Status
Please wait ...
Gene information
General information
TC032473
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000122,TC000123
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10856669..10880153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032473
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00025

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    vitellarium size decreased - Number of animals showing the phenotype: 3

    egg chamber number decreased - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupa lethality as pupa - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    remarks: animals lost, eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 6

    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032473 RA
      MDEFKIEFD ATLTDDYLL SDDIWKQFQ IEELQPFSS 
      SFDCSTTCS FLCDEKNCQ VKYDCMWNG HCKGAHGNR 
      SCNSKTTPG DVKPPVVQV PQQSLLKPD LKISVDPNT 
      VVPMMPSLH YSIQTPPIS DDEDTKPTQ ILQSLQSAL 
      SENELDDAD LSDYFKDDD AWGFFDDGL DEEEEEEEE 
      EEDEIDEER RKHENDMRA FYAATDHSY HKGTAPVHS 
      GSLGIDTPS DSEEEEIDV VSLEKKPPG GALPTNPST 
      KDRRQIQRT MQSRFPQGL KTIMPVRQP PKKAPRRVK 
      QSRQYKRRL NYSSDSEPE PSEKRHLHN NMERQRRID 
      LRNLFNDLK KLIPDISKK QRAAKVLIL RGAAQYCRD 
      LQSTHEALA RRTEALKQQ QARYRAHLA KLRRDCAAK 
      R
    CDS sequence
    • >TC032473 RA
      atggatgaa tttaaaatc gagttcgac gcgacgtta 
      accgacgac tatctcctg tcggacgac atctggaag 
      cagttccaa atcgaagaa ctgcaaccg ttctcgtcg 
      tcgtttgac tgctcgacg acctgttcc ttcctctgc 
      gatgagaag aactgtcaa gtcaaatac gactgcatg 
      tggaacggc cactgcaaa ggcgcccac ggaaatcga 
      agctgtaat agtaagaca acacctggc gatgtgaag 
      ccacctgtc gtgcaggtg ccccagcag agcctcctc 
      aagccagac ctcaaaata agcgtggat cccaacacc 
      gtggtgccc atgatgccc tccttgcat tactcgatt 
      cagacgccg ccgatttcc gacgatgag gacaccaaa 
      cccacgcaa attctgcag agcttgcaa tcggcactc 
      tctgagaac gagttggac gatgccgat ctgtccgat 
      tatttcaaa gacgacgac gcctggggt ttcttcgac 
      gatggttta gacgaggaa gaggaggag gaggaagag 
      gaagaagat gaaatcgat gaagaacgg agaaaacac 
      gaaaatgac atgagggcc ttctacgcc gccaccgat 
      cacagctac cacaagggg acggcgcca gtgcattcc 
      ggcagtttg ggcatcgac acgccctct gattccgaa 
      gaagaagag atcgatgtg gtgtccctc gagaagaag 
      ccccccggt ggggccctg cccaccaac ccctcgact 
      aaagaccgc cgccagatc cagcgcacg atgcagagc 
      cgcttcccc cagggcctc aagaccatc atgccggtg 
      cgccagccc cccaagaaa gccccccga cgagtcaag 
      cagagccgg cagtacaag cgccgcctg aactacagc 
      agcgacagc gaacccgaa ccgtcggaa aaacgccac 
      ttgcataac aacatggag agacagcgg cgaatcgat 
      ttacggaac cttttcaac gatttgaag aaattgatt 
      cccgatatc agcaagaag cagagagct gccaaagtg 
      ctcattttg cggggggct gcccaatat tgtcgagat 
      ctgcaaagc acccacgag gcgctggcg aggcgaacc 
      gaggcgctg aagcaacag caggcccgg tatagggcg 
      catttggcc aagttgcgg cgagattgc gccgccaaa 
      cgctaa
    mRNA sequence
    • >TC032473 RA
      ggcgtgcaa tgtgctcaa ttcttcctt caagtcagt 
      atttgttgg gccgttcaa gctctcagg cgttcaggt 
      ctctccaag agatatttt tcataagca tgtatacaa 
      agttggtca tagagatta aggagagtg gttttatta 
      acaacgtgt gtctcatat tttcgtcga tcgcttttg 
      tgttacata caccgtgtg tccgaaaaa aactgtaaa 
      aactgtacg agaagtgct ttactgaat acctcgtag 
      ctttaagct gccgtctcg gcgggagcc tcaagtcag 
      ggcaaaatt gtgatatta gtgatagtg acgtaaaat 
      agtaattag ttaaagaaa gtagacgaa aaaaaaaat 
      ccgagaacc catggatga atttaaaat cgagttcga 
      cgcgacgtt aaccgacga ctatctcct gtcggacga 
      catctggaa gcagttcca aatcgaaga actgcaacc 
      gttctcgtc gtcgtttga ctgctcgac gacctgttc 
      cttcctctg cgatgagaa gaactgtca agtcaaata 
      cgactgcat gtggaacgg ccactgcaa aggcgccca 
      cggaaatcg aagctgtaa tagtaagac aacacctgg 
      cgatgtgaa gccacctgt cgtgcaggt gccccagca 
      gagcctcct caagccaga cctcaaaat aagcgtgga 
      tcccaacac cgtggtgcc catgatgcc ctccttgca 
      ttactcgat tcagacgcc gccgatttc cgacgatga 
      ggacaccaa acccacgca aattctgca gagcttgca 
      atcggcact ctctgagaa cgagttgga cgatgccga 
      tctgtccga ttatttcaa agacgacga cgcctgggg 
      tttcttcga cgatggttt agacgagga agaggagga 
      ggaggaaga ggaagaaga tgaaatcga tgaagaacg 
      gagaaaaca cgaaaatga catgagggc cttctacgc 
      cgccaccga tcacagcta ccacaaggg gacggcgcc 
      agtgcattc cggcagttt gggcatcga cacgccctc 
      tgattccga agaagaaga gatcgatgt ggtgtccct 
      cgagaagaa gccccccgg tggggccct gcccaccaa 
      cccctcgac taaagaccg ccgccagat ccagcgcac 
      gatgcagag ccgcttccc ccagggcct caagaccat 
      catgccggt gcgccagcc ccccaagaa agccccccg 
      acgagtcaa gcagagccg gcagtacaa gcgccgcct 
      gaactacag cagcgacag cgaacccga accgtcgga 
      aaaacgcca cttgcataa caacatgga gagacagcg 
      gcgaatcga tttacggaa ccttttcaa cgatttgaa 
      gaaattgat tcccgatat cagcaagaa gcagagagc 
      tgccaaagt gctcatttt gcggggggc tgcccaata 
      ttgtcgaga tctgcaaag cacccacga ggcgctggc 
      gaggcgaac cgaggcgct gaagcaaca gcaggcccg 
      gtatagggc gcatttggc caagttgcg gcgagattg 
      cgccgccaa acgctaact tattcgaat aggtaatag 
      cgcagcccc cttgaagga caggaaact ttgttgcaa 
      cattttcga ggttagcgc aattgtcaa gtgacaaac 
      gtcaactga acagacttg tctgtccgg caagggggc 
      tgtgggtaa tattaactg tgatttatc aacgttgtt 
      ttgtttatt actggcggg ggttttgat tagttgccc 
      cgccaacta ggggtagga tgaattttg tgcttgtta 
      tttttattt acccgatta ggaaatact gttcatttg 
      caataaggc atgcatgtt ttctagttt gtgatgatt 
      tgaatttgt ttgtgatac aatgctctg atctatttg 
      taagaaatc ggtgtttca gttttgttt ttcattttt 
      ccaagagtt gatttagtc gagcaataa tcacacgca 
      aattgctcc tcgattgta cattattta ttttaagtc 
      tttatttta acagttttt atattatta tatgttatg 
      tttgtcgag ggataagga aaatttttt caaggacaa 
      tatttttgt ttgacgggg cccactagt ttccagcct 
      cctcttttt atcgatcaa aatttacga ttctgtggc 
      cattgagtg ccttcctaa tcgaatgac agtggaatt 
      tgtaaaaat tggttaata ggcgaggtt cggttcggt 
      tcacgcgct acatgtttt gtgatagta gatcataag 
      tttgtttag tttcgtggg ctcctgtag aatcaattc 
      acagtgtgt atattatgt gtgtatttt atagcggtt 
      ttttatgtc attgatttg tatatattg tccattttt 
      agtcttcct gaagcgcgt ctgctctga tagtagtcg 
      taagatcaa gagaattgt tgtgttcat gatatttta 
      cagtatttt atacttttg ataaatacg aaattaaac 
      gcaaaaaaa tgagaaaaa aataaaaga aaacaaaaa 
      aattgcttt gcgatgttc ggtttttac tgccatttt 
      ctgcaaagt tcatgtacc tgtgatgta aacctcgct 
      gttgaccaa atatatagt ttaaaaagt ttttcctct 
      ttttcatac aagtgcata gtgacgcag ggcaattct 
      ctaacttat gaccctctt gtggtagcg caaatgttt 
      acaagttat aaccacatt acttttaat ttttcgttt 
      tgttatttg tacttataa tacaataac tacttgtaa 
      tcattggtt gtaatataa ttagtctta tagttttta 
      tcggtggtc gcgaccact attctagtt cctttaagt 
      tacgaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY