| General information | |||
| TC000109 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 11050600..11052505 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000109 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00023 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupation delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (19 dpi)
- >TC000109 RA
MDLFVINRH GDSKPEVTA EEETAKLAT VLKRIERKK QQRIRQRER EREKRQTPK KNPKQKTTV PKRPPKTPE ADEFIEPQP KIKRPPDLK SFTILGAST AAKKPKADR VLPQWLANP TVISSDLQE LTCKVSKLK ELDKGLREQ LKANGVKYL FPVQAEVIP WVLQSRQNA DIMFPRDVC VSAPTGSGK TLAFVLPVV QTLKQFTVR KIRALVILP TQDLAEQVF KSFKLYTQG TRIEVGLVS GKQMFQAEQ KQLVYFNEC FGFVSKIDI LVCTAGRLV NHLKSTNGF NLQALEFLI IDEADRVLE SVQDDWLYH LEKHIYTIQ NRKVLNVCT LQRQRAPQK LLFSATLTQ DPEKIEKLS LFQPKLFTS SVVENSNES EEKPMILTG KYTTPKELT EKYIVCSKD VKPLVLYAF LKRENLTKT LVFTHSVES AHRLKILLK SLFKKRLKI EEISSNLKG KSRDEFISS FTKGEVDLL ICTDFLARG IDLPGVNCV ISYSAPKYL KTYIHRAGR TARAGESGL AVTLLHEEQ VPAFKTLLK KEIEEVEVG VEELEGLSE KYKKALGKL KEAVNEEQK VGMESVLSK KKAFKKLKK KVINSVK
- >TC000109 RA
atggatttg tttgtgata aatcgtcac ggcgactcc aagccggaa gtgacggcc gaggaggaa accgccaaa ctcgccaca gtcctcaaa cgaatcgaa cgcaagaag cagcagcgt atccgtcag cgagagcgc gaacgtgaa aagcgccag accccgaaa aaaaacccg aaacaaaaa accacagtc cccaaacgc ccgcctaaa acccccgaa gcagacgaa ttcatcgaa cctcaaccg aaaattaaa cggccccca gatctgaaa agtttcaca attctgggg gcctcaaca gcggcgaaa aaaccgaaa gctgaccgg gttttgccc cagtggctc gccaacccc actgtgata tcgtcagac ttgcaggaa ttgacctgt aaagtgtcc aagttgaag gagttggat aaggggctc agggagcaa ttgaaggcc aacggggtc aagtatttg tttccggtg caggcggaa gtgatcccg tgggtgttg caatcgcgc caaaatgcc gatatcatg ttcccgagg gacgtgtgc gtgagtgcg cccaccgga agtgggaaa actctcgcg ttcgttttg ccagtggtt cagactttg aaacagttc actgtgagg aaaatcagg gctttggtg attttgccg acgcaagat ttggccgaa caagtgttt aaaagcttc aagttgtac acgcaaggg acgcgaatc gaagtgggc ctggtctca ggcaaacaa atgtttcaa gctgagcaa aaacaattg gtttatttc aacgagtgc tttgggttt gtgagcaaa attgacatt cttgtatgc acggctggt cggttagtg aaccattta aaatcgacc aatggtttt aatttacaa gcgctcgaa tttttgata attgacgaa gctgatcgt gttttggaa agtgttcaa gacgattgg ttgtatcat ttggagaaa cacatttac acgattcaa aacaggaaa gttttaaat gtgtgtact ttacagaga caaagggcg cctcagaaa ctcctcttt tcggcgact ttgacccaa gaccccgaa aaaatcgaa aaattatca cttttccaa ccgaagctt ttcacttca tcggtcgtg gaaaactcc aacgaaagt gaggaaaaa cccatgatt ttgaccgga aaatacaca acaccgaaa gaattaacg gagaaatac atcgtctgt tctaaagac gtcaaacca ctcgttttg tacgcgttc ctcaaacgt gaaaatttg actaaaact ttggttttt actcattca gtcgaaagt gcccacagg cttaaaatc ctccttaaa tcactcttt aaaaaacgg ttaaaaatc gaggaaatt tcctcaaat ttgaagggg aaaagccga gatgaattt atttcaagt ttcacaaaa ggtgaagtt gatttattg atttgtaca gattttttg gctcgaggg attgacttg ccgggggtt aattgcgtg atttcttat tcagccccc aaatatttg aaaacgtac atacacaga gccgggagg acagctagg gcgggtgaa tcggggttg gctgtgacg ctactacat gaggaacaa gtgccggcg tttaaaacc ttgttgaag aaagaaatt gaggaggtt gaggttggg gttgaagaa ttggagggg ttgagtgaa aaatataag aaggcgtta gggaagctc aaggaggcg gttaacgag gagcaaaaa gtggggatg gagagtgtt ttgtccaag aaaaaggct ttcaagaag ctcaagaaa aaggttatt aattcggtt aaatag
- >TC000109 RA
gttgtcaaa aaacgaacg taacctcaa attcgcaca tgtcttgaa cagtaaatt tggcccaaa aaatggatt tgtttgtga taaatcgtc acggcgact ccaagccgg aagtgacgg ccgaggagg aaaccgcca aactcgcca cagtcctca aacgaatcg aacgcaaga agcagcagc gtatccgtc agcgagagc gcgaacgtg aaaagcgcc agaccccga aaaaaaacc cgaaacaaa aaaccacag tccccaaac gcccgccta aaacccccg aagcagacg aattcatcg aacctcaac cgaaaatta aacggcccc cagatctga aaagtttca caattctgg gggcctcaa cagcggcga aaaaaccga aagctgacc gggttttgc cccagtggc tcgccaacc ccactgtga tatcgtcag acttgcagg aattgacct gtaaagtgt ccaagttga aggagttgg ataaggggc tcagggagc aattgaagg ccaacgggg tcaagtatt tgtttccgg tgcaggcgg aagtgatcc cgtgggtgt tgcaatcgc gccaaaatg ccgatatca tgttcccga gggacgtgt gcgtgagtg cgcccaccg gaagtggga aaactctcg cgttcgttt tgccagtgg ttcagactt tgaaacagt tcactgtga ggaaaatca gggctttgg tgattttgc cgacgcaag atttggccg aacaagtgt ttaaaagct tcaagttgt acacgcaag ggacgcgaa tcgaagtgg gcctggtct caggcaaac aaatgtttc aagctgagc aaaaacaat tggtttatt tcaacgagt gctttgggt ttgtgagca aaattgaca ttcttgtat gcacggctg gtcggttag tgaaccatt taaaatcga ccaatggtt ttaatttac aagcgctcg aatttttga taattgacg aagctgatc gtgttttgg aaagtgttc aagacgatt ggttgtatc atttggaga aacacattt acacgattc aaaacagga aagttttaa atgtgtgta ctttacaga gacaaaggg cgcctcaga aactcctct tttcggcga ctttgaccc aagaccccg aaaaaatcg aaaaattat cacttttcc aaccgaagc ttttcactt catcggtcg tggaaaact ccaacgaaa gtgaggaaa aacccatga ttttgaccg gaaaataca caacaccga aagaattaa cggagaaat acatcgtct gttctaaag acgtcaaac cactcgttt tgtacgcgt tcctcaaac gtgaaaatt tgactaaaa ctttggttt ttactcatt cagtcgaaa gtgcccaca ggcttaaaa tcctcctta aatcactct ttaaaaaac ggttaaaaa tcgaggaaa tttcctcaa atttgaagg ggaaaagcc gagatgaat ttatttcaa gtttcacaa aaggtgaag ttgatttat tgatttgta cagattttt tggctcgag ggattgact tgccggggg ttaattgcg tgatttctt attcagccc ccaaatatt tgaaaacgt acatacaca gagccggga ggacagcta gggcgggtg aatcggggt tggctgtga cgctactac atgaggaac aagtgccgg cgtttaaaa ccttgttga agaaagaaa ttgaggagg ttgaggttg gggttgaag aattggagg ggttgagtg aaaaatata agaaggcgt tagggaagc tcaaggagg cggttaacg aggagcaaa aagtgggga tggagagtg ttttgtcca agaaaaagg ctttcaaga agctcaaga aaaaggtta ttaattcgg ttaaatagc aattgtttt gaagtaata aaatatttt ttgtttc
