| General information | |||
| TC000175 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 15083452..15090509 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000175 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00034 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC000175 RA
MWYFLALLH LTLAEPKGS GYSIFLEHT PSWDRRPYG YGPAAPVAF DPFNNNNFL PQPQPQPPT PDPATLGCG TPPAGCPKS RYRSYDGSC NNLGNPILG TPQTRYARL LPANYADGV STPTASVTG KPLPGSRIV SLLIYPDVQ IEDPKFTIN AMQYGQIIT HDMSMIAGS TQAQPHQTK CCTADGQLL EFANIPEHC FPIVLPNDD PAHSQTNAK CMNFVRTIT DRDRNCVGG SQPAEQLTA VNHYLDLSI VYGNSDQIN QQVRQFQGG RLRVEVRDG KEWLPRSTN ASGVCSIQS PQEVCYLAG DARVNQNPQ LTILQIILM REHNRIADA LAKLNPHWD DETIFQEAR RIAIAEHQF ISYYEWLPI FIGIENSLK NKIIYLSKH FINDYRQEV DPTVLNEHA TAAFRYFHS LIAGHLDLV NEHRSSYGN LRLSDWFNR PAIVEQGDN FDELTRGLA TQPELASDP YHDSEITQF LFRDGQQFG SDLKAIDIQ RNRDHGLAS YNEYRGFCG LPRAHSFED FLDVMTQDN VKKLATLYE SPDDVDLTV GGSLEAHVP GTLSGPTFL CILTEQFYR TRVGDRFWF ENQHNGFTL AQLNAIRKT SISRLLCDN GHHIQAMQP RGFERISHD NPITPCENL PAVDLTLWK EVPFGVERP YDFGSDFSD EFFWKKK
- >TC000175 RA
atgtggtac ttcctggcc cttctccac ttgacctta gctgaaccc aaaggctcg ggttatagc attttcctc gaacacacg ccaagttgg gacaggaga ccttacggt tacggacct gcggcacca gtggccttt gaccctttc aacaataac aatttcttg ccacaacca caaccacaa cccccaaca cccgatccg gcaacctta ggctgtggt accccacca gcagggtgc cccaaatcg aggtaccgc tcctacgat ggttcctgt aataactta gggaaccca attttgggt actccacag acacgatac gcgaggctt ctaccggcg aattacgcc gatggggtg agcaccccc acagcttca gtaacggga aaaccgctc ccaggctct cgaatagtc agtcttttg atttatccc gatgtacaa attgaagat cccaaattt acaattaat gccatgcaa tatgggcaa ataatcacc catgatatg agcatgatt gcgggaagt acccaagca cagccccat cagaccaag tgttgtacg gccgatggt caattgctc gagtttgcc aacataccg gaacattgt ttccctata gtgttgccc aacgatgac cccgctcac tcccagacg aatgcaaag tgcatgaat tttgtaaga acgatcact gatcgtgat cgcaactgt gttggaggc tcacaaccg gctgaacaa ttaactgca gttaaccat tacttggac ttatcgata gtttacggt aacagcgac caaatcaac caacaagtc cggcaattc caaggcgga agacttaga gttgaagta cgagatggg aaagaatgg ttaccgagg agtactaac gctagtggc gtttgttct attcaaagc ccacaagaa gtttgctat ttagctggg gacgctcga gtcaaccaa aacccacaa ttgacaatc cttcaaatc attcttatg agagaacac aatcgaatt gctgacgct ttggccaaa ttgaatcca cattgggac gacgaaact attttccag gaagctaga agaattgca atcgccgaa caccaattt atttcatac tacgaatgg ttgccgatt ttcattgga attgaaaat tcgctcaag aacaaaatt atctatttg agcaaacat tttatcaac gattatcga caagaagtt gacccgacg gtgcttaac gaacacgca acggccgct ttcagatat ttccattcg ctaattgca gggcatttg gatttggta aacgagcac cgcagcagc tacggcaac ttgcgtctc agtgattgg tttaaccgt cctgctatt gtcgaacag ggcgacaat ttcgatgaa ttgactcga ggtttggcc actcagccg gaactcgct tctgatcct taccatgac agtgagata acacagttt ttgttccga gacggacaa caattcggt tcggatttg aaagccatt gatatccaa cgtaatcgc gaccatgga cttgcctcc tataacgaa tatcgaggt ttttgtgga ttgcccaga gcccacagt tttgaagat ttcctcgat gttatgaca caagacaat gtgaaaaaa ttggcgaca ctgtacgaa agtcccgac gatgttgat ttaacagtt ggtggttcg ttggaagcc catgttccg ggtacttta tcaggacca acattcttg tgtattttg actgaacag ttctataga actcgagtt ggggatagg ttttggttc gagaatcaa cacaacgga ttcacttta gcacaatta aacgcaatt cgtaaaacg agcatttcg agattatta tgcgacaat ggccatcac atccaagcc atgcaacca agaggcttt gaaagaata tctcacgat aatccgatt acaccgtgc gaaaatttg ccagctgtt gatctcact ttgtggaag gaagttccg tttggtgtc gaacgtccg tacgatttc gggtcggac ttttccgat gaattcttt tggaagaag aaataa
- >TC000175 RA
ttgtgtttg tggtgctcg acaaaacca ggattattt ttttgacat agtttgtag gaaattaag agtcaccat gtggtactt cctggccct tctccactt gaccttagc tgaacccaa aggctcggg ttatagcat tttcctcga acacacgcc aagttggga caggagacc ttacggtta cggacctgc ggcaccagt ggcctttga ccctttcaa caataacaa tttcttgcc acaaccaca accacaacc cccaacacc cgatccggc aaccttagg ctgtggtac cccaccagc agggtgccc caaatcgag gtaccgctc ctacgatgg ttcctgtaa taacttagg gaacccaat tttgggtac tccacagac acgatacgc gaggcttct accggcgaa ttacgccga tggggtgag cacccccac agcttcagt aacgggaaa accgctccc aggctctcg aatagtcag tcttttgat ttatcccga tgtacaaat tgaagatcc caaatttac aattaatgc catgcaata tgggcaaat aatcaccca tgatatgag catgattgc gggaagtac ccaagcaca gccccatca gaccaagtg ttgtacggc cgatggtca attgctcga gtttgccaa cataccgga acattgttt ccctatagt gttgcccaa cgatgaccc cgctcactc ccagacgaa tgcaaagtg catgaattt tgtaagaac gatcactga tcgtgatcg caactgtgt tggaggctc acaaccggc tgaacaatt aactgcagt taaccatta cttggactt atcgatagt ttacggtaa cagcgacca aatcaacca acaagtccg gcaattcca aggcggaag acttagagt tgaagtacg agatgggaa agaatggtt accgaggag tactaacgc tagtggcgt ttgttctat tcaaagccc acaagaagt ttgctattt agctgggga cgctcgagt caaccaaaa cccacaatt gacaatcct tcaaatcat tcttatgag agaacacaa tcgaattgc tgacgcttt ggccaaatt gaatccaca ttgggacga cgaaactat tttccagga agctagaag aattgcaat cgccgaaca ccaatttat ttcatacta cgaatggtt gccgatttt cattggaat tgaaaattc gctcaagaa caaaattat ctatttgag caaacattt tatcaacga ttatcgaca agaagttga cccgacggt gcttaacga acacgcaac ggccgcttt cagatattt ccattcgct aattgcagg gcatttgga tttggtaaa cgagcaccg cagcagcta cggcaactt gcgtctcag tgattggtt taaccgtcc tgctattgt cgaacaggg cgacaattt cgatgaatt gactcgagg tttggccac tcagccgga actcgcttc tgatcctta ccatgacag tgagataac acagttttt gttccgaga cggacaaca attcggttc ggatttgaa agccattga tatccaacg taatcgcga ccatggact tgcctccta taacgaata tcgaggttt ttgtggatt gcccagagc ccacagttt tgaagattt cctcgatgt tatgacaca agacaatgt gaaaaaatt ggcgacact gtacgaaag tcccgacga tgttgattt aacagttgg tggttcgtt ggaagccca tgttccggg tactttatc aggaccaac attcttgtg tattttgac tgaacagtt ctatagaac tcgagttgg ggataggtt ttggttcga gaatcaaca caacggatt cactttagc acaattaaa cgcaattcg taaaacgag catttcgag attattatg cgacaatgg ccatcacat ccaagccat gcaaccaag aggctttga aagaatatc tcacgataa tccgattac accgtgcga aaatttgcc agctgttga tctcacttt gtggaagga agttccgtt tggtgtcga acgtccgta cgatttcgg gtcggactt ttccgatga attcttttg gaagaagaa ataaattgt tgtgattgt ctcagacaa ttgttttgt gcctaaata tttttattt gtttttatt tgtacattt tctagttaa gttcatgtt aatactagg ctttatttg atagaactt cgaaagtcg aatattatt tgcacttta atttatgtt ttatctaat tttgtatat ttatgacag tattaataa aaaactcta ttttcg
