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Gene information
General information
TC000165 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid12: 136463..141273
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000165
    iBeetle screen iB_00031
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000165 RA
      MLDLFRASL LVILLVSVE ARTRRSIDL IAAHSLISD 
      TRLPREVTP NFYDLQLEP RPNEGTFEG RVRINVTWQ 
      EPTDKITLH AHEDLDIFK IIVTQFAPD DETEKASST 
      PLPPTEVRV MRIERVPKK PLYIIHLEK TANRGTQCI 
      VDLTFKGNI SNDTSEGIF RSSYVDPET KNRKWFVAT 
      HLRPNLARK VFPCFDEPA YKVPFVITV ARQKNMVAL 
      SNMPLKNSE QMKEKSDWV WDQFQQTPP ISTFSVGIV 
      VSEFSNTDY NTTDEDNNW NYEIRVWAR PDFITALAN 
      VTDKIRKSV KILEEFWGC PYPLPKLDV LALPNYQAT 
      RPADNWGLL LFKESELSG RGSWHISQE LTYQWLGSL 
      ATPFWWSDA HINNALVRY VTAYTTLKM ETNESFNNW 
      PMTMLYSIY YEFSKRYPH GKSTAIKQD STSAKTELV 
      FRMLNYTLG ERTFKHGLQ KFMADRQYK TFFGDDIWN 
      ALTEQAKFD NTLNESTVN AIAASWITK DRLPVVNVT 
      RNYKEKSAK ISQRVYLRE RPHDVPDKE KFVWWIPVI 
      LIRQDSLNF SNTKPIVWM KKEKEITLE NLPEANQFI 
      IVNPEEIGP FPVNYDVQN WNMLAQFLC SENRAKIPV 
      NTRAKLLHD AWNLAYAGD LSFATALNM TLFLKNERE 
      YLAWDPVFT LIDHIGRHI DSSAVHKKF QTYVRLLLT 
      PLYEELGEE PQEGESEGK AHLRSSSKV FLCQAGYKP 
      CIEEAQAAF RKWMESENP DEGNPVSNQ YICPVFKWG 
      TSEEWEFGL QRVIKFPAN RKQNERTYL LKTLAGCPN 
      EAEKIERLL NITILEQNG NFTDNDIYL IFSMLTGSA 
      NGYTTLFNF LKKNWDTIK ERFEGKLHL WNSLITSAT 
      TVFKTQEGL DMVSELYVE RQTEFGTAD FVIEKALKN 
      IKEETKWSN DNLPVIEKW LDEYLANNS K
    CDS sequence
    • >TC000165 RA
      atgttggat ctttttagg gcttccctc ttggtaata 
      ttgctcgta tcggtggaa gctcgaact cgccgtagc 
      atagattta atcgcagct cacagttta atctccgac 
      acacggctt cctcgtgaa gtcacccca aacttttac 
      gacttacaa ctcgaaccg cgaccaaat gaaggtact 
      tttgaaggc cgagttcga atcaatgtc acttggcaa 
      gaaccaact gacaaaatt acgctacat gcacacgaa 
      gatttggac atttttaaa atcatcgtc acacaattt 
      gcaccagat gatgaaacc gagaaagca tcatcgact 
      cctctacca ccaactgaa gtgagggta atgaggatc 
      gaaagagtg ccaaaaaaa ccgctctat atcatccat 
      ttagagaaa actgcaaat agagggacc cagtgcatc 
      gtggacctt actttcaag ggaaatatt agtaatgac 
      acaagtgag ggcattttc aggagtagt tatgtggat 
      ccagagacc aaaaaccga aagtggttt gttgcgaca 
      catttaaga ccaaatttg gcacgaaaa gtgtttcct 
      tgtttcgac gaacccgct tataaagtc ccttttgtg 
      attacagta gcaaggcaa aaaaatatg gtagctctc 
      tcgaacatg cctttgaaa aatagcgaa caaatgaaa 
      gagaaaagt gattgggtt tgggatcag ttccagcaa 
      acacctcca atatccaca ttttcagtt ggtattgtc 
      gtatccgaa ttcagcaac actgactat aacacaact 
      gacgaagac aacaattgg aactacgaa attcgggtc 
      tgggccagg cctgacttt atcacagct ttggccaat 
      gtcactgat aagattcga aagtcggtt aaaatccta 
      gaagagttc tgggggtgt ccttatccg ctcccaaaa 
      ctcgatgtt ttagccctt ccgaattat caagcgaca 
      aggcctgcc gataactgg ggtttactc cttttcaag 
      gaaagtgag ttaagtggt cgtggctct tggcacatt 
      tcgcaagaa ttgacctat cagtggtta ggctctctt 
      gcaacgcct ttttggtgg agtgatgct catattaat 
      aatgcgctg gttagatat gtgacagcc tatacaacc 
      ctcaaaatg gaaacgaac gaatcgttc aacaattgg 
      ccaatgaca atgttatac tcaatttat tacgagttc 
      agtaaacgt tacccacat ggcaaaagt actgcaatc 
      aaacaagac tcaacatcg gccaaaact gagctagtt 
      ttccgcatg ttaaactac acccttggc gaacgcaca 
      ttcaaacac ggattgcaa aaattcatg gctgatcga 
      cagtacaag acgttcttt ggtgatgat atttggaac 
      gctttgacc gaacaagca aaattcgac aatacttta 
      aacgaatca acagtcaac gcaattgca gcttcgtgg 
      atcacaaaa gaccgacta cctgttgta aacgttacg 
      cgaaactat aaagaaaaa tcggcgaaa atcagccaa 
      agggtttac ttgagagag cgtccacat gacgttccc 
      gataaggaa aaatttgtt tggtggatt cctgtgatt 
      ttaatccgt caagacagt ctcaatttt tccaacacg 
      aagccaatt gtttggatg aaaaaagag aaagaaatc 
      acccttgaa aatctacca gaggcaaac cagtttatt 
      atagttaat ccggaggaa atcggtccg tttccggtc 
      aattacgac gtgcaaaat tggaacatg ttggcacag 
      tttttgtgt tccgaaaac cgggcgaaa atcccggtg 
      aatacccgg gctaaactt ttacacgat gcttggaat 
      ttggcctat gctggagac ttgagcttt gctacagct 
      ttaaacatg acactgttt ctcaaaaat gaaagagaa 
      tatttggct tgggaccca gtttttact ctcattgat 
      cacattgga cgacatatc gacagttcc gcagttcat 
      aaaaaattc caaacttat gtgagattg ttgttaact 
      ccattgtac gaagagttg ggtgaggag cctcaagag 
      ggtgaaagt gaaggtaag gcacacttg aggagctcc 
      tcaaaagtg ttcttgtgc caagctggg tataaacca 
      tgcattgag gaagcacaa gctgccttc aggaagtgg 
      atggagagt gaaaaccca gatgaaggc aatcctgtt 
      tcgaatcag tacatttgt ccagtgttt aaatggggc 
      actagtgag gaatgggag tttggtttg caacgtgtt 
      attaaattc ccagccaac aggaagcaa aacgagcgc 
      acttatttg ctcaaaact ttagcagga tgtcctaat 
      gaagctgag aaaattgaa agattgctc aatattacg 
      attttggag caaaacgga aactttacg gacaacgat 
      atttatctc atttttagt atgttgaca ggaagtgca 
      aatggctat accacacta ttcaatttc ttgaagaag 
      aattgggat acgattaaa gagagattc gagggcaaa 
      cttcacttg tggaacagt ctgataaca tcagcaacg 
      acagttttc aaaacacag gaaggtctt gatatggtt 
      tcggaattg tacgttgaa cgacaaacc gaattcgga 
      acagcggac tttgtcatc gaaaaggcc ttaaagaac 
      atcaaagaa gagacgaaa tggagcaac gataacctc 
      cctgttatc gaaaaatgg ttagacgag tatttagcc 
      aacaactcg aaataa
    mRNA sequence
    • >TC000165 RA
      aaatcaatt cttaaaaat ggcactttt gggcaaatg 
      aattttctt tgcagactg aaaaatgtt ggatctttt 
      tagggcttc cctcttggt aatattgct cgtatcggt 
      ggaagctcg aactcgccg tagcataga tttaatcgc 
      agctcacag tttaatctc cgacacacg gcttcctcg 
      tgaagtcac cccaaactt ttacgactt acaactcga 
      accgcgacc aaatgaagg tacttttga aggccgagt 
      tcgaatcaa tgtcacttg gcaagaacc aactgacaa 
      aattacgct acatgcaca cgaagattt ggacatttt 
      taaaatcat cgtcacaca atttgcacc agatgatga 
      aaccgagaa agcatcatc gactcctct accaccaac 
      tgaagtgag ggtaatgag gatcgaaag agtgccaaa 
      aaaaccgct ctatatcat ccatttaga gaaaactgc 
      aaatagagg gacccagtg catcgtgga ccttacttt 
      caagggaaa tattagtaa tgacacaag tgagggcat 
      tttcaggag tagttatgt ggatccaga gaccaaaaa 
      ccgaaagtg gtttgttgc gacacattt aagaccaaa 
      tttggcacg aaaagtgtt tccttgttt cgacgaacc 
      cgcttataa agtcccttt tgtgattac agtagcaag 
      gcaaaaaaa tatggtagc tctctcgaa catgccttt 
      gaaaaatag cgaacaaat gaaagagaa aagtgattg 
      ggtttggga tcagttcca gcaaacacc tccaatatc 
      cacattttc agttggtat tgtcgtatc cgaattcag 
      caacactga ctataacac aactgacga agacaacaa 
      ttggaacta cgaaattcg ggtctgggc caggcctga 
      ctttatcac agctttggc caatgtcac tgataagat 
      tcgaaagtc ggttaaaat cctagaaga gttctgggg 
      gtgtcctta tccgctccc aaaactcga tgttttagc 
      ccttccgaa ttatcaagc gacaaggcc tgccgataa 
      ctggggttt actcctttt caaggaaag tgagttaag 
      tggtcgtgg ctcttggca catttcgca agaattgac 
      ctatcagtg gttaggctc tcttgcaac gcctttttg 
      gtggagtga tgctcatat taataatgc gctggttag 
      atatgtgac agcctatac aaccctcaa aatggaaac 
      gaacgaatc gttcaacaa ttggccaat gacaatgtt 
      atactcaat ttattacga gttcagtaa acgttaccc 
      acatggcaa aagtactgc aatcaaaca agactcaac 
      atcggccaa aactgagct agttttccg catgttaaa 
      ctacaccct tggcgaacg cacattcaa acacggatt 
      gcaaaaatt catggctga tcgacagta caagacgtt 
      ctttggtga tgatatttg gaacgcttt gaccgaaca 
      agcaaaatt cgacaatac tttaaacga atcaacagt 
      caacgcaat tgcagcttc gtggatcac aaaagaccg 
      actacctgt tgtaaacgt tacgcgaaa ctataaaga 
      aaaatcggc gaaaatcag ccaaagggt ttacttgag 
      agagcgtcc acatgacgt tcccgataa ggaaaaatt 
      tgtttggtg gattcctgt gattttaat ccgtcaaga 
      cagtctcaa tttttccaa cacgaagcc aattgtttg 
      gatgaaaaa agagaaaga aatcaccct tgaaaatct 
      accagaggc aaaccagtt tattatagt taatccgga 
      ggaaatcgg tccgtttcc ggtcaatta cgacgtgca 
      aaattggaa catgttggc acagttttt gtgttccga 
      aaaccgggc gaaaatccc ggtgaatac ccgggctaa 
      acttttaca cgatgcttg gaatttggc ctatgctgg 
      agacttgag ctttgctac agctttaaa catgacact 
      gtttctcaa aaatgaaag agaatattt ggcttggga 
      cccagtttt tactctcat tgatcacat tggacgaca 
      tatcgacag ttccgcagt tcataaaaa attccaaac 
      ttatgtgag attgttgtt aactccatt gtacgaaga 
      gttgggtga ggagcctca agagggtga aagtgaagg 
      taaggcaca cttgaggag ctcctcaaa agtgttctt 
      gtgccaagc tgggtataa accatgcat tgaggaagc 
      acaagctgc cttcaggaa gtggatgga gagtgaaaa 
      cccagatga aggcaatcc tgtttcgaa tcagtacat 
      ttgtccagt gtttaaatg gggcactag tgaggaatg 
      ggagtttgg tttgcaacg tgttattaa attcccagc 
      caacaggaa gcaaaacga gcgcactta tttgctcaa 
      aactttagc aggatgtcc taatgaagc tgagaaaat 
      tgaaagatt gctcaatat tacgatttt ggagcaaaa 
      cggaaactt tacggacaa cgatattta tctcatttt 
      tagtatgtt gacaggaag tgcaaatgg ctataccac 
      actattcaa tttcttgaa gaagaattg ggatacgat 
      taaagagag attcgaggg caaacttca cttgtggaa 
      cagtctgat aacatcagc aacgacagt tttcaaaac 
      acaggaagg tcttgatat ggtttcgga attgtacgt 
      tgaacgaca aaccgaatt cggaacagc ggactttgt 
      catcgaaaa ggccttaaa gaacatcaa agaagagac 
      gaaatggag caacgataa cctccctgt tatcgaaaa 
      atggttaga cgagtattt agccaacaa ctcgaaata 
      aacatttat tttatcagt aaaacatat ttattgtat 
      aaagttgta aaatacaat atttaacac aggacccag 
      ttttacttc accccaaag acaatccaa ttttttgct 
      taaaaaaat tctagctgc ttaattaaa aatcatttc 
      ttgcaacaa gctacagtt actttaatc caggaatat 
      ttcttagct tattctaga gtaattaaa tttcagaac 
      aaagatgta acaaaacgc aagttaatt tagagtatt 
      tggccattt ttcaatttt tttgacaaa atctgacaa 
      aatagcaaa gttttaggt tccaaaact gattaaaaa 
      attgactaa aatagacaa aagtaa