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Gene information
General information
TC000188 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14939351..14943419
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000188
    iBeetle screen iB_00038

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    pupation delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Comment: 1 larva killed

    remarks: entire repetition development delayed,
    Pupal morphology
    remarks: entire repetition development delayed,
    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1

    ovary size decreased - Number of animals showing the phenotype: 2
    ovariole mostly not present - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000188 RA
      MGKVPGIGI DLGTTYSCV GVWQHGKVE IIANDQGNR 
      TTPSYVAFT DTERLIGDA AKSQVAMNP KNTVFDAKR 
      LIGRKFDDT KIQEDMKHW PFTVINDGG KPKIQVEYK 
      GEIKKFAPE EISSMVLTK MKEIADTYL GAKVNDAVI 
      TVPAYFNDS QRQATKDAG AIAGLNVLR IINEPTAAA 
      LAYGLDKNL KGEKNVLIF DLGGGTFDV SILSIDEGS 
      LFEVKSTAG DTHLGGEDF DNRLVNHFI QEFKRKHHK 
      DLSSNTRAV RRLRTACER AKRTLSSSA EASIEIDAL 
      HEGIDFYSK VSRARFEEM CMDYFRSTL QPVERALAD 
      AKLDKGAIH DIVLVGGST RIPKIQKML QDFFCGKPL 
      NLSINPDEA VAYGAAVQA AILTGDTSS QIQDVLLVD 
      VAPLSLGIE TAGGVMTKI VERNSRIPC KQQQTFTTY 
      SDNQNAVTI QVFEGERAM TKDNNLLGT FNLTGIPPA 
      PRGVPKIEV TFDLDANGI LNVSAKDTS TGKSERITI 
      TNDKGRLSK ADIDKMLAE AEKYKAEDD KQKERIAAR 
      NQLEGYIFS VKQAGEDAP ADKLTEDDK KIIREKCSA 
      ALSWLDSNQ LAEKEEFED KLKELQKDC SPIMMKLHQ 
      GAQGGAAPG AKGPTVEEV D
    CDS sequence
    • >TC000188 RA
      atgggcaaa gtaccagga attggcatc gatttgggc 
      accacgtat tcgtgtgtg ggggtgtgg cagcatggc 
      aaagtggag atcatcgcc aacgaccaa ggcaacaga 
      accaccccc agctatgtg gccttcacc gacaccgag 
      cggctcatc ggagacgcc gccaaaagt caggttgcg 
      atgaacccc aaaaacacc gtcttcgac gccaaacgc 
      ctaatcggc cgcaaattc gacgacacc aaaatccaa 
      gaagacatg aaacactgg ccattcacc gtaatcaac 
      gacggcggc aaaccaaaa atccaagtc gagtacaag 
      ggcgaaata aaaaaattt gcaccggaa gaaatcagt 
      tcgatggtt ttgaccaaa atgaaggaa attgcggac 
      acgtattta ggcgcaaaa gtcaacgat gctgttatc 
      acagttcct gcctacttc aacgactcg caaagacag 
      gccactaag gacgctggg gccatagct gggctgaac 
      gtcctgcgg attattaac gagccaacg gctgcggct 
      ctcgcctac gggctggat aaaaacttg aaaggggaa 
      aaaaacgtt ctgattttt gatctcggg ggtggaacg 
      ttcgacgtg tctattctt tcaatagac gaagggtcg 
      ctgtttgaa gttaaatca acggccggc gacacgcat 
      ttaggcggc gaagacttc gataatcgc ctagtcaac 
      catttcatc caagaattc aaacgcaaa caccacaag 
      gacttgagc agcaacacc agagcggtc cgccggctg 
      cgaactgcg tgcgaaaga gccaaaagg acactgtct 
      tcaagtgcc gaagccagt attgaaatc gacgccctg 
      catgaaggc atcgatttc tactccaaa gtcagcaga 
      gcccgcttt gaagaaatg tgcatggat tatttcaga 
      tcgacactt cagccggtt gaaagagcc ttggctgac 
      gccaagctg gacaaaggc gcaattcac gacattgtc 
      ctagtcggg ggttccacc cgtatcccg aaaatccag 
      aaaatgttg caggatttt ttctgcggg aaaccgctc 
      aacctttca atcaacccg gacgaggcc gtggcctat 
      ggagccgcc gtccaagct gcgatctta accggagac 
      accagctcg caaattcag gacgttttg ctagtcgat 
      gttgcccca ctgtctcta gggattgaa acagcgggc 
      ggtgtcatg accaaaatt gtggaacga aattcacga 
      attccgtgc aaacaacag caaactttc accacgtat 
      tctgataat cagaacgct gtcactatc caggtgttt 
      gaaggagaa cgggctatg actaaggac aataatctc 
      ctgggcact ttcaacctg actgggatc cccccagca 
      cctcgtggt gtcccaaaa atcgaagta acgttcgat 
      ttggatgcc aatggcatt ctgaacgtt tcagcaaag 
      gacacaagc accgggaag tccgaacga atcacaatt 
      acgaacgac aaaggccgt ttgtctaag gctgacatt 
      gacaaaatg ctggcagag gcggagaaa tacaaagct 
      gaggatgat aagcagaag gaacgaatt gctgcgcga 
      aaccaactg gagggttac atttttagt gtcaaacag 
      gccggtgag gacgcccca gctgataaa ctgacagaa 
      gatgataag aaaattatc agagagaag tgctccgct 
      gccttgagt tggttggac tcgaaccaa ttggccgag 
      aaggaagaa tttgaggat aagttgaaa gagttgcaa 
      aaggattgc tccccgatt atgatgaaa ctgcaccaa 
      ggagcgcaa ggaggtgca gctcccggt gccaaaggc 
      cccacggtt gaagaagta gactaa
    mRNA sequence
    • >TC000188 RA
      acatttaga gtatcggcg cttcggtgg gagcgacag 
      tcaagcatc agttgtagt tttacgaac tgctccagc 
      atcaccatg ggcaaagta ccaggaatt ggcatcgat 
      ttgggcacc acgtattcg tgtgtgggg gtgtggcag 
      catggcaaa gtggagatc atcgccaac gaccaaggc 
      aacagaacc acccccagc tatgtggcc ttcaccgac 
      accgagcgg ctcatcgga gacgccgcc aaaagtcag 
      gttgcgatg aaccccaaa aacaccgtc ttcgacgcc 
      aaacgccta atcggccgc aaattcgac gacaccaaa 
      atccaagaa gacatgaaa cactggcca ttcaccgta 
      atcaacgac ggcggcaaa ccaaaaatc caagtcgag 
      tacaagggc gaaataaaa aaatttgca ccggaagaa 
      atcagttcg atggttttg accaaaatg aaggaaatt 
      gcggacacg tatttaggc gcaaaagtc aacgatgct 
      gttatcaca gttcctgcc tacttcaac gactcgcaa 
      agacaggcc actaaggac gctggggcc atagctggg 
      ctgaacgtc ctgcggatt attaacgag ccaacggct 
      gcggctctc gcctacggg ctggataaa aacttgaaa 
      ggggaaaaa aacgttctg atttttgat ctcgggggt 
      ggaacgttc gacgtgtct attctttca atagacgaa 
      gggtcgctg tttgaagtt aaatcaacg gccggcgac 
      acgcattta ggcggcgaa gacttcgat aatcgccta 
      gtcaaccat ttcatccaa gaattcaaa cgcaaacac 
      cacaaggac ttgagcagc aacaccaga gcggtccgc 
      cggctgcga actgcgtgc gaaagagcc aaaaggaca 
      ctgtcttca agtgccgaa gccagtatt gaaatcgac 
      gccctgcat gaaggcatc gatttctac tccaaagtc 
      agcagagcc cgctttgaa gaaatgtgc atggattat 
      ttcagatcg acacttcag ccggttgaa agagccttg 
      gctgacgcc aagctggac aaaggcgca attcacgac 
      attgtccta gtcgggggt tccacccgt atcccgaaa 
      atccagaaa atgttgcag gattttttc tgcgggaaa 
      ccgctcaac ctttcaatc aacccggac gaggccgtg 
      gcctatgga gccgccgtc caagctgcg atcttaacc 
      ggagacacc agctcgcaa attcaggac gttttgcta 
      gtcgatgtt gccccactg tctctaggg attgaaaca 
      gcgggcggt gtcatgacc aaaattgtg gaacgaaat 
      tcacgaatt ccgtgcaaa caacagcaa actttcacc 
      acgtattct gataatcag aacgctgtc actatccag 
      gtgtttgaa ggagaacgg gctatgact aaggacaat 
      aatctcctg ggcactttc aacctgact gggatcccc 
      ccagcacct cgtggtgtc ccaaaaatc gaagtaacg 
      ttcgatttg gatgccaat ggcattctg aacgtttca 
      gcaaaggac acaagcacc gggaagtcc gaacgaatc 
      acaattacg aacgacaaa ggccgtttg tctaaggct 
      gacattgac aaaatgctg gcagaggcg gagaaatac 
      aaagctgag gatgataag cagaaggaa cgaattgct 
      gcgcgaaac caactggag ggttacatt tttagtgtc 
      aaacaggcc ggtgaggac gccccagct gataaactg 
      acagaagat gataagaaa attatcaga gagaagtgc 
      tccgctgcc ttgagttgg ttggactcg aaccaattg 
      gccgagaag gaagaattt gaggataag ttgaaagag 
      ttgcaaaag gattgctcc ccgattatg atgaaactg 
      caccaagga gcgcaagga ggtgcagct cccggtgcc 
      aaaggcccc acggttgaa gaagtagac taattgctc 
      gtttgcatt tatggcttg tttaattaa aatttcaat 
      aaacgaact ttgcaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY