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Gene information
General information
TC000095 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11333952..11335906
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000095
    iBeetle screen iB_00020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000095 RA
      MYQDKIEEL QNYFKSHCK IKEYSGHSS KVHSVGWSC 
      DGKRLASGS FDKSVCIYT LDRDRLNKE ITFKGHGGS 
      VDQLCWHQS HPDLLSTAS GDKSVRIWD TRVQKCVAT 
      INTKGENIN ITWSPNGNA IAVGNKEDL VTFIDARTH 
      KIEAEEQFN FEVNEISWN NTSDLFFLT NGQGCVHIL 
      SYPELKRQH ILKAHPGTC ICIEFDPTG KYFATGSAD 
      ALVSLWDIN ELACQRVFT RMDWPVRTI SFSYDGQLL 
      ASASEDLII DIGFVETGD KIADISVEA ATFTVAWHP 
      SMYFLAYAC DDKDAYDRK RDAGSLKVW GFPSD
    CDS sequence
    • >TC000095 RA
      atgtatcaa gacaaaatt gaggagctc caaaactac 
      tttaagagc cactgtaaa ataaaagag tattcgggg 
      cactcttcc aaagtccat tctgttggt tggagttgc 
      gatggtaaa cgtctggca tctggttct ttcgataaa 
      tccgtctgt atctacact ttggacaga gatcgtttg 
      aataaggaa ataacattc aagggacac ggtggttca 
      gtggaccaa ctctgttgg caccagtct cacccagac 
      ttattaagt acagcaagt ggtgataag tctgttaga 
      atttgggac actcgagtc cagaaatgt gttgccaca 
      atcaataca aaaggggag aatatcaac attacgtgg 
      tcgcctaac ggcaacgcg atcgcagtc gggaacaag 
      gaagacttg gtcactttt atcgacgcc aggacgcac 
      aaaatcgaa gctgaagaa cagttcaat tttgaagtg 
      aacgaaatt tcatggaat aacacaagc gacttgttc 
      tttctaacg aacggacaa ggctgtgtc cacattttg 
      agctatccc gaactgaag aggcaacac attttgaag 
      gcgcatcca gggacttgt atttgtata gagtttgac 
      cctacgggg aaatacttt gcaacgggt agtgccgat 
      gctttggtt tccttgtgg gatattaac gagttggcg 
      tgtcagaga gtgtttaca agaatggat tggcccgtg 
      aggacgatt tcgtttagt tacgatggt cagttgcta 
      gcttcagct tcagaagat ttaatcata gatattgga 
      tttgttgaa actggtgat aagattgcg gatatttcg 
      gtagaagcg gctactttt acggtagct tggcacccc 
      tcaatgtac tttttggcg tacgcttgt gacgataag 
      gatgcatat gataggaaa cgggacgct gggagtttg 
      aaagtttgg gggtttccg tctgactga
    mRNA sequence
    • >TC000095 RA
      tgttggttg tctaaccac cagttttaa ggttatgtt 
      gtgttttgt ttttaccgg tgatttata aatttctca 
      attttaatt atttaaacc attttgcta atacagatt 
      taaataatt acaatggtt tgtgaccac tagagatac 
      gcttttaat aattccaag acctgtatc gaaacatgt 
      atcaagaca aaattgagg agctccaaa actacttta 
      agagccact gtaaaataa aagagtatt cggggcact 
      cttccaaag tccattctg ttggttgga gttgcgatg 
      gtaaacgtc tggcatctg gttctttcg ataaatccg 
      tctgtatct acactttgg acagagatc gtttgaata 
      aggaaataa cattcaagg gacacggtg gttcagtgg 
      accaactct gttggcacc agtctcacc cagacttat 
      taagtacag caagtggtg ataagtctg ttagaattt 
      gggacactc gagtccaga aatgtgttg ccacaatca 
      atacaaaag gggagaata tcaacatta cgtggtcgc 
      ctaacggca acgcgatcg cagtcggga acaaggaag 
      acttggtca cttttatcg acgccagga cgcacaaaa 
      tcgaagctg aagaacagt tcaattttg aagtgaacg 
      aaatttcat ggaataaca caagcgact tgttctttc 
      taacgaacg gacaaggct gtgtccaca ttttgagct 
      atcccgaac tgaagaggc aacacattt tgaaggcgc 
      atccaggga cttgtattt gtatagagt ttgacccta 
      cggggaaat actttgcaa cgggtagtg ccgatgctt 
      tggtttcct tgtgggata ttaacgagt tggcgtgtc 
      agagagtgt ttacaagaa tggattggc ccgtgagga 
      cgatttcgt ttagttacg atggtcagt tgctagctt 
      cagcttcag aagatttaa tcatagata ttggatttg 
      ttgaaactg gtgataaga ttgcggata tttcggtag 
      aagcggcta cttttacgg tagcttggc acccctcaa 
      tgtactttt tggcgtacg cttgtgacg ataaggatg 
      catatgata ggaaacggg acgctggga gtttgaaag 
      tttgggggt ttccgtctg actgaaact atgtgtttc 
      aaaatattc attattttg taatgtgca tttcaaata 
      aattacttc aataaaaat aataggttg tttaggtac 
      aattttcct tgacgaaaa agggtttgc gtcttattt 
      aaggcaaag gtagacgtc gtagtgtgt ttctaagtc 
      aacagatgg tgcggactt tatttttct attatcctc 
      ttatctttt cacatcatt cgaacaaag aatacagag 
      ttgtacata gaatctaaa aaaccttaa taaaatttg 
      caccaacga aatactttt ttatttttt taaatttat 
      ttaaaaaaa gaccataaa taaggcgta agaaaatga 
      ctgaccgaa ttatcggct atgaagagg tggcaatag 
      tatcgaatt ttttaatcc agttcattt aataaatac 
      tgtatccat atattcagt tcttagtag aattttgca 
      acaacgcat ttgcatttt ttttagttt ttacttatt 
      aaatagttt tctgcagcc tactttaca aatctttat 
      tatcttgtc acatttttg ggtaatgac aaagcaaca 
      attaaaaat tactagaaa aatacatcg ctgacacca 
      ctaactaat tcagttcaa aaaagtagt aaaatgctg 
      actgtattt ttctgtaaa tgaagactc gtagatata 
      ggaagcaat taacacacc aacattaaa aaaaataaa 
      cattgctta ttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY