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Gene information
General information
TC000234 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14410964..14412131
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000234
    iBeetle screen iB_00049

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000234 RA
      MNPFNYINL RKLRADAER LQMIDKENK QLLKSINTI 
      NRTIGKIDS YNPEAYASK SKWHAHVMR MKRIDRKNK 
      AIYEKLMAA TSSYDCESL RKFWTSIIE KLKLTSKFP 
      LVILQKVSV DSVIAKQPS ISCGLEKLS ERPRCFLEF 
      QVLNGPVLG RVEIELYHD HVPVTVQNF LSICCGENK 
      QNLSYKNCP INRIVPGRF LETGDITKG TGRGGVSIY 
      GKYFAEEGH MLKHTKPGV LSMVRVRKH DNNSRFCIT 
      FTKMEQLDM QNVVFGYIV RGAENLFKI EGYGRSIGK 
      PLAPVIISD CGKLNRALE C
    CDS sequence
    • >TC000234 RA
      atgaatccg ttcaattat atcaacctt cgaaagtta 
      cgggcagac gctgagcgg ttacaaatg attgataag 
      gagaataag cagctcctt aagtccata aatacgata 
      aataggacc atcggaaaa attgattcg tataatcct 
      gaagcctat gcgtctaaa tcgaaatgg cacgctcac 
      gttatgagg atgaagaga attgaccgc aaaaataaa 
      gcaatatac gagaaactg atggctgca acttccagt 
      tacgattgt gagtcgtta cgtaaattc tggaccagt 
      attattgaa aaattgaaa ctaacttcc aaattccct 
      ttggtcatc cttcagaaa gtctcagtc gattcagtt 
      attgcaaaa cagccgtca attagttgc ggacttgag 
      aaattatca gaacggccc cggtgtttt ttggaattt 
      caagtgcta aatggccca gttctgggc cgcgtagag 
      atcgagctt tatcacgac cacgtccct gtcacagtc 
      cagaatttc ttatcaatt tgctgcggt gagaacaaa 
      cagaacctt tcgtataaa aactgccca atcaatcga 
      atagttccg ggccgtttt ttggaaact ggggacata 
      actaaaggg acaggacgt ggtggggtg tcaatctat 
      ggtaaatac tttgcagag gaaggccac atgcttaag 
      cacacgaaa cctggagtt ttgtccatg gtgcgtgtc 
      cgcaaacac gataacaac tcgcgcttt tgtatcacc 
      ttcacgaaa atggaacag ttggatatg cagaacgtt 
      gtgttcggt tatattgtg cgaggggct gagaatttg 
      tttaaaatt gaggggtat ggccgcagt atcggcaag 
      cctttagcc cctgtaatc atctcggac tgtgggaaa 
      ttgaatcgg gcgcttgag tgttag
    mRNA sequence
    • >TC000234 RA
      aaccgaaaa ctgatacca aacccctcc ccatgaatc 
      cgttcaatt atatcaacc ttcgaaagt tacgggcag 
      acgctgagc ggttacaaa tgattgata aggagaata 
      agcagctcc ttaagtcca taaatacga taaatagga 
      ccatcggaa aaattgatt cgtataatc ctgaagcct 
      atgcgtcta aatcgaaat ggcacgctc acgttatga 
      ggatgaaga gaattgacc gcaaaaata aagcaatat 
      acgagaaac tgatggctg caacttcca gttacgatt 
      gtgagtcgt tacgtaaat tctggacca gtattattg 
      aaaaattga aactaactt ccaaattcc ctttggtca 
      tccttcaga aagtctcag tcgattcag ttattgcaa 
      aacagccgt caattagtt gcggacttg agaaattat 
      cagaacggc cccggtgtt ttttggaat ttcaagtgc 
      taaatggcc cagttctgg gccgcgtag agatcgagc 
      tttatcacg accacgtcc ctgtcacag tccagaatt 
      tcttatcaa tttgctgcg gtgagaaca aacagaacc 
      tttcgtata aaaactgcc caatcaatc gaatagttc 
      cgggccgtt ttttggaaa ctggggaca taactaaag 
      ggacaggac gtggtgggg tgtcaatct atggtaaat 
      actttgcag aggaaggcc acatgctta agcacacga 
      aacctggag ttttgtcca tggtgcgtg tccgcaaac 
      acgataaca actcgcgct tttgtatca ccttcacga 
      aaatggaac agttggata tgcagaacg ttgtgttcg 
      gttatattg tgcgagggg ctgagaatt tgtttaaaa 
      ttgaggggt atggccgca gtatcggca agcctttag 
      cccctgtaa tcatctcgg actgtggga aattgaatc 
      gggcgcttg agtgttagt ggttttcgg ggcgtcatg 
      ttgtcgttt ggaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY