Status
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Gene information
General information
TC000182 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15023993..15029209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000182
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00036

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000182 RA
      MMAATLTPE QEEATKDFI EKVNAKYRR KLGPVSWST 
      AVRFLYARK FDVLRALVL FEQHELTRQ REGLTCFDP 
      LKEPLQSEL LTGKFTILP KRDASDAAI AVFTAHKHS 
      PASTTHQVT LQGVVYQLD VALQNPVTQ KAGIVFIYD 
      MSNSKYSNF DYDLSQKIL TLLKGGYPA KLKKVLIVT 
      APLWFKAPF KILRLFVRE KLRERVYTV SVAQLLTHI 
      PRESLPTQL GGSLILDHN GWLSLCLQS MTTREETSL 
      PQYDGKTPS PQKRIRTPT EIEISSSTN NINDTWAEE 
      GNLNPPSSA SSGFSDDDS LHGDGAALT MPQLVDYVR 
      DRGRSGLMQ EYAEIRSRP PDGTFNVAK MKNNLPKNR 
      YTDVLCYDH SRVILSEID CDKDSDYIH ANFVDGYKQ 
      KNAFINTQG PLPKTTPDF WRMIWEQHS LVIVMTTRV 
      MERGRPKCH QYWETEVGS EASYGQFTV KTVAVETDP 
      DYTVTTINL INNKTDESR EVSHWQFTS WPDYGVPRS 
      AKAMLEFLE RVRRKQNAM VVALGDTWA GHPRGPPIV 
      VHCSAGIGR TGTFCTLDI CISRLEDLG TADIRGTVE 
      RIRSQRAYS IQMPDQYIF CHLALIEYA LMKGHLQSA 
      DLAGFDPGA DDDSD
    CDS sequence
    • >TC000182 RA
      atgatggct gctacatta accccagaa caagaggaa 
      gcaacgaaa gatttcatc gaaaaagtc aacgctaaa 
      tatcgtcga aaattgggc cctgtgtca tggtcaaca 
      gccgttcgg tttctctac gctcgaaaa ttcgacgtt 
      cttcgagct ttggtcctt ttcgaacag cacgaattg 
      acacgacaa cgtgaaggc ctaacgtgt ttcgatccg 
      ttaaaggaa ccactacag tctgaatta cttacaggg 
      aaatttacg attttgccg aaacgagat gcctccgat 
      gctgcgata gctgtgttc actgctcat aaacactcc 
      ccggctagt acaacccat caagtcact ctacagggt 
      gttgtttat caacttgat gtggcgctg caaaatccc 
      gtcacacaa aaagccgga attgtgttt atttacgac 
      atgtccaat tcgaaatat tcaaatttc gattatgat 
      ctgtcgcaa aagattctc actttattg aagggcggc 
      tatccggcc aaacttaaa aaggtgctg atcgtgacg 
      gcccccttg tggttcaaa gcccccttc aaaatcctg 
      cgtcttttc gtccgtgaa aaactccgt gagagagtt 
      tacaccgtt tcagtggcc caactttta acccatata 
      ccacgagaa tctctacca acacagttg ggcggctca 
      ctcatcctg gaccataat ggttggcta tcgttgtgt 
      ttgcagtca atgaccact cgagaggaa acctcattg 
      ccacaatat gatggcaaa acgccatca ccccaaaaa 
      cgcattcgc acaccaacc gaaatcgaa atctcgtca 
      tcgaccaat aacataaac gacacctgg gccgaagaa 
      ggcaatttg aatccgcca agtagcgca tcgagtggt 
      ttctctgac gatgattct ttgcatgga gatggggct 
      gcacttaca atgccccaa ttggtggat tatgtgcga 
      gataggggg cgttcgggg ctcatgcag gagtacgca 
      gagattcgg tcaaggccc cctgatggc accttcaac 
      gtcgccaaa atgaaaaac aacttgccg aaaaatcga 
      tacacggat gtgttgtgt tacgatcat agtcgagtg 
      attctctcc gaaatcgat tgtgataaa gacagtgat 
      tatatacat gcgaatttt gtcgatggg tataagcag 
      aaaaacgcg tttattaac acacaaggt cctttaccg 
      aaaacaacg cccgatttt tggcgaatg atttgggaa 
      caacatagt ttagtgata gtgatgacg actcgagtg 
      atggaacga gggcgtccc aagtgtcac cagtactgg 
      gagactgaa gttgggagt gaagcgagt tatggtcaa 
      ttcactgtt aaaactgta gctgttgaa accgatccc 
      gactacacc gtcacaact attaatctt ataaataat 
      aaaaccgac gaatctagg gaagtttcg cattggcag 
      tttacgtca tggcccgat tatggggtt ccccgttcg 
      gctaaagcc atgctcgag tttctcgaa cgtgtgcgc 
      cgaaaacag aacgctatg gtggtagca ctgggtgat 
      acatgggcg gggcatccc cgaggaccg ccgattgtg 
      gtccattgt agtgcagga attggacga acggggact 
      ttttgtacc ttggatatt tgtatatcc cgactggag 
      gatttagga acggcagat attcgggga actgtcgaa 
      cgaatccga tcacaacgt gcatattcc atccaaatg 
      cctgatcag tacattttt tgtcacctc gctttgatc 
      gaatacgcc ctaatgaag ggacactta cagtcggct 
      gatttggcc ggattcgat ccaggcgct gacgacgat 
      tcggactaa
    mRNA sequence
    • >TC000182 RA
      ccacccgtc tgtcaaatt ttgttgtgt tgtgtgcgt 
      gtacaatga tggctgcta cattaaccc cagaacaag 
      aggaagcaa cgaaagatt tcatcgaaa aagtcaacg 
      ctaaatatc gtcgaaaat tgggccctg tgtcatggt 
      caacagccg ttcggtttc tctacgctc gaaaattcg 
      acgttcttc gagctttgg tccttttcg aacagcacg 
      aattgacac gacaacgtg aaggcctaa cgtgtttcg 
      atccgttaa aggaaccac tacagtctg aattactta 
      cagggaaat ttacgattt tgccgaaac gagatgcct 
      ccgatgctg cgatagctg tgttcactg ctcataaac 
      actccccgg ctagtacaa cccatcaag tcactctac 
      agggtgttg tttatcaac ttgatgtgg cgctgcaaa 
      atcccgtca cacaaaaag ccggaattg tgtttattt 
      acgacatgt ccaattcga aatattcaa atttcgatt 
      atgatctgt cgcaaaaga ttctcactt tattgaagg 
      gcggctatc cggccaaac ttaaaaagg tgctgatcg 
      tgacggccc ccttgtggt tcaaagccc ccttcaaaa 
      tcctgcgtc ttttcgtcc gtgaaaaac tccgtgaga 
      gagtttaca ccgtttcag tggcccaac ttttaaccc 
      atataccac gagaatctc taccaacac agttgggcg 
      gctcactca tcctggacc ataatggtt ggctatcgt 
      tgtgtttgc agtcaatga ccactcgag aggaaacct 
      cattgccac aatatgatg gcaaaacgc catcacccc 
      aaaaacgca ttcgcacac caaccgaaa tcgaaatct 
      cgtcatcga ccaataaca taaacgaca cctgggccg 
      aagaaggca atttgaatc cgccaagta gcgcatcga 
      gtggtttct ctgacgatg attctttgc atggagatg 
      gggctgcac ttacaatgc cccaattgg tggattatg 
      tgcgagata gggggcgtt cggggctca tgcaggagt 
      acgcagaga ttcggtcaa ggccccctg atggcacct 
      tcaacgtcg ccaaaatga aaaacaact tgccgaaaa 
      atcgataca cggatgtgt tgtgttacg atcatagtc 
      gagtgattc tctccgaaa tcgattgtg ataaagaca 
      gtgattata tacatgcga attttgtcg atgggtata 
      agcagaaaa acgcgttta ttaacacac aaggtcctt 
      taccgaaaa caacgcccg atttttggc gaatgattt 
      gggaacaac atagtttag tgatagtga tgacgactc 
      gagtgatgg aacgagggc gtcccaagt gtcaccagt 
      actgggaga ctgaagttg ggagtgaag cgagttatg 
      gtcaattca ctgttaaaa ctgtagctg ttgaaaccg 
      atcccgact acaccgtca caactatta atcttataa 
      ataataaaa ccgacgaat ctagggaag tttcgcatt 
      ggcagttta cgtcatggc ccgattatg gggttcccc 
      gttcggcta aagccatgc tcgagtttc tcgaacgtg 
      tgcgccgaa aacagaacg ctatggtgg tagcactgg 
      gtgatacat gggcggggc atccccgag gaccgccga 
      ttgtggtcc attgtagtg caggaattg gacgaacgg 
      ggacttttt gtaccttgg atatttgta tatcccgac 
      tggaggatt taggaacgg cagatattc ggggaactg 
      tcgaacgaa tccgatcac aacgtgcat attccatcc 
      aaatgcctg atcagtaca ttttttgtc acctcgctt 
      tgatcgaat acgccctaa tgaagggac acttacagt 
      cggctgatt tggccggat tcgatccag gcgctgacg 
      acgattcgg actaatttt aattctgct tcaaacttt 
      ttgtcttgt gaacgacaa attgtgatt cttttctaa 
      atttacata tcgttttct caaataaaa ccgatcttg 
      taatatgac agcgaaaga tggcgttgc taatgtgac 
      aggtcactt tgattgcga tggaatatt acaagttta 
      tttttataa tcctaattc aatgttgga tgtacctac 
      atttttaag cttccttga atatttgaa actaggcta 
      atgaaatca agaacgatc ttgttcttt acatataaa 
      gactgtcca ataaaatgt aaaattatt tattttgca 
      aagctttag gaacgcccc gatttgtgt tttaccaaa 
      aacaacttc cattcaaga ggttcaagc caaaacgtt 
      agtgtttaa caatttttt ttgtactaa gtattattg 
      tgacatagt tgaattgta ttgtaaata ttgtattct 
      gatttctta atacaacaa aaaatgctt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY