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Gene information
General information
TC000101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11195909..11207916
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000101
    iBeetle screen iB_00021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000101 RA
      MAAFRYTHA VVCRIPLSL RSRGEIELD EAKKQHETY 
      VRLLRELGL DVIELPPDE ALPECVFVE DTAVVCNGT 
      ALITRPGAP HRTKEVETI RVVLKKELD LPIIEMSDS 
      KAKLDGGDV LFTGREFFV GISQWTNEA GARAVAAAF 
      PEYPCTPIK VPDPKHLKT YITVGGPDL LCVGAGKEA 
      QEVLKRMER EATFSYQTL TLPEDDAAN VLYLNGTLV 
      HRSIDEIPH SFKVFSEKI DYPRRSVNI SQLAKASCG 
      LTSSCILVR RSRHIRNL
    CDS sequence
    • >TC000101 RA
      atggcggct ttccgctac actcatgcc gtcgtgtgc 
      cgcatcccc ttatctctt cgaagtcgc ggtgaaatc 
      gagctcgac gaagccaag aagcagcac gagacctac 
      gtgcgactg ctgcgcgag ctggggctt gacgtcatc 
      gaactgcca cctgatgaa gcccttccg gagtgcgtc 
      ttcgtcgaa gacacggcc gtcgtctgc aacggcacg 
      gcgctcatc accagacct ggagcacct caccgaacc 
      aaagaagtg gaaacaata agggtggtt ctaaaaaaa 
      gagctcgat cttccaata atcgaaatg tccgattcg 
      aaagcgaaa ctggacgga ggtgatgtt ttattcact 
      ggacgggaa tttttcgtg ggcatttca caatggacg 
      aacgaagcg ggggcaagg gctgtggcg gcggctttt 
      ccagagtac ccatgcaca cccatcaag gtgccagac 
      cctaaacac ctcaaaact tacataacg gtcggcgga 
      cccgacttg ctgtgtgta ggggccgga aaagaagca 
      caagaagtg ttgaaacga atggaacgt gaagctact 
      ttcagctac caaacgctg actttacct gaagacgat 
      gcagccaat gttttgtat ttgaatggg actttggtg 
      catcgtagc attgatgaa attccacat tcgtttaaa 
      gtgtttagt gaaaagatt gattatcca cgccgttcg 
      gtgaatatt tcgcagtta gctaaagcc tcctgtgga 
      ttgacttcg agctgtatt ctagtcaga agatcgcgt 
      catataaga aatctctaa
    mRNA sequence
    • >TC000101 RA
      cgttggacg cgacggctg gttccatgt agtcggcgc 
      gtcctggtg ttggaagat ggcggcttt ccgctacac 
      tcatgccgt cgtgtgccg catcccctt atctcttcg 
      aagtcgcgg tgaaatcga gctcgacga agccaagaa 
      gcagcacga gacctacgt gcgactgct gcgcgagct 
      ggggcttga cgtcatcga actgccacc tgatgaagc 
      ccttccgga gtgcgtctt cgtcgaaga cacggccgt 
      cgtctgcaa cggcacggc gctcatcac cagacctgg 
      agcacctca ccgaaccaa agaagtgga aacaataag 
      ggtggttct aaaaaaaga gctcgatct tccaataat 
      cgaaatgtc cgattcgaa agcgaaact ggacggagg 
      tgatgtttt attcactgg acgggaatt tttcgtggg 
      catttcaca atggacgaa cgaagcggg ggcaagggc 
      tgtggcggc ggcttttcc agagtaccc atgcacacc 
      catcaaggt gccagaccc taaacacct caaaactta 
      cataacggt cggcggacc cgacttgct gtgtgtagg 
      ggccggaaa agaagcaca agaagtgtt gaaacgaat 
      ggaacgtga agctacttt cagctacca aacgctgac 
      tttacctga agacgatgc agccaatgt tttgtattt 
      gaatgggac tttggtgca tcgtagcat tgatgaaat 
      tccacattc gtttaaagt gtttagtga aaagattga 
      ttatccacg ccgttcggt gaatatttc gcagttagc 
      taaagcctc ctgtggatt gacttcgag ctgtattct 
      agtcagaag atcgcgtca tataagaaa tctctaaat 
      atgctcact tactacaaa accacctca ggtcttcat 
      taaaagtca ataataatt ttttggatg taatattaa 
      acacggatt tcgtaaagg tgcactagc tttagcttt 
      gctggaatt attattgac ttgtgaaaa taaaaatta 
      actaaaatg gtagcattt atataaaaa cactatgta 
      tgtttttag tgtttcttg ttgatttgt gtttgtttc 
      ctcttgata ttagagatg ttgagcata taaaaattg 
      aaattattt taaagttgt taatactct tgtatcatt 
      ccattgaac gcatttatt gcatcaaat gcagtcgtt 
      tcaaaagca atgtgcatt tatgtgaca atacagaaa 
      ttgagatat ttttgttgc aaatataca agctttacg 
      caaacagtt tgtttcatt ccaatattt ggagtatta 
      tttacgatt acaataaat attgtgctg atttttaat 
      cttaggaat atatttttg tatgtttat ttaaatgga 
      tgtatttat actaacaca actagatga aatgtattg 
      gaaactata aaagaaaaa actgtttga atagttttt 
      tttgaaagt cggttgact catgtattg cttttgtag 
      ttttcaact gtggtgttt tgtacaata aaataggca 
      ttaattaac atccatgtt agatattgt atacatatt 
      taattatcc cgaaatagg tgtcgtcta gttgtggaa 
      ttattgtga ttcagaatc tctctttaa taaagctat 
      tacgaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY