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Gene information
General information
TC000196 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid11: 443758..448045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000196
    iBeetle screen iB_00040
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupation delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000196 RA
      MENEDSETA DSEEVKSRA VVKYSVPKP AQFIRLKCN 
      TDLNLPPSN WLSSSADSY GLQHVLYHQ KGFSSFRMA 
      HMFPDCVGE VDVVSDAEN IKNLLKIPY CKNHISMMV 
      HRVENTLLL DDFDIYKHL LRTSETEWK WLKEFFFEN 
      VNHETYEED KNMYIKNRR RETLQQKSL VSKFLYHSL 
      AEGDTNENT GYEDPKTLP VPLHGPQLP EPVSATPDP 
      KPFDHNYNR NVVWTFEDI EMLIGTDLP IFGGGTHPC 
      ISLRLRDVS KPINVLTGI DYWLDNLMS NVPEVVMCY 
      HLNGIVQKY ELIKTEDLP RLDNSKFSP KLIRDVAQS 
      ILSFLKSNA TKAGHTYWL FKGKDEEVV KLYDLTSLC 
      SEKDVENGQ NPFTIPVGM LLYRVARNM KHTSDRQPG 
      TIRMLLKNC VKLLPEEKY PEVVTSSHY MLADLYVPA 
      GTNPEAPGL EQEVDENDE FDDDDLIDD KDDKATKIL 
      VLDQSDSRE KFVNYYKSP PPITGTVEE RCLQAVQHV 
      ATGLNCLQY FQQHQTSAS GKKTAEEEE VPVAKPFEP 
      IPMPYAKLD SNDGQKKQK KKDKKKKNE KKSENALLL 
      KNNTQPLPT WQNGTGNIS WKDHLKTLL YEKAVLVYA 
      ILSEQHFGG RNYGNSLRT IGLLARCQL VMNRLLYNS 
      NALKENCLL GRAGDCCIM MIQNWDKVD SYRQQFQNN 
      SEEDLKLME QLEKDEQLY NIHIGESNM KCVFIYDIR 
      TIEQILLKG VECYEVALK SSENDSILR RLGNGLNEV 
      ASFYLNKAK SAKTSDLTV EMCKKAEPY LTRGLIVFE 
      KVKDEANVA LLYTNMGHM HRLLAHANT PENRGELSQ 
      KEKLHYNKA FINYKKALQ VLGDRKHCP GIWDAVRWE 
      LSTAFFNMG TIMHENPPS NISKAEAEK EVIEVLQKA 
      LLYCDFDEA NPKYPLYQY RAAMIHYRI GCLYHSHIW 
      TAESDSTNR KNIIQLAKI NYEKASKYY LLASDAISY 
      FTSQMQRVA LFEYLGDTA NAPNIKIKH LQYCLSVFI 
      EINDMVKLV VEKKVEVPE DADGEKEGF KSCYALLNL 
      IKQRLQHVL KMLVKLCLT KPPVNKDCP KLGEVYKSC 
      YMVTFDLKD GVTRDEFVE KLHIVLTQI CDKIAEFNK 
      TLD
    CDS sequence
    • >TC000196 RA
      atggaaaat gaagatagt gaaacggcc gacagtgag 
      gaggtcaag tcacgggcc gttgtgaag tattccgtt 
      ccaaaaccg gcccaattc attcgatta aaatgtaac 
      accgactta aacctaccc ccttcaaat tggttgagt 
      agttcggcc gattcttac ggcttacag cacgttctc 
      taccaccag aagggattt tcaagtttc agaatggcc 
      cacatgttc cctgactgt gtgggagaa gtagatgtg 
      gtttcggat gcggaaaac atcaaaaat ctgctcaaa 
      ataccctac tgcaaaaac cacatcagt atgatggtt 
      cacagagtt gaaaacact ctcctactt gatgatttt 
      gatatttac aaacattta ttgagaacc tccgaaact 
      gaatggaag tggctcaaa gagttcttt ttcgaaaat 
      gtcaaccat gagacttac gaagaggac aagaatatg 
      tacattaaa aatcgaaga cgggaaact ttacaacag 
      aaaagtcta gtttcgaaa tttctgtat cacagtttg 
      gccgaaggt gatactaat gaaaatacg ggttatgag 
      gacccgaaa acccttcct gtaccttta cacggcccc 
      caattgcct gaacctgtc tccgcaaca ccagacccc 
      aaaccgttt gatcacaat tacaacaga aatgttgtt 
      tggactttt gaggacatt gaaatgtta ataggtacg 
      gatttaccg atattcggt ggtggcacc cacccttgc 
      atctcactc cgtctccga gacgtcagt aaaccaatc 
      aacgtgcta acaggcatt gattattgg ctagacaat 
      ttaatgtca aacgttccc gaagtcgta atgtgttac 
      cacttgaac ggcatcgtc caaaaatac gaacttatc 
      aaaaccgaa gacttaccc cgcttagat aattcaaaa 
      ttttccccc aaattaatc cgagacgtt gctcaaagc 
      attttatcg tttcttaag tcaaacgcg accaaagcc 
      ggccacact tattggcta ttcaaggga aaagacgaa 
      gaggttgtg aaattatac gacttgacc tcactttgc 
      tcagagaaa gatgtggaa aatggtcaa aaccccttc 
      actatacct gtgggtatg ttattatac cgagtggcg 
      cgtaatatg aaacataca tcggatcgg caacccgga 
      acaatccgg atgttgctt aaaaactgt gttaagttg 
      ctaccggag gaaaaatac ccagaagtg gtcacgtcc 
      tcgcactat atgttggcc gatttgtac gtcccggca 
      gggaccaat cccgaagcg cctggttta gagcaggaa 
      gtggatgaa aatgatgag tttgacgat gacgatttg 
      atcgacgat aaagacgat aaagctacg aaaattctc 
      gtcttggat cagagtgat tctagggag aaatttgtt 
      aattattac aagtcgcca ccgccgatt acgggaact 
      gtggaagag cggtgtctt caagctgta caacatgtg 
      gcaactgga cttaattgc ttacaatac ttccaacaa 
      caccaaact agtgctagt ggtaaaaaa acagccgaa 
      gaggaggaa gttcctgtt gccaagcct tttgaaccg 
      atcccaatg ccgtacgcc aagcttgac tcaaatgat 
      ggtcaaaag aaacaaaaa aagaaagac aaaaaaaag 
      aagaacgag aaaaagagt gaaaatgcc ctacttttg 
      aaaaacaac actcagcct ttgccgact tggcaaaac 
      gggacggga aatatctca tggaaggac catctcaag 
      actttactt tatgaaaaa gcggtcttg gtttatgca 
      attttgtcg gaacaacat ttcggtggg cgaaattac 
      ggtaacagt ttgaggacg attggcttg ttggctaga 
      tgtcagttg gtgatgaac cgcctcctt tacaattcc 
      aacgccttg aaggagaat tgtttgctg ggcagagcc 
      ggcgattgt tgcattatg atgatccaa aactgggat 
      aaagtcgat agttaccgc caacaattc caaaacaat 
      tccgaggaa gatttaaaa ttgatggaa cagctcgaa 
      aaagacgag caattgtac aacatccac atcggcgag 
      tcgaacatg aagtgcgtt ttcatttac gatattcgc 
      acaatcgag caaattctt ctcaaaggt gtggagtgt 
      tacgaagtg gcgttaaaa tcaagcgaa aatgatagt 
      attttacgg cggttgggt aacggttta aatgaagtc 
      gcctcgttt tatttgaat aaagccaag tcggctaaa 
      acgagcgat cttactgtg gaaatgtgc aaaaaagcc 
      gaaccgtac ttaacgaga ggtttgatt gttttcgaa 
      aaggttaaa gatgaggcg aatgttgca cttttgtac 
      acaaatatg ggtcatatg catcgactt ttagcacat 
      gctaatacg ccagaaaat cggggcgag ttgagtcag 
      aaagaaaag ttgcactat aataaggca tttattaat 
      tataagaag gcgttgcaa gttttgggg gatcggaaa 
      cactgtcct gggatttgg gatgctgtt aggtgggag 
      ctctcgact gcgtttttc aatatgggg acgattatg 
      cacgaaaat ccgccctct aatatctcg aaagccgag 
      gcggaaaag gaggtgatt gaagtgttg caaaaagcg 
      ctcctttat tgcgacttt gacgaagca aatcccaag 
      tatccgctg tatcaatac cgcgcagcg atgattcat 
      tatcggatt ggttgtctt tatcacagt cacatctgg 
      acggctgaa tccgattcg acaaacaga aaaaacatt 
      atacaatta gcgaagatt aattacgag aaagcttcc 
      aaatattat ctcctcgct tcggatgca attagttac 
      tttacgtcg caaatgcaa cgagtggca ctgtttgag 
      tatctcgga gatactgct aatgcgccg aatatcaag 
      attaaacat ctgcagtac tgtttgagt gtgtttatt 
      gagatcaat gatatggtt aagcttgtg gttgagaag 
      aaagtcgag gtgccggag gatgcagat ggggagaaa 
      gaaggcttt aaaagttgt tacgctttg ctgaatctc 
      ataaagcaa cgattacaa cacgtactt aaaatgctg 
      gtcaagtta tgtttgacc aaacctcca gttaacaaa 
      gactgtccg aaattaggg gaagtttac aaaagttgt 
      tacatggta acgtttgac ttgaaagat ggagtcaca 
      cgtgatgag tttgtggaa aaattacac attgttctt 
      acacaaatt tgcgacaag atcgccgag tttaacaaa 
      actttggat tag
    mRNA sequence
    • >TC000196 RA
      tgtcaaaga aattttaaa agtcgttca caaaaattc 
      aaccttgca aaaaatcca aaaacggga agattataa 
      acactcaac acactcgtg cattacatt agtaacccg 
      cccattatg gaaaatgaa gatagtgaa acggccgac 
      agtgaggag gtcaagtca cgggccgtt gtgaagtat 
      tccgttcca aaaccggcc caattcatt cgattaaaa 
      tgtaacacc gacttaaac ctaccccct tcaaattgg 
      ttgagtagt tcggccgat tcttacggc ttacagcac 
      gttctctac caccagaag ggattttca agtttcaga 
      atggcccac atgttccct gactgtgtg ggagaagta 
      gatgtggtt tcggatgcg gaaaacatc aaaaatctg 
      ctcaaaata ccctactgc aaaaaccac atcagtatg 
      atggttcac agagttgaa aacactctc ctacttgat 
      gattttgat atttacaaa catttattg agaacctcc 
      gaaactgaa tggaagtgg ctcaaagag ttctttttc 
      gaaaatgtc aaccatgag acttacgaa gaggacaag 
      aatatgtac attaaaaat cgaagacgg gaaacttta 
      caacagaaa agtctagtt tcgaaattt ctgtatcac 
      agtttggcc gaaggtgat actaatgaa aatacgggt 
      tatgaggac ccgaaaacc cttcctgta cctttacac 
      ggcccccaa ttgcctgaa cctgtctcc gcaacacca 
      gaccccaaa ccgtttgat cacaattac aacagaaat 
      gttgtttgg acttttgag gacattgaa atgttaata 
      ggtacggat ttaccgata ttcggtggt ggcacccac 
      ccttgcatc tcactccgt ctccgagac gtcagtaaa 
      ccaatcaac gtgctaaca ggcattgat tattggcta 
      gacaattta atgtcaaac gttcccgaa gtcgtaatg 
      tgttaccac ttgaacggc atcgtccaa aaatacgaa 
      cttatcaaa accgaagac ttaccccgc ttagataat 
      tcaaaattt tcccccaaa ttaatccga gacgttgct 
      caaagcatt ttatcgttt cttaagtca aacgcgacc 
      aaagccggc cacacttat tggctattc aagggaaaa 
      gacgaagag gttgtgaaa ttatacgac ttgacctca 
      ctttgctca gagaaagat gtggaaaat ggtcaaaac 
      cccttcact atacctgtg ggtatgtta ttataccga 
      gtggcgcgt aatatgaaa catacatcg gatcggcaa 
      cccggaaca atccggatg ttgcttaaa aactgtgtt 
      aagttgcta ccggaggaa aaataccca gaagtggtc 
      acgtcctcg cactatatg ttggccgat ttgtacgtc 
      ccggcaggg accaatccc gaagcgcct ggtttagag 
      caggaagtg gatgaaaat gatgagttt gacgatgac 
      gatttgatc gacgataaa gacgataaa gctacgaaa 
      attctcgtc ttggatcag agtgattct agggagaaa 
      tttgttaat tattacaag tcgccaccg ccgattacg 
      ggaactgtg gaagagcgg tgtcttcaa gctgtacaa 
      catgtggca actggactt aattgctta caatacttc 
      caacaacac caaactagt gctagtggt aaaaaaaca 
      gccgaagag gaggaagtt cctgttgcc aagcctttt 
      gaaccgatc ccaatgccg tacgccaag cttgactca 
      aatgatggt caaaagaaa caaaaaaag aaagacaaa 
      aaaaagaag aacgagaaa aagagtgaa aatgcccta 
      cttttgaaa aacaacact cagcctttg ccgacttgg 
      caaaacggg acgggaaat atctcatgg aaggaccat 
      ctcaagact ttactttat gaaaaagcg gtcttggtt 
      tatgcaatt ttgtcggaa caacatttc ggtgggcga 
      aattacggt aacagtttg aggacgatt ggcttgttg 
      gctagatgt cagttggtg atgaaccgc ctcctttac 
      aattccaac gccttgaag gagaattgt ttgctgggc 
      agagccggc gattgttgc attatgatg atccaaaac 
      tgggataaa gtcgatagt taccgccaa caattccaa 
      aacaattcc gaggaagat ttaaaattg atggaacag 
      ctcgaaaaa gacgagcaa ttgtacaac atccacatc 
      ggcgagtcg aacatgaag tgcgttttc atttacgat 
      attcgcaca atcgagcaa attcttctc aaaggtgtg 
      gagtgttac gaagtggcg ttaaaatca agcgaaaat 
      gatagtatt ttacggcgg ttgggtaac ggtttaaat 
      gaagtcgcc tcgttttat ttgaataaa gccaagtcg 
      gctaaaacg agcgatctt actgtggaa atgtgcaaa 
      aaagccgaa ccgtactta acgagaggt ttgattgtt 
      ttcgaaaag gttaaagat gaggcgaat gttgcactt 
      ttgtacaca aatatgggt catatgcat cgactttta 
      gcacatgct aatacgcca gaaaatcgg ggcgagttg 
      agtcagaaa gaaaagttg cactataat aaggcattt 
      attaattat aagaaggcg ttgcaagtt ttgggggat 
      cggaaacac tgtcctggg atttgggat gctgttagg 
      tgggagctc tcgactgcg tttttcaat atggggacg 
      attatgcac gaaaatccg ccctctaat atctcgaaa 
      gccgaggcg gaaaaggag gtgattgaa gtgttgcaa 
      aaagcgctc ctttattgc gactttgac gaagcaaat 
      cccaagtat ccgctgtat caataccgc gcagcgatg 
      attcattat cggattggt tgtctttat cacagtcac 
      atctggacg gctgaatcc gattcgaca aacagaaaa 
      aacattata caattagcg aagattaat tacgagaaa 
      gcttccaaa tattatctc ctcgcttcg gatgcaatt 
      agttacttt acgtcgcaa atgcaacga gtggcactg 
      tttgagtat ctcggagat actgctaat gcgccgaat 
      atcaagatt aaacatctg cagtactgt ttgagtgtg 
      tttattgag atcaatgat atggttaag cttgtggtt 
      gagaagaaa gtcgaggtg ccggaggat gcagatggg 
      gagaaagaa ggctttaaa agttgttac gctttgctg 
      aatctcata aagcaacga ttacaacac gtacttaaa 
      atgctggtc aagttatgt ttgaccaaa cctccagtt 
      aacaaagac tgtccgaaa ttaggggaa gtttacaaa 
      agttgttac atggtaacg tttgacttg aaagatgga 
      gtcacacgt gatgagttt gtggaaaaa ttacacatt 
      gttcttaca caaatttgc gacaagatc gccgagttt 
      aacaaaact ttggattag ctgaccctg tattttatt 
      tttcgttat gattgttaa gtccaaact tgtttaaat 
      attgaagtg attgcggaa aaatccaat cagtttcgg 
      tattagaat aaatacttg tttttgtac ctgttgcgt 
      gtacgttaa gttggtcag tcgtttttg tattgtttt 
      tttttacca ttcctgtca tattttagc gagacttta 
      ttgcattat tgcgttgct cgaaacgtc agtgaatat 
      gtgatctaa tcaaactcg tggttgatt caatctact 
      tttctgtga taggtcagt gtattctat aataaacta 
      tttttatgc gt