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Gene information
General information
TC000185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14993998..15005729
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000185
    iBeetle screen iB_00037

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection



    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000185 RA
      MAEGNGELS EEPMKIEGE PERTEDYQK LIEYGLDEK 
      VAAKLDEIY KTGKLAHVD LDERALDAL KEFPVDGAL 
      NVLGQFLDS NLEHVSNKS AYLCGVMKT YRQKSRAGS 
      SQGTTTPAP APVKGPDEE KIKQILDRT GYTLDVTTG 
      QRKYGGPPP GWDGSPPGA GCEVFCGKI PKDMYEDEL 
      IPLFEGCGT IWDLRLMMD PMTGTNRGY AFVTFTTRD 
      AAQQAVQKL DNHEIKPGK TLKINISVP NLRLFVGNI 
      PKSKGKEEI LEEFGKLTA GLVEVIIYS SPDDKKKNR 
      GFCFLEYES HKAASLAKR RLGTGRIKV WGCDIIVDW 
      ADPQEEPDE QTMSKVKVL YVRNLTQEI SEEKLKEAF 
      EAYGKVERV KKIKDYAFI HFEDRENAV KAMEELDGK 
      EMGGSNIEV SLAKPPSDK KKKEEILRA RERRMMQMM 
      QVRGGMMPG AMPLRGPPG QGARSGAGM RGPMGRGDY 
      DYDYDYYGY GDYRGGYSD PYYDDYYRY EDYYYDYPT 
      APTQSRGRG RQPQPAGRG RGVVARGRA GGPPARGAA 
      ANRAGRGGV AGGRGAARQ TARGGVGRA KGSLPAGKR 
      KFDGGHQNQ GETKRRYQS NWGSQPLPQ QPLGADVNG 
      EQQAWYQDS YGSWS
    • >TC000185 RB
      MAEGNGELS EEPMKIEGE PERTEDYQK LIEYGLDEK 
      VAAKLDEIY KTGKLAHVD LDERALDAL KEFPVDGAL 
      NVLGQFLDS NLEHVSNKS AYLCGVMKT YRQKSRAGS 
      SQGTTTPAP APVKGPDEE KIKQILDRT GYTLDVTTG 
      QRKYGGPPP GWDGSPPGA GCEVFCGKI PKDMYEDEL 
      IPLFEGCGT IWDLRLMMD PMTGTNRGY AFVTFTTRD 
      AAQQAVQKL DNHEIKPGK TLKINISVP NLRLFVGNI 
      PKSKGKEEI LEEFGKLTA GLVEVIIYS SPDDKKKNR 
      GFCFLEYES HKAASLAKR RLGTGRIKV WGCDIIVDW 
      ADPQEEPDE QTMSKVKVL YVRNLTQEI SEEKLKEAF 
      EAYGKVERV KKIKDYAFI HFEDRENAV KAMEELDGK 
      EMGGSNIEV SLAKPPSDK KKKEEILRA RERRMMQMM 
      QVRGGMMPG AMPLRGPPG QGARSGAGM RGPMGRGDY 
      DYDYDYYGY GDYRGGYSD PYYDDYYRY EDYYYDYPT 
      APTQSRGRG RQPQPAGRG RGVVARGRA GGPPARGAA 
      ANRAGRGGV AGGRGAARQ TARGGVGRA KGSLPGKRK 
      FDGGHQNQG ETKRRYQSN WGSQPLPQQ PLGADVNGE 
      QQAWYQDSY GSWS
    CDS sequence
    • >TC000185 RA
      atggccgaa ggaaatggt gaattatca gaagagcca 
      atgaaaatt gaaggggaa cccgagaga accgaagac 
      tatcaaaaa ttaatagaa tatggcctc gatgagaag 
      gtggctgct aagttagac gagatttat aagactgga 
      aaactagca catgtcgac ctcgatgag agagctctc 
      gatgctctc aaggagttt cccgtcgat ggggccctt 
      aacgttctg ggccagttc ctcgactcg aatttggaa 
      catgtctcg aataaatcg gcttatctt tgcggcgtt 
      atgaagacc tatcggcag aagagtcgc gccggttcg 
      agtcaaggg acgacaact cctgcaccc gctcctgtc 
      aaaggaccc gacgaagag aagatcaag cagattttg 
      gacaggacg ggatatacg ttggatgtc accactggt 
      caacgcaag tatggaggt cctccgcca ggatgggat 
      gggtcgcct ccaggtgcc ggctgtgaa gtcttctgc 
      gggaagatc ccgaaggat atgtacgag gatgaattg 
      atcccactg tttgagggc tgcggcacg atttgggat 
      ttgagattg atgatggac ccaatgacc ggcaccaac 
      agagggtat gcattcgtg acgtttacg acacgtgat 
      gctgcccag caagccgtc caaaagctc gataatcac 
      gaaatcaaa cccggcaag accctgaaa attaacatt 
      agtgttccg aacctccga cttttcgtg ggcaacata 
      ccaaagtct aaaggcaaa gaggagata ctggaagag 
      tttggtaaa ttaacagcc ggccttgtg gaagtcatc 
      atctacagc tcaccggac gacaagaag aagaatcgc 
      ggcttctgt ttcctagaa tatgaatcg cacaaagcc 
      gcttcgttg gccaaacgt cgactgggc actggacga 
      atcaaagtt tggggttgt gacatcatt gtcgattgg 
      gctgatcca caagaagaa ccagacgaa caaaccatg 
      tcgaaggtt aaagtcttg tacgtacga aatttaacg 
      caagaaatc agcgaagag aaactcaaa gaagcgttc 
      gaggcctat ggaaaagta gaacgtgtt aaaaaaatt 
      aaagattac gcctttata catttcgaa gatcgggaa 
      aatgccgtt aaagccatg gaagaatta gatgggaaa 
      gagatgggc gggtccaat atagaagta tcgttagct 
      aaacctccg tccgataaa aagaaaaag gaagaaatt 
      ttaagagcg cgtgaacga cgtatgatg caaatgatg 
      caagtgcgt ggaggaatg atgccaggt gctatgcct 
      ctgagggga ccacctgga cagggagcg aggagcggt 
      gccggtatg agaggaccg atgggccgt ggggattac 
      gattatgat tacgactat tacgggtac ggggattat 
      cgaggcggc tacagtgac ccatattat gatgactat 
      taccggtat gaagattac tattatgat tatccgacg 
      gcgccgacg caatcccga gggagaggc cggcaacca 
      caaccggct ggccgtggg cgtggggtg gtggcacgt 
      ggccgggcc ggtgggccc ccagcccgt ggggcggcg 
      gccaacaga gcgggccgg ggaggagta gcagggggc 
      cgtggggca gcacgccaa accgctcgt gggggagtg 
      ggccgcgcc aagggaagt ttaccagca ggtaaacgg 
      aaatttgac gggggtcac cagaaccaa ggggagaca 
      aagcgcaga taccagagc aactggggt agccagccc 
      ctcccgcag cagccgctg ggggcggac gtcaatgga 
      gagcagcag gcgtggtac caggattcg tacgggtcc 
      tggagctaa
    • >TC000185 RB
      atggccgaa ggaaatggt gaattatca gaagagcca 
      atgaaaatt gaaggggaa cccgagaga accgaagac 
      tatcaaaaa ttaatagaa tatggcctc gatgagaag 
      gtggctgct aagttagac gagatttat aagactgga 
      aaactagca catgtcgac ctcgatgag agagctctc 
      gatgctctc aaggagttt cccgtcgat ggggccctt 
      aacgttctg ggccagttc ctcgactcg aatttggaa 
      catgtctcg aataaatcg gcttatctt tgcggcgtt 
      atgaagacc tatcggcag aagagtcgc gccggttcg 
      agtcaaggg acgacaact cctgcaccc gctcctgtc 
      aaaggaccc gacgaagag aagatcaag cagattttg 
      gacaggacg ggatatacg ttggatgtc accactggt 
      caacgcaag tatggaggt cctccgcca ggatgggat 
      gggtcgcct ccaggtgcc ggctgtgaa gtcttctgc 
      gggaagatc ccgaaggat atgtacgag gatgaattg 
      atcccactg tttgagggc tgcggcacg atttgggat 
      ttgagattg atgatggac ccaatgacc ggcaccaac 
      agagggtat gcattcgtg acgtttacg acacgtgat 
      gctgcccag caagccgtc caaaagctc gataatcac 
      gaaatcaaa cccggcaag accctgaaa attaacatt 
      agtgttccg aacctccga cttttcgtg ggcaacata 
      ccaaagtct aaaggcaaa gaggagata ctggaagag 
      tttggtaaa ttaacagcc ggccttgtg gaagtcatc 
      atctacagc tcaccggac gacaagaag aagaatcgc 
      ggcttctgt ttcctagaa tatgaatcg cacaaagcc 
      gcttcgttg gccaaacgt cgactgggc actggacga 
      atcaaagtt tggggttgt gacatcatt gtcgattgg 
      gctgatcca caagaagaa ccagacgaa caaaccatg 
      tcgaaggtt aaagtcttg tacgtacga aatttaacg 
      caagaaatc agcgaagag aaactcaaa gaagcgttc 
      gaggcctat ggaaaagta gaacgtgtt aaaaaaatt 
      aaagattac gcctttata catttcgaa gatcgggaa 
      aatgccgtt aaagccatg gaagaatta gatgggaaa 
      gagatgggc gggtccaat atagaagta tcgttagct 
      aaacctccg tccgataaa aagaaaaag gaagaaatt 
      ttaagagcg cgtgaacga cgtatgatg caaatgatg 
      caagtgcgt ggaggaatg atgccaggt gctatgcct 
      ctgagggga ccacctgga cagggagcg aggagcggt 
      gccggtatg agaggaccg atgggccgt ggggattac 
      gattatgat tacgactat tacgggtac ggggattat 
      cgaggcggc tacagtgac ccatattat gatgactat 
      taccggtat gaagattac tattatgat tatccgacg 
      gcgccgacg caatcccga gggagaggc cggcaacca 
      caaccggct ggccgtggg cgtggggtg gtggcacgt 
      ggccgggcc ggtgggccc ccagcccgt ggggcggcg 
      gccaacaga gcgggccgg ggaggagta gcagggggc 
      cgtggggca gcacgccaa accgctcgt gggggagtg 
      ggccgcgcc aagggaagt ttaccaggt aaacggaaa 
      tttgacggg ggtcaccag aaccaaggg gagacaaag 
      cgcagatac cagagcaac tggggtagc cagcccctc 
      ccgcagcag ccgctgggg gcggacgtc aatggagag 
      cagcaggcg tggtaccag gattcgtac gggtcctgg 
      agctaa
    mRNA sequence
    • >TC000185 RA
      cgactagac ggcttgcaa aacaacgga atctgcaga 
      gtgcacatt gcattacac cgcgggagt cggtatact 
      acatcaaaa cccgaaatt cgttaaatt tttcgcaga 
      aactctagg accaaatta agttcgtgt ttaagtttt 
      cgtctttat ttattgtta attttaacg ggcaatcta 
      gttactagt tttgttaaa cctaagtgt gtttctttt 
      tattttgaa acgatgttt gttgtgttt gttaaagtg 
      tgaagtgat ttgcataag ttcgaagtg agtgctatg 
      gccgaagga aatggtgaa ttatcagaa gagccaatg 
      aaaattgaa ggggaaccc gagagaacc gaagactat 
      caaaaatta atagaatat ggcctcgat gagaaggtg 
      gctgctaag ttagacgag atttataag actggaaaa 
      ctagcacat gtcgacctc gatgagaga gctctcgat 
      gctctcaag gagtttccc gtcgatggg gcccttaac 
      gttctgggc cagttcctc gactcgaat ttggaacat 
      gtctcgaat aaatcggct tatctttgc ggcgttatg 
      aagacctat cggcagaag agtcgcgcc ggttcgagt 
      caagggacg acaactcct gcacccgct cctgtcaaa 
      ggacccgac gaagagaag atcaagcag attttggac 
      aggacggga tatacgttg gatgtcacc actggtcaa 
      cgcaagtat ggaggtcct ccgccagga tgggatggg 
      tcgcctcca ggtgccggc tgtgaagtc ttctgcggg 
      aagatcccg aaggatatg tacgaggat gaattgatc 
      ccactgttt gagggctgc ggcacgatt tgggatttg 
      agattgatg atggaccca atgaccggc accaacaga 
      gggtatgca ttcgtgacg tttacgaca cgtgatgct 
      gcccagcaa gccgtccaa aagctcgat aatcacgaa 
      atcaaaccc ggcaagacc ctgaaaatt aacattagt 
      gttccgaac ctccgactt ttcgtgggc aacatacca 
      aagtctaaa ggcaaagag gagatactg gaagagttt 
      ggtaaatta acagccggc cttgtggaa gtcatcatc 
      tacagctca ccggacgac aagaagaag aatcgcggc 
      ttctgtttc ctagaatat gaatcgcac aaagccgct 
      tcgttggcc aaacgtcga ctgggcact ggacgaatc 
      aaagtttgg ggttgtgac atcattgtc gattgggct 
      gatccacaa gaagaacca gacgaacaa accatgtcg 
      aaggttaaa gtcttgtac gtacgaaat ttaacgcaa 
      gaaatcagc gaagagaaa ctcaaagaa gcgttcgag 
      gcctatgga aaagtagaa cgtgttaaa aaaattaaa 
      gattacgcc tttatacat ttcgaagat cgggaaaat 
      gccgttaaa gccatggaa gaattagat gggaaagag 
      atgggcggg tccaatata gaagtatcg ttagctaaa 
      cctccgtcc gataaaaag aaaaaggaa gaaatttta 
      agagcgcgt gaacgacgt atgatgcaa atgatgcaa 
      gtgcgtgga ggaatgatg ccaggtgct atgcctctg 
      aggggacca cctggacag ggagcgagg agcggtgcc 
      ggtatgaga ggaccgatg ggccgtggg gattacgat 
      tatgattac gactattac gggtacggg gattatcga 
      ggcggctac agtgaccca tattatgat gactattac 
      cggtatgaa gattactat tatgattat ccgacggcg 
      ccgacgcaa tcccgaggg agaggccgg caaccacaa 
      ccggctggc cgtgggcgt ggggtggtg gcacgtggc 
      cgggccggt gggccccca gcccgtggg gcggcggcc 
      aacagagcg ggccgggga ggagtagca gggggccgt 
      ggggcagca cgccaaacc gctcgtggg ggagtgggc 
      cgcgccaag ggaagttta ccagcaggt aaacggaaa 
      tttgacggg ggtcaccag aaccaaggg gagacaaag 
      cgcagatac cagagcaac tggggtagc cagcccctc 
      ccgcagcag ccgctgggg gcggacgtc aatggagag 
      cagcaggcg tggtaccag gattcgtac gggtcctgg 
      agctaagtg ataggacag tgcagccca cggtgggac 
      tgagtgcga gtggcccta cgtctgggc aagggtggg 
      cgcaccctc tagtgcctg ctggtgtgc gcctgcgcc 
      ctacataga ggaacccaa cagctgatg gtgtcccca 
      tactattaa ttcattgat aatatctat ctgcattcg 
      ttttttttt taaataata atttgttga tttgattac 
      ttcgtatgc catgttgga tttttctaa attttttaa 
      ctgtatcgt ttgaaattc attttgaaa attaaattg 
      tataagtta tttttttta ttatgtttt aatgttatt 
      tgggtgatg tgtcccctt gctctggag gttacatcg 
      cagggacgt cgcccgtcg ttatagtct cggtgcagg 
      ttggcgtcc cgacacatg ggacggaca gtctgcact 
      ttattctaa ttttttctt gtgtttttt tattacttt 
      ttattactt aattgcaac ccgacatcc caggtgtca 
      catcactgc atagattcg gttataatc gtggttttg 
      taaattaaa taaattcgt ctgttggct gttttactg 
      aaacactga ctgtatgga agaagcatg ggtttggca 
      ctgtttagc gcgatcggt ggccgatca ccgatcggg 
      cagggcatg gagggactg cactcggtt catgcagtg 
      tcctgacta agaccgttg ctcatgaaa cgagtgagt 
      tttattata tgtataata taattatat attttattc 
