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Gene information
General information
TC000221 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid11: 34574..71128
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000221
    iBeetle screen iB_00046
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000221 RA
      MKSLPPTIA IFFGTFFVC YPGCSANLP PVFSMDINN 
      LALSEKTPV GSVVYTLEG SDPENSTVH FNLEGTDVL 
      KVNRNTGAV TVVKPLDYE TNSTLNVIV SIEDEVEGG 
      RNNMVSVPA NIIILDEND NAPDFIDAP YDILAHEDT 
      TVGTTIFSK IFVEDKDTT GANLEVECI NLPEYQNAC 
      DFFQVETLQ SAQNSYHGA IVLRRKVNY TQQDKHQFL 
      LKATDGELS SSTSVTVNI GDVQDTPPR FIGNLVAEV 
      SENATINSL VMTIHAEDG DRGVPRKIV YELINNPMD 
      YFILDAVTG ELRTARPLD KEAVNNPDG ILTLNVKAH 
      ELVDGIKGT DPSTVTNAE VLIKIKDVN DEPPAFNKR 
      EYFVQIPEN ISEGSPLPG LEMVVTDPD EGNNSVFSL 
      QLNDISSAF SIEPKTVIG SSAVTIRVA NSSLDYEDL 
      NQRKFIILA LAKELYTEE KLSSTATVT VSVSDINDN 
      APTFDQDGY STAISEVSS PGTPIITIT ARDRDSGKF 
      GEDGIFYHI SGSGSERFV VHNRTGTVA VAECEHPGK 
      KPCLDFEDK AEYHLQFIA TDDDGKGQT TKVPLKILL 
      TDNNDNAPV FTQSIYRAF INEAAVRFD PDLIIEAVD 
      ADKTSHVTY SIISGNDEG LFSIEPNLG KIRISNSKG 
      VDVSNDTEN VILLTVMAS DGTFTTTSL VNITIRDVN 
      NNHPIFTKE NYVVAIAED VTIGSSIIQ LQANDMDTG 
      SNAEIEYSI QKGAYDDFQ IDNTTGVVV VASKLDFDR 
      RNTYNIEII ARDHGEPPL TGTTTLTAN IINTNDKMP 
      YFVPTTQKA EIMEDAPIG SVVHTLIAL DPDVNSTEA 
      LNFAATEPI TALDKHGNE VFNDDVYRG FFSVDKTTG 
      KVTVLNKLQ RDVAAIVRI TVLVTDITA PTVQQGEGL 
      LIITITDVN DSPPVFLPP WTPETPLYT LELKEEQPV 
      GTIVATYKA IDDGSDIAG YAILPENEY FEINNGTGI 
      VQIRKQIDY EKTPQLNFT ILAFDSGIP QLNTSASVF 
      VKVINLNDN DPIFSEKNY KASIYENSP NGTFVVKVK 
      ATDADSGDY GKLNYELTG EHSENFEIT PDTGVITVA 
      NSNFLDHEK INETVIQVV ASDGAPHGF KRSVTVPVN 
      INILDVNDN APKFNQSEY NVTVIENVR LNPPQPLLQ 
      VNATDEDSG INGNIHYDI IGGNDNDVF LLGVETGIL 
      YPHKSLLGQ KRNFHLVIE ARDGGGNGQ LFDRAVINV 
      QVLNVNEHR PDFIIPFLP NATVEITEN AAVENYLVM 
      TVKATDKDE NENGRITYH LKVDGENVQ ETNEFSIDA 
      NTGELRSKK FLDREETSK YELVLVARD HGSPKWFET 
      LRYLTILLV DTNDNRPEF PDSHSANPY HFYVTENDK 
      SGVRIGQVK ALDRDEGNH AKVYYYFLR GNENGDFRL 
      DKTEGYLYA NKSFDRERQ DEYNLYIIA TNDADYYIS 
      PQDRETLAE ADIIHDSSI AKITVTITD LNDNVPVFE 
      QAIYYSAVN AMAEINDFV TNVTAVDPD LGPNGSLTY 
      YIKAANLYK YGSNKSSGS IIPSPFNIT DKGEMCTAN 
      YLAENNQHR FVVDIIARE NAFPEREAY TQVHVWIFE 
      PEQLIRVIL SRPSDEVVR ERDEIVAEL SNATQSLVI 
      IDEIRYHTD DNGYKNEEW SDMYILVVD PLTQNILPV 
      PEVLKIIDS KYDFLKDYY AGFAIENVV PAFVMEKDE 
      SFDPALAAL IALLIVLFV GVITFIIVC CCLRHWVIS 
      PTDLKKKDA LIKKAIIDD LNTTENPLW IEQKLKIYE 
      EQELTMQVF NEPEQNVLG RRNSDDFIP DDNTYATIQ 
      HPNRRGSAH IASRSLADD LADYATLSG VPHQTDSSH 
      SSLRGAPNF YEAAMGFQG STFQVPERM SNDDYGSYR 
      TRFKGSELT INQAGQPEY VAELI
    CDS sequence
    • >TC000221 RA
      atgaagtcg ctaccgccc actattgcc atatttttc 
      gggacgttt ttcgtttgc tatccagga tgcagtgcc 
      aatttaccc cccgtgttc tcaatggac ataaacaat 
      ttagcctta tcggaaaaa actccagta ggtagcgtc 
      gtctacact ttagaagga tcagatcct gaaaacagt 
      actgtacat tttaatctc gaaggaact gacgtttta 
      aaagttaat cgcaacacg ggagctgtt accgttgtt 
      aaacctctg gattatgag acaaattcc actttaaac 
      gtgattgtg agtattgag gatgaagtt gaaggcggc 
      agaaataac atggtgtca gttcctgcc aacataatt 
      atcctagac gagaatgat aacgctcca gattttata 
      gacgcacct tatgatatc ctagcacac gaggatacc 
      acagtcggt acgacaatt ttctccaaa atttttgtg 
      gaggacaag gacactacc ggtgccaat ttggaagta 
      gaatgcatc aacttgccc gaataccag aacgcttgc 
      gattttttc caagtggag acgctccaa tcggcccag 
      aattcctac cacggtgct atagtttta agaaggaaa 
      gtcaactac acccagcag gacaagcac cagttcctg 
      ctcaaagcc acagacgga gagctgagc agtagcacg 
      agcgttaca gttaacatc ggggatgtt caagacact 
      ccgcctcgt ttcatcggt aatttagta gcggaagtg 
      agtgaaaac gcgactatt aacagtctg gtcatgacg 
      atccatgca gaagacgga gacagaggg gttccaagg 
      aagatcgtc tatgaactc attaacaat cccatggat 
      tatttcatc ctggacgct gttacggga gagctgcgg 
      accgccaga cccctggac aaggaagcc gtcaacaat 
      ccagacggc atattaact ctaaacgtt aaggcccac 
      gagctggtg gacggcata aaaggcacc gacccgtcg 
      acggtgaca aacgccgaa gtgctgata aaaattaaa 
      gacgtgaac gacgagcca ccggccttc aacaagcgc 
      gaatacttc gtgcaaatt ccggagaac atctccgaa 
      ggttcaccg ttgccgggc ttggaaatg gtggtaacc 
      gatccggac gaaggaaac aattcggtt ttttctctc 
      caactgaac gacatatcg tctgcattt tcaatcgaa 
      ccgaaaacg gtgatcggt tcatcggcc gtcacaatc 
      cgggtcgcc aacagttcg ctggattat gaagacttg 
      aatcagagg aaattcatc attttggcg ttggccaag 
      gaactgtac acggaggaa aagttgtcg tcgacggcc 
      actgtcacg gtctcggtg agcgatatt aacgataat 
      gccccaact ttcgatcaa gacggatac tcgacggct 
      atttcggaa gtctcttcg cccggaact ccaatcata 
      acaataaca gcacgggat agagacagc gggaagttt 
      ggggaagat ggcattttt taccatatt tcaggcagt 
      ggtagcgaa cggtttgtt gttcacaac aggacgggg 
      actgtggcc gtggccgag tgcgaacat ccagggaaa 
      aaaccatgt ctggatttc gaggacaag gcggagtat 
      catttgcag tttatcgcg acggacgac gatggcaag 
      ggccagacc acgaaagtg cctttaaaa atcctcctc 
      actgataat aacgacaac gcgcccgtg ttcacccaa 
      tcgatatat cgggctttt attaacgag gcggccgtt 
      cgattcgat cccgatctt ataatcgaa gccgtggat 
      gccgacaaa acgtctcac gttacttat tcgattatt 
      tccggcaac gacgaaggc ttgtttagc atcgaaccc 
      aatttgggc aaaatccga atctcaaac agtaaagga 
      gtcgacgtc agtaacgac accgaaaac gttatttta 
      ttgactgta atggcaagt gacggtact tttacaact 
      acgtcccta gttaacatc acgatacga gacgtcaat 
      aacaaccat cccatcttt acgaaggag aattacgtt 
      gttgcgatt gccgaggat gtcactatt ggatcaagc 
      atcatccaa ttgcaagct aatgatatg gatacggga 
      tcaaacgcc gaaatcgaa tactccatt caaaaggga 
      gcttacgac gactttcaa attgataac acaacaggt 
      gttgttgtg gtcgcttcc aaactggat ttcgatcgg 
      cgcaatact tacaacatt gaaattata gcgagggat 
      cacggagag cctcctctc actggaacc acgactctg 
      acagctaac atcatcaat acaaacgac aaaatgccc 
      tactttgtg cccaccacc caaaaagcc gaaattatg 
      gaagatgct ccaatcgga agtgtagtc catacacta 
      atcgcctta gatcccgat gttaactcc acggaagct 
      ttgaatttc gctgcgact gaacccatt acagccctg 
      gataagcat gggaacgaa gtttttaat gatgatgtt 
      tatcgtggc tttttttcg gtcgataaa accaccgga 
      aaagttacg gtgttgaat aaacttcaa agggacgtc 
      gcggcgatc gtcaggatt acggtcctt gtgacggat 
      attacggcc cccaccgtg caacaagga gaaggctta 
      ttgatcatc acgatcact gacgtgaac gactctccg 
      ccggtgttt ttgccccca tggacgccc gaaacccct 
      ctttatact ctcgagctg aaagaagaa cagcccgtt 
      ggaacgatt gttgcaact tacaaagcg atcgatgat 
      ggttcagac atcgccggt tacgctatc ctccccgaa 
      aatgagtat ttcgaaatc aacaacggc actggaatc 
      gtccagatt aggaaacaa atcgactac gaaaaaacg 
      ccgcaacta aatttcaca atcctggcg ttcgattcc 
      ggtattccc caactaaat acttctgcg agtgtgttt 
      gtcaaagtg ataaatctc aacgacaac gatcccatt 
      ttctcggaa aaaaattat aaagcgagc atctatgaa 
      aactcgccg aacggtact tttgtcgtg aaagttaaa 
      gcgacagat gccgactcg ggggattac ggcaaacta 
      aattatgaa ttgacggga gagcatagc gagaatttc 
      gaaataaca cccgatact ggggttatc acagtggcc 
      aattccaac tttttggat catgagaag atcaacgag 
      acggtgatt caggtggtg gccagtgat ggggcccca 
      cacggtttt aaaagatcg gtcacggtg cccgttaat 
      atcaacatc ttggatgtt aacgataac gcgcccaaa 
      tttaatcaa agcgaatat aacgtgacc gtaatagaa 
      aacgtgaga ctcaaccct ccacagcca ttattgcaa 
      gtaaatgcc actgatgaa gatagtggc attaacgga 
      aacattcat tatgatatt attggagga aacgacaac 
      gacgtattt ttgctcggg gtcgagacg ggaatcctc 
      tacccgcat aaatccctc ttaggccaa aaaagaaac 
      tttcatctt gtcatagaa gctcgagat gggggagga 
      aacggtcaa ttatttgac cgtgcggtt ataaatgta 
      caggttctg aatgtgaac gaacatcgg cccgacttt 
      attataccg ttcctaccg aacgccaca gtcgaaata 
      accgagaac gcagctgtg gaaaattat ctagtcatg 
      acagtcaaa gcgactgat aaagacgag aacgaaaac 
      ggaagaatc acatatcat ttgaaagtg gacggggaa 
      aacgttcaa gaaacgaac gaattttcc atagacgcc 
      aacacgggc gaactgagg agcaaaaaa ttcctagac 
      cgggaggaa acatccaaa tacgagttg gttttagtt 
      gccagggac cacggttcg cccaaatgg ttcgagact 
      ttgcgctac ttaaccatt ctgttagtc gacacgaac 
      gacaaccgc ccggaattc ccggattcg cattcagcg 
      aatccgtac cacttttac gtgactgag aatgataaa 
      agtggtgtc agaatcggc caagtcaaa gctctggat 
      cgagacgaa ggaaatcac gccaaagtc tactactac 
      tttctgaga ggtaacgaa aacggcgat ttccgcctc 
      gacaagacc gagggctat ttatacgcg aacaaatcg 
      ttcgatcgg gagcgccaa gacgaatac aacttgtac 
      atcatcgcg accaacgac gcagattac tacatctcg 
      ccccaagac cgggaaacg ttggccgaa gcggacata 
      atccacgac agcagcatt gcgaaaatc acggtgacc 
      ataaccgac ctaaacgac aacgtgccc gtttttgag 
      caagcgatt tactactcg gccgtgaac gcaatggcc 
      gaaatcaac gactttgtg acgaacgta acggcggtt 
      gatccagac ttgggcccg aacggctct ctgacctac 
      tacatcaaa gcggcaaac ttgtacaaa tacggatcc 
      aacaaaagc tcaggctcg atcataccc tcacctttc 
      aacatcaca gacaagggc gagatgtgc acggccaat 
      tacttggcc gaaaacaac caacaccgc ttcgttgtc 
      gacataatc gcacgggag aacgccttc ccggaacgc 
      gaggcttac acacaagtc catgtgtgg attttcgag 
      cccgagcag ctaatccgg gtcattttg tcgcgacct 
      tccgacgaa gttgtgcga gaacgagac gaaattgtg 
      gccgaactg agcaatgcc acgcaaagt cttgtcatt 
      atcgacgaa atcaggtac cacaccgat gacaacggc 
      tacaaaaat gaggaatgg tcggatatg tacattttg 
      gtggtagac ccgctcact caaaacata ctaccggtc 
      cccgaagtc ctgaaaata attgactcg aaatacgac 
      ttcctgaag gactactac gccgggttt gccatcgaa 
      aacgtggtc ccggcgttc gtaatggag aaggacgag 
      tccttcgat cccgctctg gcggcgctt attgccctc 
      ctcattgtg ctttttgtc ggtgttata acatttata 
      atcgtttgt tgttgtttg cggcactgg gttatctca 
      cccactgat cttaagaaa aaagacgct ttgattaaa 
      aaggccata atcgacgat ttgaacacc accgaaaat 
      cccttatgg atcgaacag aaattgaaa atttacgag 
      gaacaagaa ctgacaatg caagtgttt aacgagccg 
      gaacagaac gtcttgggc cgaagaaac agcgacgat 
      tttattcct gacgataat acctatgcc acaatccag 
      cacccaaac cggcgtggc tctgcccac attgcttcg 
      agatcacta gccgatgat ttggccgat tatgcgacc 
      ttgtccgga gtgccccat cagactgac agttcgcac 
      agttcgcta agaggggcc ccaaatttt tatgaagct 
      gcaatgggc ttccaaggc tcgactttc caagtgcca 
      gagcggatg agtaacgat gattatggc tcgtaccgg 
      acgagattt aaaggctcc gaactgacc attaaccag 
      gctggacag cccgaatac gtagccgaa ctaatttag
    mRNA sequence
    • >TC000221 RA
      agttacgtt ggagccgag ctggtcccc tcttccgcc 
      ggttacacc attcttttc ttcggtagt cagccgtgg 
      cagtttgtg ttcaatggt cgagtccaa agaaatgta 
      aagtgccgg cagtgtact aaattttca ctactggct 
      actgataaa agcaaatgt agtgttaat tacagtgct 
      gtgtagttg cggtgtagt gatgataaa ggggaatat 
      tttagtcac ttttaattg gaataaccg tttgcggaa 
      agttgaagg acgatgaag tcgctaccg cccactatt 
      gccatattt ttcgggacg tttttcgtt tgctatcca 
      ggatgcagt gccaattta ccccccgtg ttctcaatg 
      gacataaac aatttagcc ttatcggaa aaaactcca 
      gtaggtagc gtcgtctac actttagaa ggatcagat 
      cctgaaaac agtactgta cattttaat ctcgaagga 
      actgacgtt ttaaaagtt aatcgcaac acgggagct 
      gttaccgtt gttaaacct ctggattat gagacaaat 
      tccacttta aacgtgatt gtgagtatt gaggatgaa 
      gttgaaggc ggcagaaat aacatggtg tcagttcct 
      gccaacata attatccta gacgagaat gataacgct 
      ccagatttt atagacgca ccttatgat atcctagca 
      cacgaggat accacagtc ggtacgaca attttctcc 
      aaaattttt gtggaggac aaggacact accggtgcc 
      aatttggaa gtagaatgc atcaacttg cccgaatac 
      cagaacgct tgcgatttt ttccaagtg gagacgctc 
      caatcggcc cagaattcc taccacggt gctatagtt 
      ttaagaagg aaagtcaac tacacccag caggacaag 
      caccagttc ctgctcaaa gccacagac ggagagctg 
      agcagtagc acgagcgtt acagttaac atcggggat 
      gttcaagac actccgcct cgtttcatc ggtaattta 
      gtagcggaa gtgagtgaa aacgcgact attaacagt 
      ctggtcatg acgatccat gcagaagac ggagacaga 
      ggggttcca aggaagatc gtctatgaa ctcattaac 
      aatcccatg gattatttc atcctggac gctgttacg 
      ggagagctg cggaccgcc agacccctg gacaaggaa 
      gccgtcaac aatccagac ggcatatta actctaaac 
      gttaaggcc cacgagctg gtggacggc ataaaaggc 
      accgacccg tcgacggtg acaaacgcc gaagtgctg 
      ataaaaatt aaagacgtg aacgacgag ccaccggcc 
      ttcaacaag cgcgaatac ttcgtgcaa attccggag 
      aacatctcc gaaggttca ccgttgccg ggcttggaa 
      atggtggta accgatccg gacgaagga aacaattcg 
      gttttttct ctccaactg aacgacata tcgtctgca 
      ttttcaatc gaaccgaaa acggtgatc ggttcatcg 
      gccgtcaca atccgggtc gccaacagt tcgctggat 
      tatgaagac ttgaatcag aggaaattc atcattttg 
      gcgttggcc aaggaactg tacacggag gaaaagttg 
      tcgtcgacg gccactgtc acggtctcg gtgagcgat 
      attaacgat aatgcccca actttcgat caagacgga 
      tactcgacg gctatttcg gaagtctct tcgcccgga 
      actccaatc ataacaata acagcacgg gatagagac 
      agcgggaag tttggggaa gatggcatt ttttaccat 
      atttcaggc agtggtagc gaacggttt gttgttcac 
      aacaggacg gggactgtg gccgtggcc gagtgcgaa 
      catccaggg aaaaaacca tgtctggat ttcgaggac 
      aaggcggag tatcatttg cagtttatc gcgacggac 
      gacgatggc aagggccag accacgaaa gtgccttta 
      aaaatcctc ctcactgat aataacgac aacgcgccc 
      gtgttcacc caatcgata tatcgggct tttattaac 
      gaggcggcc gttcgattc gatcccgat cttataatc 
      gaagccgtg gatgccgac aaaacgtct cacgttact 
      tattcgatt atttccggc aacgacgaa ggcttgttt 
      agcatcgaa cccaatttg ggcaaaatc cgaatctca 
      aacagtaaa ggagtcgac gtcagtaac gacaccgaa 
      aacgttatt ttattgact gtaatggca agtgacggt 
      acttttaca actacgtcc ctagttaac atcacgata 
      cgagacgtc aataacaac catcccatc tttacgaag 
      gagaattac gttgttgcg attgccgag gatgtcact 
      attggatca agcatcatc caattgcaa gctaatgat 
      atggatacg ggatcaaac gccgaaatc gaatactcc 
      attcaaaag ggagcttac gacgacttt caaattgat 
      aacacaaca ggtgttgtt gtggtcgct tccaaactg 
      gatttcgat cggcgcaat acttacaac attgaaatt 
      atagcgagg gatcacgga gagcctcct ctcactgga 
      accacgact ctgacagct aacatcatc aatacaaac 
      gacaaaatg ccctacttt gtgcccacc acccaaaaa 
      gccgaaatt atggaagat gctccaatc ggaagtgta 
      gtccataca ctaatcgcc ttagatccc gatgttaac 
      tccacggaa gctttgaat ttcgctgcg actgaaccc 
      attacagcc ctggataag catgggaac gaagttttt 
      aatgatgat gtttatcgt ggctttttt tcggtcgat 
      aaaaccacc ggaaaagtt acggtgttg aataaactt 
      caaagggac gtcgcggcg atcgtcagg attacggtc 
      cttgtgacg gatattacg gcccccacc gtgcaacaa 
      ggagaaggc ttattgatc atcacgatc actgacgtg 
      aacgactct ccgccggtg tttttgccc ccatggacg 
      cccgaaacc cctctttat actctcgag ctgaaagaa 
      gaacagccc gttggaacg attgttgca acttacaaa 
      gcgatcgat gatggttca gacatcgcc ggttacgct 
      atcctcccc gaaaatgag tatttcgaa atcaacaac 
      ggcactgga atcgtccag attaggaaa caaatcgac 
      tacgaaaaa acgccgcaa ctaaatttc acaatcctg 
      gcgttcgat tccggtatt ccccaacta aatacttct 
      gcgagtgtg tttgtcaaa gtgataaat ctcaacgac 
      aacgatccc attttctcg gaaaaaaat tataaagcg 
      agcatctat gaaaactcg ccgaacggt acttttgtc 
      gtgaaagtt aaagcgaca gatgccgac tcgggggat 
      tacggcaaa ctaaattat gaattgacg ggagagcat 
      agcgagaat ttcgaaata acacccgat actggggtt 
      atcacagtg gccaattcc aactttttg gatcatgag 
      aagatcaac gagacggtg attcaggtg gtggccagt 
      gatggggcc ccacacggt tttaaaaga tcggtcacg 
      gtgcccgtt aatatcaac atcttggat gttaacgat 
      aacgcgccc aaatttaat caaagcgaa tataacgtg 
      accgtaata gaaaacgtg agactcaac cctccacag 
      ccattattg caagtaaat gccactgat gaagatagt 
      ggcattaac ggaaacatt cattatgat attattgga 
      ggaaacgac aacgacgta tttttgctc ggggtcgag 
      acgggaatc ctctacccg cataaatcc ctcttaggc 
      caaaaaaga aactttcat cttgtcata gaagctcga 
      gatggggga ggaaacggt caattattt gaccgtgcg 
      gttataaat gtacaggtt ctgaatgtg aacgaacat 
      cggcccgac tttattata ccgttccta ccgaacgcc 
      acagtcgaa ataaccgag aacgcagct gtggaaaat 
      tatctagtc atgacagtc aaagcgact gataaagac 
      gagaacgaa aacggaaga atcacatat catttgaaa 
      gtggacggg gaaaacgtt caagaaacg aacgaattt 
      tccatagac gccaacacg ggcgaactg aggagcaaa 
      aaattccta gaccgggag gaaacatcc aaatacgag 
      ttggtttta gttgccagg gaccacggt tcgcccaaa 
      tggttcgag actttgcgc tacttaacc attctgtta 
      gtcgacacg aacgacaac cgcccggaa ttcccggat 
      tcgcattca gcgaatccg taccacttt tacgtgact 
      gagaatgat aaaagtggt gtcagaatc ggccaagtc 
      aaagctctg gatcgagac gaaggaaat cacgccaaa 
      gtctactac tactttctg agaggtaac gaaaacggc 
      gatttccgc ctcgacaag accgagggc tatttatac 
      gcgaacaaa tcgttcgat cgggagcgc caagacgaa 
      tacaacttg tacatcatc gcgaccaac gacgcagat 
      tactacatc tcgccccaa gaccgggaa acgttggcc 
      gaagcggac ataatccac gacagcagc attgcgaaa 
      atcacggtg accataacc gacctaaac gacaacgtg 
      cccgttttt gagcaagcg atttactac tcggccgtg 
      aacgcaatg gccgaaatc aacgacttt gtgacgaac 
      gtaacggcg gttgatcca gacttgggc ccgaacggc 
      tctctgacc tactacatc aaagcggca aacttgtac 
      aaatacgga tccaacaaa agctcaggc tcgatcata 
      ccctcacct ttcaacatc acagacaag ggcgagatg 
      tgcacggcc aattacttg gccgaaaac aaccaacac 
      cgcttcgtt gtcgacata atcgcacgg gagaacgcc 
      ttcccggaa cgcgaggct tacacacaa gtccatgtg 
      tggattttc gagcccgag cagctaatc cgggtcatt 
      ttgtcgcga ccttccgac gaagttgtg cgagaacga 
      gacgaaatt gtggccgaa ctgagcaat gccacgcaa 
      agtcttgtc attatcgac gaaatcagg taccacacc 
      gatgacaac ggctacaaa aatgaggaa tggtcggat 
      atgtacatt ttggtggta gacccgctc actcaaaac 
      atactaccg gtccccgaa gtcctgaaa ataattgac 
      tcgaaatac gacttcctg aaggactac tacgccggg 
      tttgccatc gaaaacgtg gtcccggcg ttcgtaatg 
      gagaaggac gagtccttc gatcccgct ctggcggcg 
      cttattgcc ctcctcatt gtgcttttt gtcggtgtt 
      ataacattt ataatcgtt tgttgttgt ttgcggcac 
      tgggttatc tcacccact gatcttaag aaaaaagac 
      gctttgatt aaaaaggcc ataatcgac gatttgaac 
      accaccgaa aatccctta tggatcgaa cagaaattg 
      aaaatttac gaggaacaa gaactgaca atgcaagtg 
      tttaacgag ccggaacag aacgtcttg ggccgaaga 
      aacagcgac gattttatt cctgacgat aatacctat 
      gccacaatc cagcaccca aaccggcgt ggctctgcc 
      cacattgct tcgagatca ctagccgat gatttggcc 
      gattatgcg accttgtcc ggagtgccc catcagact 
      gacagttcg cacagttcg ctaagaggg gccccaaat 
      ttttatgaa gctgcaatg ggcttccaa ggctcgact 
      ttccaagtg ccagagcgg atgagtaac gatgattat 
      ggctcgtac cggacgaga tttaaaggc tccgaactg 
      accattaac caggctgga cagcccgaa tacgtagcc 
      gaactaatt tagacttag gttgataaa aatatctgt 
      gactagtgc gaaagtgat gacaatagt gatttatga 
      agggactgc tatgtaaat accgtttga tcgattttt 
      ttccacttt gtgttacat tggcggttc tttctgaat 
      caagacagt ttttctacg agtgatatc tacaaagtt 
      tctattcaa agttacgta ccgttacct gaatactga 
      gtagattat ctgactgga tctggaacc ctgttattc 
      gattgtgac gtaactatt tcttaaatt aatcagcga 
      ttttttagt tagggttaa ccaacacat actgagggc 
      catatcagt ttttacgtt ttctagacc gttgtattg 
      cgttaatgt taggaaaga atgtctttt tatttattt 
      ttttactgt aattattga atgaataga ttttacaca 
      aactggaac ttgtctttc atttcaaaa aaacaccac 
      gaaaaggct gatcacgtt ttatttata ttgcttaca 
      caaggagtt tttatttat tgtaatgca tagtaatat 
      acggaccct acaatgtaa gattattta aataaagga 
      ttgaacgtc aaagtattt ttaccctaa ttagccgtt 
      acaaagaat tatagtcta aataagata atacgttgc 
      aaaatgggt acagtcaca atcaaaacc cctaccata 
      gtttcaata aactatttt gtttacc