Status
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Gene information
General information
TC000158 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15259958..15262459
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000158
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000158 RA
      MDKRQSNNQ VGRKRKSKS CRKEKKWKC SNATKSHLF 
      KEATFDSSS DENNVSCVK LSDSFKFTM SLDLKPSKS 
      EMDEAIENE LFNAEAPTL SEYSFKTVS DEEEDELHN 
      SVVDEFKDE SVLDESSVI VSVDSNRVE KNEKKHKVS 
      QPRLENEFG KNVQYCYTS DGIVVVLDK NEIVYIHGL 
      CLLSVLHGQ VEILGHIMD KNSGEAKIY SPRGSALLF 
      LKNVTESDV PAPNYLLNL KISTIKLTQ HCAVVLCKQ 
      LNDSNISFI EKHISQQIL QRCDENDHL PRITFHPQE 
      GSWNLIKTD KSWEKIVDT ITPHTKMMI CGGKGVGKT 
      TFLRYALNR LLMKFKKIR VIDLDPGQA EFTVPGCVS 
      VIEITEPVF GVNYTHLQK AQRSILSNI NIAYEPDKY 
      IASIKELLL EKHFEEIPT LVNYMGYTH GMGLNILSA 
      VIIHVQPTD ILQISSRDS KKNYKSVLE TDIVAEKAQ 
      LFVSNLKNL HYNLHQIES KCDENDGWT AEPRQLREM 
      TILAYIREM INSDSVFKT NAPLCRISL NDVSITDLN 
      GNEIPPAAV NACFVALCK QVAEMPHF
    CDS sequence
    • >TC000158 RA
      atggacaaa aggcagtca aataatcaa gttgggcgt 
      aaaagaaag tcaaaaagt tgtcgaaaa gagaagaaa 
      tggaaatgt tcaaatgcc acgaaaagt catttgttc 
      aaagaagct acttttgat tcatcgagt gatgaaaac 
      aacgtatct tgtgttaaa ttatctgat tcgtttaaa 
      tttactatg agtttggat ctaaaacca tcaaaatct 
      gaaatggac gaagctatt gaaaatgaa ttatttaat 
      gctgaagct ccgacttta agtgaatat agttttaag 
      actgtatca gatgaagag gaagacgaa ttgcataat 
      tcggttgtt gacgaattt aaagatgaa agtgtatta 
      gatgaaagt tcagtaatt gtgtcggtg gattcaaac 
      cgtgtcgaa aaaaacgaa aaaaaacac aaagtttcg 
      cagccaagg cttgaaaat gagttcggt aaaaacgtt 
      caatattgt tacacaagt gatggaatt gttgttgta 
      ttggacaaa aacgaaatt gtgtacatc catggcctt 
      tgtttactt tccgtttta catggccaa gttgaaatt 
      ttagggcac ataatggat aaaaacagt ggagaagcg 
      aaaatctat tcacctcga ggcagtgca cttctcttt 
      ttgaaaaat gtaaccgaa tcagatgtg cctgcacct 
      aattattta ctcaatttg aaaatttca acgattaaa 
      ttaacacaa cattgtgcg gtagtcctt tgcaaacag 
      ctcaatgat tcaaatatt tcatttatc gagaaacac 
      atttctcaa caaattctt caaaggtgt gacgaaaac 
      gaccattta ccacgaatt acgtttcat ccacaagaa 
      ggcagttgg aatttaatt aaaaccgat aagagctgg 
      gagaaaatt gttgataca ataacgccg catacgaaa 
      atgatgatt tgtggtgga aaaggagtg ggaaaaaca 
      acttttttg cgttatgca ttaaacagg cttttaatg 
      aaatttaaa aaaatcaga gtgattgat ttagatcca 
      ggccaggcc gagttcact gttccaggt tgtgtttca 
      gttattgaa attacggaa cctgttttc ggagtcaat 
      tacacgcat ttgcaaaaa gcacaaagg tcgattctt 
      tcaaatata aatattgcc tatgagcct gacaaatac 
      atcgctagt attaaggag ttgttgtta gaaaaacat 
      ttcgaggaa attccgact ctagtaaat tacatgggc 
      tatacacat ggcatggga cttaatata ctttcagcc 
      gtaatcatt catgttcaa ccaacagat attttacaa 
      atctctagt cgcgattca aagaaaaat tataagagt 
      gttttagaa acggatatt gtagcggaa aaagcacaa 
      ttgtttgtt tcaaatttg aaaaatctt cattataat 
      ttacatcaa attgaatca aagtgtgat gaaaatgat 
      ggttggact gctgaacca agacaatta agagaaatg 
      actatcttg gcttatata cgcgagatg attaactct 
      gattcagtt tttaaaaca aacgctcca ctatgcaga 
      ataagttta aatgatgta agcattact gatttgaac 
      ggaaatgaa atacctcca gcggctgta aatgcatgt 
      tttgttgcg ttatgtaaa caagtcgct gaaatgccc 
      catttttga
    mRNA sequence
    • >TC000158 RA
      aatgatccc ccaaatgca gaaacaact gttggctgc 
      cctgttgtt accatctgt caaattgat acacgtttt 
      tacaggtta aaaaaatac tggttgtta tattaaggg 
      aatgaaata caacttgat ttcttaagc attaactaa 
      aaatggaca aaaggcagt caaataatc aagttgggc 
      gtaaaagaa agtcaaaaa gttgtcgaa aagagaaga 
      aatggaaat gttcaaatg ccacgaaaa gtcatttgt 
      tcaaagaag ctacttttg attcatcga gtgatgaaa 
      acaacgtat cttgtgtta aattatctg attcgttta 
      aatttacta tgagtttgg atctaaaac catcaaaat 
      ctgaaatgg acgaagcta ttgaaaatg aattattta 
      atgctgaag ctccgactt taagtgaat atagtttta 
      agactgtat cagatgaag aggaagacg aattgcata 
      attcggttg ttgacgaat ttaaagatg aaagtgtat 
      tagatgaaa gttcagtaa ttgtgtcgg tggattcaa 
      accgtgtcg aaaaaaacg aaaaaaaac acaaagttt 
      cgcagccaa ggcttgaaa atgagttcg gtaaaaacg 
      ttcaatatt gttacacaa gtgatggaa ttgttgttg 
      tattggaca aaaacgaaa ttgtgtaca tccatggcc 
      tttgtttac tttccgttt tacatggcc aagttgaaa 
      ttttagggc acataatgg ataaaaaca gtggagaag 
      cgaaaatct attcacctc gaggcagtg cacttctct 
      ttttgaaaa atgtaaccg aatcagatg tgcctgcac 
      ctaattatt tactcaatt tgaaaattt caacgatta 
      aattaacac aacattgtg cggtagtcc tttgcaaac 
      agctcaatg attcaaata tttcattta tcgagaaac 
      acatttctc aacaaattc ttcaaaggt gtgacgaaa 
      acgaccatt taccacgaa ttacgtttc atccacaag 
      aaggcagtt ggaatttaa ttaaaaccg ataagagct 
      gggagaaaa ttgttgata caataacgc cgcatacga 
      aaatgatga tttgtggtg gaaaaggag tgggaaaaa 
      caacttttt tgcgttatg cattaaaca ggcttttaa 
      tgaaattta aaaaaatca gagtgattg atttagatc 
      caggccagg ccgagttca ctgttccag gttgtgttt 
      cagttattg aaattacgg aacctgttt tcggagtca 
      attacacgc atttgcaaa aagcacaaa ggtcgattc 
      tttcaaata taaatattg cctatgagc ctgacaaat 
      acatcgcta gtattaagg agttgttgt tagaaaaac 
      atttcgagg aaattccga ctctagtaa attacatgg 
      gctatacac atggcatgg gacttaata tactttcag 
      ccgtaatca ttcatgttc aaccaacag atattttac 
      aaatctcta gtcgcgatt caaagaaaa attataaga 
      gtgttttag aaacggata ttgtagcgg aaaaagcac 
      aattgtttg tttcaaatt tgaaaaatc ttcattata 
      atttacatc aaattgaat caaagtgtg atgaaaatg 
      atggttgga ctgctgaac caagacaat taagagaaa 
      tgactatct tggcttata tacgcgaga tgattaact 
      ctgattcag tttttaaaa caaacgctc cactatgca 
      gaataagtt taaatgatg taagcatta ctgatttga 
      acggaaatg aaatacctc cagcggctg taaatgcat 
      gttttgttg cgttatgta aacaagtcg ctgaaatgc 
      cccattttt gaatgcctg ggatggggt atgtagtaa 
      ttatctttg catacttat ctcagtgtc attgttttg 
      tttttcgca ggtattatt cgaggagtg gacgtaatt 
      aacaatttt ttggtgtta ctaacatca gaaaaattg 
      caaattttg gataaagtg agccattta gtaatgggt 
      ggaggagct gctttacct ccttcaata cacatgaca 
      ccggatgaa gtgactgga cctatacct tatgtaatc 
      gaaggcgtt ttaatgtct ttcggacaa ataccaaaa 
      cgctcccga atttctgct aaaaaataa aaatcaaat 
      aaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY