Status
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Gene information
General information
TC000219 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14538666..14541703
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000219
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00044

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupation not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupa lethality as pupa - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000219 RA
      MLSLVRQCR AFTPAKQFL GVSNRTLPK NGLVRTYMT 
      PIWRRQPLT NLLKTRNFL KPSGAIIVR LNSNIPQKS 
      KKSIGYWLM TCSGMVFVA VVLGGVTRL TESGLSMVT 
      WKLLGEKMP RTEKEWQEE FEKYKQFPE FKIKNREMT 
      LSEFKWIWH MEFGHRMWG RAIGAVFLI PAVYFWARG 
      RFTPAMKKR VLAFGTLIG AQGLMGWYM VKSGLEDRF 
      HGESDVPRV SQYRLAAHL SLAFVLYTL FLWSGLDQL 
      LPAETITTG CKNAIKAAR KFRMVAHTC KGLVFLTAL 
      SGAFVAGLD AGLVYNSFP KMADKWIPD DILALMPAM 
      SNFTENPTT VQFDHRVLG TTTLTVITA MYLMSRRRL 
      LPRRAYTAA AVFGALAWL QVALGITTL LTYVPTALA 
      ASHQSGSLL LLSSAVWLT HELKRLPK
    CDS sequence
    • >TC000219 RA
      atgttaagt ttagtgcga caatgtcgc gcattcacc 
      cctgctaaa caatttctg ggtgtctca aatcggacg 
      ctaccaaaa aatggactt gttaggact tacatgacc 
      cccatttgg aggaggcaa cccctaaca aatcttctc 
      aaaacgagg aattttctc aaacctagc ggagcaatt 
      attgtgaga ctgaattca aacattccg caaaaaagc 
      aaaaaaagc ataggctac tggctgatg acctgcagc 
      ggaatggtc ttcgtggcc gtagtttta ggtggcgta 
      acgcgatta accgagtca gggttgtct atggttacg 
      tggaaattg ttgggagaa aaaatgccc aggacggaa 
      aaagagtgg caggaggag tttgaaaaa tacaaacaa 
      tttcccgaa tttaaaatc aaaaacaga gaaatgact 
      ttatccgag ttcaaatgg atatggcac atggaattc 
      gggcaccgg atgtggggc cgtgccatc ggcgctgtt 
      ttcctaatc ccagctgtg tatttctgg gcccgtggc 
      agattcacc ccagcgatg aaaaaacga gtgttggct 
      ttcggtact ctgatcggt gcccaagga ttgatgggt 
      tggtacatg gttaagtca ggtcttgag gaccgattc 
      catggcgaa agcgacgta cctagagta tcgcaatat 
      cgacttgcc gcccacctt agccttgct tttgtttta 
      tatacactg ttcctgtgg tctggtctc gaccaactt 
      ttaccagcc gaaaccata accactggg tgcaaaaac 
      gccataaaa gcggcgaga aaattcagg atggtggcg 
      catacgtgt aaaggctta gtgtttctg actgcgctc 
      tcaggggct tttgtggcc ggtctggac gcaggttta 
      gtctataat tcgttccct aaaatggca gataagtgg 
      attcccgat gatatttta gcactaatg cctgctatg 
      agcaatttt acggaaaat ccaactacg gttcagttt 
      gatcatagg gttttaggg actacgact ttgactgtt 
      atcacagcc atgtatttg atgtcgaga cgcaggctt 
      ttgccacga agggcttac actgcggct gcagttttc 
      ggggctttg gcctggctg caggtcgct ttgggaata 
      acaacgctt ttaacgtac gtaccaacc gctttggcg 
      gcaagtcac caaagcggg tcgttactt ttgttatcg 
      tctgccgtt tggctgact catgaactc aaaaggtta 
      cctaagtag
    mRNA sequence
    • >TC000219 RA
      tcaattttt ttcagcaaa atcactcaa aatgttaag 
      tttagtgcg acaatgtcg cgcattcac ccctgctaa 
      acaatttct gggtgtctc aaatcggac gctaccaaa 
      aaatggact tgttaggac ttacatgac ccccatttg 
      gaggaggca acccctaac aaatcttct caaaacgag 
      gaattttct caaacctag cggagcaat tattgtgag 
      actgaattc aaacattcc gcaaaaaag caaaaaaag 
      cataggcta ctggctgat gacctgcag cggaatggt 
      cttcgtggc cgtagtttt aggtggcgt aacgcgatt 
      aaccgagtc agggttgtc tatggttac gtggaaatt 
      gttgggaga aaaaatgcc caggacgga aaaagagtg 
      gcaggagga gtttgaaaa atacaaaca atttcccga 
      atttaaaat caaaaacag agaaatgac tttatccga 
      gttcaaatg gatatggca catggaatt cgggcaccg 
      gatgtgggg ccgtgccat cggcgctgt tttcctaat 
      cccagctgt gtatttctg ggcccgtgg cagattcac 
      cccagcgat gaaaaaacg agtgttggc tttcggtac 
      tctgatcgg tgcccaagg attgatggg ttggtacat 
      ggttaagtc aggtcttga ggaccgatt ccatggcga 
      aagcgacgt acctagagt atcgcaata tcgacttgc 
      cgcccacct tagccttgc ttttgtttt atatacact 
      gttcctgtg gtctggtct cgaccaact tttaccagc 
      cgaaaccat aaccactgg gtgcaaaaa cgccataaa 
      agcggcgag aaaattcag gatggtggc gcatacgtg 
      taaaggctt agtgtttct gactgcgct ctcaggggc 
      ttttgtggc cggtctgga cgcaggttt agtctataa 
      ttcgttccc taaaatggc agataagtg gattcccga 
      tgatatttt agcactaat gcctgctat gagcaattt 
      tacggaaaa tccaactac ggttcagtt tgatcatag 
      ggttttagg gactacgac tttgactgt tatcacagc 
      catgtattt gatgtcgag acgcaggct tttgccacg 
      aagggctta cactgcggc tgcagtttt cggggcttt 
      ggcctggct gcaggtcgc tttgggaat aacaacgct 
      tttaacgta cgtaccaac cgctttggc ggcaagtca 
      ccaaagcgg gtcgttact tttgttatc gtctgccgt 
      ttggctgac tcatgaact caaaaggtt acctaagta 
      ggcgagaaa aaacgtagt tgtaatgta acaaacttt 
      tattgtaag gttattgat atttgattg ataattaat 
      atcgacact accatttat tttaaaaca aaatatata 
      tcaatatca cgtgatcca ccataaaac aaactgtta 
      ttttactta tttggtgcg tatttccat aaaaaacac 
      ttctggttt caaacttat tgcgtttta tttacatta 
      aattgccta atctcatta ttggtttag tttcgacag 
      tgactatgt ttacacggg gtttgaatt tttcctcac 
      gccccaagc aacattatc agctacagt cttttatcg 
      tattttaat tagtgagta ttacggccc gacttgtta 
      tttattccg tgtttttta actacactg atatgtgca 
      aatgaaact aacttttga ttgaagctt gaaaattcg 
      aggcttttc cagtgtagt cgatgattt gtactaagt 
      gaaaacctt cggacggac taacatttt gaccaaaaa 
      ataacaagt gggccgatt aagtaaact gtttgacac 
      cgaggtgta caaaaatag actttccag tcagctgat 
      ctaacaagt gatattagc agtcatgga attaaaaat 
      cttatttag aaaaactta catattata caaaatatc 
      aacatgtaa agaaggaat gaatttata cgtaaatct 
      tgtacgtct cgagttact tgccataat agacatacg 
      tcattcata agaagagac tttgacaaa ttcatccca 
      ttcatcgca taaaaaatt ataataata gcaaattaa 
      attatataa tacaaaagc aaagtccag tcgataaag 
      cagtataaa ttctaaact gtacaataa ctaaaaaca 
      atatagtca gtcaaaaaa tataaatgc atacaagtt 
      ttgacctaa gataagcct aaaaaaatc cgcattttt 
      gtatgcaaa aaacgtgag gcggcgaat aatttaatt 
      attgttttg cttcaatat aaaacttca ctcttaaat 
      caacctcta atgatttct attatgata tgaatagtc 
      tttttgtag taacatcat gaaacactg tattatttt 
      ggttccaag aaataattg tttacccac ttcttccaa 
      caggaatct tcagtagtt gacagtgag gttactatg 
      ggaggagaa caaacacca aatcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY