Status
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Gene information
General information
TC000192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14922095..14927062
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000192
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00039

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000192 RA
      MSVDAYGPS SQTLTFLET EENDLIGAD TQGSECEFT 
      DFTIPSQTQ ASQHDHAGP KVSGVQLNN NINNKIANA 
      TSALGELQF EEEDEEYFN SKELPNYAC KYCGIHEPN 
      CVVMCNCCK KWFCNGRGN TSGSHIINH LVRAKHKEV 
      TLHKDGPLG ETILECYSC GVRNVFVLG FIPAKADSV 
      VVLLCRQPC AAQNSLKDM NWDQDQWKP LITDRCFLT 
      WLVKIPSEQ EQLRARQIS AQQINKLEE LWKDNVDAT 
      FQDLERPGV DEEPQQVLL RYEDGYQYQ NIFGPLVKL 
      EADYDKRLK ESQTQENIE VRWDVGLNK KTIAYFTLA 
      KTDGDMKLM HGDELRLRY VGEMHKSWA GVGHVIKVP 
      DNYGEDIGI ELKNGNGAP TDCNSNFVV DFIWKSTSF 
      DRMQLALRK FAVDDTSVS AYIYHRLLG HEVEEVLFR 
      CHLPKHFSA PNLPDLNRS QVYAVKHAL QRPLSLIQG 
      PPGTGKTVT SATIVYQLV KQNGGPVLV CAPSNTAVD 
      QLTEKIHKT NLKVVRLCA KSREAIDSP VSFLALHNQ 
      IRKMEANTE LQKLQQLKD ETGELSSVD EKRYRMLKK 
      LAEKELLEA ADVICCTCV GAGDPRLVR LKFHSILID 
      ESMQATEPE CMVPVVLGV KQLILVGDH CQLGPVVMC 
      KKAARAGLS QSLFERLVV LGIRPFRLE VQYRMHPEL 
      SRFPSNFFY EGSLQNGVC ADERKLNKI DFPWPVIDR 
      PMFFHVTQG QEEIAGSGT SYLNRTEAA NVEKIATRF 
      LRSGVKPEQ IGVITPYEG QRAYLVQYM QYQGSLHSK 
      TYQEIEIAS VDAFQGREK DIIIMSCVR SNEHQGIGF 
      LNDPRRLNV ALTRAKYGI IIVGNPKVL SKQPLWNHL 
      LSFYKEQKV LVEGPLTNL KESLIQFAK PKKLTNSDN 
      PGSHFMTTS MFDAREAMV PGSIYDRTN TAINGQFNY 
      PRGAVPLDI FSRTHDPIS YISQERAQA GMNLPVPVG 
      VFMNMADVP PRFYNQHQQ AMQARQQQN SRTRKPTMR 
      QKGKTLSQE QTQPFSQSL QLTQGMSQP GLSQPGFSL 
      SQPGLSQPE LSQDPYMAE YQSQMDGLL SQDSTYQGD 
      RSAFYQPNA QFSQPY
    CDS sequence
    • >TC000192 RA
      atgagtgtc gacgcatac ggccccagt tcgcaaact 
      ctcacgttt ttagagacc gaagaaaac gacttgatc 
      ggggctgac acccaagga tccgaatgc gaattcacc 
      gatttcaca attccatcg caaacgcaa gcctcgcag 
      cacgaccac gcgggcccc aaagtttcc ggtgtacag 
      ctcaacaac aacatcaac aacaaaatc gccaatgcg 
      acgagtgct ttaggggaa ctgcaattc gaggaagag 
      gatgaggag tatttcaac agcaaggag ctcccgaac 
      tacgcgtgc aagtactgc ggcattcat gagccgaat 
      tgcgttgtc atgtgcaac tgctgcaaa aagtggttt 
      tgtaacggg agaggaaac acctcagga agccacata 
      atcaaccat ttggtccga gccaaacac aaggaagtg 
      accttacac aaggacggc cctttgggc gagacaatc 
      cttgaatgt tacagttgt ggcgtccgc aatgttttt 
      gttttgggg ttcattcct gccaaagct gactctgtt 
      gtggtgcta ctttgccgc caaccttgt gccgcccaa 
      aactcactt aaagacatg aactgggat caagaccaa 
      tggaaacca ctcattacc gaccggtgt tttctcaca 
      tggcttgta aaaataccc tcagagcag gaacaactg 
      cgagcgcgc caaatctca gcccaacaa atcaacaaa 
      cttgaagaa ctttggaaa gataatgtt gatgccacg 
      ttccaggat ttggaacgg ccaggagtt gacgaagaa 
      ccgcaacaa gttttattg cgatatgag gacggttat 
      caataccaa aacattttt ggtcctctt gttaagctt 
      gaagctgat tatgataaa agattgaag gagtctcag 
      actcaggaa aatattgaa gttaggtgg gatgtgggt 
      ttgaataag aaaacaatt gcgtatttt actcttgca 
      aaaactgac ggggatatg aaattaatg cacggagat 
      gagttgaga cttagatac gtgggagaa atgcataaa 
      tcatgggcg ggggtggga cacgttatc aaagttcct 
      gataattac ggagaggat attggcatt gagttgaag 
      aacggaaat ggggctccc accgattgt aacagcaac 
      tttgtggtg gatttcatt tggaaaagt accagtttt 
      gacaggatg caactcgcc ttgcgaaaa ttcgccgtt 
      gacgataca tccgtttcc gcctacatc tatcaccga 
      ctcctcggc cacgaggtc gaagaggtc cttttccgt 
      tgtcacctc cctaaacat ttttcggca cctaatctt 
      cccgatttg aaccgttcg caagtctac gccgtcaaa 
      catgccctc cagcgtcca ctatccctc atccaaggt 
      cctcccgga acgggaaaa accgtaact tccgccacc 
      atcgtctat caattggtg aaacaaaac ggtggtcca 
      gtcctagtc tgtgctcct agtaatacg gcagttgac 
      caacttacc gaaaaaatt cacaaaact aatctaaaa 
      gttgtaaga ttgtgtgcg aaatcacgc gaagcgatt 
      gattcgcct gtgagtttt cttgcattg cataatcag 
      atacgtaaa atggaagcc aatactgaa ctccagaag 
      ttgcaacag ttgaaagat gagactgga gagttgagt 
      tcagttgat gagaaacga tacagaatg ttgaaaaaa 
      ttggctgag aaagaatta ctcgaagct gccgatgtt 
      atttgttgt acttgtgtg ggggcaggt gatccacga 
      ttggttcgt ttgaaattt cattcgatt ctgattgat 
      gaaagtatg caagcgact gaacccgaa tgtatggta 
      ccggtggta ctaggagtg aaacaattg attttggtt 
      ggtgatcat tgtcagttg ggtccagtt gttatgtgt 
      aagaaagca gcaagggct ggattgtcc caaagtttg 
      tttgagagg ttggtggta ttgggaatt cggccgttt 
      cgcttggaa gtacaatac aggatgcat ccagaattg 
      tcgcgtttt ccctcgaat ttcttctat gaagggagt 
      ttgcaaaat ggagtttgt gccgatgaa aggaagttg 
      aacaagatt gattttcca tggccggtt attgacagg 
      cccatgttt ttccacgtg acccaaggg caggaggaa 
      attgccggg tctgggact agttatctg aaccgaact 
      gaggcagct aatgtcgaa aagatcgcc actagattt 
      ttgcggtcg ggagttaaa ccggaacag atcggtgtt 
      ataacccca tatgaaggt cagcgagct tacttggtc 
      caatacatg cagtaccaa ggctcactt catagtaag 
      acttatcaa gaaattgaa attgcaagt gtcgatgcg 
      ttccaagga cgtgaaaaa gatataatt ataatgtca 
      tgtgtgcgt tcaaatgaa catcaagga attggattt 
      ttaaacgat cctcgacgt ctcaacgtt gcattaact 
      cgcgctaaa tacggaata ataatagtc gggaacccg 
      aaagtacta tcaaaacaa ccactctgg aaccatctt 
      ttgtcgttc tacaaagaa cagaaagtc ctagttgag 
      ggacctttg acaaacttg aaggagtcg ctgattcag 
      tttgcaaaa ccgaaaaag ctgacaaat tcggataat 
      ccgggatcg catttcatg acgacttcg atgtttgat 
      gcccgggag gcaatggta cctggctcg atttatgac 
      aggactaat actgccatc aatggccag tttaattat 
      cctcgtggg gctgtacca ttggatatc tttagtagg 
      actcacgac ccgattagt tacatttct caagagagg 
      gctcaagct ggcatgaat ttgccggtt ccagttggc 
      gtgtttatg aatatggcc gatgttccg ccacgtttt 
      tacaaccag caccagcag gccatgcaa gcgcgccaa 
      cagcagaat tcccgcact cgcaaaccg acaatgcgc 
      cagaagggc aagactttg agtcaggag cagactcag 
      ccgttcagt cagagtttg caattgact cagggaatg 
      tctcagcct gggttatcc cagcccggg ttcagtcta 
      tcgcagcct ggactgagt caaccggag ctcagtcag 
      gacccatat atggcagag tatcagtca cagatggac 
      ggcttgttg tcacaagac tcgacctat cagggagac 
      cgatcggca ttctatcag ccaaacgct cagttctcc 
      caaccatat tga
    mRNA sequence
    • >TC000192 RA
      acaccgtgc caaccaacg aaaaaaagc ttagggatt 
      aaattagac aataatgtt gtgcaataa tctatttcc 
      taaaccact ttttaattt tgaggccat aaaaaagat 
      cagtgcgct aaaaactga aaaagatag cacgagtgt 
      ttaagtttc ccgctccgt caattatgc ttcggaaag 
      agaaatgac gataaaaaa acaaatgat taatcagga 
      aaatatggc gcttgccag gagaaaaat gtagaaatt 
      ttaaagtaa ccatagtcg agttgtcat caattgaag 
      tgttaaaag tgtttcatc agtaaacgt gaataaacc 
      cgtgcttca attcgttcg cgacgaaat ctccaagat 
      gagtgtcga cgcatacgg ccccagttc gcaaactct 
      cacgttttt agagaccga agaaaacga cttgatcgg 
      ggctgacac ccaaggatc cgaatgcga attcaccga 
      tttcacaat tccatcgca aacgcaagc ctcgcagca 
      cgaccacgc gggccccaa agtttccgg tgtacagct 
      caacaacaa catcaacaa caaaatcgc caatgcgac 
      gagtgcttt aggggaact gcaattcga ggaagagga 
      tgaggagta tttcaacag caaggagct cccgaacta 
      cgcgtgcaa gtactgcgg cattcatga gccgaattg 
      cgttgtcat gtgcaactg ctgcaaaaa gtggttttg 
      taacgggag aggaaacac ctcaggaag ccacataat 
      caaccattt ggtccgagc caaacacaa ggaagtgac 
      cttacacaa ggacggccc tttgggcga gacaatcct 
      tgaatgtta cagttgtgg cgtccgcaa tgtttttgt 
      tttggggtt cattcctgc caaagctga ctctgttgt 
      ggtgctact ttgccgcca accttgtgc cgcccaaaa 
      ctcacttaa agacatgaa ctgggatca agaccaatg 
      gaaaccact cattaccga ccggtgttt tctcacatg 
      gcttgtaaa aataccctc agagcagga acaactgcg 
      agcgcgcca aatctcagc ccaacaaat caacaaact 
      tgaagaact ttggaaaga taatgttga tgccacgtt 
      ccaggattt ggaacggcc aggagttga cgaagaacc 
      gcaacaagt tttattgcg atatgagga cggttatca 
      ataccaaaa catttttgg tcctcttgt taagcttga 
      agctgatta tgataaaag attgaagga gtctcagac 
      tcaggaaaa tattgaagt taggtggga tgtgggttt 
      gaataagaa aacaattgc gtattttac tcttgcaaa 
      aactgacgg ggatatgaa attaatgca cggagatga 
      gttgagact tagatacgt gggagaaat gcataaatc 
      atgggcggg ggtgggaca cgttatcaa agttcctga 
      taattacgg agaggatat tggcattga gttgaagaa 
      cggaaatgg ggctcccac cgattgtaa cagcaactt 
      tgtggtgga tttcatttg gaaaagtac cagttttga 
      caggatgca actcgcctt gcgaaaatt cgccgttga 
      cgatacatc cgtttccgc ctacatcta tcaccgact 
      cctcggcca cgaggtcga agaggtcct tttccgttg 
      tcacctccc taaacattt ttcggcacc taatcttcc 
      cgatttgaa ccgttcgca agtctacgc cgtcaaaca 
      tgccctcca gcgtccact atccctcat ccaaggtcc 
      tcccggaac gggaaaaac cgtaacttc cgccaccat 
      cgtctatca attggtgaa acaaaacgg tggtccagt 
      cctagtctg tgctcctag taatacggc agttgacca 
      acttaccga aaaaattca caaaactaa tctaaaagt 
      tgtaagatt gtgtgcgaa atcacgcga agcgattga 
      ttcgcctgt gagttttct tgcattgca taatcagat 
      acgtaaaat ggaagccaa tactgaact ccagaagtt 
      gcaacagtt gaaagatga gactggaga gttgagttc 
      agttgatga gaaacgata cagaatgtt gaaaaaatt 
      ggctgagaa agaattact cgaagctgc cgatgttat 
      ttgttgtac ttgtgtggg ggcaggtga tccacgatt 
      ggttcgttt gaaatttca ttcgattct gattgatga 
      aagtatgca agcgactga acccgaatg tatggtacc 
      ggtggtact aggagtgaa acaattgat tttggttgg 
      tgatcattg tcagttggg tccagttgt tatgtgtaa 
      gaaagcagc aagggctgg attgtccca aagtttgtt 
      tgagaggtt ggtggtatt gggaattcg gccgtttcg 
      cttggaagt acaatacag gatgcatcc agaattgtc 
      gcgttttcc ctcgaattt cttctatga agggagttt 
      gcaaaatgg agtttgtgc cgatgaaag gaagttgaa 
      caagattga ttttccatg gccggttat tgacaggcc 
      catgttttt ccacgtgac ccaagggca ggaggaaat 
      tgccgggtc tgggactag ttatctgaa ccgaactga 
      ggcagctaa tgtcgaaaa gatcgccac tagattttt 
      gcggtcggg agttaaacc ggaacagat cggtgttat 
      aaccccata tgaaggtca gcgagctta cttggtcca 
      atacatgca gtaccaagg ctcacttca tagtaagac 
      ttatcaaga aattgaaat tgcaagtgt cgatgcgtt 
      ccaaggacg tgaaaaaga tataattat aatgtcatg 
      tgtgcgttc aaatgaaca tcaaggaat tggattttt 
      aaacgatcc tcgacgtct caacgttgc attaactcg 
      cgctaaata cggaataat aatagtcgg gaacccgaa 
      agtactatc aaaacaacc actctggaa ccatctttt 
      gtcgttcta caaagaaca gaaagtcct agttgaggg 
      acctttgac aaacttgaa ggagtcgct gattcagtt 
      tgcaaaacc gaaaaagct gacaaattc ggataatcc 
      gggatcgca tttcatgac gacttcgat gtttgatgc 
      ccgggaggc aatggtacc tggctcgat ttatgacag 
      gactaatac tgccatcaa tggccagtt taattatcc 
      tcgtggggc tgtaccatt ggatatctt tagtaggac 
      tcacgaccc gattagtta catttctca agagagggc 
      tcaagctgg catgaattt gccggttcc agttggcgt 
      gtttatgaa tatggccga tgttccgcc acgttttta 
      caaccagca ccagcaggc catgcaagc gcgccaaca 
      gcagaattc ccgcactcg caaaccgac aatgcgcca 
      gaagggcaa gactttgag tcaggagca gactcagcc 
      gttcagtca gagtttgca attgactca gggaatgtc 
      tcagcctgg gttatccca gcccgggtt cagtctatc 
      gcagcctgg actgagtca accggagct cagtcagga 
      cccatatat ggcagagta tcagtcaca gatggacgg 
      cttgttgtc acaagactc gacctatca gggagaccg 
      atcggcatt ctatcagcc aaacgctca gttctccca 
      accatattg atcacgttt acacggaga tgtttaaaa 
      aaatcgcgg gagcggtta atgaaaatt tgtaagtgg 
      ttgatctga ttgtcgatt gtcaaatta tcagtaacg 
      cagcacaca caggtccat gacgtcgaa taccgattg 
      agtaataac agagaaagt tcaatgtca tgacgcacc 
      tttgtgatg agatcagac gacttctac aatcgtgag 
      agcgcgaca tgcatctcc aagaagcct gtagatgat 
      tgttttcac acctctgct gtgacaaac tttccatat 
      ttatttcaa acattcatt tttagttat ttcgcatcg 
      ttttcatat tggtaatat tacgctctt tgtttaaac 
      ctaaattgt aacaagcat ggaagagta tttttctac 
      tttgatgga gacgcaaac acggatccc gtcttaaac 
      taacgaagt aatattatc aatacaaaa atcctagtg 
      tctatttta atctttgta aaattattg ttttagcta 
      gttattagt tattttaat tttttggta taagtaaaa 
      acacctggg tttttttgg gggatagta tatttttgt 
      ccatattaa attaatttg cttgtaaca cattaaacg 
      aaaatttaa tgtcaggtg tactaattt cgaaattcc 
      ttccctttg aacacgatt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY