Status
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Gene information
General information
TC032264
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000201,TC000199
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14826820..14880942
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032264
    iBeetle screen iB_00041

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    elytra ventral not closed - Number of animals showing the phenotype: 9

    Adult morphology
    elytra surface irregular - Number of animals showing the phenotype: 9 (22 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032264 RA
      MNWFFVTVK DKFANKAVQ FARCLLAPV LVKWIRYVK 
      KSEQKKSLK DNSVSLLEV IWRDKVSPS RWLEVHVHA 
      LSHASRSSS SLGCRESRA DQQQSAPCQ LHDVRASTR 
      SHCRHPSVG MAFGTCVCL ALCAAAAAV GAAPREPAG 
      EPCQPDKLT VYKVVLHTF WSRDKFPKH YPDWRPPAQ 
      WSKVFGRSH NKSFTLFRL GQKSSPGVK AFAETGRSD 
      VLEEQSQGE GGIYDEFNA PPITSGVGR TEAEFFVDG 
      NHSRVSLMT RIIPSPDWF IGIDSFDLC VNGNWLDSI 
      TIEVDPIDA GTDNGFTFT APNWPTEPQ GEAYRITAH 
      YPAHPAGSF FYPYLKRLP PIGTFQFIK VKEYELSEV 
      FHHAEDDQR YEVLKMEHL TQNSINVLN GNSDIETAI 
      EEEREEQEI HQRVRPRPR PYKQFLLST STTTSATSR 
      ASSDPDRSL NSINERDDS VTSPSIPAV VPRGDKDAI 
      INSIADSYL SHSKDHHHK KYRKPRKNI RKYRPPRDC 
      RVSEWSPWS ACSKSCGIG EMQRRREVL KHARRGGRL 
      CPPLVETKW CGSARSCNK EYFKF
    CDS sequence
    • >TC032264 RA
      atgaactgg ttctttgtc actgtaaag gataagttt 
      gcaaacaaa gctgtgcaa tttgcgcgt tgcttgttg 
      gccccagtc ctcgtcaag tggatcaga tacgtcaag 
      aagagcgaa caaaagaag tctttaaaa gacaattct 
      gtgagtttg ttggaagtc atctggaga gacaaagtc 
      agtccctca cgttggttg gaggttcac gttcacgca 
      ctgagtcac gcatcacgc agcagcagc agcctaggc 
      tgcagagag agcagagca gatcagcag cagagtgcg 
      ccgtgtcaa ttgcacgac gttcgcgct tcaaccagg 
      tcgcactgt cggcatccc agtgtcggt atggctttc 
      gggacgtgt gtgtgtctg gcactctgt gcggcggcg 
      gcggcagtg ggggcggcg cccagggaa cccgcggga 
      gagccctgc cagcccgac aagctcacc gtttacaaa 
      gtggtcctg cacaccttt tggtccagg gataaattc 
      ccaaagcac taccccgac tggaggcct ccggcacag 
      tggtccaaa gttttcggt cgaagccac aacaagtcg 
      ttcactttg ttccggctg ggccagaag tcatcacct 
      ggagtgaaa gcgttcgct gagacgggc cgctcggac 
      gtcctagag gagcaaagt caaggcgaa ggaggaatt 
      tacgatgag ttcaacgct cctcccatc acatcaggc 
      gtcggcagg accgaagcg gaattcttc gtcgatgga 
      aaccattca agggtttcg cttatgaca cgaattatt 
      ccatcacca gattggttt attgggatt gacagcttt 
      gatctgtgc gtgaacggt aattggcta gacagcatc 
      acaatcgag gtggatccg attgatgcc ggaaccgac 
      aacggattc acgttcaca gcccccaac tggccgacg 
      gaaccgcaa ggggaggcc tatcgcatt acggcccat 
      taccccgct catcctgct ggatcgttc ttctatccc 
      tatctgaag cgcctgccg cccatcggc acctttcaa 
      tttattaag gtcaaagag tacgagctg agcgaagtg 
      ttccatcat gccgaagac gaccagcgc tatgaagtt 
      ttaaaaatg gagcacttg acccaaaac agcataaac 
      gttttaaac ggaaacagc gatattgag acagcaatc 
      gaagaagaa cgcgaagaa caggaaatt catcagcgt 
      gttcgacct cgaccccgt ccatataaa caattcctt 
      ctatcgaca tccacgact actagtgcc acttcgaga 
      gcctcatct gatcccgat agatcactc aacagcatc 
      aacgaacga gacgactct gtgacatcc ccttcgatt 
      cccgccgtg gtccctcga ggggacaaa gacgccatc 
      ataaacagc atcgccgat tcatatctc agtcattcg 
      aaggaccac caccataaa aagtaccgc aagcctcgg 
      aagaacatc aggaaatac cgtccgccg cgggactgc 
      cgcgtgagc gaatggtct ccgtggagc gcgtgcagc 
      aaatcctgc ggaatcggc gagatgcag cgtcgccgg 
      gaggtgcta aagcacgcc agacgcggc ggccgcctg 
      tgtccgccc ttagtcgag accaaatgg tgcggatcg 
      gcgcgctcc tgcaacaag gagtacttc aagttttag
    mRNA sequence
    • >TC032264 RA
      gcgctctcc ttgctcata aatcccaca ttcaactaa 
      cccttttga agcgctaat aactcctcg acaaaaagt 
      gagttttca atttttgat gaactggtt ctttgtcac 
      tgtaaagga taagtttgc aaacaaagc tgtgcaatt 
      tgcgcgttg cttgttggc cccagtcct cgtcaagtg 
      gatcagata cgtcaagaa gagcgaaca aaagaagtc 
      tttaaaaga caattctgt gagtttgtt ggaagtcat 
      ctggagaga caaagtcag tccctcacg ttggttgga 
      ggttcacgt tcacgcact gagtcacgc atcacgcag 
      cagcagcag cctaggctg cagagagag cagagcaga 
      tcagcagca gagtgcgcc gtgtcaatt gcacgacgt 
      tcgcgcttc aaccaggtc gcactgtcg gcatcccag 
      tgtcggtat ggctttcgg gacgtgtgt gtgtctggc 
      actctgtgc ggcggcggc ggcagtggg ggcggcgcc 
      cagggaacc cgcgggaga gccctgcca gcccgacaa 
      gctcaccgt ttacaaagt ggtcctgca caccttttg 
      gtccaggga taaattccc aaagcacta ccccgactg 
      gaggcctcc ggcacagtg gtccaaagt tttcggtcg 
      aagccacaa caagtcgtt cactttgtt ccggctggg 
      ccagaagtc atcacctgg agtgaaagc gttcgctga 
      gacgggccg ctcggacgt cctagagga gcaaagtca 
      aggcgaagg aggaattta cgatgagtt caacgctcc 
      tcccatcac atcaggcgt cggcaggac cgaagcgga 
      attcttcgt cgatggaaa ccattcaag ggtttcgct 
      tatgacacg aattattcc atcaccaga ttggtttat 
      tgggattga cagctttga tctgtgcgt gaacggtaa 
      ttggctaga cagcatcac aatcgaggt ggatccgat 
      tgatgccgg aaccgacaa cggattcac gttcacagc 
      ccccaactg gccgacgga accgcaagg ggaggccta 
      tcgcattac ggcccatta ccccgctca tcctgctgg 
      atcgttctt ctatcccta tctgaagcg cctgccgcc 
      catcggcac ctttcaatt tattaaggt caaagagta 
      cgagctgag cgaagtgtt ccatcatgc cgaagacga 
      ccagcgcta tgaagtttt aaaaatgga gcacttgac 
      ccaaaacag cataaacgt tttaaacgg aaacagcga 
      tattgagac agcaatcga agaagaacg cgaagaaca 
      ggaaattca tcagcgtgt tcgacctcg accccgtcc 
      atataaaca attccttct atcgacatc cacgactac 
      tagtgccac ttcgagagc ctcatctga tcccgatag 
      atcactcaa cagcatcaa cgaacgaga cgactctgt 
      gacatcccc ttcgattcc cgccgtggt ccctcgagg 
      ggacaaaga cgccatcat aaacagcat cgccgattc 
      atatctcag tcattcgaa ggaccacca ccataaaaa 
      gtaccgcaa gcctcggaa gaacatcag gaaataccg 
      tccgccgcg ggactgccg cgtgagcga atggtctcc 
      gtggagcgc gtgcagcaa atcctgcgg aatcggcga 
      gatgcagcg tcgccggga ggtgctaaa gcacgccag 
      acgcggcgg ccgcctgtg tccgccctt agtcgagac 
      caaatggtg cggatcggc gcgctcctg caacaagga 
      gtacttcaa gttttaggg cagcgcggg cccttcctg 
      ggagggagt tgtgcattg attggggca cacctcagt 
      gttcagtgc tcgcgccta tgaactgcc attggagac 
      actcaatgt ttgtgatca actctcagg gttcacttg 
      ttgaactct agcgcggac tgtacccaa agattgttt 
      ggatacatg cagcaattt tgtaagtat ttttaacga 
      taataatta tttttaaga agagacaat cgatattta 
      ctgacatcc aaaatgatc gaacgccca ttttgagga 
      aacaagacg tccttatac agttttcag tcagacttc 
      gtttacaat aatagggtt acgtgtagg tacttgtac 
      ttttttcaa ggagattgt gtaaagttt cttacataa 
      ttttgtgtg tgatacgcc gttttgcta atttttaag 
      gataactcg ctaaattat ttagaaatt tgtaaatat 
      aactatagg tacaaagcc gtagctgtt agaaaagaa 
      atgtcaatg aaatttcta tcaaaaatt tgtaataag 
      aactgttga tttttactt tttaatgtc aatatcatt 
      cagccagga atggtcgct cagcgtcaa accaaacat 
      gacttctac aactaggca taggataat tttgtatca 
      cataagttg tatgtatgt ataattgta ctaatatta 
      taattttta gttacttgt catgctcat atgtattca 
      ataaaacaa taaacgtta tatttacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY