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Gene information
General information
TC000249 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14188599..14191471
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000249
    iBeetle screen iB_00052

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gnathal appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    gnathal appendages partially position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    gnathal appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupation - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000249 RA
      MRVFLIIAL TSLVFAQDN KEDAINSIF GTNSSFADF 
LSNYEEVTP PPLKSFGAL TKCGEGEQQ NRFVCVPYY 
NCDPNTNTV QENADIDGT NKINIRMGE DEPKCDHYL 
EICCKLAGG ANSSNLQST PPPTTTAAP NVRTDKPRG 
NSTQLANCG VRNSQGLDF NLIGSTNEA NFGEFPWML 
AILRKNPTG GQKLALCGG SLIGPRVIL TGAHCVHNV 
DVNDIKVRA GEWDTQTTN ERIPFQERN VARKIVHEH 
FLKGNLYND VALLILDRP FTKTASVGT VCLPNQDQR 
FNANDCFAT GWGKDSFGD KGRYAVILK KIQMPLVQN 
ENCQKALRG TRLGASFIL HRSFTCAGG QPNLDTCTG 
DGGSPLVCP DPSNPSRYL QVGIVAWGI GCGESQIPG 
VYADVATFR TWIDLRLQE LGINTNSYV Q
    CDS sequence
    • >TC000249 RA
      atgagagtt tttttaatc attgcttta acaagcctt 
gttttcgca caagacaat aaagaggac gctataaat 
agcatcttc ggcacgaac agttccttc gctgacttc 
ctgtccaac tacgaagaa gtgacgcct ccgccgttg 
aaaagtttc ggcgctttg acgaaatgt ggtgaaggt 
gaacaacaa aaccgtttt gtgtgtgtg ccttactac 
aactgtgac cctaacacc aacaccgtg caagaaaac 
gccgacatt gacggcacc aacaaaatc aacattcga 
atgggcgag gacgagcca aagtgtgac cattacttg 
gaaatttgc tgtaaattg gccggtggt gcgaactcc 
tccaacctc cagtcaaca cctccacca accacaaca 
gcggcccct aacgtcagg acggacaaa ccgagggga 
aatagcact caattggcc aactgcggt gttaggaac 
agccaaggg ctcgatttt aacttgatc gggagcacg 
aacgaggct aactttggc gagtttccg tggatgttg 
gctattttg aggaagaac ccaaccggt ggccagaaa 
cttgcactt tgtggcggt tcgctaatc ggcccacgg 
gtgattttg accggagcc cactgtgtt cacaatgtt 
gacgttaat gatattaaa gtcagagct ggggaatgg 
gacacgcag accaccaac gaacgaatc ccctttcag 
gagcgaaac gttgcgcgg aaaatcgtc catgagcac 
ttcctcaag ggcaatctc tacaacgac gtggcactg 
cttatccta gaccgtcct ttcaccaaa accgcaagt 
gtggggacc gtgtgtttg ccaaaccag gaccagcgg 
ttcaatgct aatgattgt ttcgccacc ggatggggg 
aaggactcc ttcggcgat aaaggcaga tacgcggtc 
attctgaaa aaaatccaa atgccgtta gtgcagaac 
gagaactgt caaaaagcg ctcaggggc acccgatta 
ggggcgtct tttatcctg cacaggtcg tttacgtgt 
gccggaggt cagccaaac ctggacact tgtacgggt 
gacgggggg agtcctttg gtgtgcccc gaccctagc 
aaccccagc cgctacttg caagtgggg atagtcgcg 
tgggggata ggttgtggt gaaagtcaa attcctggt 
gtttacgcc gatgtggcc actttccgg acttggatc 
gatttaagg ctgcaagaa ctgggcatt aacaccaac 
tcatacgtg cagtaa
    mRNA sequence
    • >TC000249 RA
      ttaggtggt tttcccccg agaaaataa gaaacattt 
cacgctccg cctgcttca aaacaatct ttacccagt 
gaagaaacg taagtcatg tgaagcggt cgcatttct 
agtcaaaaa aagtctttc agaatgaga gttttttta 
atcattgct ttaacaagc cttgttttc gcacaagac 
aataaagag gacgctata aatagcatc ttcggcacg 
aacagttcc ttcgctgac ttcctgtcc aactacgaa 
gaagtgacg cctccgccg ttgaaaagt ttcggcgct 
ttgacgaaa tgtggtgaa ggtgaacaa caaaaccgt 
tttgtgtgt gtgccttac tacaactgt gaccctaac 
accaacacc gtgcaagaa aacgccgac attgacggc 
accaacaaa atcaacatt cgaatgggc gaggacgag 
ccaaagtgt gaccattac ttggaaatt tgctgtaaa 
ttggccggt ggtgcgaac tcctccaac ctccagtca 
acacctcca ccaaccaca acagcggcc cctaacgtc 
aggacggac aaaccgagg ggaaatagc actcaattg 
gccaactgc ggtgttagg aacagccaa gggctcgat 
tttaacttg atcgggagc acgaacgag gctaacttt 
ggcgagttt ccgtggatg ttggctatt ttgaggaag 
aacccaacc ggtggccag aaacttgca ctttgtggc 
ggttcgcta atcggccca cgggtgatt ttgaccgga 
gcccactgt gttcacaat gttgacgtt aatgatatt 
aaagtcaga gctggggaa tgggacacg cagaccacc 
aacgaacga atccccttt caggagcga aacgttgcg 
cggaaaatc gtccatgag cacttcctc aagggcaat 
ctctacaac gacgtggca ctgcttatc ctagaccgt 
cctttcacc aaaaccgca agtgtgggg accgtgtgt 
ttgccaaac caggaccag cggttcaat gctaatgat 
tgtttcgcc accggatgg gggaaggac tccttcggc 
gataaaggc agatacgcg gtcattctg aaaaaaatc 
caaatgccg ttagtgcag aacgagaac tgtcaaaaa 
gcgctcagg ggcacccga ttaggggcg tcttttatc 
ctgcacagg tcgtttacg tgtgccgga ggtcagcca 
aacctggac acttgtacg ggtgacggg gggagtcct 
ttggtgtgc cccgaccct agcaacccc agccgctac 
ttgcaagtg gggatagtc gcgtggggg ataggttgt 
ggtgaaagt caaattcct ggtgtttac gccgatgtg 
gccactttc cggacttgg atcgattta aggctgcaa 
gaactgggc attaacacc aactcatac gtgcagtaa 
ttacgttat tgtaaattt atcgtggct taataaatc 
tgaaataat atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY