Status
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Gene information
General information
TC000144 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10595021..10599661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000144
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00026

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000144 RA
      MDSQGKKVI VCDNGTGFV KCGYAGSNF PAHIFPSMV 
      GRPIIRAVN KIGDIEVKD LMVGDEASA LRSLLEVNY 
      PMENGVVRN WEDMCHVWD YTFGPRKMN LDPTDAKIL 
      LTEPPMNPT KNREKMIEV MFEKYGFAG AYIAVQAVL 
      TLYAQGLLS GVVVDSGDG VTHICPVYE EFALPHLTR 
      RLDIAGRDI TRYLIKLLL LRGYAFNHS DDFETVRIM 
      KEKLCYIGY DIETEQRLA LETTVLVEP YILPDGRVI 
      KVGGERFEA PEALFQPHL INVEGQGIA ELVFNTIQA 
      ADIDMRPEL YKHIVLSGG STMYPGLPS RLEREIKQL 
      YLERVLKND IEKLNKFKI RIEDPPRRK DMVFIGGAV 
      LAEVMKDRD AFWMSKQEY EEQGLKVLK KLGPRSA
    CDS sequence
    • >TC000144 RA
      atggatagt caaggcaaa aaagtcatt gtttgtgac 
      aatggaact gggtttgtc aagtgtgga tacgcaggg 
      agcaatttc ccggcccat attttccct tcaatggtc 
      ggccgaccc atcattcgg gctgtaaat aaaatcgga 
      gatattgag gttaaggac ttgatggtt ggagatgaa 
      gcttccgct ttgcgatcg cttctcgag gtgaattac 
      ccgatggaa aatggggtt gtgcgcaac tgggaagat 
      atgtgccac gtgtgggac tacactttt ggcccccga 
      aaaatgaat ttggaccca actgatgct aaaattctc 
      ctgactgaa cccccaatg aaccccacg aaaaatcgg 
      gaaaaaatg atcgaagtc atgttcgaa aagtacggt 
      tttgcgggg gcttatatt gctgtccaa gccgtactc 
      actttatac gctcaggga cttctgtcg ggtgttgtt 
      gtcgattct ggtgacgga gtgacacac atttgcccc 
      gtttatgaa gagtttgct ttgccgcat ttaacccga 
      cgtctggat atagcaggc cgtgatatc acccgctat 
      cttattaaa ttattacta ctgagaggt tacgcattt 
      aatcattcg gatgatttt gaaaccgtg cgaattatg 
      aaggaaaaa ttatgttac attggttac gatatagaa 
      acagaacag cggttggct ttggaaact actgttttg 
      gttgagcct tatattttg ccagatggc cgtgtgatc 
      aaagttggc ggggaacgg tttgaggcc cctgaagca 
      ttatttcaa ccgcattta atcaacgtt gagggacaa 
      ggcatcgct gagcttgtt tttaataca atacaggcg 
      gctgatatt gacatgagg cccgaattg tacaaacat 
      atagtactc tcaggtggt tccactatg tatccaggg 
      ctcccttca cgacttgaa cgtgaaata aagcaattg 
      tatctagaa cgcgtactt aaaaatgac atagagaaa 
      ttaaataag tttaaaata cgaattgag gatccgccg 
      agacgtaaa gacatggtt tttatcggt ggagcagtt 
      ttagccgaa gttatgaag gatcgggat gcgttttgg 
      atgtcgaag caagagtat gaggagcaa ggcttgaag 
      gttttgaaa aaactgggg ccgaggtcg gcgtaa
    mRNA sequence
    • >TC000144 RA
      tcacaagca ccggtacaa aacgggtgg tttgtcaaa 
      tttttaatt gcattaatc aaaattaat aaacagaca 
      caattaaac aaacagcaa atcattcat cttttttca 
      cttgtgaca atttgtgat atgtgttcg atttatatc 
      tatgggcgt ggacagatt tcctggagt gactgttct 
      agtgtaatt atcccaaaa tagggctta aagactcct 
      cttccccta ccaattaac aattaagtt catttaatc 
      gcatcacca tggatagtc aaggcaaaa aagtcattg 
      tttgtgaca atggaactg ggtttgtca agtgtggat 
      acgcaggga gcaatttcc cggcccata ttttccctt 
      caatggtcg gccgaccca tcattcggg ctgtaaata 
      aaatcggag atattgagg ttaaggact tgatggttg 
      gagatgaag cttccgctt tgcgatcgc ttctcgagg 
      tgaattacc cgatggaaa atggggttg tgcgcaact 
      gggaagata tgtgccacg tgtgggact acacttttg 
      gcccccgaa aaatgaatt tggacccaa ctgatgcta 
      aaattctcc tgactgaac ccccaatga accccacga 
      aaaatcggg aaaaaatga tcgaagtca tgttcgaaa 
      agtacggtt ttgcggggg cttatattg ctgtccaag 
      ccgtactca ctttatacg ctcagggac ttctgtcgg 
      gtgttgttg tcgattctg gtgacggag tgacacaca 
      tttgccccg tttatgaag agtttgctt tgccgcatt 
      taacccgac gtctggata tagcaggcc gtgatatca 
      cccgctatc ttattaaat tattactac tgagaggtt 
      acgcattta atcattcgg atgattttg aaaccgtgc 
      gaattatga aggaaaaat tatgttaca ttggttacg 
      atatagaaa cagaacagc ggttggctt tggaaacta 
      ctgttttgg ttgagcctt atattttgc cagatggcc 
      gtgtgatca aagttggcg gggaacggt ttgaggccc 
      ctgaagcat tatttcaac cgcatttaa tcaacgttg 
      agggacaag gcatcgctg agcttgttt ttaatacaa 
      tacaggcgg ctgatattg acatgaggc ccgaattgt 
      acaaacata tagtactct caggtggtt ccactatgt 
      atccagggc tcccttcac gacttgaac gtgaaataa 
      agcaattgt atctagaac gcgtactta aaaatgaca 
      tagagaaat taaataagt ttaaaatac gaattgagg 
      atccgccga gacgtaaag acatggttt ttatcggtg 
      gagcagttt tagccgaag ttatgaagg atcgggatg 
      cgttttgga tgtcgaagc aagagtatg aggagcaag 
      gcttgaagg ttttgaaaa aactggggc cgaggtcgg 
      cgtaagcaa ttttaaata atatacgtg tttttattt 
      tcatattta attcattca gtgtatatt taaaggcaa 
      gttgaataa atcattttt aaacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY