| General information | |||
| TC000185 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 14993998..15005729 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000185 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00037 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC000185 RA
MAEGNGELS EEPMKIEGE PERTEDYQK LIEYGLDEK VAAKLDEIY KTGKLAHVD LDERALDAL KEFPVDGAL NVLGQFLDS NLEHVSNKS AYLCGVMKT YRQKSRAGS SQGTTTPAP APVKGPDEE KIKQILDRT GYTLDVTTG QRKYGGPPP GWDGSPPGA GCEVFCGKI PKDMYEDEL IPLFEGCGT IWDLRLMMD PMTGTNRGY AFVTFTTRD AAQQAVQKL DNHEIKPGK TLKINISVP NLRLFVGNI PKSKGKEEI LEEFGKLTA GLVEVIIYS SPDDKKKNR GFCFLEYES HKAASLAKR RLGTGRIKV WGCDIIVDW ADPQEEPDE QTMSKVKVL YVRNLTQEI SEEKLKEAF EAYGKVERV KKIKDYAFI HFEDRENAV KAMEELDGK EMGGSNIEV SLAKPPSDK KKKEEILRA RERRMMQMM QVRGGMMPG AMPLRGPPG QGARSGAGM RGPMGRGDY DYDYDYYGY GDYRGGYSD PYYDDYYRY EDYYYDYPT APTQSRGRG RQPQPAGRG RGVVARGRA GGPPARGAA ANRAGRGGV AGGRGAARQ TARGGVGRA KGSLPAGKR KFDGGHQNQ GETKRRYQS NWGSQPLPQ QPLGADVNG EQQAWYQDS YGSWS
- >TC000185 RB
MAEGNGELS EEPMKIEGE PERTEDYQK LIEYGLDEK VAAKLDEIY KTGKLAHVD LDERALDAL KEFPVDGAL NVLGQFLDS NLEHVSNKS AYLCGVMKT YRQKSRAGS SQGTTTPAP APVKGPDEE KIKQILDRT GYTLDVTTG QRKYGGPPP GWDGSPPGA GCEVFCGKI PKDMYEDEL IPLFEGCGT IWDLRLMMD PMTGTNRGY AFVTFTTRD AAQQAVQKL DNHEIKPGK TLKINISVP NLRLFVGNI PKSKGKEEI LEEFGKLTA GLVEVIIYS SPDDKKKNR GFCFLEYES HKAASLAKR RLGTGRIKV WGCDIIVDW ADPQEEPDE QTMSKVKVL YVRNLTQEI SEEKLKEAF EAYGKVERV KKIKDYAFI HFEDRENAV KAMEELDGK EMGGSNIEV SLAKPPSDK KKKEEILRA RERRMMQMM QVRGGMMPG AMPLRGPPG QGARSGAGM RGPMGRGDY DYDYDYYGY GDYRGGYSD PYYDDYYRY EDYYYDYPT APTQSRGRG RQPQPAGRG RGVVARGRA GGPPARGAA ANRAGRGGV AGGRGAARQ TARGGVGRA KGSLPGKRK FDGGHQNQG ETKRRYQSN WGSQPLPQQ PLGADVNGE QQAWYQDSY GSWS
- >TC000185 RA
atggccgaa ggaaatggt gaattatca gaagagcca atgaaaatt gaaggggaa cccgagaga accgaagac tatcaaaaa ttaatagaa tatggcctc gatgagaag gtggctgct aagttagac gagatttat aagactgga aaactagca catgtcgac ctcgatgag agagctctc gatgctctc aaggagttt cccgtcgat ggggccctt aacgttctg ggccagttc ctcgactcg aatttggaa catgtctcg aataaatcg gcttatctt tgcggcgtt atgaagacc tatcggcag aagagtcgc gccggttcg agtcaaggg acgacaact cctgcaccc gctcctgtc aaaggaccc gacgaagag aagatcaag cagattttg gacaggacg ggatatacg ttggatgtc accactggt caacgcaag tatggaggt cctccgcca ggatgggat gggtcgcct ccaggtgcc ggctgtgaa gtcttctgc gggaagatc ccgaaggat atgtacgag gatgaattg atcccactg tttgagggc tgcggcacg atttgggat ttgagattg atgatggac ccaatgacc ggcaccaac agagggtat gcattcgtg acgtttacg acacgtgat gctgcccag caagccgtc caaaagctc gataatcac gaaatcaaa cccggcaag accctgaaa attaacatt agtgttccg aacctccga cttttcgtg ggcaacata ccaaagtct aaaggcaaa gaggagata ctggaagag tttggtaaa ttaacagcc ggccttgtg gaagtcatc atctacagc tcaccggac gacaagaag aagaatcgc ggcttctgt ttcctagaa tatgaatcg cacaaagcc gcttcgttg gccaaacgt cgactgggc actggacga atcaaagtt tggggttgt gacatcatt gtcgattgg gctgatcca caagaagaa ccagacgaa caaaccatg tcgaaggtt aaagtcttg tacgtacga aatttaacg caagaaatc agcgaagag aaactcaaa gaagcgttc gaggcctat ggaaaagta gaacgtgtt aaaaaaatt aaagattac gcctttata catttcgaa gatcgggaa aatgccgtt aaagccatg gaagaatta gatgggaaa gagatgggc gggtccaat atagaagta tcgttagct aaacctccg tccgataaa aagaaaaag gaagaaatt ttaagagcg cgtgaacga cgtatgatg caaatgatg caagtgcgt ggaggaatg atgccaggt gctatgcct ctgagggga ccacctgga cagggagcg aggagcggt gccggtatg agaggaccg atgggccgt ggggattac gattatgat tacgactat tacgggtac ggggattat cgaggcggc tacagtgac ccatattat gatgactat taccggtat gaagattac tattatgat tatccgacg gcgccgacg caatcccga gggagaggc cggcaacca caaccggct ggccgtggg cgtggggtg gtggcacgt ggccgggcc ggtgggccc ccagcccgt ggggcggcg gccaacaga gcgggccgg ggaggagta gcagggggc cgtggggca gcacgccaa accgctcgt gggggagtg ggccgcgcc aagggaagt ttaccagca ggtaaacgg aaatttgac gggggtcac cagaaccaa ggggagaca aagcgcaga taccagagc aactggggt agccagccc ctcccgcag cagccgctg ggggcggac gtcaatgga gagcagcag gcgtggtac caggattcg tacgggtcc tggagctaa
- >TC000185 RB
atggccgaa ggaaatggt gaattatca gaagagcca atgaaaatt gaaggggaa cccgagaga accgaagac tatcaaaaa ttaatagaa tatggcctc gatgagaag gtggctgct aagttagac gagatttat aagactgga aaactagca catgtcgac ctcgatgag agagctctc gatgctctc aaggagttt cccgtcgat ggggccctt aacgttctg ggccagttc ctcgactcg aatttggaa catgtctcg aataaatcg gcttatctt tgcggcgtt atgaagacc tatcggcag aagagtcgc gccggttcg agtcaaggg acgacaact cctgcaccc gctcctgtc aaaggaccc gacgaagag aagatcaag cagattttg gacaggacg ggatatacg ttggatgtc accactggt caacgcaag tatggaggt cctccgcca ggatgggat gggtcgcct ccaggtgcc ggctgtgaa gtcttctgc gggaagatc ccgaaggat atgtacgag gatgaattg atcccactg tttgagggc tgcggcacg atttgggat ttgagattg atgatggac ccaatgacc ggcaccaac agagggtat gcattcgtg acgtttacg acacgtgat gctgcccag caagccgtc caaaagctc gataatcac gaaatcaaa cccggcaag accctgaaa attaacatt agtgttccg aacctccga cttttcgtg ggcaacata ccaaagtct aaaggcaaa gaggagata ctggaagag tttggtaaa ttaacagcc ggccttgtg gaagtcatc atctacagc tcaccggac gacaagaag aagaatcgc ggcttctgt ttcctagaa tatgaatcg cacaaagcc gcttcgttg gccaaacgt cgactgggc actggacga atcaaagtt tggggttgt gacatcatt gtcgattgg gctgatcca caagaagaa ccagacgaa caaaccatg tcgaaggtt aaagtcttg tacgtacga aatttaacg caagaaatc agcgaagag aaactcaaa gaagcgttc gaggcctat ggaaaagta gaacgtgtt aaaaaaatt aaagattac gcctttata catttcgaa gatcgggaa aatgccgtt aaagccatg gaagaatta gatgggaaa gagatgggc gggtccaat atagaagta tcgttagct aaacctccg tccgataaa aagaaaaag gaagaaatt ttaagagcg cgtgaacga cgtatgatg caaatgatg caagtgcgt ggaggaatg atgccaggt gctatgcct ctgagggga ccacctgga cagggagcg aggagcggt gccggtatg agaggaccg atgggccgt ggggattac gattatgat tacgactat tacgggtac ggggattat cgaggcggc tacagtgac ccatattat gatgactat taccggtat gaagattac tattatgat tatccgacg gcgccgacg caatcccga gggagaggc cggcaacca caaccggct ggccgtggg cgtggggtg gtggcacgt ggccgggcc ggtgggccc ccagcccgt ggggcggcg gccaacaga gcgggccgg ggaggagta gcagggggc cgtggggca gcacgccaa accgctcgt gggggagtg ggccgcgcc aagggaagt ttaccaggt aaacggaaa tttgacggg ggtcaccag aaccaaggg gagacaaag cgcagatac cagagcaac tggggtagc cagcccctc ccgcagcag ccgctgggg gcggacgtc aatggagag cagcaggcg tggtaccag gattcgtac gggtcctgg agctaa
- >TC000185 RA
cgactagac ggcttgcaa aacaacgga atctgcaga gtgcacatt gcattacac cgcgggagt cggtatact acatcaaaa cccgaaatt cgttaaatt tttcgcaga aactctagg accaaatta agttcgtgt ttaagtttt cgtctttat ttattgtta attttaacg ggcaatcta gttactagt tttgttaaa cctaagtgt gtttctttt tattttgaa acgatgttt gttgtgttt gttaaagtg tgaagtgat ttgcataag ttcgaagtg agtgctatg gccgaagga aatggtgaa ttatcagaa gagccaatg aaaattgaa ggggaaccc gagagaacc gaagactat caaaaatta atagaatat ggcctcgat gagaaggtg gctgctaag ttagacgag atttataag actggaaaa ctagcacat gtcgacctc gatgagaga gctctcgat gctctcaag gagtttccc gtcgatggg gcccttaac gttctgggc cagttcctc gactcgaat ttggaacat gtctcgaat aaatcggct tatctttgc ggcgttatg aagacctat cggcagaag agtcgcgcc ggttcgagt caagggacg acaactcct gcacccgct cctgtcaaa ggacccgac gaagagaag atcaagcag attttggac aggacggga tatacgttg gatgtcacc actggtcaa cgcaagtat ggaggtcct ccgccagga tgggatggg tcgcctcca ggtgccggc tgtgaagtc ttctgcggg aagatcccg aaggatatg tacgaggat gaattgatc ccactgttt gagggctgc ggcacgatt tgggatttg agattgatg atggaccca atgaccggc accaacaga gggtatgca ttcgtgacg tttacgaca cgtgatgct gcccagcaa gccgtccaa aagctcgat aatcacgaa atcaaaccc ggcaagacc ctgaaaatt aacattagt gttccgaac ctccgactt ttcgtgggc aacatacca aagtctaaa ggcaaagag gagatactg gaagagttt ggtaaatta acagccggc cttgtggaa gtcatcatc tacagctca ccggacgac aagaagaag aatcgcggc ttctgtttc ctagaatat gaatcgcac aaagccgct tcgttggcc aaacgtcga ctgggcact ggacgaatc aaagtttgg ggttgtgac atcattgtc gattgggct gatccacaa gaagaacca gacgaacaa accatgtcg aaggttaaa gtcttgtac gtacgaaat ttaacgcaa gaaatcagc gaagagaaa ctcaaagaa gcgttcgag gcctatgga aaagtagaa cgtgttaaa aaaattaaa gattacgcc tttatacat ttcgaagat cgggaaaat gccgttaaa gccatggaa gaattagat gggaaagag atgggcggg tccaatata gaagtatcg ttagctaaa cctccgtcc gataaaaag aaaaaggaa gaaatttta agagcgcgt gaacgacgt atgatgcaa atgatgcaa gtgcgtgga ggaatgatg ccaggtgct atgcctctg aggggacca cctggacag ggagcgagg agcggtgcc ggtatgaga ggaccgatg ggccgtggg gattacgat tatgattac gactattac gggtacggg gattatcga ggcggctac agtgaccca tattatgat gactattac cggtatgaa gattactat tatgattat ccgacggcg ccgacgcaa tcccgaggg agaggccgg caaccacaa ccggctggc cgtgggcgt ggggtggtg gcacgtggc cgggccggt gggccccca gcccgtggg gcggcggcc aacagagcg ggccgggga ggagtagca gggggccgt ggggcagca cgccaaacc gctcgtggg ggagtgggc cgcgccaag ggaagttta ccagcaggt aaacggaaa tttgacggg ggtcaccag aaccaaggg gagacaaag cgcagatac cagagcaac tggggtagc cagcccctc ccgcagcag ccgctgggg gcggacgtc aatggagag cagcaggcg tggtaccag gattcgtac gggtcctgg agctaagtg ataggacag tgcagccca cggtgggac tgagtgcga gtggcccta cgtctgggc aagggtggg cgcaccctc tagtgcctg ctggtgtgc gcctgcgcc ctacataga ggaacccaa cagctgatg gtgtcccca tactattaa ttcattgat aatatctat ctgcattcg ttttttttt taaataata atttgttga tttgattac ttcgtatgc catgttgga tttttctaa attttttaa ctgtatcgt ttgaaattc attttgaaa attaaattg tataagtta tttttttta ttatgtttt aatgttatt tgggtgatg tgtcccctt gctctggag gttacatcg cagggacgt cgcccgtcg ttatagtct cggtgcagg ttggcgtcc cgacacatg ggacggaca gtctgcact ttattctaa ttttttctt gtgtttttt tattacttt ttattactt aattgcaac ccgacatcc caggtgtca catcactgc atagattcg gttataatc gtggttttg taaattaaa taaattcgt ctgttggct gttttactg aaacactga ctgtatgga agaagcatg ggtttggca ctgtttagc gcgatcggt ggccgatca ccgatcggg cagggcatg gagggactg cactcggtt catgcagtg tcctgacta agaccgttg ctcatgaaa cgagtgagt tttattata tgtataata taattatat attttattc ctgcaaatg catttttgc attgtgatg tattttagc agtaattta aggagttta aaagcatat acttctgtt tccggcacc ggattaaca attacagtt cagctccag acagaaata ttacagcta aaacactca tttgcaatg ttaggctca atatcatgg ttaatgatt ttttttatt taacaataa ttgtatcga ttaatttta ttgatgatt tgctttttg cttccccgg catatccct ttcagtact acacactgt aacaaagtt gatctgtcc ttttaatct ttagtgtga tgtagctaa tcaattgac attatactg tattagaat tatatacat tctatcact agtaacaca aattgccat tcactacaa ctactccaa ccagctgat tgtgcgtgg tggtgcttg ttcgcaagc ccgccacag ggtgatcca aatcggccg gccgtcgct gactgttac tgtactgta ttaatggtc ggagttgcc agcttcgaa aactcgacg acttgttat gatgtatat cgtctatca attaattct gagaggact gatgattta tatgtctca ataaacagt tgtatgtgt a
- >TC000185 RB
cgactagac ggcttgcaa aacaacgga atctgcaga gtgcacatt gcattacac cgcgggagt cggtatact acatcaaaa cccgaaatt cgttaaatt tttcgcaga aactctagg accaaatta agttcgtgt ttaagtttt cgtctttat ttattgtta attttaacg ggcaatcta gttactagt tttgttaaa cctaagtgt gtttctttt tattttgaa acgatgttt gttgtgttt gttaaagtg tgaagtgat ttgcataag ttcgaagtg agtgctatg gccgaagga aatggtgaa ttatcagaa gagccaatg aaaattgaa ggggaaccc gagagaacc gaagactat caaaaatta atagaatat ggcctcgat gagaaggtg gctgctaag ttagacgag atttataag actggaaaa ctagcacat gtcgacctc gatgagaga gctctcgat gctctcaag gagtttccc gtcgatggg gcccttaac gttctgggc cagttcctc gactcgaat ttggaacat gtctcgaat aaatcggct tatctttgc ggcgttatg aagacctat cggcagaag agtcgcgcc ggttcgagt caagggacg acaactcct gcacccgct cctgtcaaa ggacccgac gaagagaag atcaagcag attttggac aggacggga tatacgttg gatgtcacc actggtcaa cgcaagtat ggaggtcct ccgccagga tgggatggg tcgcctcca ggtgccggc tgtgaagtc ttctgcggg aagatcccg aaggatatg tacgaggat gaattgatc ccactgttt gagggctgc ggcacgatt tgggatttg agattgatg atggaccca atgaccggc accaacaga gggtatgca ttcgtgacg tttacgaca cgtgatgct gcccagcaa gccgtccaa aagctcgat aatcacgaa atcaaaccc ggcaagacc ctgaaaatt aacattagt gttccgaac ctccgactt ttcgtgggc aacatacca aagtctaaa ggcaaagag gagatactg gaagagttt ggtaaatta acagccggc cttgtggaa gtcatcatc tacagctca ccggacgac aagaagaag aatcgcggc ttctgtttc ctagaatat gaatcgcac aaagccgct tcgttggcc aaacgtcga ctgggcact ggacgaatc aaagtttgg ggttgtgac atcattgtc gattgggct gatccacaa gaagaacca gacgaacaa accatgtcg aaggttaaa gtcttgtac gtacgaaat ttaacgcaa gaaatcagc gaagagaaa ctcaaagaa gcgttcgag gcctatgga aaagtagaa cgtgttaaa aaaattaaa gattacgcc tttatacat ttcgaagat cgggaaaat gccgttaaa gccatggaa gaattagat gggaaagag atgggcggg tccaatata gaagtatcg ttagctaaa cctccgtcc gataaaaag aaaaaggaa gaaatttta agagcgcgt gaacgacgt atgatgcaa atgatgcaa gtgcgtgga ggaatgatg ccaggtgct atgcctctg aggggacca cctggacag ggagcgagg agcggtgcc ggtatgaga ggaccgatg ggccgtggg gattacgat tatgattac gactattac gggtacggg gattatcga ggcggctac agtgaccca tattatgat gactattac cggtatgaa gattactat tatgattat ccgacggcg ccgacgcaa tcccgaggg agaggccgg caaccacaa ccggctggc cgtgggcgt ggggtggtg gcacgtggc cgggccggt gggccccca gcccgtggg gcggcggcc aacagagcg ggccgggga ggagtagca gggggccgt ggggcagca cgccaaacc gctcgtggg ggagtgggc cgcgccaag ggaagttta ccaggtaaa cggaaattt gacgggggt caccagaac caaggggag acaaagcgc agataccag agcaactgg ggtagccag cccctcccg cagcagccg ctgggggcg gacgtcaat ggagagcag caggcgtgg taccaggat tcgtacggg tcctggagc taagtgata ggacagtgc agcccacgg tgggactga gtgcgagtg gccctacgt ctgggcaag ggtgggcgc accctctag tgcctgctg gtgtgcgcc tgcgcccta catagagga acccaacag ctgatggtg tccccatac tattaattc attgataat atctatctg cattcgttt tttttttaa ataataatt tgttgattt gattacttc gtatgccat gttggattt ttctaaatt ttttaactg tatcgtttg aaattcatt ttgaaaatt aaattgtat aagttattt tttttatta tgttttaat gttatttgg gtgatgtgt ccccttgct ctggaggtt acatcgcag ggacgtcgc ccgtcgtta tagtctcgg tgcaggttg gcgtcccga cacatggga cggacagtc tgcacttta ttctaattt tttcttgtg tttttttat tacttttta ttacttaat tgcaacccg acatcccag gtgtcacat cactgcata gattcggtt ataatcgtg gttttgtaa attaaataa attcgtctg ttggctgtt ttactgaaa cactgactg tatggaaga agcatgggt ttggcactg tttagcgcg atcggtggc cgatcaccg atcgggcag ggcatggag ggactgcac tcggttcat gcagtgtcc tgactaaga ccgttgctc atgaaacga gtgagtttt attatatgt ataatataa ttatatatt ttattcctg caaatgcat ttttgcatt gtgatgtat tttagcagt aatttaagg agtttaaaa gcatatact tctgtttcc ggcaccgga ttaacaatt acagttcag ctccagaca gaaatatta cagctaaaa cactcattt gcaatgtta ggctcaata tcatggtta atgattttt tttatttaa caataattg tatcgatta attttattg atgatttgc tttttgctt ccccggcat atccctttc agtactaca cactgtaac aaagttgat ctgtccttt taatcttta gtgtgatgt agctaatca attgacatt atactgtat tagaattat atacattct atcactagt aacacaaat tgccattca ctacaacta ctccaacca gctgattgt gcgtggtgg tgcttgttc gcaagcccg ccacagggt gatccaaat cggccggcc gtcgctgac tgttactgt actgtatta atggtcgga gttgccagc ttcgaaaac tcgacgact tgttatgat gtatatcgt ctatcaatt aattctgag aggactgat gatttatat gtctcaata aacagttgt atgtgta
