tctacatat ttgacatct ttcatgatg aggatgggg 
      tctttttct tcgtactgg gacaggatg gtttcgaag 
      taggtgctg caggcggag aggacgatc ttgtgggcc 
      ttgatggtg cgcccctcg caggccaag gtgacgtcg 
      cagaaggcc tccttctcg aggagctgc gagaacatg 
      gtctgcaag ttcgaatgg tggtacctc catcttaga 
      caatactgt tgaggcagc atttttggt tatgttcgt 
      ccagcttcc ttccgactg ttcgcgttg gagatgacg 
      aatttacca aaaagtaat gagtcacca gcgtgagac 
      cttcgaaat aatcaatac tcgtcggtt ttggggcct 
      ttgggcgag cgtttgttt tttaaatca ccaccactt 
      cactggcac ggcacaacg catccgcaa gtcgactgt 
      ttttgcgag gtttaacaa tacaaagtc cggccgagg 
      atttctcaa aaatttgga gacgcgtat gtaaatgtt 
      cagtcattt ggaaaattc cgacaaaaa atgtgtccc 
      ttagttttt tatgagtca gtgagccaa tagaaacca 
      cgtaagacc cctataaac catgtatag tccgtatat 
      tcgccgggc caagccttt aaggcaaat caataccgc 
      acgtcttaa aaaattctc gcgtaaaaa atcttggga 
      attgtcgag gtttttcgt cacacacaa atatttgtc 
      gagagcaac ggcttttca gcctaacta acggcacca 
      aactcgact aacgcagat ccaaaacgg ggtcaaaaa 
      ttaaaccga cacagactc ggactgtac tgtaattag 
      gcctttcat atttatttt tataaactt gagattttt 
      cagagcctg ggaaagaaa gagaaaatc tatcaactg 
      ttcaagttc aacgatcgg tcgtcgcgc cgatttcgc 
      acaaattca ccgattctt tcacgattt tcgcacaca 
      aaacaccag cacggacat tgcacggct atttaggcg 
      gtttccgcg gcacaacat cgtacattt actaagatt 
      tccggaccg aacttgata atctctctc tctcatgga 
      attttttgg aaaaaaatt tagacagag atagcaaaa 
      cgctatgta aagaaaaca gaaatattt cttttgtgg 
      aagaaatcg taaaaactt gccgttggc tacactgaa 
      ctcaccagg cggttttgg ttacttttt ttgcgacaa 
      atgtcaact tgacgttac gttttgaac cgatagcag 
      tgataacat caccatgtc cgacgatga ggacgcgcc 
      ttacgtgaa aaaaaccaa aaatattca ctacggctc 
      gttggagga agcggaaag agctaggtt agcagccgg 
      cgatgggaa cgatgatag caatgaaaa ggccgaaag 
      cacgtcggc gaacccgcc acaggttca catttccac 
      agaatacat ggagcttga ggacgccat gtcccgcga 
      caagcaagc cctcttgga ggaattcga gcgtcgtcg 
      taaagcgcg ctctattaa cgtctccac cgatgacgc 
      cgaagtgaa acgaaacct tcgccaatt gggagaacc 
      gatctgtct attcgggga aggaccggc agatcggcg 
      ttcacgcct ccgtgaaat actaagtcg tatcggtga 
      agacgcagt cattcgacg cgaagccga agaagaacg 
      taaaaattt cgaaaaaga tctcgaaac aacgtggta 
      tcatgaagg ccccgaaag tttgcgaat agccagatt 
      atggatagc gcactattc actgccgcg ggccaaagc 
      ccggcttga ggaagccag aaaagcgaa ggatttatc 
      aacggctac tagaacagc caagaagca ggaaataca 
      gaaaaaact ccagattat gtcgattta ttcgagtca 
      gattggcga ttcgagacc tattgccta ttgccagtt 
      tagtccaga ttcgaaact tttagccac agctggatg 
      gagcgggtt gtgtaaagt gtggtcagt gcccgactg 
      cacactcaa acagaccct gaaaggtca cgcctgtaa 
      tgccagtgc tattatttt ccaccctag agcaacaat 
      cagccagga ggaaaatat ttgcaatat ggcatcgtg 
      tgcagccga tggttcagt aaaattgtg ggattttca 
      aagcgagga accgatcgc tgatatcga gggccatat 
      gccacatcg tgtctcccg gttgggctt tcatccctc 
      tggaaggtt tctcgggac ttgttgctt tgactgttc 
      ctggagatt gtgggattt gcagcaatg caccgaagt 
      tttacacca ggaaggaca cgtcaaacc tgtctattg 
      tatttcctt ccagattga tggatcggt ctgtgccac 
      aggtggttt agactcatt tggacgtgt gtgggactt 
      gaggaccgg aagatgcat catgttcat ggagagtca 
      cttaaaggc ggttctagg gatagattt ttcccctga 
      tggctacca cattgccac gtctagcga agacaacac 
      ttgcaaaat ttgggattt gcgcaaacg ttccgtctt 
      gtacacaat cccagctca tacgaattt aatttcgga 
      ggtgaagtt tcaaaggga cggcggcga ttacctaat 
      tacttcatc gtacgataa cacggcgaa attgtggac 
      aaatcggac ctggcaacc gttgaagac cctgtcagg 
      tcacgacgg taaaatcat gtcagttga catctcccc 
      cgataatca gtacatagc cacggcgtc gtacgaccg 
      cactttcaa attctgggc ccccgaaga gcccaaata 
      acaattttg tacaaataa ataaaacat tgttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY