Status
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Gene information
General information
TC000267 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13812266..13814077
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000267
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00054

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24 larva lethality late (L7) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000267 RA
      MPKHTRKRR HPNVNNDIE YEIDAPKNK FRAFDEDAQ 
      AEEEQLSKI LFGGASSFL KSLEEAEQE IGASCSNVD 
      SGVGEDTSD SEPQERIPA WYDEDDDGI EVGHALDAQ 
      GRKLPSGGI NDRSNKYSS LLKNKFNLI VGTPSWATL 
      DKRHKSQDS DSDEEILHS CGFLRTGTG AYLTRGALE 
      FKKVKDLNC ETYSEGPFI NAVEFHPTS RVALVGGHN 
      GVASLYAVD GRQNNKLHS VLFQHFPIF CAKFINNGN 
      EVILGSRQN HIFSYDLMA AKTTRIPLP PGMTSFKKF 
      ITFPDFDII AAAGKWGEI HLLSAASKE RVATIKQNH 
      EVTALAYGP QGNLLYGHS DCGEVTVWD VSMRRVLHK 
      FMDKGCLQG TTLAVSPTN QFLAAGSAQ GVVNLYDVG 
      DILINKVPK PIKTIMNLT TSVTDLKFN ATSEILALS 
      SAEIPNSVK LLHVGSGTV FSNFPTFET KLGHVNAIN 
      FSPNSGYVA FGNRKSTVS LYTLKHYKS Y
    CDS sequence
    • >TC000267 RA
      atgccaaaa cacacccga aaacgccgt caccccaac 
      gtaaataac gacattgag tacgaaatc gacgctccc 
      aaaaacaag tttcgagct ttcgatgaa gacgctcaa 
      gctgaagaa gagcaactc agcaaaatc ctctttggg 
      ggagcttcc agcttcctg aaaagcttg gaggaggcc 
      gagcaggaa atcggggcc tcttgttca aacgtcgac 
      tcgggggta ggtgaggac acctccgac agcgagccc 
      caggaacgc attccagct tggtatgac gaagacgac 
      gacgggatt gaagtgggg cacgctctg gatgcccaa 
      gggcgcaaa ttaccgtca gggggtatc aacgatcgc 
      agcaacaag tattcgtct ctgcttaaa aacaagttt 
      aatttgatt gtgggaacc ccctcatgg gccacgctt 
      gataaacga cacaagagc caggattcg gactccgat 
      gaggagatt ttgcacagt tgtggattt ctcaggacg 
      gggacaggc gcgtactta acgaggggc gccttggag 
      ttcaagaaa gtcaaagat ttgaattgt gagacgtac 
      agtgagggc ccctttatc aacgccgtg gagttccac 
      cccacgtca cgtgtggcc cttgtaggg gggcacaac 
      ggcgtcgcc tccctttac gccgtggat ggacgccaa 
      aacaacaag ttacacagt gttttattc caacatttc 
      ccgattttt tgtgcaaag tttatcaac aacggcaac 
      gaggtgatt ttggggtca cggcagaac cacatcttc 
      agttacgac ctaatggcg gccaaaacc accagaatc 
      ccgttaccc cctggcatg accagcttc aaaaaattc 
      atcactttc cccgatttt gacataatc gcagctgca 
      ggcaagtgg ggcgaaatc cacttgctt agcgcagcc 
      tcaaaggaa cgagtcgca acaataaag caaaaccat 
      gaagttacg gctctggcc tacggcccc caaggtaat 
      cttctctat gggcacagt gactgtggc gaagtcact 
      gtgtgggac gtgagcatg agacgggtc ttgcacaag 
      tttatggac aagggctgt ctgcaaggc accactttg 
      gcggtttcg cccactaat caatttctg gcggctggt 
      tctgcacaa ggagttgtt aatttgtac gacgtgggg 
      gatatttta attaataaa gtacctaaa cctataaag 
      accattatg aatttgact acgagcgtg accgacttg 
      aaattcaac gctacatcg gagattttg gcattgtcg 
      tctgccgaa ataccgaat tccgtcaaa ttgctccat 
      gtggggtct gggactgtt tttagcaac ttcccgacg 
      ttcgagacc aagttggga cacgtaaat gcgatcaat 
      ttctcgcca aatagtgga tatgttgcc tttgggaat 
      aggaaatcg accgtttct ttgtatacc ttgaagcac 
      tacaaaagt tattaa
    mRNA sequence
    • >TC000267 RA
      ggcgcgctg tcactttct ggttttgac agttgcttc 
      aacacctcg cttgctcta gtttccaaa attcaacaa 
      ataaccgct cgaaaatgc caaaacaca cccgaaaac 
      gccgtcacc ccaacgtaa ataacgaca ttgagtacg 
      aaatcgacg ctcccaaaa acaagtttc gagctttcg 
      atgaagacg ctcaagctg aagaagagc aactcagca 
      aaatcctct ttgggggag cttccagct tcctgaaaa 
      gcttggagg aggccgagc aggaaatcg gggcctctt 
      gttcaaacg tcgactcgg gggtaggtg aggacacct 
      ccgacagcg agccccagg aacgcattc cagcttggt 
      atgacgaag acgacgacg ggattgaag tggggcacg 
      ctctggatg cccaagggc gcaaattac cgtcagggg 
      gtatcaacg atcgcagca acaagtatt cgtctctgc 
      ttaaaaaca agtttaatt tgattgtgg gaaccccct 
      catgggcca cgcttgata aacgacaca agagccagg 
      attcggact ccgatgagg agattttgc acagttgtg 
      gatttctca ggacgggga caggcgcgt acttaacga 
      ggggcgcct tggagttca agaaagtca aagatttga 
      attgtgaga cgtacagtg agggcccct ttatcaacg 
      ccgtggagt tccacccca cgtcacgtg tggcccttg 
      taggggggc acaacggcg tcgcctccc tttacgccg 
      tggatggac gccaaaaca acaagttac acagtgttt 
      tattccaac atttcccga ttttttgtg caaagttta 
      tcaacaacg gcaacgagg tgattttgg ggtcacggc 
      agaaccaca tcttcagtt acgacctaa tggcggcca 
      aaaccacca gaatcccgt taccccctg gcatgacca 
      gcttcaaaa aattcatca ctttccccg attttgaca 
      taatcgcag ctgcaggca agtggggcg aaatccact 
      tgcttagcg cagcctcaa aggaacgag tcgcaacaa 
      taaagcaaa accatgaag ttacggctc tggcctacg 
      gcccccaag gtaatcttc tctatgggc acagtgact 
      gtggcgaag tcactgtgt gggacgtga gcatgagac 
      gggtcttgc acaagttta tggacaagg gctgtctgc 
      aaggcacca ctttggcgg tttcgccca ctaatcaat 
      ttctggcgg ctggttctg cacaaggag ttgttaatt 
      tgtacgacg tgggggata ttttaatta ataaagtac 
      ctaaaccta taaagacca ttatgaatt tgactacga 
      gcgtgaccg acttgaaat tcaacgcta catcggaga 
      ttttggcat tgtcgtctg ccgaaatac cgaattccg 
      tcaaattgc tccatgtgg ggtctggga ctgttttta 
      gcaacttcc cgacgttcg agaccaagt tgggacacg 
      taaatgcga tcaatttct cgccaaata gtggatatg 
      ttgcctttg ggaatagga aatcgaccg tttctttgt 
      ataccttga agcactaca aaagttatt aatcaaaca 
      acacatgtt agatacggt aaatgacct gttccacct 
      gttgtaaca tttatgcaa gctttataa attataaca 
      gggctattg ctaaaataa tgtgtcagt tttaatacc 
      gaagagtgg catctctcc tcaagtggt taattttga 
      tcaattttt acgttctgt atgcttttt tatttttta 
      ctaccgatg tcatgtttt agtacctgt aaataaaat 
      cgtttaagt cct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY