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Gene information
General information
TC000151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 26836..31983
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000151
    iBeetle screen iB_00027
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000151 RA
      MGLELQFSI INLLILLIT FWTTLYLYF TRKFNHWKS 
      KNVPQVAPI PFFGNAFEV FTWRKNIGE FARQIYNST 
      TKPFIGFFI CDEPYLLIR DPELVKSIL VKDFAVFSN 
      RSISENKEN DPMGSHLLF LLKTPDWRD MRRKITPVF 
      TSGKMKNMY SLISEAGND MIQHMRKEV SKSDQLEMR 
      EVAARYTTD AITSTSFGI NANCFKNEK AEFREVSRR 
      VFNWAIWER SISTTCYFI APNLVKLFK LKFIDSASA 
      TFLREAFWR TMTDREEKK FVRNDLLDI LIDIKKQED 
      INDPYKLDG DKLVAQATQ FFVAGFETT SSTICFTLY 
      ELAINKDLQ NKLKSEIRD VVRKHGEIS YNSLKDMEY 
      LDMCIKETL RKYPVLPFL DRKCDTDYR IPGSDVVLE 
      KGSPVFISV SGLHYDPQY FPDPDKYDP LRFTEENIK 
      SRPQFTYLP FGEGPRNCI GARFGSVSS KSGVAKIIS 
      EFEVDLCEK TQHPIQIDP KGFLMAPVS DLVLKFKRL 
      DD
    CDS sequence
    • >TC000151 RA
      atgggcctt gagttacaa ttctcgatt atcaactta 
      cttatctta cttatcaca ttttggaca actctctac 
      ttgtacttc actcgcaaa ttcaaccac tggaagagc 
      aaaaatgtg ccccaagtg gccccaatt ccatttttc 
      ggaaacgct ttcgaggtg ttcacttgg cgaaaaaac 
      atcggggaa ttcgcccgc caaatctac aacagtaca 
      accaaacca ttcatcggt ttcttcatc tgtgacgaa 
      ccctacttg ttaatccgc gacccggaa ctggtcaaa 
      tcaatcctc gtcaaagat ttcgcagtt ttcagcaac 
      cggagcatt tccgaaaac aaggaaaat gacccgatg 
      gggtcccac ttgcttttc cttttgaaa actccggac 
      tggagagac atgcgccgg aaaataact ccggttttc 
      acttccggg aaaatgaaa aatatgtat agtctgatt 
      agcgaagct gggaacgac atgatccag cacatgagg 
      aaagaagtg agtaaaagt gatcaactg gagatgaga 
      gaagtggcc gcgcgatat acaacagat gcgataaca 
      tcgacttcg tttggaatc aacgcgaat tgcttcaaa 
      aatgaaaaa gccgagttt agagaggtg tccaggcga 
      gtgtttaat tgggcaatt tgggagcgt tctatttcc 
      accacttgc tattttatc gcgcctaat ttagtaaaa 
      ttgtttaaa ttgaaattt attgattcg gcttcggcg 
      acttttctg cgagaggcc ttctggaga actatgacc 
      gatcgagaa gagaaaaag tttgtgcga aacgattta 
      ttggatatt ttgatcgat attaagaag caggaggat 
      attaatgac ccctacaag ttggatggt gataaattg 
      gtggctcaa gcaactcag ttctttgtg gctggtttt 
      gaaaccacg agttccaca atttgcttc actttgtac 
      gaattggcg attaataaa gatttgcaa aataaattg 
      aaaagtgaa attagagac gttgtgcgt aaacatgga 
      gaaatctcg tacaattcc ttgaaggat atggagtat 
      ttagacatg tgtattaaa gaaaccctg cgaaaatat 
      cccgtgttg cccttccta gatcgaaaa tgtgacaca 
      gattatcgc atcccagga tcagacgta gtcctggaa 
      aaaggatca cctgtgttt atatcagtc tctggactg 
      cactacgat ccacagtat tttccagat cctgataaa 
      tacgatcct ttgagattt actgaggaa aacattaaa 
      tcaagacca cagtttacg tatttgcct tttggtgaa 
      ggacctcgc aattgcatt ggtgcacgg ttcggtagc 
      gtgagttcc aaatccgga gttgctaaa ataatatca 
      gagtttgaa gtggatttg tgtgagaaa acgcaacac 
      ccgattcaa atcgatccc aaagggttt ttgatggca 
      ccagtttct gatcttgta cttaaattt aagagattg 
      gatgactaa
    mRNA sequence
    • >TC000151 RA
      acacataaa aagctatac atacgtgaa gtgcacttg 
      acgaggtta ctgaataat gggccttga gttacaatt 
      ctcgattat caacttact tatcttact tatcacatt 
      ttggacaac tctctactt gtacttcac tcgcaaatt 
      caaccactg gaagagcaa aaatgtgcc ccaagtggc 
      cccaattcc atttttcgg aaacgcttt cgaggtgtt 
      cacttggcg aaaaaacat cggggaatt cgcccgcca 
      aatctacaa cagtacaac caaaccatt catcggttt 
      cttcatctg tgacgaacc ctacttgtt aatccgcga 
      cccggaact ggtcaaatc aatcctcgt caaagattt 
      cgcagtttt cagcaaccg gagcatttc cgaaaacaa 
      ggaaaatga cccgatggg gtcccactt gcttttcct 
      tttgaaaac tccggactg gagagacat gcgccggaa 
      aataactcc ggttttcac ttccgggaa aatgaaaaa 
      tatgtatag tctgattag cgaagctgg gaacgacat 
      gatccagca catgaggaa agaagtgag taaaagtga 
      tcaactgga gatgagaga agtggccgc gcgatatac 
      aacagatgc gataacatc gacttcgtt tggaatcaa 
      cgcgaattg cttcaaaaa tgaaaaagc cgagtttag 
      agaggtgtc caggcgagt gtttaattg ggcaatttg 
      ggagcgttc tatttccac cacttgcta ttttatcgc 
      gcctaattt agtaaaatt gtttaaatt gaaatttat 
      tgattcggc ttcggcgac ttttctgcg agaggcctt 
      ctggagaac tatgaccga tcgagaaga gaaaaagtt 
      tgtgcgaaa cgatttatt ggatatttt gatcgatat 
      taagaagca ggaggatat taatgaccc ctacaagtt 
      ggatggtga taaattggt ggctcaagc aactcagtt 
      ctttgtggc tggttttga aaccacgag ttccacaat 
      ttgcttcac tttgtacga attggcgat taataaaga 
      tttgcaaaa taaattgaa aagtgaaat tagagacgt 
      tgtgcgtaa acatggaga aatctcgta caattcctt 
      gaaggatat ggagtattt agacatgtg tattaaaga 
      aaccctgcg aaaatatcc cgtgttgcc cttcctaga 
      tcgaaaatg tgacacaga ttatcgcat cccaggatc 
      agacgtagt cctggaaaa aggatcacc tgtgtttat 
      atcagtctc tggactgca ctacgatcc acagtattt 
      tccagatcc tgataaata cgatccttt gagatttac 
      tgaggaaaa cattaaatc aagaccaca gtttacgta 
      tttgccttt tggtgaagg acctcgcaa ttgcattgg 
      tgcacggtt cggtagcgt gagttccaa atccggagt 
      tgctaaaat aatatcaga gtttgaagt ggatttgtg 
      tgagaaaac gcaacaccc gattcaaat cgatcccaa 
      agggttttt gatggcacc agtttctga tcttgtact 
      taaatttaa gagattgga tgactaaat aaataagtg 
      tgtaacaat tcatttgag ttatttttt tgtctaaaa 
      aaataattg aaataaccc aggtgttaa caacaaagc 
      aacgttttt tcagatatt tcatgttgg actctggcg 
      agttcaata cacaagaaa attaagt