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Gene information
General information
TC000162 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15222580..15226009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000162
    iBeetle screen iB_00030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present # of remaining segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000162 RA
      MGYKAAVQI LKQAEDLKT KVLKKSDLL SDSQAWTDQ 
      QKLQNIYHQ ALVLDLEYA LDKKVEQDL WNIGFKNHI 
      SVLQELTRD KKNVRRSEC QALLTWCLE AASGFYLTL 
      LHELCNVYD IDLPFRRRG NVYGETNSN ELNLPQSSS 
      CVYICQYCL VHLGDIARY RNQRKQAEN FYKQAILVS 
      PTSGHPYNQ LALLEASQG DKLSTVFYY IRGIAVKNP 
      FPASTTNLL STLSSSLDK ESPLEKIHS KITVTEFIQ 
      IFLCTHALL HTATDLNQA EVGVKSLNT TMTALVATQ 
      SFTREKLIK ITAINLYAL KHVTSTSLT DELTEDEKI 
      VRQQILDLL AGSLSAFLI PVHTLKNDE SLLDYYALP 
      AVKLLTYWI MNESEILNE VVFTNRLQI WPSLCKLLN 
      NVQQYMKNF DYTKYANVP LFEDKLLQG FRPLIKNFK 
      ELDFKAGVE DEKTDNLIR MQRLIDFGL FISKLDVNH 
      TKLITTSTE SDLINFEPG CIQPDPTND LLEEMKSFH 
      VTDISTIPN KKGFEKRGG ILKPQGSLE KSREERELL 
      SSGEQSLMS DLSNSSASS SSSKKDISK NKRSKQNIA 
      LQSIFKKLE ENKQVKFKV EEKEKLVTF DTKPSPFNP 
      PLRTQSFNQ NHPAFPIPP PPPPSQPDY LNTLRNVTK 
      PPQPNSFVF PPKTENPSM VPFQNNFNQ RVYQFPGGN 
      PYKMEETNS PWWPNTSQN YQQRPDSFV NYPFSNNMP 
      YVPDYNKTQ NNQAAGDMF NSPWNTFGR TNLLESNMN 
      YENLSMRQT MLKEATPPS RPSHDNFMQ NPTTSPGYS 
      LFNTNTWTP NLVGQLRNN KAQDNQVSH LPQHSLFTG 
      QGPQSLAQL LEQQQSQKN DV
    CDS sequence
    • >TC000162 RA
      atgggttat aaagcagct gttcaaatt ctaaaacaa 
      gccgaagac ttaaaaacc aaggtatta aaaaaaagc 
      gatttatta tcggattcg caagcatgg acggaccaa 
      cagaaattg caaaatatt taccatcaa gcattggta 
      cttgatttg gaatacgct ctcgacaag aaagtggag 
      caagacttg tggaacatt ggttttaag aatcacatc 
      tcggtgtta caagagttg actcgcgat aaaaaaaac 
      gtcagaaga tctgaatgt caggcactt ttaacttgg 
      tgtttagaa gcggcttct gggttttat ttgacactt 
      ttgcacgaa ttgtgcaac gtatatgac attgattta 
      ccgttcaga agacgcgga aatgtttat ggggaaaca 
      aattcaaat gaactcaat ttgcctcaa tcgtcctcc 
      tgtgtgtac atttgccaa tactgcttg gtacatttg 
      ggagatatt gcacgttat agaaaccaa agaaagcaa 
      gctgaaaat ttttacaag caagcaatt ttagtttct 
      ccaactagt ggtcatcct tacaatcag ttggctctt 
      ttggaggca agtcaaggt gataaatta tcaactgtc 
      ttttattac attaggggc attgctgtg aaaaatccc 
      ttcccagca tccaccact aatctatta agcacttta 
      agttcgtcg cttgataaa gaaagtcca ttagaaaaa 
      attcacagt aaaataacg gtaaccgag tttattcaa 
      atttttttg tgcactcac gccctcttg cacactgcc 
      accgattta aaccaagct gaagtcggg gttaaaagt 
      ttaaataca acaatgaca gctttagta gcgactcaa 
      agttttaca agagagaaa ttaataaaa attactgcg 
      attaatttg tacgcttta aaacatgta acttcaaca 
      tcactcact gatgaatta accgaagat gaaaaaatt 
      gtcagacaa caaattttg gatttgttg gctgggtct 
      ttgagcgcg tttcttatt ccagttcat acgctaaaa 
      aatgacgaa agcttgttg gattattac gcacttcct 
      gcagtgaaa cttttaaca tattggatt atgaacgaa 
      tcggaaatt cttaacgaa gttgttttt actaatcgg 
      ttgcaaatt tggcccagt ttgtgcaaa cttttgaat 
      aacgtacag caatacatg aaaaacttt gactacacc 
      aaatatgcc aatgtgcct ctgtttgaa gataaattg 
      ttgcaaggc tttcggcct ttgataaaa aatttcaaa 
      gagttggat tttaaggcg ggtgtcgag gatgaaaaa 
      actgataat ttaatccga atgcagcgt ttgattgat 
      tttggttta tttatcagc aagttggat gtgaatcat 
      acgaaatta attacgaca agtaccgaa agtgattta 
      attaatttt gaacctgga tgcattcag ccagaccca 
      actaatgac ttattggag gaaatgaaa tcatttcat 
      gtcactgat atttcaaca attcctaat aaaaaaggt 
      tttgaaaaa cggggcggt attttaaaa ccacaaggt 
      tccttggaa aaatcgcgc gaagaacgc gaattatta 
      tcatcggga gaacaaagt ctcatgtcc gatttaagt 
      aattcaagt gcgtcctca agtagtagc aaaaaagac 
      atttcaaaa aataaacga tccaagcaa aacattgct 
      ctgcagtcg atttttaaa aaactggaa gagaataaa 
      caggtgaaa tttaaagtt gaagaaaaa gaaaaattg 
      gtgactttt gacacaaag ccttctccg tttaatccc 
      cctttgcga acccaatcc ttcaaccag aaccatcca 
      gctttccct attcctcct cctcctcct cccagccaa 
      cctgattac ctcaatact ctccggaac gtaacgaaa 
      cctccacag ccaaattca tttgttttc cctcccaaa 
      actgagaat ccatcaatg gttccattt caaaataat 
      ttcaatcag cgtgtctat caatttccc gggggaaac 
      ccctacaaa atggaggaa acgaattcg ccttggtgg 
      cccaacacg tcacagaat taccaacaa cgacctgat 
      agtttcgta aattatccc tttagcaat aatatgccc 
      tatgtgccc gattataat aagacgcaa aataatcaa 
      gcagccggc gatatgttt aattcacca tggaatact 
      tttggaagg actaactta cttgaaagc aacatgaat 
      tatgaaaat ttgtcgatg agacagacg atgcttaaa 
      gaggcgaca ccaccttca agacctagt catgataat 
      ttcatgcaa aatcccaca acatctcct ggttattca 
      ttgtttaat accaacact tggacgccg aatttagtt 
      ggacagttg aggaataac aaggcacag gataatcaa 
      gtgagtcat ttgcctcaa cattccttg tttactgga 
      caaggaccc caatcgtta gcccaatta ttggaacag 
      caacagtcg caaaaaaat gatgtttag
    mRNA sequence
    • >TC000162 RA
      attcaaact tcatcttaa cctcaaact cgcaaaatt 
      gttgatttc aggtacaca ttttcgctt ttaatttat 
      tccagtgat ggatattca tgatatcag gtcataccc 
      aataatttc tacaccttg atacattta cccaaacta 
      tcaaattga gaatgggtt ataaagcag ctgttcaaa 
      ttctaaaac aagccgaag acttaaaaa ccaaggtat 
      taaaaaaaa gcgatttat tatcggatt cgcaagcat 
      ggacggacc aacagaaat tgcaaaata tttaccatc 
      aagcattgg tacttgatt tggaatacg ctctcgaca 
      agaaagtgg agcaagact tgtggaaca ttggtttta 
      agaatcaca tctcggtgt tacaagagt tgactcgcg 
      ataaaaaaa acgtcagaa gatctgaat gtcaggcac 
      ttttaactt ggtgtttag aagcggctt ctgggtttt 
      atttgacac ttttgcacg aattgtgca acgtatatg 
      acattgatt taccgttca gaagacgcg gaaatgttt 
      atggggaaa caaattcaa atgaactca atttgcctc 
      aatcgtcct cctgtgtgt acatttgcc aatactgct 
      tggtacatt tgggagata ttgcacgtt atagaaacc 
      aaagaaagc aagctgaaa atttttaca agcaagcaa 
      ttttagttt ctccaacta gtggtcatc cttacaatc 
      agttggctc ttttggagg caagtcaag gtgataaat 
      tatcaactg tcttttatt acattaggg gcattgctg 
      tgaaaaatc ccttcccag catccacca ctaatctat 
      taagcactt taagttcgt cgcttgata aagaaagtc 
      cattagaaa aaattcaca gtaaaataa cggtaaccg 
      agtttattc aaatttttt tgtgcactc acgccctct 
      tgcacactg ccaccgatt taaaccaag ctgaagtcg 
      gggttaaaa gtttaaata caacaatga cagctttag 
      tagcgactc aaagtttta caagagaga aattaataa 
      aaattactg cgattaatt tgtacgctt taaaacatg 
      taacttcaa catcactca ctgatgaat taaccgaag 
      atgaaaaaa ttgtcagac aacaaattt tggatttgt 
      tggctgggt ctttgagcg cgtttctta ttccagttc 
      atacgctaa aaaatgacg aaagcttgt tggattatt 
      acgcacttc ctgcagtga aacttttaa catattgga 
      ttatgaacg aatcggaaa ttcttaacg aagttgttt 
      ttactaatc ggttgcaaa tttggccca gtttgtgca 
      aacttttga ataacgtac agcaataca tgaaaaact 
      ttgactaca ccaaatatg ccaatgtgc ctctgtttg 
      aagataaat tgttgcaag gctttcggc ctttgataa 
      aaaatttca aagagttgg attttaagg cgggtgtcg 
      aggatgaaa aaactgata atttaatcc gaatgcagc 
      gtttgattg attttggtt tatttatca gcaagttgg 
      atgtgaatc atacgaaat taattacga caagtaccg 
      aaagtgatt taattaatt ttgaacctg gatgcattc 
      agccagacc caactaatg acttattgg aggaaatga 
      aatcatttc atgtcactg atatttcaa caattccta 
      ataaaaaag gttttgaaa aacggggcg gtattttaa 
      aaccacaag gttccttgg aaaaatcgc gcgaagaac 
      gcgaattat tatcatcgg gagaacaaa gtctcatgt 
      ccgatttaa gtaattcaa gtgcgtcct caagtagta 
      gcaaaaaag acatttcaa aaaataaac gatccaagc 
      aaaacattg ctctgcagt cgattttta aaaaactgg 
      aagagaata aacaggtga aatttaaag ttgaagaaa 
      aagaaaaat tggtgactt ttgacacaa agccttctc 
      cgtttaatc cccctttgc gaacccaat ccttcaacc 
      agaaccatc cagctttcc ctattcctc ctcctcctc 
      ctcccagcc aacctgatt acctcaata ctctccgga 
      acgtaacga aacctccac agccaaatt catttgttt 
      tccctccca aaactgaga atccatcaa tggttccat 
      ttcaaaata atttcaatc agcgtgtct atcaatttc 
      ccgggggaa acccctaca aaatggagg aaacgaatt 
      cgccttggt ggcccaaca cgtcacaga attaccaac 
      aacgacctg atagtttcg taaattatc cctttagca 
      ataatatgc cctatgtgc ccgattata ataagacgc 
      aaaataatc aagcagccg gcgatatgt ttaattcac 
      catggaata cttttggaa ggactaact tacttgaaa 
      gcaacatga attatgaaa atttgtcga tgagacaga 
      cgatgctta aagaggcga caccacctt caagaccta 
      gtcatgata atttcatgc aaaatccca caacatctc 
      ctggttatt cattgttta ataccaaca cttggacgc 
      cgaatttag ttggacagt tgaggaata acaaggcac 
      aggataatc aagtgagtc atttgcctc aacattcct 
      tgtttactg gacaaggac cccaatcgt tagcccaat 
      tattggaac agcaacagt cgcaaaaaa atgatgttt 
      agttttata gataggtgt attatagta gttacttgg 
      tcctagtga aataatgga ttcgtaaca gttaataaa 
      tcagttttg ttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY