| General information | |||
| TC000108 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 11052823..11081865 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000108 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00022 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
pupa lethality as pupa - Number of animals showing the phenotype: 8 (19 dpi)
Days post injection
Pupal morphology
wings need term shortened -
Number of animals showing the phenotype: 5
wings proximal blistered - Number of animals showing the phenotype: 5
wings proximal blistered - Number of animals showing the phenotype: 5
- >TC000108 RA
MSDNPPDQP PGQDADVSL NDSECSESV CSDTHSDNT TISEMTEKS TVSLSTIHA TLSNDEEQL HSPSTSADK KSTVAASPA PNRGMLLPW GVHKEYFPQ IISGSSVEL DIRPGEYVM RSLFSDFTI LAEKKIEAV MGEPQEKPL SKTLQRGED PQFDQLLSA FGCVAEHCL PSLLRALYS WYERQMAEV SHHEQKKSD IKQKSIIYM VAGNSTETV ERSEVDIQK ERRDLAVEF IFCLVLIEV LKQLSFHPG HEDLVLYIE NLAFKHFKY REGIQNNPN GSNIHMISD LYAEVIGVL AQSRFQSVK TRFMNELKE LRGREASPH TTQSIISLL MGMKFFRVK MVPIEEFEA SFQYMQECA QYFLEVKDK DVKHALAGL FVEILVPVA AAVKNEVNV PCLKNFVEM LYSQTLDAS TKSKHRLAL FPLVTCLLC VSQKQFFLQ NWHYFLAMC LSHLKNRDP KMCRVALES LYRLLWVYM IRIKCESNT ATQSRLQSI VNSLFPKGS KGVVPRDTP LNIFVKIIQ FIAQERLDF AMREIVFDL LSVGRPIKI ILTPERMSI GLRAFLVVA DSLQQKEGE PPMPRSVGV LPSGNTLRV RKTFLNKML TEDTARSIG ISAYFPHVR RVFVDILRA LDTQYGRPL MMTNTQNVN KEPDEMITG ERKPRIDLF RTCVAAVPR LIPDGMTGS ELVDLLSRL TVHMDEELR GLAYQSLQT LVIDFPDWR QDVLWGLTQ FLAKDVLDT FPNLIDNGL KMILTLLCA WKGALTNTT RIKDTDTMN KRTDLCKSE PMSSVLHLV EALALVMLC NYRVTTKRV SVLILKEVK SLLKVLNCS GNPPVIDII DACCPKVAE KCLPLLPPA EKTAVLTAS CIDLQWLVD RTLSAWNAG LAEDGSIKS GSTYCFNIV DPWSSCLFG FLERERILS QCPNVTAHS WPIVFTRLN ALYSVVDPT PVNDNRASL LRSSATLRK PPNERDAYM HLWKNYITF AFKVVPHMA NPIVRCASP DLSLSSSPD SLSTERSDN KSGGSGTSP TSLYKLVVP LLRCEIADV RDAAVYAVG NINAEACRD LMEELIIYL REAIDKKQE NVRRRRRRD ALRLHLMRT LEYIAESGT FNSSSCILE KDTQSLHPS IVEFVEGVR SYLENEPDK TSSSVKDLT LHFAYFIKK LIKTFSLEA CRGLLKRDL RRNLFMMFS FWAGKYGLT PSNVHETDP AGKKSTELQ FVALQAMSA LLCCGPYFD TTDLAEDSM IYHWLSGLL DSNEEKVNT LAQETVILF LESNPDVGP LLDWVVDKC YTGTYAVAD GCFLALATI FSAREYPCD HYTAIINVT LMNTGCPRP PVRDTALQL LQLLDKRFF GTVGPLAES DLAEGDKGR STLDTLLAT TYRRSQVHL SGQLALLHP ELTMPMFSE ITYRFQSAR PEVRQLLLQ YLLPWLHNM ELVDPNVPP PNPHTYQFY ANETGRGRK EGWGSAEAT EMVLNNIFY ITAKFGDNH SKEIEEVWA TLCTSWPNN MKVIIRYLI IISGMAPCE LLPYTKRVV LYLARVQPV RLLDEMMLE LQTVETLNC LIERTETPP FYRLTVMRK TSSHSDGTN GAGGQSDSN ARQDLNIEK GTIHTKRHS GEDPSKLIS KTNSSNVLS DYNSRMDKL RSSDDGCSS SDDVQGDDM FFREKFEIP IPQPHPLPM PEYGGYFAP LTEYLPDSS QPISGFHRC NVGVMLLCD VVVDGIELD WTVHVPLML HILFLGLDH TRPLVYQHC KQLLLNLLV VLAQHNDHL TVAHILLNS KTNQLQLGL STPSLPILR HSFTEPDSV FDIYLQNGS VVGDAANKI KNCAVLPEG EVSEVKDLT IPPVIVTPE DSPAISICN KLDITVSLV IKSLIDFLS NQAGPLWNY EDITAKVWT VKSAEQIDI FLQHILKIF QFSLPNAHV NERWAQTAL QLGLSCSSR HYAGRSLQI YRALRVPIS SRMLSDILS RLVETVAEQ GEDMQGYVT ELLLTLEAA VESLESDFR PLDFMKDFF KSTPNLNNK ELISHKQIH EGSVAHNAA TALFNHSIQ SGHARSTSY SVSYGLRKN VSSPNETKE RTRNPEIEH RSGNKFSSN LSRSRSAQS LKLLGDSAT QDDKLTILS QLFWLAVSL LESDYEHEF LLAVRLLAR VMHRLPLDR PDARDKVEK LQSQLRWNS FPGVHALLL KGCTHANIY EPVAALLSQ FTPLLNLVV VDPSQTIAF PMNVIALLP YMLLNYEDA NEICIRAAE NIAQVSIEK SKKLENLGT VMNLYSRRT FSKESFQWT KCVIKYLYD TYSHLSFNM LTFLIEVLE KGPNNLQLP ILNIIHCIL HYVDLGSAA AQPINTDLL RVIVKYIEG VHWKEALKI LKLVVTRSS SLVAPPISV HSNWESSLN PVPHPSFNE DSLFARKEL PGRTMEFTF DVSQTPVIG RRFFNKNED GGGANPVVT PRRSCSLSP ADVAPQTGW KRPWMSQGR VRECLVNLL TTCGQRVGL PKSPSVIFS QTSDIIERQ SSMASSTEE MSCGNNDIS GGSRRDDAA TEFGVFKDF DFLEYESES IEGESTDNF NWGVRRRPL SQGEEEQPS LKQLEDSLS EKTPMLTVR KRLMTEESS DDEIGSESP LDEIVIATE FDNSTGEGG VSFPPSSLV LREHPSQTM SSARSDTSG SSAGDLGEV TPCNASPNF SGILSIRPS KSDVLENWT AFIQSNMIQ ISTNTLQFF HQLQNLIRD VCLSTIDIT KDSCCYMMQ SAASGLPTI KLIGSRLNS MIDIMSYRI LPPSLIWFN SSALNNTKF IETLKYGML EVQEHLETF CDKRDQAAV YCNAVKTTL KVEYLSGQH PTEVHHYLL DLGKSLYKL YFQLLLLIE AGNKIIATL HNTLINAEL KDVTVDIIL IKNALLRCT EDNEADGGV SPPTCASSD TNLYKLIES EKFSAAIAF YKANKDELL KELPDVYDA ANDVHAILC LYCKKVIQD KPECFIMTN QEGDLADIF GRLFQVSAA VADLESVIK STTQIEKVT DSKI
- >TC000108 RA
atgtctgat aacccacct gaccagccc cctggccaa gatgcagac gtctccctc aacgattcc gaatgttcc gaaagcgtg tgctccgac acccacagc gacaacacc accatctcc gaaatgacc gaaaaaagc acagtttca ctctcaaca atccacgca acgctatcc aacgatgag gagcagctc cacagcccc tcgacctcc gcagataag aaaagcaca gtggcggcg tccccggct cccaatcgc ggaatgttg ctcccatgg ggggtccac aaggagtat tttccccaa attatttcg gggagtagc gtcgagttg gatataaga ccgggggag tacgttatg aggagtctc ttctccgat tttacgatt ttagctgag aaaaagatc gaggctgtg atgggggaa ccccaggag aagccatta tcgaaaact ttgcaaagg ggggaggat cctcagttt gaccaactg ttgagtgcg tttggatgt gtggctgag cattgtttg ccgtcgctt ttgagggct ttgtattca tggtatgag cgacagatg gcggaggtg agccaccac gagcagaaa aagtccgat attaagcag aaaagtata atttatatg gtggctgga aattcgaca gagactgtt gaacgatca gaagtcgac atacaaaaa gaaagacgt gatttggcc gttgaattt attttttgt ctagtttta atcgaagta ttaaaacag ctgtctttc catcccggc catgaagat ttggtcttg tacatcgaa aatctcgct tttaaacac tttaaatat cgcgagggt attcaaaat aatcccaat ggcagtaac attcacatg atttctgat ctttacgct gaagtgatt ggtgttttg gctcagtcg cgtttccaa tcggtcaaa acccgattt atgaatgaa ttaaaagag ttaagggga cgcgaagcg agtcctcac accactcaa agtattatt agtttactt atgggaatg aaattcttt cgtgttaag atggttccg attgaggag tttgaggca tcgtttcag tatatgcaa gagtgtgca cagtatttt ttggaggtg aaggataag gatgtgaag catgcttta gctggattg tttgtggag attctggta ccagtggcg gcggccgtc aaaaacgaa gtaaacgtc ccatgcctc aaaaacttc gtcgaaatg ctctactcc caaacactg gacgccagc accaaatcc aaacaccgc ctcgccctc ttccctctc gtcacttgc ctactctgc gtaagccaa aaacaattc ttcctccaa aactggcac tatttcctc gcaatgtgt ttatcacac ttgaaaaat cgtgacccg aaaatgtgc cgtgtcgcc ttagaatca ctctaccgc ctcttatgg gtctacatg atccgaatc aagtgtgaa agtaacaca gcgacacaa tcacgcctt cagagcatc gtcaattca ctcttcccc aaaggttcc aaaggggtg gttccccgc gacacccct ttgaacatt ttcgtcaaa attatacaa tttatcgcc caggaacgt ctcgatttc gcaatgcga gaaatcgtt ttcgattta ttatcagtc ggaaggcct attaaaata atcctaacg ccggaacga atgagtatt gggttgcgg gcgtttttg gtggttgcc gatagtttg cagcagaag gagggggaa ccaccgatg cctcgatcg gtcggggtt ttacccagt gggaacacg ctacgcgtt cgcaaaact ttcctgaat aaaatgttg accgaagat acagcacgg agtattggg attagtgcg tacttcccc cacgttcgg cgtgtcttt gtcgatatt ctccgagct ttggatacg caatacgga cgtcccctt atgatgaca aacacgcaa aacgttaac aaagaaccc gacgaaatg ataacaggg gaaaggaag ccccgaatc gatttattc cgaacttgt gtggctgct gttcctcgt ctaattccg gacgggatg actgggtcc gaactggtc gatcttttg agtcgttta accgtccac atggacgaa gaactgcga ggtttggcc taccaaagc ctccaaacc cttgtgatt gatttcccc gattggaga caggatgtt ctatggggg ctcacccaa ttcctggcc aaggatgtg ctagacacc ttccccaat ctcatcgat aatgggctg aaaatgatt ttgacgctt ttgtgtgcc tggaagggg gctctgacc aacaccacc cgaatcaaa gacaccgac acgatgaac aaacgaacc gatttgtgc aaatccgag ccaatgtcg tcggtgctt cacctggtt gaagctttg gcgcttgtg atgttgtgc aattatcgt gtcacgacg aaacgagtc tcggttttg attcttaaa gaagtgaaa agtttgctc aaagttttg aattgttcg gggaatcca ccggtgatt gatataatc gatgcgtgt tgtcccaaa gtggcagag aagtgtctg cctttgttg ccgccggcc gagaaaacg gctgttctt acggcgagt tgtattgac ttgcaatgg ttggttgat cggacgctt tcggcgtgg aatgcggga ttggccgaa gatgggtcg attaagagc gggtcaact tattgtttt aatattgtt gatccgtgg agtagttgt ttgtttggg tttttggaa cgggagagg attttgtcg cagtgtccg aatgtcaca gctcactcg tggccgatt gttttcacg agattgaat gcattgtat tcagttgta gatccaacg ccagttaat gataacaga gcgtcactt ttgaggagt tcggccact ttgcgaaaa ccgccgaat gaacgcgac gcttatatg cacctttgg aaaaattac ataactttt gcgtttaaa gtcgttcca catatggct aatccaatt gtcagatgt gcgagtccg gatctgagt ttgagttct tcccccgat agtttaagt accgaacgc tccgacaac aaatcaggg ggttccggt acttccccg accagtctc tacaagctt gttgttccg cttttgaga tgcgaaatt gccgatgtg agagatgca gcggtttat gccgttggg aacataaat gctgaggct tgtcgagat ttgatggag gagttgatt atttatctt cgcgaagca attgataag aaacaggag aatgtgcgg agacggcgg cgtcgcgac gctttgcgt ctgcatctg atgcgtacg cttgaatat attgccgag agtggtact tttaatagc agttcgtgt attcttgag aaagataca cagtcgctg catccctca attgttgag tttgttgaa ggagtgagg tcgtatctc gaaaatgaa ccggataaa acgtcaagt agcgtcaag gatctgacg ctgcatttt gcctatttc atcaagaaa ttgatcaag acttttagc ctggaagct tgccgcggc ctactgaaa cgcgacttg cgtcgaaat ttattcatg atgttctca ttttgggcc ggaaaatac ggcctaact ccatcaaac gtccacgaa accgatcca gccggtaag aaaagtacc gaattacaa tttgttgca ttacaagcg atgagtgca cttttgtgc tgtggccca tatttcgac accactgat ttggccgag gatagcatg atttatcat tggttgagc ggcttgttg gacagtaat gaagaaaag gtgaatact ttggcacag gaaactgta attcttttc ttggagtcg aaccccgat gttggtcct ttgcttgac tgggtggtt gataagtgt tacacaggg acgtatgca gtggccgat ggttgtttt ctagccttg gcgacgatt ttcagtgct agggaatac ccctgtgac cattatacg gctattatt aacgtcact ttaatgaat acggggtgt ccaaggcct cctgtccga gacacagct ttacagttg ctgcaactg ttggataag agatttttc gggacggtt ggaccactc gccgaaagt gatttagct gagggtgat aagggacgt agcacttta gacacactt cttgcaacg acttatcgc cgatcccaa gtgcattta tcgggtcag ttggcacta ctgcacccc gaattaaca atgccgatg ttttcggaa atcacgtat cggtttcaa tcggcgcgt cctgaagtc agacaattg ctacttcaa tatttactt ccgtggttg cataatatg gaattggtg gaccctaac gtgcctcca cctaaccct cacacgtat cagttttac gccaatgag accggtaga gggcgtaag gaaggttgg gggtcagct gaagccacc gaaatggtt ttaaataat atcttctat attacggcc aaatttggt gataatcat tcgaaagaa attgaggaa gtttgggcc acattgtgt acgtcatgg cctaataac atgaaagtg attatacgt tacttgatt attatatcg gggatggca ccgtgtgaa ttacttcca tacacgaaa cgagttgtt ttatatttg gcaagagtc caaccggtg aggttactc gatgaaatg atgctcgaa ttacaaact gtggaaaca ttgaattgt ttgatcgaa cggactgaa acgccacct ttttaccgc ttgactgta atgcgtaaa acttcaagt cattcggat ggaacgaac ggtgctgga ggacagtcg gactccaac gccagacaa gacttaaac attgagaag ggaactatt catacgaaa cgccacagc ggcgaagac ccgtcgaaa ttaatctcg aaaacgaat tccagcaac gttttgtcg gattataat tcgcgaatg gacaaattg cgaagctca gacgacggt tgttcatca tctgacgac gtccagggc gatgatatg tttttccgt gagaaattc gagattcca atcccacaa ccacaccct ttacccatg ccagaatac ggcggttat tttgcccct ttgaccgaa tatttaccc gacagttcc caaccgatt tcgggattt caccgatgt aacgttggt gtgatgtta ttgtgtgac gttgtggtt gatggaatc gagttggat tggaccgtc catgtgccc ttgatgctt cacatttta tttctgggc ttggaccac accagacct ctggtctac caacactgc aagcaactt ttactcaac ttgctcgtc gtgctcgcg caacacaac gatcaccta accgtggct cacattttg ctcaacagc aaaaccaat caattgcag ttggggctt tcaacgcct agtttacct attttgagg catagtttc accgaaccg gactcggtt tttgatatt tatttacaa aacggttcg gttgttggc gacgctgcg aataaaatt aaaaattgt gccgtttta cccgaaggc gaagttagc gaagttaaa gacttgacc attcctcca gtgattgtc acaccggag gattcaccc gcgatttca atttgtaat aaattggat atcacggtt tcactggtg attaagtca ctgattgat tttttgagt aaccaagcc gggcccttg tggaattac gaagatata acggcgaag gtttggacg gttaaaagt gcagaacaa atcgatatt tttctccag cacattttg aaaattttt caattttcg ttgcctaac gctcatgtt aatgaacgg tgggcccaa acggctcta cagctcggt ttgagttgt tcctcgcga cattacgct ggtcgttct ttacaaatt tatcgcgcg ttgagagta ccgatttcg tcgcgaatg ctttcggat attttgagt cggttggtt gagacggtt gccgaacaa ggggaggat atgcaaggt tacgtaacg gaactgctt ttgactctt gaagcggct gttgagtcg ctagagtca gacttccgc ccgcttgac ttcatgaaa gattttttc aaatctacc ccaaacctc aacaataaa gagttaatc tcgcataag caaatccac gaaggaagt gttgcccac aacgccgcc accgctttg tttaatcat tcgattcaa agtggccat gcacgaagt acgagttat tcggtgtcg tacggtttg agaaaaaat gtttcgtcg cctaacgag accaaagaa cggactcga aatccggaa attgagcac agatcagga aataaattc tcgtcgaat ttgtcacgt tccagatct gctcaaagt ttgaaacta ttgggcgat tcagcgact caagatgat aaattaacc attttatcg cagttgttt tggttggct gtgtcattg cttgagagc gactacgaa catgagttt ctattggct gtgagatta ttggctcgt gttatgcat cggttgcca ctagatcgg cccgatgca agagataaa gtcgagaaa ttgcagtcg cagttgagg tggaattcg tttccgggg gttcacgca ttgttgctg aagggttgt acacatgcg aacatttac gaaccagtg gcggcgctg ttgtcacag tttactcca ctcttaaat ttggttgtg gttgatccg tcacagact atcgctttt ccaatgaac gtcatcgct ttattgccg tatatgttg ttgaattat gaggatgcg aacgaaatt tgtataaga gcagcggaa aatatagca caagtgagc attgagaag agcaaaaaa cttgaaaac ttaggaaca gtaatgaac ctctacagc cgtcgtact ttcagcaaa gagagtttc caatggacg aaatgcgtt atcaaatac ctctacgac acctactcc caccttagc ttcaacatg ctaaccttc ctgatcgaa gtgcttgaa aaaggtccc aataacctc caactcccc atcttaaac atcatccat tgcatcctc cactacgtc gatttaggt tcagcggcc gctcaaccg atcaatacc gaccttctc cgagtcatt gtcaaatac atcgagggg gttcactgg aaggaagct ttaaaaatc ttaaaacta gtcgtaaca cgatcaagt tcactggta gcgccccct atctcagtg cattcaaac tgggagagc agcctcaac ccggtccct catccctcc ttcaacgag gatagttta tttgccagg aaagaacta ccagggcgt acaatggaa tttacattt gacgtttca caaacaccg gtgattggt cgccgattc ttcaataag aatgaggat ggtggggga gccaatccg gtggtgaca cctcgacgt tcgtgttcg ttgagtccg gcagatgtc gctccgcaa accggctgg aaacgacct tggatgtca caggggcgg gttagggag tgtcttgtt aacttattg acgacttgt gggcaaaga gttggactc ccgaaaagc ccatctgtc atattcagt caaacgtcc gatattatc gaacgccag tcgagtatg gcgtccagt actgaagaa atgtcatgt ggtaataac gacatcagt ggcgggtca cgaagggat gacgccgct actgaattt ggtgtcttc aaagatttt gattttctc gaatacgag agtgaaagt attgagggg gaaagtacc gataatttt aattggggc gtccgtcgt aggcctttg agccaaggc gaggaggaa cagccgagt ttgaaacaa ctcgaggat agtttgagt gaaaaaaca cccatgttg accgtcaga aaaagatta atgactgag gaatcatcc gacgatgaa attggttcc gaatcacct cttgacgag attgtgatt gcaaccgaa ttcgataat agcacaggc gaaggtggc gttagtttc cctccatcg tcacttgtt ttacgcgaa catccgtcg cagactatg tcgagtgcg aggtccgac accagtggt tcgagtgcg ggcgatttg ggcgaagtc accccttgc aatgcttcg ccgaatttt tctggaatt ttgtccata aggccgagc aaaagcgac gttttggag aattggacg gcttttatt cagtcaaat atgatacaa atatcgacc aacacgttg cagtttttc caccaatta caaaacctg attagggac gtttgttta tctacaatt gatataacg aaagattcg tgttgttat atgatgcaa tcggccgcc agtggttta cccacgatt aaattaatc ggttctcgc ttaaattca atgattgat attatgtca taccgtatt ttaccaccg agtttgatt tggttcaat tcatcggct ttgaataat acgaaattt atcgaaacg ctgaaatac ggaatgttg gaagtgcaa gaacatttg gagactttt tgtgacaag agggatcaa gccgctgtg tattgcaat gccgtcaaa accactcta aaagtggaa tatttgagt ggccaacat cccactgaa gttcatcat tatttactc gatttggga aaatcgctc tacaagctt tacttccaa ttgttgcta ctgattgaa gctgggaat aaaataatt gcaacgttg cacaacacg ttgataaat gccgaattg aaagatgtg actgttgat ataattttg attaaaaac gcccttttg cgttgcacc gaagacaat gaggcagac gggggcgtt tcgcccccc acttgtgca agttccgac acgaatttg tacaaactg atcgaaagt gagaaattt tcggcggct attgcgttc tataaagcg aataaagac gaattgttg aaagaactt ccagatgtt tacgatgcg gcgaatgat gttcatgca attttgtgt ttgtattgc aagaaagtt attcaggat aagccagag tgttttatt atgacaaat caagagggc gatttagct gatattttt ggacggctg tttcaagtt tcagcagct gtggccgat ttagaaagt gtgattaaa agtacaaca caaattgag aaggtgacc gacagtaaa atttaa
- >TC000108 RA
ccactagaa aatttgtaa tttgacaac tgtgtttga cagtcttgt cagttaaca ataaaaaca gtgaccatt cacaagtaa atgtttacg taaattgtg tttatgtga tgtttatct tgtcccaaa caatgtaaa ctttagtca cgtcacatt tccggttta aaaatcatt gaaaagtga aaattttgg tgtaggtgt aatttgcac aaattcggg tgttaaaag tgcaacgaa ccgtaaatt ataggtttg accatgttg ttgtgaacg aatcgagca atcagcatg tctgataac ccacctgac cagccccct ggccaagat gcagacgtc tccctcaac gattccgaa tgttccgaa agcgtgtgc tccgacacc cacagcgac aacaccacc atctccgaa atgaccgaa aaaagcaca gtttcactc tcaacaatc cacgcaacg ctatccaac gatgaggag cagctccac agcccctcg acctccgca gataagaaa agcacagtg gcggcgtcc ccggctccc aatcgcgga atgttgctc ccatggggg gtccacaag gagtatttt ccccaaatt atttcgggg agtagcgtc gagttggat ataagaccg ggggagtac gttatgagg agtctcttc tccgatttt acgatttta gctgagaaa aagatcgag gctgtgatg ggggaaccc caggagaag ccattatcg aaaactttg caaaggggg gaggatcct cagtttgac caactgttg agtgcgttt ggatgtgtg gctgagcat tgtttgccg tcgcttttg agggctttg tattcatgg tatgagcga cagatggcg gaggtgagc caccacgag cagaaaaag tccgatatt aagcagaaa agtataatt tatatggtg gctggaaat tcgacagag actgttgaa cgatcagaa gtcgacata caaaaagaa agacgtgat ttggccgtt gaatttatt ttttgtcta gttttaatc gaagtatta aaacagctg tctttccat cccggccat gaagatttg gtcttgtac atcgaaaat ctcgctttt aaacacttt aaatatcgc gagggtatt caaaataat cccaatggc agtaacatt cacatgatt tctgatctt tacgctgaa gtgattggt gttttggct cagtcgcgt ttccaatcg gtcaaaacc cgatttatg aatgaatta aaagagtta aggggacgc gaagcgagt cctcacacc actcaaagt attattagt ttacttatg ggaatgaaa ttctttcgt gttaagatg gttccgatt gaggagttt gaggcatcg tttcagtat atgcaagag tgtgcacag tattttttg gaggtgaag gataaggat gtgaagcat gctttagct ggattgttt gtggagatt ctggtacca gtggcggcg gccgtcaaa aacgaagta aacgtccca tgcctcaaa aacttcgtc gaaatgctc tactcccaa acactggac gccagcacc aaatccaaa caccgcctc gccctcttc cctctcgtc acttgccta ctctgcgta agccaaaaa caattcttc ctccaaaac tggcactat ttcctcgca atgtgttta tcacacttg aaaaatcgt gacccgaaa atgtgccgt gtcgcctta gaatcactc taccgcctc ttatgggtc tacatgatc cgaatcaag tgtgaaagt aacacagcg acacaatca cgccttcag agcatcgtc aattcactc ttccccaaa ggttccaaa ggggtggtt ccccgcgac acccctttg aacattttc gtcaaaatt atacaattt atcgcccag gaacgtctc gatttcgca atgcgagaa atcgttttc gatttatta tcagtcgga aggcctatt aaaataatc ctaacgccg gaacgaatg agtattggg ttgcgggcg tttttggtg gttgccgat agtttgcag cagaaggag ggggaacca ccgatgcct cgatcggtc ggggtttta cccagtggg aacacgcta cgcgttcgc aaaactttc ctgaataaa atgttgacc gaagataca gcacggagt attgggatt agtgcgtac ttcccccac gttcggcgt gtctttgtc gatattctc cgagctttg gatacgcaa tacggacgt ccccttatg atgacaaac acgcaaaac gttaacaaa gaacccgac gaaatgata acaggggaa aggaagccc cgaatcgat ttattccga acttgtgtg gctgctgtt cctcgtcta attccggac gggatgact gggtccgaa ctggtcgat cttttgagt cgtttaacc gtccacatg gacgaagaa ctgcgaggt ttggcctac caaagcctc caaaccctt gtgattgat ttccccgat tggagacag gatgttcta tgggggctc acccaattc ctggccaag gatgtgcta gacaccttc cccaatctc atcgataat gggctgaaa atgattttg acgcttttg tgtgcctgg aagggggct ctgaccaac accacccga atcaaagac accgacacg atgaacaaa cgaaccgat ttgtgcaaa tccgagcca atgtcgtcg gtgcttcac ctggttgaa gctttggcg cttgtgatg ttgtgcaat tatcgtgtc acgacgaaa cgagtctcg gttttgatt cttaaagaa gtgaaaagt ttgctcaaa gttttgaat tgttcgggg aatccaccg gtgattgat ataatcgat gcgtgttgt cccaaagtg gcagagaag tgtctgcct ttgttgccg ccggccgag aaaacggct gttcttacg gcgagttgt attgacttg caatggttg gttgatcgg acgctttcg gcgtggaat gcgggattg gccgaagat gggtcgatt aagagcggg tcaacttat tgttttaat attgttgat ccgtggagt agttgtttg tttgggttt ttggaacgg gagaggatt ttgtcgcag tgtccgaat gtcacagct cactcgtgg ccgattgtt ttcacgaga ttgaatgca ttgtattca gttgtagat ccaacgcca gttaatgat aacagagcg tcacttttg aggagttcg gccactttg cgaaaaccg ccgaatgaa cgcgacgct tatatgcac ctttggaaa aattacata acttttgcg tttaaagtc gttccacat atggctaat ccaattgtc agatgtgcg agtccggat ctgagtttg agttcttcc cccgatagt ttaagtacc gaacgctcc gacaacaaa tcagggggt tccggtact tccccgacc agtctctac aagcttgtt gttccgctt ttgagatgc gaaattgcc gatgtgaga gatgcagcg gtttatgcc gttgggaac ataaatgct gaggcttgt cgagatttg atggaggag ttgattatt tatcttcgc gaagcaatt gataagaaa caggagaat gtgcggaga cggcggcgt cgcgacgct ttgcgtctg catctgatg cgtacgctt gaatatatt gccgagagt ggtactttt aatagcagt tcgtgtatt cttgagaaa gatacacag tcgctgcat ccctcaatt gttgagttt gttgaagga gtgaggtcg tatctcgaa aatgaaccg gataaaacg tcaagtagc gtcaaggat ctgacgctg cattttgcc tatttcatc aagaaattg atcaagact tttagcctg gaagcttgc cgcggccta ctgaaacgc gacttgcgt cgaaattta ttcatgatg ttctcattt tgggccgga aaatacggc ctaactcca tcaaacgtc cacgaaacc gatccagcc ggtaagaaa agtaccgaa ttacaattt gttgcatta caagcgatg agtgcactt ttgtgctgt ggcccatat ttcgacacc actgatttg gccgaggat agcatgatt tatcattgg ttgagcggc ttgttggac agtaatgaa gaaaaggtg aatactttg gcacaggaa actgtaatt cttttcttg gagtcgaac cccgatgtt ggtcctttg cttgactgg gtggttgat aagtgttac acagggacg tatgcagtg gccgatggt tgttttcta gccttggcg acgattttc agtgctagg gaatacccc tgtgaccat tatacggct attattaac gtcacttta atgaatacg gggtgtcca aggcctcct gtccgagac acagcttta cagttgctg caactgttg gataagaga tttttcggg acggttgga ccactcgcc gaaagtgat ttagctgag ggtgataag ggacgtagc actttagac acacttctt gcaacgact tatcgccga tcccaagtg catttatcg ggtcagttg gcactactg caccccgaa ttaacaatg ccgatgttt tcggaaatc acgtatcgg tttcaatcg gcgcgtcct gaagtcaga caattgcta cttcaatat ttacttccg tggttgcat aatatggaa ttggtggac cctaacgtg cctccacct aaccctcac acgtatcag ttttacgcc aatgagacc ggtagaggg cgtaaggaa ggttggggg tcagctgaa gccaccgaa atggtttta aataatatc ttctatatt acggccaaa tttggtgat aatcattcg aaagaaatt gaggaagtt tgggccaca ttgtgtacg tcatggcct aataacatg aaagtgatt atacgttac ttgattatt atatcgggg atggcaccg tgtgaatta cttccatac acgaaacga gttgtttta tatttggca agagtccaa ccggtgagg ttactcgat gaaatgatg ctcgaatta caaactgtg gaaacattg aattgtttg atcgaacgg actgaaacg ccacctttt taccgcttg actgtaatg cgtaaaact tcaagtcat tcggatgga acgaacggt gctggagga cagtcggac tccaacgcc agacaagac ttaaacatt gagaaggga actattcat acgaaacgc cacagcggc gaagacccg tcgaaatta atctcgaaa acgaattcc agcaacgtt ttgtcggat tataattcg cgaatggac aaattgcga agctcagac gacggttgt tcatcatct gacgacgtc cagggcgat gatatgttt ttccgtgag aaattcgag attccaatc ccacaacca cacccttta cccatgcca gaatacggc ggttatttt gcccctttg accgaatat ttacccgac agttcccaa ccgatttcg ggatttcac cgatgtaac gttggtgtg atgttattg tgtgacgtt gtggttgat ggaatcgag ttggattgg accgtccat gtgcccttg atgcttcac attttattt ctgggcttg gaccacacc agacctctg gtctaccaa cactgcaag caactttta ctcaacttg ctcgtcgtg ctcgcgcaa cacaacgat cacctaacc gtggctcac attttgctc aacagcaaa accaatcaa ttgcagttg gggctttca acgcctagt ttacctatt ttgaggcat agtttcacc gaaccggac tcggttttt gatatttat ttacaaaac ggttcggtt gttggcgac gctgcgaat aaaattaaa aattgtgcc gttttaccc gaaggcgaa gttagcgaa gttaaagac ttgaccatt cctccagtg attgtcaca ccggaggat tcacccgcg atttcaatt tgtaataaa ttggatatc acggtttca ctggtgatt aagtcactg attgatttt ttgagtaac caagccggg cccttgtgg aattacgaa gatataacg gcgaaggtt tggacggtt aaaagtgca gaacaaatc gatattttt ctccagcac attttgaaa atttttcaa ttttcgttg cctaacgct catgttaat gaacggtgg gcccaaacg gctctacag ctcggtttg agttgttcc tcgcgacat tacgctggt cgttcttta caaatttat cgcgcgttg agagtaccg atttcgtcg cgaatgctt tcggatatt ttgagtcgg ttggttgag acggttgcc gaacaaggg gaggatatg caaggttac gtaacggaa ctgcttttg actcttgaa gcggctgtt gagtcgcta gagtcagac ttccgcccg cttgacttc atgaaagat tttttcaaa tctacccca aacctcaac aataaagag ttaatctcg cataagcaa atccacgaa ggaagtgtt gcccacaac gccgccacc gctttgttt aatcattcg attcaaagt ggccatgca cgaagtacg agttattcg gtgtcgtac ggtttgaga aaaaatgtt tcgtcgcct aacgagacc aaagaacgg actcgaaat ccggaaatt gagcacaga tcaggaaat aaattctcg tcgaatttg tcacgttcc agatctgct caaagtttg aaactattg ggcgattca gcgactcaa gatgataaa ttaaccatt ttatcgcag ttgttttgg ttggctgtg tcattgctt gagagcgac tacgaacat gagtttcta ttggctgtg agattattg gctcgtgtt atgcatcgg ttgccacta gatcggccc gatgcaaga gataaagtc gagaaattg cagtcgcag ttgaggtgg aattcgttt ccgggggtt cacgcattg ttgctgaag ggttgtaca catgcgaac atttacgaa ccagtggcg gcgctgttg tcacagttt actccactc ttaaatttg gttgtggtt gatccgtca cagactatc gcttttcca atgaacgtc atcgcttta ttgccgtat atgttgttg aattatgag gatgcgaac gaaatttgt ataagagca gcggaaaat atagcacaa gtgagcatt gagaagagc aaaaaactt gaaaactta ggaacagta atgaacctc tacagccgt cgtactttc agcaaagag agtttccaa tggacgaaa tgcgttatc aaatacctc tacgacacc tactcccac cttagcttc aacatgcta accttcctg atcgaagtg cttgaaaaa ggtcccaat aacctccaa ctccccatc ttaaacatc atccattgc atcctccac tacgtcgat ttaggttca gcggccgct caaccgatc aataccgac cttctccga gtcattgtc aaatacatc gagggggtt cactggaag gaagcttta aaaatctta aaactagtc gtaacacga tcaagttca ctggtagcg ccccctatc tcagtgcat tcaaactgg gagagcagc ctcaacccg gtccctcat ccctccttc aacgaggat agtttattt gccaggaaa gaactacca gggcgtaca atggaattt acatttgac gtttcacaa acaccggtg attggtcgc cgattcttc aataagaat gaggatggt gggggagcc aatccggtg gtgacacct cgacgttcg tgttcgttg agtccggca gatgtcgct ccgcaaacc ggctggaaa cgaccttgg atgtcacag gggcgggtt agggagtgt cttgttaac ttattgacg acttgtggg caaagagtt ggactcccg aaaagccca tctgtcata ttcagtcaa acgtccgat attatcgaa cgccagtcg agtatggcg tccagtact gaagaaatg tcatgtggt aataacgac atcagtggc gggtcacga agggatgac gccgctact gaatttggt gtcttcaaa gattttgat tttctcgaa tacgagagt gaaagtatt gagggggaa agtaccgat aattttaat tggggcgtc cgtcgtagg cctttgagc caaggcgag gaggaacag ccgagtttg aaacaactc gaggatagt ttgagtgaa aaaacaccc atgttgacc gtcagaaaa agattaatg actgaggaa tcatccgac gatgaaatt ggttccgaa tcacctctt gacgagatt gtgattgca accgaattc gataatagc acaggcgaa ggtggcgtt agtttccct ccatcgtca cttgtttta cgcgaacat ccgtcgcag actatgtcg agtgcgagg tccgacacc agtggttcg agtgcgggc gatttgggc gaagtcacc ccttgcaat gcttcgccg aatttttct ggaattttg tccataagg ccgagcaaa agcgacgtt ttggagaat tggacggct tttattcag tcaaatatg atacaaata tcgaccaac acgttgcag tttttccac caattacaa aacctgatt agggacgtt tgtttatct acaattgat ataacgaaa gattcgtgt tgttatatg atgcaatcg gccgccagt ggtttaccc acgattaaa ttaatcggt tctcgctta aattcaatg attgatatt atgtcatac cgtatttta ccaccgagt ttgatttgg ttcaattca tcggctttg aataatacg aaatttatc gaaacgctg aaatacgga atgttggaa gtgcaagaa catttggag actttttgt gacaagagg gatcaagcc gctgtgtat tgcaatgcc gtcaaaacc actctaaaa gtggaatat ttgagtggc caacatccc actgaagtt catcattat ttactcgat ttgggaaaa tcgctctac aagctttac ttccaattg ttgctactg attgaagct gggaataaa ataattgca acgttgcac aacacgttg ataaatgcc gaattgaaa gatgtgact gttgatata attttgatt aaaaacgcc cttttgcgt tgcaccgaa gacaatgag gcagacggg ggcgtttcg ccccccact tgtgcaagt tccgacacg aatttgtac aaactgatc gaaagtgag aaattttcg gcggctatt gcgttctat aaagcgaat aaagacgaa ttgttgaaa gaacttcca gatgtttac gatgcggcg aatgatgtt catgcaatt ttgtgtttg tattgcaag aaagttatt caggataag ccagagtgt tttattatg acaaatcaa gagggcgat ttagctgat atttttgga cggctgttt caagtttca gcagctgtg gccgattta gaaagtgtg attaaaagt acaacacaa attgagaag gtgaccgac agtaaaatt taatttttt taacgaatg agatttcac tgtgatgtt aaatgtaac acattggag actgaacgt taatttatc agaatttgg gttacaaaa ttaagagta acagcaata caaagaact gacgtcaag tatactatc agtaatcat atttaaaca attgtagtg tggtttagt gtaatttat aataattct attctattc aagattatg atcatccaa ttaattttt tttatatct agcacgaat ttatgattc tatgtacgg aaaataaaa tgcattgaa gcat
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