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Gene information
General information
TC000238 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid9: 326149..337176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000238
    iBeetle screen iB_00050
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000238 RA
      MHFVKDDVV ISGIGGYFP KALNIEKLQ EHLLNNENL 
      LESRWKAGE RGVSNKIGV VPTEYFDNA YFGIHRQQC 
      TFMDPMHRL ILERTYEAL IDAGVNPSE VRGKRIGCF 
      MASSLGEND NLFLESVVS GFGVTGHSR AMMPNRVSY 
      WLDLKGPSV AYDSNWIGG IEVVRLAYE AIKTGQCEA 
      VLVGTANLA LNSEFQWLY NDMGLLSAD GSTRAYDID 
      AQGYARSDG VVVLYIQRA SDAKRSYAS IVNVATQFD 
      GNREGTLLD IDSNNMAEF ISDFYKDID VDPKDVEFV 
      ETYGSALKE TDRKELEAL EKVYCKNRQ NPLLIGTVK 
      PNTGHSEAS AALFSIVKV CIAMEAETI PATIQYQTP 
      NPELKPLLN GSMEVVTQN RKWGVKYAA VNAIGLDSY 
      YGHLLLKAN PKKKVNAQV QDDIPRLIL ASTRTEAGI 
      KEILQTIKS RATIDPEYV KLIQDLYSK PILGHLYRG 
      YTILGAEAP KEETEYHLG NKRQIWFVY SGMGSQWSG 
      MASDLMKLP VFANAIHKC HKVLVDKGI NLLEIITSK 
      DKTMFDNIL HSFVGIAAI QIALTDVLR AVGIVPDGI 
      IGHSVGELG CAYADNCMT AEQMILSAY SRGRASLEA 
      TLIRGMMAA IGMGYQQIK DKCPPSIEV ACHNGPDSS 
      TISGPTEDM EKFVKELQD QGIFARLVN VSNIAYHSR 
      YIKPAAPGL LKYLKEVLP EPVARSSKW ISTSNPEEN 
      WETELAKHS SAEYHTNNL LSSVLFEEG LKHIPKDSV 
      LIEIAPHGL LQAILKRSV KSGCTNVSL TQRGSNSGV 
      EFLLSAFGK LYLAGLDMD ISKLFPPVE YPVSRGTPC 
      LANLSHWEH SEVWRTGLE EKLKSLFGV RDYQVTLNS 
      EEFRECVGH QLDDKIILP CTSYLSTIF DIVANISTG 
      HKEFIFENL HFKKMLTVP RIGSVALHA MIQKGSGDF 
      EIIAGNDIL VTGTLTFPN PADKFMIDP IKVEVSDDN 
      VRLSGTDVY NEFQHRGHK YSGQYKSIK SLVVCENGS 
      VSLAQWNNK WTMFVEAMI QQHLFQDGE RSQQIYVPK 
      NIHKIVVNK ELLPNDKTD LEVHYDFAT GLITCDGIQ 
      VVGLKAVPF PKDPKAISF DSIEFTPFN LSNLSIENG 
      INLALQLTL DNFSEQFVT TISINEIEG KNPPLLENI 
      QNVCSMYSK LNSTVTSVK DPQTIDLQV SYPFFVVLN 
      DTINDEVAK LTSSSHGFL LVRSSKQIL AYPQIAQVA 
      QFSVNGVEY SILRRAKTT QPLIVTVKG DTLSIKDLT 
      RTSASWVNE LDTAAEAAK AKKNSVYLV SSLVPTEGM 
      RNFVEELLS TPKMSNVRV LFALDRRIG NVNVKDPQL 
      QEIFKLDLV LTVIKDGTL GSVLPVPLK LKTNIQNNF 
      GITSNAIKD KVINYVGIN LKDETILPV SEKKKELGN 
      IDYSGVGTN GQKVMGLAQ LDTDLCQLV PDSILSWNV 
      PDKWSLEEA ATIPHAYVS AYYCLFEKA WLKSGEIVL 
      VHAGCTAIG LAAISIAAS YDCKIYTTV ATEWQRSFL 
      KKQFDCLKD ENIFSLEDS SFEPLILMA TGGVGAHVI 
      INCLSGHLL QSSVGCLAE YGRLIQIGQ HDCEENNTI 
      GMSVFLKNT SFFVVKLEN IFLESNEVK QEIRDMVQK 
      GIESKTVRP INRKVVEHY NIVEILNSL SQSGNVGKI 
      LIKINNNLL MNKFILNNP SQFICDSKS SYLIYGGSA 
      EQWTDAVEW LVFRGARKI VVSSDSKPQ QVYVNRRLS 
      LLQSCYSAD IIYAPPKAH TKEGATELL TEVYHLGPI 
      HCVFSLPVK SNDSRISKI KPIQYIDNA LRTIAPKAI 
      LINFVNHAA GICQFRTEA NFPSYHIQW HKDLDFGEI 
      IYGLDEILT YNVRDILIK NDRVSDSHQ ETTQSLFKK 
      LTLILPNSL EDFIEETKS APAKPQLIQ LRTLGPREI 
      RELAPVFII PGLSGFKEM NEMAFQLLY PTFCAVLPS 
      TPMPIKELA LDLVEQIKK VWPKGAYNI IGVSWGGAL 
      MVEIAKILD KQGASLHLY FIDAAPTTL QAAVKHLGE 
      SATSIETNL LTRVLKIND AEVVRKIEE APGWDSRIK 
      LALDHFEGH LSDKKALGQ GLFTLKSHL QNLLTFSPS 
      EELVSGQIH LIRPTGTSK YDNCGLVSY CKQTPQITL 
      VNGDHLSII NSPVTTEYI NEKHYLI
    CDS sequence
    • >TC000238 RA
      atgcatttc gtaaaagac gacgttgtc atttccggc 
      attggcggt tacttcccc aaagctttg aacatcgaa 
      aaattgcaa gaacacctc ctcaacaat gagaatctc 
      ctagagagc cgctggaag gccggcgag cgcggcgtt 
      tccaataaa atcggtgtc gtcccaact gaatatttc 
      gacaatgct tatttcgga attcatcgt cagcagtgc 
      actttcatg gaccccatg caccgactc atccttgaa 
      cgcacctac gaagcgctc attgacgcc ggtgttaac 
      ccgagcgag gttcgcggt aaaagaatc ggttgtttc 
      atggcttcg tctctcgga gagaacgat aatttgttt 
      ctggagtct gtggtgagc gggtttggt gtcactgga 
      cactctagg gctatgatg ccaaacagg gtgtcctac 
      tggctggac cttaaaggg ccaagtgtt gcgtatgat 
      agtaactgg attggaggt atagaagtg gtaaggttg 
      gcctatgaa gctatcaaa acaggtcaa tgtgaggcg 
      gttttggtc ggaaccgct aatttggcc cttaattcg 
      gagttccaa tggttgtat aatgacatg ggtctgtta 
      tcggctgat ggtagtact agggcgtac gatattgat 
      gcccagggt tatgcacga agcgatggg gttgtcgtt 
      ttgtacatc caacgtgcc agtgacgct aaacgttcc 
      tacgccagc attgtcaat gttgccact caatttgac 
      ggcaaccgg gaaggaact ctcttagat atagactct 
      aataacatg gcagaattc attagcgac ttttacaag 
      gacattgac gttgatccg aaagacgtt gagtttgtt 
      gaaacgtac ggaagcgcc cttaaagaa accgatcgt 
      aaagaacta gaagcgtta gaaaaagtc tattgcaaa 
      aaccgccaa aaccctctc cttatcggc accgtcaag 
      cccaacacc ggtcacagc gaagcttct gccgcgctt 
      ttcagcatt gtcaaagtt tgtatagcc atggaggcc 
      gagacaatt ccggcaaca atccagtac caaacgcca 
      aatcccgaa ttaaaaccg ctccttaac gggagtatg 
      gaagtggtg acacagaat cgtaaatgg ggggttaaa 
      tatgccgca gttaatgcg attggtttg gattcgtac 
      tatggacac ctccttttg aaagccaat cccaaaaag 
      aaggtcaat gctcaagtt caggacgat attccaaga 
      ctcatactt gcgtccact cgaactgag gctgggatt 
      aaggaaatt ttgcaaact atcaagtcg cgcgcaacg 
      attgatcct gaatatgtt aaactcatc caggatttg 
      tattctaaa ccgattttg gggcacttg taccgcggt 
      tatacgatt ttgggggct gaggccccg aaagaggaa 
      actgagtac catttgggc aacaagcgc caaatttgg 
      ttcgtctac tctgggatg ggcagccaa tggagcggc 
      atggccagt gacttgatg aaactccca gtttttgca 
      aacgcaatc cacaaatgc cacaaagta ttagtcgac 
      aaaggcatc aacttgctt gaaatcatc accagtaaa 
      gacaagacc atgttcgac aacattttg cactcattt 
      gtgggaatc gccgcaatt caaattgcc ttaaccgac 
      gttttgcgg gctgttggt atcgtccca gatggtata 
      atcgggcat tccgtgggc gagctaggg tgtgcatac 
      gccgataat tgcatgacg gccgaacaa atgatttta 
      tcggcctac tcccgcggg agagcctca cttgaagcc 
      accctaatc cgcggcatg atggctgcc atcggcatg 
      gggtaccaa caaatcaaa gacaagtgc cccccgtcc 
      atagaagtc gcgtgtcac aatggcccc gacagctcc 
      acaatctcg ggccccacc gaagacatg gaaaagttc 
      gtcaaggag ttgcaagac caggggatt ttcgcccgt 
      cttgtcaat gtttccaac attgcctac cacagcagg 
      tacatcaaa cccgctgct ccaggcctc ttaaaatac 
      ctcaaggag gtgctcccc gaacccgtg gcccgctcc 
      tcaaagtgg atcagtacc tccaacccg gaggaaaac 
      tgggagacg gaactggcc aaacactcc tcggccgaa 
      tatcacacc aacaatctc ctgagctcg gttttgttc 
      gaagagggc ttgaaacac atccctaag gactcggtt 
      ttgatcgaa atcgcccca catgggctg ctccaagcc 
      attttgaaa cgctcggtc aagtcaggt tgtaccaac 
      gtttccctg actcagaga ggctcaaac agcggggtt 
      gaattccta ctttcggcg ttcggtaaa ttgtacttg 
      gctggttta gatatggac atttctaag ctgttcccg 
      ccggttgag tacccagta agtcgggga accccctgt 
      ttggcgaac ttatcgcat tgggagcac agcgaagtt 
      tggagaact gggcttgag gagaaactg aaatcgctg 
      ttcggggtg cgtgactac caagtcacg ctgaacagc 
      gaggagttc cgagagtgt gtggggcac caacttgac 
      gacaaaatc atactaccg tgcacctca tacttgagc 
      accatcttc gacattgtc gcaaacata agcactggg 
      cacaaggaa ttcatcttt gagaatctc cactttaag 
      aaaatgtta accgtcccg agaatcggt tcggtggcg 
      cttcacgca atgatccag aagggaagc ggcgatttt 
      gagataatc gccgggaac gacattctc gtaactgga 
      actttgaca ttcccgaac ccggccgat aaattcatg 
      attgatcca atcaaagtg gaggtgtct gacgacaac 
      gtccgctta tccgggaca gacgtttac aacgaattc 
      caacaccgg ggacacaaa tacagcggt cagtacaaa 
      tccatcaag agtctagtc gtgtgcgaa aacggctca 
      gtcagcctg gcacagtgg aacaacaaa tggaccatg 
      tttgtggaa gccatgatc caacaacac ttgttccaa 
      gacggcgaa cgctcgcag caaatctac gtcccgaaa 
      aacattcac aaaatcgtc gtgaacaaa gagcttttg 
      cctaatgat aaaaccgac ctggaggtg cattacgat 
      tttgcgacc gggctgatc acctgtgac gggatccag 
      gtggttggg cttaaggct gtgcccttc cccaaggac 
      ccgaaggca atttcgttt gattcaatt gagtttact 
      ccctttaat ttgtccaac ttgagtatt gagaacgga 
      attaatctc gctcttcaa ctcactttg gacaacttt 
      tccgagcaa tttgttacc acaatcagc atcaatgaa 
      atcgagggc aagaacccg cctcttttg gaaaacatc 
      cagaacgtg tgctccatg tactcgaaa cttaacagc 
      accgtaacg tcagtcaaa gacccccaa actatagac 
      ttacaagtc tcctacccc tttttcgtc gttttaaac 
      gacacaatt aacgacgaa gtcgcaaaa ttaacatca 
      tcatcacac gggtttttg ttagtgcgt tcaagcaag 
      caaatcctg gcgtaccct cagatcgca caagtggcg 
      caatttagt gttaacgga gttgaatac tcaattttg 
      cgccgtgct aaaaccacc caaccgctc attgtcaca 
      gtgaaaggc gacacttta agcatcaaa gacttgaca 
      agaacttca gcttcatgg gttaatgaa ttagatacg 
      gctgcggaa gcagccaaa gccaagaaa aatagtgtt 
      tatctggtc tcgtcttta gtcccgacc gaaggcatg 
      cgcaacttt gttgaagag ttgctctca acacctaaa 
      atgtccaac gttcgggtg cttttcgcc ctggacaga 
      cgtatcgga aacgttaat gttaaagac ccccagttg 
      caagaaatc ttcaaactt gacttggtt ttgacggtt 
      attaaggac gggactttg ggctcggtc ttgccagtg 
      ccactgaaa ctcaaaacc aacatccag aacaatttc 
      ggcatcact agcaatgcg atcaaggat aaagtcatc 
      aattatgtc ggtattaat ttgaaagac gagactatt 
      ttgccagtc agtgagaag aaaaaagag ttgggtaat 
      attgattat tccggggtt ggaacaaac gggcaaaaa 
      gtgatgggt cttgcccag cttgacaca gacttgtgc 
      caactggtc cctgactca attttgtcc tggaacgtt 
      cctgataaa tggagtttg gaggaagct gcaaccatc 
      ccgcacgcc tacgttagc gcttattac tgcctgttt 
      gagaaagcc tggctgaaa tcaggcgag atcgtccta 
      gttcatgcc ggttgtacg gccatcggc ttagctgca 
      atttcgata gctgccagt tatgattgt aaaatttac 
      accaccgta gccaccgaa tggcaaaga agtttcctc 
      aaaaaacaa ttcgattgc ttgaaagac gagaatatt 
      ttcagtttg gaggattca agcttcgag cctttgatt 
      ctaatggca actggtggt gttggggct catgttatt 
      attaattgc ctctctgga catctcctc caatcgtca 
      gtcggttgt ctggccgag tacgggaga ctgattcag 
      attggtcag cacgattgt gaggaaaat aacacaatc 
      gggatgagc gtttttttg aaaaatacc tcgttcttt 
      gtggtcaaa cttgagaat attttcttg gaatcgaac 
      gaggttaag caggagatt agggatatg gttcagaag 
      ggcattgag agtaaaact gtgaggcca attaatcga 
      aaagtcgtg gagcattac aacattgtg gagatttta 
      aattcgcta agtcagtcc ggaaacgtg ggcaaaatc 
      ttgatcaaa atcaacaac aacttgcta atgaacaaa 
      ttcatccta aacaacccg tctcaattt atttgcgac 
      tcgaagagt tcttatttg atttatggt ggaagtgcc 
      gaacaatgg acggacgca gttgaatgg ctggtgttc 
      cgaggggcc cgaaaaatc gtagtttcg tccgattct 
      aaaccccaa caagtttac gtcaatcgg cgcctttcc 
      cttcttcag agttgttac tctgctgat attatttat 
      gccccgcct aaggctcac accaaggaa ggtgccacc 
      gaactattg actgaagtg tatcacttg ggccccatc 
      cattgtgta ttctcacta ccggtgaag tcaaatgat 
      tcaagaatc agcaaaatc aagccaatc cagtacatt 
      gataacgca ttaagaact atcgccccc aaagcaatt 
      ctgatcaat ttcgtgaac cacgccgct ggaatttgc 
      caattccgg accgaagcc aacttcccc tcctaccac 
      atccagtgg cacaaggac ttggacttt ggcgaaata 
      atctacgga ctggacgag attttgacg tacaatgtg 
      cgcgatatt ctgatcaag aacgacaga gttagcgat 
      tcgcaccaa gaaaccact cagtcactt ttcaaaaaa 
      ttgactcta attttgccc aactctttg gaggacttt 
      atcgaggaa acaaaatcg gccccagct aagccacaa 
      ctgatccaa ctgcgcacg ctgggcccc cgtgaaatc 
      agggaactt gcgcccgtt tttattatc cctggactt 
      tcaggtttc aaggaaatg aacgaaatg gccttccag 
      ctgttgtac cccacgttt tgtgccgtc ttaccttcg 
      actcccatg cccatcaaa gagctggcc cttgatttg 
      gtcgaacaa ataaaaaaa gtgtggccg aaaggcgcc 
      tacaacatc attggcgtt tcctggggg ggcgccctt 
      atggtcgaa atcgccaaa attttggac aagcaagga 
      gccagcctc cacctctat ttcattgac gcggccccc 
      acaacttta caagccgct gttaaacac ttgggcgaa 
      agcgccaca agcattgag acgaacttg ctaacccgt 
      gttttgaaa attaacgat gctgaggtt gttagaaag 
      atcgaagag gcgcctggt tgggattcg cgcattaaa 
      ctggctttg gaccacttc gagggccat ttgtccgac 
      aaaaaggcg ctggggcaa ggactgttc accttgaag 
      tcgcacttg caaaacttg ttaacgttc agcccgtct 
      gaggaatta gtttcgggg caaattcat ctaatcaga 
      ccgaccggc acgagcaaa tatgataat tgcggatta 
      gtttcgtat tgcaagcaa acgccgcag attactttg 
      gtcaatggc gatcacttg agcattatt aatagtcca 
      gtgactacg gaatacatc aacgagaag cattatttg 
      atttaa
    mRNA sequence
    • >TC000238 RA
      tagtcatta caccacaaa aattttcta ttattgtgt 
      gtgcggtct gaccatgca tttcgtaaa agacgacgt 
      tgtcatttc cggcattgg cggttactt ccccaaagc 
      tttgaacat cgaaaaatt gcaagaaca cctcctcaa 
      caatgagaa tctcctaga gagccgctg gaaggccgg 
      cgagcgcgg cgtttccaa taaaatcgg tgtcgtccc 
      aactgaata tttcgacaa tgcttattt cggaattca 
      tcgtcagca gtgcacttt catggaccc catgcaccg 
      actcatcct tgaacgcac ctacgaagc gctcattga 
      cgccggtgt taacccgag cgaggttcg cggtaaaag 
      aatcggttg tttcatggc ttcgtctct cggagagaa 
      cgataattt gtttctgga gtctgtggt gagcgggtt 
      tggtgtcac tggacactc tagggctat gatgccaaa 
      cagggtgtc ctactggct ggaccttaa agggccaag 
      tgttgcgta tgatagtaa ctggattgg aggtataga 
      agtggtaag gttggccta tgaagctat caaaacagg 
      tcaatgtga ggcggtttt ggtcggaac cgctaattt 
      ggcccttaa ttcggagtt ccaatggtt gtataatga 
      catgggtct gttatcggc tgatggtag tactagggc 
      gtacgatat tgatgccca gggttatgc acgaagcga 
      tggggttgt cgttttgta catccaacg tgccagtga 
      cgctaaacg ttcctacgc cagcattgt caatgttgc 
      cactcaatt tgacggcaa ccgggaagg aactctctt 
      agatataga ctctaataa catggcaga attcattag 
      cgactttta caaggacat tgacgttga tccgaaaga 
      cgttgagtt tgttgaaac gtacggaag cgcccttaa 
      agaaaccga tcgtaaaga actagaagc gttagaaaa 
      agtctattg caaaaaccg ccaaaaccc tctccttat 
      cggcaccgt caagcccaa caccggtca cagcgaagc 
      ttctgccgc gcttttcag cattgtcaa agtttgtat 
      agccatgga ggccgagac aattccggc aacaatcca 
      gtaccaaac gccaaatcc cgaattaaa accgctcct 
      taacgggag tatggaagt ggtgacaca gaatcgtaa 
      atggggggt taaatatgc cgcagttaa tgcgattgg 
      tttggattc gtactatgg acacctcct tttgaaagc 
      caatcccaa aaagaaggt caatgctca agttcagga 
      cgatattcc aagactcat acttgcgtc cactcgaac 
      tgaggctgg gattaagga aattttgca aactatcaa 
      gtcgcgcgc aacgattga tcctgaata tgttaaact 
      catccagga tttgtattc taaaccgat tttggggca 
      cttgtaccg cggttatac gattttggg ggctgaggc 
      cccgaaaga ggaaactga gtaccattt gggcaacaa 
      gcgccaaat ttggttcgt ctactctgg gatgggcag 
      ccaatggag cggcatggc cagtgactt gatgaaact 
      cccagtttt tgcaaacgc aatccacaa atgccacaa 
      agtattagt cgacaaagg catcaactt gcttgaaat 
      catcaccag taaagacaa gaccatgtt cgacaacat 
      tttgcactc atttgtggg aatcgccgc aattcaaat 
      tgccttaac cgacgtttt gcgggctgt tggtatcgt 
      cccagatgg tataatcgg gcattccgt gggcgagct 
      agggtgtgc atacgccga taattgcat gacggccga 
      acaaatgat tttatcggc ctactcccg cgggagagc 
      ctcacttga agccaccct aatccgcgg catgatggc 
      tgccatcgg catggggta ccaacaaat caaagacaa 
      gtgcccccc gtccataga agtcgcgtg tcacaatgg 
      ccccgacag ctccacaat ctcgggccc caccgaaga 
      catggaaaa gttcgtcaa ggagttgca agaccaggg 
      gattttcgc ccgtcttgt caatgtttc caacattgc 
      ctaccacag caggtacat caaacccgc tgctccagg 
      cctcttaaa atacctcaa ggaggtgct ccccgaacc 
      cgtggcccg ctcctcaaa gtggatcag tacctccaa 
      cccggagga aaactggga gacggaact ggccaaaca 
      ctcctcggc cgaatatca caccaacaa tctcctgag 
      ctcggtttt gttcgaaga gggcttgaa acacatccc 
      taaggactc ggttttgat cgaaatcgc cccacatgg 
      gctgctcca agccatttt gaaacgctc ggtcaagtc 
      aggttgtac caacgtttc cctgactca gagaggctc 
      aaacagcgg ggttgaatt cctactttc ggcgttcgg 
      taaattgta cttggctgg tttagatat ggacatttc 
      taagctgtt cccgccggt tgagtaccc agtaagtcg 
      gggaacccc ctgtttggc gaacttatc gcattggga 
      gcacagcga agtttggag aactgggct tgaggagaa 
      actgaaatc gctgttcgg ggtgcgtga ctaccaagt 
      cacgctgaa cagcgagga gttccgaga gtgtgtggg 
      gcaccaact tgacgacaa aatcatact accgtgcac 
      ctcatactt gagcaccat cttcgacat tgtcgcaaa 
      cataagcac tgggcacaa ggaattcat ctttgagaa 
      tctccactt taagaaaat gttaaccgt cccgagaat 
      cggttcggt ggcgcttca cgcaatgat ccagaaggg 
      aagcggcga ttttgagat aatcgccgg gaacgacat 
      tctcgtaac tggaacttt gacattccc gaacccggc 
      cgataaatt catgattga tccaatcaa agtggaggt 
      gtctgacga caacgtccg cttatccgg gacagacgt 
      ttacaacga attccaaca ccggggaca caaatacag 
      cggtcagta caaatccat caagagtct agtcgtgtg 
      cgaaaacgg ctcagtcag cctggcaca gtggaacaa 
      caaatggac catgtttgt ggaagccat gatccaaca 
      acacttgtt ccaagacgg cgaacgctc gcagcaaat 
      ctacgtccc gaaaaacat tcacaaaat cgtcgtgaa 
      caaagagct tttgcctaa tgataaaac cgacctgga 
      ggtgcatta cgattttgc gaccgggct gatcacctg 
      tgacgggat ccaggtggt tgggcttaa ggctgtgcc 
      cttccccaa ggacccgaa ggcaatttc gtttgattc 
      aattgagtt tactccctt taatttgtc caacttgag 
      tattgagaa cggaattaa tctcgctct tcaactcac 
      tttggacaa cttttccga gcaatttgt taccacaat 
      cagcatcaa tgaaatcga gggcaagaa cccgcctct 
      tttggaaaa catccagaa cgtgtgctc catgtactc 
      gaaacttaa cagcaccgt aacgtcagt caaagaccc 
      ccaaactat agacttaca agtctccta ccccttttt 
      cgtcgtttt aaacgacac aattaacga cgaagtcgc 
      aaaattaac atcatcatc acacgggtt tttgttagt 
      gcgttcaag caagcaaat cctggcgta ccctcagat 
      cgcacaagt ggcgcaatt tagtgttaa cggagttga 
      atactcaat tttgcgccg tgctaaaac cacccaacc 
      gctcattgt cacagtgaa aggcgacac tttaagcat 
      caaagactt gacaagaac ttcagcttc atgggttaa 
      tgaattaga tacggctgc ggaagcagc caaagccaa 
      gaaaaatag tgtttatct ggtctcgtc tttagtccc 
      gaccgaagg catgcgcaa ctttgttga agagttgct 
      ctcaacacc taaaatgtc caacgttcg ggtgctttt 
      cgccctgga cagacgtat cggaaacgt taatgttaa 
      agaccccca gttgcaaga aatcttcaa acttgactt 
      ggttttgac ggttattaa ggacgggac tttgggctc 
      ggtcttgcc agtgccact gaaactcaa aaccaacat 
      ccagaacaa tttcggcat cactagcaa tgcgatcaa 
      ggataaagt catcaatta tgtcggtat taatttgaa 
      agacgagac tattttgcc agtcagtga gaagaaaaa 
      agagttggg taatattga ttattccgg ggttggaac 
      aaacgggca aaaagtgat gggtcttgc ccagcttga 
      cacagactt gtgccaact ggtccctga ctcaatttt 
      gtcctggaa cgttcctga taaatggag tttggagga 
      agctgcaac catcccgca cgcctacgt tagcgctta 
      ttactgcct gtttgagaa agcctggct gaaatcagg 
      cgagatcgt cctagttca tgccggttg tacggccat 
      cggcttagc tgcaatttc gatagctgc cagttatga 
      ttgtaaaat ttacaccac cgtagccac cgaatggca 
      aagaagttt cctcaaaaa acaattcga ttgcttgaa 
      agacgagaa tattttcag tttggagga ttcaagctt 
      cgagccttt gattctaat ggcaactgg tggtgttgg 
      ggctcatgt tattattaa ttgcctctc tggacatct 
      cctccaatc gtcagtcgg ttgtctggc cgagtacgg 
      gagactgat tcagattgg tcagcacga ttgtgagga 
      aaataacac aatcgggat gagcgtttt tttgaaaaa 
      tacctcgtt ctttgtggt caaacttga gaatatttt 
      cttggaatc gaacgaggt taagcagga gattaggga 
      tatggttca gaagggcat tgagagtaa aactgtgag 
      gccaattaa tcgaaaagt cgtggagca ttacaacat 
      tgtggagat tttaaattc gctaagtca gtccggaaa 
      cgtgggcaa aatcttgat caaaatcaa caacaactt 
      gctaatgaa caaattcat cctaaacaa cccgtctca 
      atttatttg cgactcgaa gagttctta tttgattta 
      tggtggaag tgccgaaca atggacgga cgcagttga 
      atggctggt gttccgagg ggcccgaaa aatcgtagt 
      ttcgtccga ttctaaacc ccaacaagt ttacgtcaa 
      tcggcgcct ttcccttct tcagagttg ttactctgc 
      tgatattat ttatgcccc gcctaaggc tcacaccaa 
      ggaaggtgc caccgaact attgactga agtgtatca 
      cttgggccc catccattg tgtattctc actaccggt 
      gaagtcaaa tgattcaag aatcagcaa aatcaagcc 
      aatccagta cattgataa cgcattaag aactatcgc 
      ccccaaagc aattctgat caatttcgt gaaccacgc 
      cgctggaat ttgccaatt ccggaccga agccaactt 
      cccctccta ccacatcca gtggcacaa ggacttgga 
      ctttggcga aataatcta cggactgga cgagatttt 
      gacgtacaa tgtgcgcga tattctgat caagaacga 
      cagagttag cgattcgca ccaagaaac cactcagtc 
      acttttcaa aaaattgac tctaatttt gcccaactc 
      tttggagga ctttatcga ggaaacaaa atcggcccc 
      agctaagcc acaactgat ccaactgcg cacgctggg 
      cccccgtga aatcaggga acttgcgcc cgtttttat 
      tatccctgg actttcagg tttcaagga aatgaacga 
      aatggcctt ccagctgtt gtaccccac gttttgtgc 
      cgtcttacc ttcgactcc catgcccat caaagagct 
      ggcccttga tttggtcga acaaataaa aaaagtgtg 
      gccgaaagg cgcctacaa catcattgg cgtttcctg 
      ggggggcgc ccttatggt cgaaatcgc caaaatttt 
      ggacaagca aggagccag cctccacct ctatttcat 
      tgacgcggc ccccacaac tttacaagc cgctgttaa 
      acacttggg cgaaagcgc cacaagcat tgagacgaa 
      cttgctaac ccgtgtttt gaaaattaa cgatgctga 
      ggttgttag aaagatcga agaggcgcc tggttggga 
      ttcgcgcat taaactggc tttggacca cttcgaggg 
      ccatttgtc cgacaaaaa ggcgctggg gcaaggact 
      gttcacctt gaagtcgca cttgcaaaa cttgttaac 
      gttcagccc gtctgagga attagtttc ggggcaaat 
      tcatctaat cagaccgac cggcacgag caaatatga 
      taattgcgg attagtttc gtattgcaa gcaaacgcc 
      gcagattac tttggtcaa tggcgatca cttgagcat 
      tattaatag tccagtgac tacggaata catcaacga 
      gaagcatta tttgattta attttttct gtaactgtt 
      tgaaataaa cgattggag gaagttgtg tttttgttg 
      tattgttgg ttgcagggg taccgtcct gtagtttga 
      ccgaaatct caaggtttt tggttatta aaaagctct 
      gaaatattg atgataaat aaatattta ataaacgtg 
      tgtgaaaaa t