| General information | |||
| TC000196 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 14900355..14904642 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000196 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00040 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
pupation delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC000196 RA
MENEDSETA DSEEVKSRA VVKYSVPKP AQFIRLKCN TDLNLPPSN WLSSSADSY GLQHVLYHQ KGFSSFRMA HMFPDCVGE VDVVSDAEN IKNLLKIPY CKNHISMMV HRVENTLLL DDFDIYKHL LRTSETEWK WLKEFFFEN VNHETYEED KNMYIKNRR RETLQQKSL VSKFLYHSL AEGDTNENT GYEDPKTLP VPLHGPQLP EPVSATPDP KPFDHNYNR NVVWTFEDI EMLIGTDLP IFGGGTHPC ISLRLRDVS KPINVLTGI DYWLDNLMS NVPEVVMCY HLNGIVQKY ELIKTEDLP RLDNSKFSP KLIRDVAQS ILSFLKSNA TKAGHTYWL FKGKDEEVV KLYDLTSLC SEKDVENGQ NPFTIPVGM LLYRVARNM KHTSDRQPG TIRMLLKNC VKLLPEEKY PEVVTSSHY MLADLYVPA GTNPEAPGL EQEVDENDE FDDDDLIDD KDDKATKIL VLDQSDSRE KFVNYYKSP PPITGTVEE RCLQAVQHV ATGLNCLQY FQQHQTSAS GKKTAEEEE VPVAKPFEP IPMPYAKLD SNDGQKKQK KKDKKKKNE KKSENALLL KNNTQPLPT WQNGTGNIS WKDHLKTLL YEKAVLVYA ILSEQHFGG RNYGNSLRT IGLLARCQL VMNRLLYNS NALKENCLL GRAGDCCIM MIQNWDKVD SYRQQFQNN SEEDLKLME QLEKDEQLY NIHIGESNM KCVFIYDIR TIEQILLKG VECYEVALK SSENDSILR RLGNGLNEV ASFYLNKAK SAKTSDLTV EMCKKAEPY LTRGLIVFE KVKDEANVA LLYTNMGHM HRLLAHANT PENRGELSQ KEKLHYNKA FINYKKALQ VLGDRKHCP GIWDAVRWE LSTAFFNMG TIMHENPPS NISKAEAEK EVIEVLQKA LLYCDFDEA NPKYPLYQY RAAMIHYRI GCLYHSHIW TAESDSTNR KNIIQLAKI NYEKASKYY LLASDAISY FTSQMQRVA LFEYLGDTA NAPNIKIKH LQYCLSVFI EINDMVKLV VEKKVEVPE DADGEKEGF KSCYALLNL IKQRLQHVL KMLVKLCLT KPPVNKDCP KLGEVYKSC YMVTFDLKD GVTRDEFVE KLHIVLTQI CDKIAEFNK TLD
- >TC000196 RA
atggaaaat gaagatagt gaaacggcc gacagtgag gaggtcaag tcacgggcc gttgtgaag tattccgtt ccaaaaccg gcccaattc attcgatta aaatgtaac accgactta aacctaccc ccttcaaat tggttgagt agttcggcc gattcttac ggcttacag cacgttctc taccaccag aagggattt tcaagtttc agaatggcc cacatgttc cctgactgt gtgggagaa gtagatgtg gtttcggat gcggaaaac atcaaaaat ctgctcaaa ataccctac tgcaaaaac cacatcagt atgatggtt cacagagtt gaaaacact ctcctactt gatgatttt gatatttac aaacattta ttgagaacc tccgaaact gaatggaag tggctcaaa gagttcttt ttcgaaaat gtcaaccat gagacttac gaagaggac aagaatatg tacattaaa aatcgaaga cgggaaact ttacaacag aaaagtcta gtttcgaaa tttctgtat cacagtttg gccgaaggt gatactaat gaaaatacg ggttatgag gacccgaaa acccttcct gtaccttta cacggcccc caattgcct gaacctgtc tccgcaaca ccagacccc aaaccgttt gatcacaat tacaacaga aatgttgtt tggactttt gaggacatt gaaatgtta ataggtacg gatttaccg atattcggt ggtggcacc cacccttgc atctcactc cgtctccga gacgtcagt aaaccaatc aacgtgcta acaggcatt gattattgg ctagacaat ttaatgtca aacgttccc gaagtcgta atgtgttac cacttgaac ggcatcgtc caaaaatac gaacttatc aaaaccgaa gacttaccc cgcttagat aattcaaaa ttttccccc aaattaatc cgagacgtt gctcaaagc attttatcg tttcttaag tcaaacgcg accaaagcc ggccacact tattggcta ttcaaggga aaagacgaa gaggttgtg aaattatac gacttgacc tcactttgc tcagagaaa gatgtggaa aatggtcaa aaccccttc actatacct gtgggtatg ttattatac cgagtggcg cgtaatatg aaacataca tcggatcgg caacccgga acaatccgg atgttgctt aaaaactgt gttaagttg ctaccggag gaaaaatac ccagaagtg gtcacgtcc tcgcactat atgttggcc gatttgtac gtcccggca gggaccaat cccgaagcg cctggttta gagcaggaa gtggatgaa aatgatgag tttgacgat gacgatttg atcgacgat aaagacgat aaagctacg aaaattctc gtcttggat cagagtgat tctagggag aaatttgtt aattattac aagtcgcca ccgccgatt acgggaact gtggaagag cggtgtctt caagctgta caacatgtg gcaactgga cttaattgc ttacaatac ttccaacaa caccaaact agtgctagt ggtaaaaaa acagccgaa gaggaggaa gttcctgtt gccaagcct tttgaaccg atcccaatg ccgtacgcc aagcttgac tcaaatgat ggtcaaaag aaacaaaaa aagaaagac aaaaaaaag aagaacgag aaaaagagt gaaaatgcc ctacttttg aaaaacaac actcagcct ttgccgact tggcaaaac gggacggga aatatctca tggaaggac catctcaag actttactt tatgaaaaa gcggtcttg gtttatgca attttgtcg gaacaacat ttcggtggg cgaaattac ggtaacagt ttgaggacg attggcttg ttggctaga tgtcagttg gtgatgaac cgcctcctt tacaattcc aacgccttg aaggagaat tgtttgctg ggcagagcc ggcgattgt tgcattatg atgatccaa aactgggat aaagtcgat agttaccgc caacaattc caaaacaat tccgaggaa gatttaaaa ttgatggaa cagctcgaa aaagacgag caattgtac aacatccac atcggcgag tcgaacatg aagtgcgtt ttcatttac gatattcgc acaatcgag caaattctt ctcaaaggt gtggagtgt tacgaagtg gcgttaaaa tcaagcgaa aatgatagt attttacgg cggttgggt aacggttta aatgaagtc gcctcgttt tatttgaat aaagccaag tcggctaaa acgagcgat cttactgtg gaaatgtgc aaaaaagcc gaaccgtac ttaacgaga ggtttgatt gttttcgaa aaggttaaa gatgaggcg aatgttgca cttttgtac acaaatatg ggtcatatg catcgactt ttagcacat gctaatacg ccagaaaat cggggcgag ttgagtcag aaagaaaag ttgcactat aataaggca tttattaat tataagaag gcgttgcaa gttttgggg gatcggaaa cactgtcct gggatttgg gatgctgtt aggtgggag ctctcgact gcgtttttc aatatgggg acgattatg cacgaaaat ccgccctct aatatctcg aaagccgag gcggaaaag gaggtgatt gaagtgttg caaaaagcg ctcctttat tgcgacttt gacgaagca aatcccaag tatccgctg tatcaatac cgcgcagcg atgattcat tatcggatt ggttgtctt tatcacagt cacatctgg acggctgaa tccgattcg acaaacaga aaaaacatt atacaatta gcgaagatt aattacgag aaagcttcc aaatattat ctcctcgct tcggatgca attagttac tttacgtcg caaatgcaa cgagtggca ctgtttgag tatctcgga gatactgct aatgcgccg aatatcaag attaaacat ctgcagtac tgtttgagt gtgtttatt gagatcaat gatatggtt aagcttgtg gttgagaag aaagtcgag gtgccggag gatgcagat ggggagaaa gaaggcttt aaaagttgt tacgctttg ctgaatctc ataaagcaa cgattacaa cacgtactt aaaatgctg gtcaagtta tgtttgacc aaacctcca gttaacaaa gactgtccg aaattaggg gaagtttac aaaagttgt tacatggta acgtttgac ttgaaagat ggagtcaca cgtgatgag tttgtggaa aaattacac attgttctt acacaaatt tgcgacaag atcgccgag tttaacaaa actttggat tag
- >TC000196 RA
tgtcaaaga aattttaaa agtcgttca caaaaattc aaccttgca aaaaatcca aaaacggga agattataa acactcaac acactcgtg cattacatt agtaacccg cccattatg gaaaatgaa gatagtgaa acggccgac agtgaggag gtcaagtca cgggccgtt gtgaagtat tccgttcca aaaccggcc caattcatt cgattaaaa tgtaacacc gacttaaac ctaccccct tcaaattgg ttgagtagt tcggccgat tcttacggc ttacagcac gttctctac caccagaag ggattttca agtttcaga atggcccac atgttccct gactgtgtg ggagaagta gatgtggtt tcggatgcg gaaaacatc aaaaatctg ctcaaaata ccctactgc aaaaaccac atcagtatg atggttcac agagttgaa aacactctc ctacttgat gattttgat atttacaaa catttattg agaacctcc gaaactgaa tggaagtgg ctcaaagag ttctttttc gaaaatgtc aaccatgag acttacgaa gaggacaag aatatgtac attaaaaat cgaagacgg gaaacttta caacagaaa agtctagtt tcgaaattt ctgtatcac agtttggcc gaaggtgat actaatgaa aatacgggt tatgaggac ccgaaaacc cttcctgta cctttacac ggcccccaa ttgcctgaa cctgtctcc gcaacacca gaccccaaa ccgtttgat cacaattac aacagaaat gttgtttgg acttttgag gacattgaa atgttaata ggtacggat ttaccgata ttcggtggt ggcacccac ccttgcatc tcactccgt ctccgagac gtcagtaaa ccaatcaac gtgctaaca ggcattgat tattggcta gacaattta atgtcaaac gttcccgaa gtcgtaatg tgttaccac ttgaacggc atcgtccaa aaatacgaa cttatcaaa accgaagac ttaccccgc ttagataat tcaaaattt tcccccaaa ttaatccga gacgttgct caaagcatt ttatcgttt cttaagtca aacgcgacc aaagccggc cacacttat tggctattc aagggaaaa gacgaagag gttgtgaaa ttatacgac ttgacctca ctttgctca gagaaagat gtggaaaat ggtcaaaac cccttcact atacctgtg ggtatgtta ttataccga gtggcgcgt aatatgaaa catacatcg gatcggcaa cccggaaca atccggatg ttgcttaaa aactgtgtt aagttgcta ccggaggaa aaataccca gaagtggtc acgtcctcg cactatatg ttggccgat ttgtacgtc ccggcaggg accaatccc gaagcgcct ggtttagag caggaagtg gatgaaaat gatgagttt gacgatgac gatttgatc gacgataaa gacgataaa gctacgaaa attctcgtc ttggatcag agtgattct agggagaaa tttgttaat tattacaag tcgccaccg ccgattacg ggaactgtg gaagagcgg tgtcttcaa gctgtacaa catgtggca actggactt aattgctta caatacttc caacaacac caaactagt gctagtggt aaaaaaaca gccgaagag gaggaagtt cctgttgcc aagcctttt gaaccgatc ccaatgccg tacgccaag cttgactca aatgatggt caaaagaaa caaaaaaag aaagacaaa aaaaagaag aacgagaaa aagagtgaa aatgcccta cttttgaaa aacaacact cagcctttg ccgacttgg caaaacggg acgggaaat atctcatgg aaggaccat ctcaagact ttactttat gaaaaagcg gtcttggtt tatgcaatt ttgtcggaa caacatttc ggtgggcga aattacggt aacagtttg aggacgatt ggcttgttg gctagatgt cagttggtg atgaaccgc ctcctttac aattccaac gccttgaag gagaattgt ttgctgggc agagccggc gattgttgc attatgatg atccaaaac tgggataaa gtcgatagt taccgccaa caattccaa aacaattcc gaggaagat ttaaaattg atggaacag ctcgaaaaa gacgagcaa ttgtacaac atccacatc ggcgagtcg aacatgaag tgcgttttc atttacgat attcgcaca atcgagcaa attcttctc aaaggtgtg gagtgttac gaagtggcg ttaaaatca agcgaaaat gatagtatt ttacggcgg ttgggtaac ggtttaaat gaagtcgcc tcgttttat ttgaataaa gccaagtcg gctaaaacg agcgatctt actgtggaa atgtgcaaa aaagccgaa ccgtactta acgagaggt ttgattgtt ttcgaaaag gttaaagat gaggcgaat gttgcactt ttgtacaca aatatgggt catatgcat cgactttta gcacatgct aatacgcca gaaaatcgg ggcgagttg agtcagaaa gaaaagttg cactataat aaggcattt attaattat aagaaggcg ttgcaagtt ttgggggat cggaaacac tgtcctggg atttgggat gctgttagg tgggagctc tcgactgcg tttttcaat atggggacg attatgcac gaaaatccg ccctctaat atctcgaaa gccgaggcg gaaaaggag gtgattgaa gtgttgcaa aaagcgctc ctttattgc gactttgac gaagcaaat cccaagtat ccgctgtat caataccgc gcagcgatg attcattat cggattggt tgtctttat cacagtcac atctggacg gctgaatcc gattcgaca aacagaaaa aacattata caattagcg aagattaat tacgagaaa gcttccaaa tattatctc ctcgcttcg gatgcaatt agttacttt acgtcgcaa atgcaacga gtggcactg tttgagtat ctcggagat actgctaat gcgccgaat atcaagatt aaacatctg cagtactgt ttgagtgtg tttattgag atcaatgat atggttaag cttgtggtt gagaagaaa gtcgaggtg ccggaggat gcagatggg gagaaagaa ggctttaaa agttgttac gctttgctg aatctcata aagcaacga ttacaacac gtacttaaa atgctggtc aagttatgt ttgaccaaa cctccagtt aacaaagac tgtccgaaa ttaggggaa gtttacaaa agttgttac atggtaacg tttgacttg aaagatgga gtcacacgt gatgagttt gtggaaaaa ttacacatt gttcttaca caaatttgc gacaagatc gccgagttt aacaaaact ttggattag ctgaccctg tattttatt tttcgttat gattgttaa gtccaaact tgtttaaat attgaagtg attgcggaa aaatccaat cagtttcgg tattagaat aaatacttg tttttgtac ctgttgcgt gtacgttaa gttggtcag tcgtttttg tattgtttt tttttacca ttcctgtca tattttagc gagacttta ttgcattat tgcgttgct cgaaacgtc agtgaatat gtgatctaa tcaaactcg tggttgatt caatctact tttctgtga taggtcagt gtattctat aataaacta tttttatgc gt