      ctgcaaatg catttttgc attgtgatg tattttagc 
      agtaattta aggagttta aaagcatat acttctgtt 
      tccggcacc ggattaaca attacagtt cagctccag 
      acagaaata ttacagcta aaacactca tttgcaatg 
      ttaggctca atatcatgg ttaatgatt ttttttatt 
      taacaataa ttgtatcga ttaatttta ttgatgatt 
      tgctttttg cttccccgg catatccct ttcagtact 
      acacactgt aacaaagtt gatctgtcc ttttaatct 
      ttagtgtga tgtagctaa tcaattgac attatactg 
      tattagaat tatatacat tctatcact agtaacaca 
      aattgccat tcactacaa ctactccaa ccagctgat 
      tgtgcgtgg tggtgcttg ttcgcaagc ccgccacag 
      ggtgatcca aatcggccg gccgtcgct gactgttac 
      tgtactgta ttaatggtc ggagttgcc agcttcgaa 
      aactcgacg acttgttat gatgtatat cgtctatca 
      attaattct gagaggact gatgattta tatgtctca 
      ataaacagt tgtatgtgt a
    • >TC000185 RB
      cgactagac ggcttgcaa aacaacgga atctgcaga 
      gtgcacatt gcattacac cgcgggagt cggtatact 
      acatcaaaa cccgaaatt cgttaaatt tttcgcaga 
      aactctagg accaaatta agttcgtgt ttaagtttt 
      cgtctttat ttattgtta attttaacg ggcaatcta 
      gttactagt tttgttaaa cctaagtgt gtttctttt 
      tattttgaa acgatgttt gttgtgttt gttaaagtg 
      tgaagtgat ttgcataag ttcgaagtg agtgctatg 
      gccgaagga aatggtgaa ttatcagaa gagccaatg 
      aaaattgaa ggggaaccc gagagaacc gaagactat 
      caaaaatta atagaatat ggcctcgat gagaaggtg 
      gctgctaag ttagacgag atttataag actggaaaa 
      ctagcacat gtcgacctc gatgagaga gctctcgat 
      gctctcaag gagtttccc gtcgatggg gcccttaac 
      gttctgggc cagttcctc gactcgaat ttggaacat 
      gtctcgaat aaatcggct tatctttgc ggcgttatg 
      aagacctat cggcagaag agtcgcgcc ggttcgagt 
      caagggacg acaactcct gcacccgct cctgtcaaa 
      ggacccgac gaagagaag atcaagcag attttggac 
      aggacggga tatacgttg gatgtcacc actggtcaa 
      cgcaagtat ggaggtcct ccgccagga tgggatggg 
      tcgcctcca ggtgccggc tgtgaagtc ttctgcggg 
      aagatcccg aaggatatg tacgaggat gaattgatc 
      ccactgttt gagggctgc ggcacgatt tgggatttg 
      agattgatg atggaccca atgaccggc accaacaga 
      gggtatgca ttcgtgacg tttacgaca cgtgatgct 
      gcccagcaa gccgtccaa aagctcgat aatcacgaa 
      atcaaaccc ggcaagacc ctgaaaatt aacattagt 
      gttccgaac ctccgactt ttcgtgggc aacatacca 
      aagtctaaa ggcaaagag gagatactg gaagagttt 
      ggtaaatta acagccggc cttgtggaa gtcatcatc 
      tacagctca ccggacgac aagaagaag aatcgcggc 
      ttctgtttc ctagaatat gaatcgcac aaagccgct 
      tcgttggcc aaacgtcga ctgggcact ggacgaatc 
      aaagtttgg ggttgtgac atcattgtc gattgggct 
      gatccacaa gaagaacca gacgaacaa accatgtcg 
      aaggttaaa gtcttgtac gtacgaaat ttaacgcaa 
      gaaatcagc gaagagaaa ctcaaagaa gcgttcgag 
      gcctatgga aaagtagaa cgtgttaaa aaaattaaa 
      gattacgcc tttatacat ttcgaagat cgggaaaat 
      gccgttaaa gccatggaa gaattagat gggaaagag 
      atgggcggg tccaatata gaagtatcg ttagctaaa 
      cctccgtcc gataaaaag aaaaaggaa gaaatttta 
      agagcgcgt gaacgacgt atgatgcaa atgatgcaa 
      gtgcgtgga ggaatgatg ccaggtgct atgcctctg 
      aggggacca cctggacag ggagcgagg agcggtgcc 
      ggtatgaga ggaccgatg ggccgtggg gattacgat 
      tatgattac gactattac gggtacggg gattatcga 
      ggcggctac agtgaccca tattatgat gactattac 
      cggtatgaa gattactat tatgattat ccgacggcg 
      ccgacgcaa tcccgaggg agaggccgg caaccacaa 
      ccggctggc cgtgggcgt ggggtggtg gcacgtggc 
      cgggccggt gggccccca gcccgtggg gcggcggcc 
      aacagagcg ggccgggga ggagtagca gggggccgt 
      ggggcagca cgccaaacc gctcgtggg ggagtgggc 
      cgcgccaag ggaagttta ccaggtaaa cggaaattt 
      gacgggggt caccagaac caaggggag acaaagcgc 
      agataccag agcaactgg ggtagccag cccctcccg 
      cagcagccg ctgggggcg gacgtcaat ggagagcag 
      caggcgtgg taccaggat tcgtacggg tcctggagc 
      taagtgata ggacagtgc agcccacgg tgggactga 
      gtgcgagtg gccctacgt ctgggcaag ggtgggcgc 
      accctctag tgcctgctg gtgtgcgcc tgcgcccta 
      catagagga acccaacag ctgatggtg tccccatac 
      tattaattc attgataat atctatctg cattcgttt 
      tttttttaa ataataatt tgttgattt gattacttc 
      gtatgccat gttggattt ttctaaatt ttttaactg 
      tatcgtttg aaattcatt ttgaaaatt aaattgtat 
      aagttattt tttttatta tgttttaat gttatttgg 
      gtgatgtgt ccccttgct ctggaggtt acatcgcag 
      ggacgtcgc ccgtcgtta tagtctcgg tgcaggttg 
      gcgtcccga cacatggga cggacagtc tgcacttta 
      ttctaattt tttcttgtg tttttttat tacttttta 
      ttacttaat tgcaacccg acatcccag gtgtcacat 
      cactgcata gattcggtt ataatcgtg gttttgtaa 
      attaaataa attcgtctg ttggctgtt ttactgaaa 
      cactgactg tatggaaga agcatgggt ttggcactg 
      tttagcgcg atcggtggc cgatcaccg atcgggcag 
      ggcatggag ggactgcac tcggttcat gcagtgtcc 
      tgactaaga ccgttgctc atgaaacga gtgagtttt 
      attatatgt ataatataa ttatatatt ttattcctg 
      caaatgcat ttttgcatt gtgatgtat tttagcagt 
      aatttaagg agtttaaaa gcatatact tctgtttcc 
      ggcaccgga ttaacaatt acagttcag ctccagaca 
      gaaatatta cagctaaaa cactcattt gcaatgtta 
      ggctcaata tcatggtta atgattttt tttatttaa 
      caataattg tatcgatta attttattg atgatttgc 
      tttttgctt ccccggcat atccctttc agtactaca 
      cactgtaac aaagttgat ctgtccttt taatcttta 
      gtgtgatgt agctaatca attgacatt atactgtat 
      tagaattat atacattct atcactagt aacacaaat 
      tgccattca ctacaacta ctccaacca gctgattgt 
      gcgtggtgg tgcttgttc gcaagcccg ccacagggt 
      gatccaaat cggccggcc gtcgctgac tgttactgt 
      actgtatta atggtcgga gttgccagc ttcgaaaac 
      tcgacgact tgttatgat gtatatcgt ctatcaatt 
      aattctgag aggactgat gatttatat gtctcaata 
      aacagttgt atgtgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY