Status
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Gene information
General information
TC000091 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11347575..11352786
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000091
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00019

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000091 RA
      MYHNTSKTG PLVGAIDEG TSSARFILF KAGTTQVVA 
      SHQKELSQI YPQEGWVEQ NPMEILDVV NECIEATID 
      KLIALGGTV NDIVSVGVT NQRESTIVW DKTTGEPLY 
      NSIVWLDVR TSTTVDQLL DKVPNKTRN KNYLKPLCG 
      LPMSPYFSA VKLKWLQDN VPKVKKAMA AKNCLFGTV 
      DSWLIWNLT GGKDGGVHV TDVSNASRT MLMNIDTLK 
      WDPVLLNFF ELPTSILPA IKSSSEVYG KIKEGALQN 
      IPISGCLGD QQAALVGQQ CLDRGQAKA TYGTGCFLL 
      YNTGKAKVD SAHGLITTV AYQLGPSRP ATYALEGSV 
      AVAGAALNW LRDNLTILP TFGEAQSLA EKAELIESG 
      EVYFVPAFS GLYAPYWQQ DARGIIVGI TEDTNSNHI 
      VRATLDAVC FQTRDILEA MNKDYGAPL SHLKVDGGM 
      TANSLLMQL QADLAGIPV MKSSVAEST ALGAAMVAG 
      AAVGCWDIE GAPLDIPAY KWTPRYSEN ERDVRYAKW 
      KMAIERSTG WDI
    CDS sequence
    • >TC000091 RA
      atgtaccac aataccagc aaaacaggc cctttggtg 
      ggcgcgata gatgaggga acctccagt gcaagattc 
      atcctcttc aaagctgga accacccaa gtggtagct 
      tcccaccag aaggaatta tcccagatt taccctcag 
      gaagggtgg gtggagcag aaccccatg gagattttg 
      gacgtggtg aacgagtgt atcgaggct acgattgat 
      aaattgatc gctctgggg ggcaccgtt aacgatatc 
      gtaagtgtg ggggtgacg aatcaaagg gagtcaact 
      atagtatgg gacaagaca actggtgaa cctctttat 
      aactcaata gtttggtta gacgttcgg acgtcgacc 
      accgtcgac caactcctc gacaaagtt ccgaacaaa 
      acaaggaac aagaattat ctgaaaccg ctgtgtggt 
      ctccccatg tcgccttat ttcagtgcc gttaagctc 
      aaatggctc caagacaac gtacccaaa gtgaaaaag 
      gcaatggct gcgaaaaat tgcctattt ggaactgtg 
      gattcgtgg ctgatttgg aacctgaca ggaggcaag 
      gatgggggt gtccatgtc actgatgtc agtaatgct 
      tcaaggacc atgcttatg aacatagac acactaaag 
      tgggatcca gttcttttg aattttttc gaattacca 
      acttccatt ttgccggcg attaagtcc agctcggaa 
      gtgtatggg aaaatcaag gagggcgct ttgcaaaat 
      attccaatt tcagggtgt ctgggtgac caacaggct 
      gctctcgtg gggcaacaa tgtctcgac aggggtcag 
      gctaaggct acttatggc actggctgc ttcctgcta 
      tacaacaca ggcaaagca aaagtagac agcgctcat 
      ggcttgata accactgtg gcttaccaa ttggggcct 
      tcccggcct gccacttac gcccttgag ggctcagtg 
      gccgtggcc ggggctgcc ctaaactgg cttcgagac 
      aacttgaca attttacca actttcggc gaagcccaa 
      agcttagca gaaaaggca gaacttatc gaaagtgga 
      gaagtgtat tttgttcct gctttcagc ggcttgtat 
      gcaccctat tggcaacag gacgctaga ggaataatt 
      gtcggtatt acggaagac acaaactct aatcatatc 
      gtgagggcc actttggac gctgtttgt ttccaaacg 
      agggatatt ttagaggcg atgaataaa gattacggg 
      gcgcctctc tcacattta aaagtggat gggggcatg 
      acggccaat tctcttctg atgcagctc caagccgat 
      ttagcagga atacctgtc atgaagtca agtgtggct 
      gaaagtacg gccctgggg gctgcgatg gtggctggg 
      gctgcagtc ggctgttgg gacattgag ggggcaccg 
      ttggacatc ccagcttat aaatggacc cccagatat 
      agcgagaat gaaagagat gtgaggtac gccaaatgg 
      aagatggcg attgagaga tccacagga tgggatatt 
      tga
    mRNA sequence
    • >TC000091 RA
      agtattttt tgattcgtg tccacattt cctctttta 
      tgtacccac acattaaat gcgttttta tagcgttcc 
      atcgccttt aatacctta ataattgac gctaattac 
      cacagaaca aaggtcaaa aatgttaga taacagatt 
      aaatctagc caaaagtcg tacaatcac acaacttgt 
      gaggttaag atcagttgt tcagaagac aataagccc 
      caaatcatg taccacaat accagcaaa acaggccct 
      ttggtgggc gcgatagat gagggaacc tccagtgca 
      agattcatc ctcttcaaa gctggaacc acccaagtg 
      gtagcttcc caccagaag gaattatcc cagatttac 
      cctcaggaa gggtgggtg gagcagaac cccatggag 
      attttggac gtggtgaac gagtgtatc gaggctacg 
      attgataaa ttgatcgct ctggggggc accgttaac 
      gatatcgta agtgtgggg gtgacgaat caaagggag 
      tcaactata gtatgggac aagacaact ggtgaacct 
      ctttataac tcaatagtt tggttagac gttcggacg 
      tcgaccacc gtcgaccaa ctcctcgac aaagttccg 
      aacaaaaca aggaacaag aattatctg aaaccgctg 
      tgtggtctc cccatgtcg ccttatttc agtgccgtt 
      aagctcaaa tggctccaa gacaacgta cccaaagtg 
      aaaaaggca atggctgcg aaaaattgc ctatttgga 
      actgtggat tcgtggctg atttggaac ctgacagga 
      ggcaaggat gggggtgtc catgtcact gatgtcagt 
      aatgcttca aggaccatg cttatgaac atagacaca 
      ctaaagtgg gatccagtt cttttgaat tttttcgaa 
      ttaccaact tccattttg ccggcgatt aagtccagc 
      tcggaagtg tatgggaaa atcaaggag ggcgctttg 
      caaaatatt ccaatttca gggtgtctg ggtgaccaa 
      caggctgct ctcgtgggg caacaatgt ctcgacagg 
      ggtcaggct aaggctact tatggcact ggctgcttc 
      ctgctatac aacacaggc aaagcaaaa gtagacagc 
      gctcatggc ttgataacc actgtggct taccaattg 
      gggccttcc cggcctgcc acttacgcc cttgagggc 
      tcagtggcc gtggccggg gctgcccta aactggctt 
      cgagacaac ttgacaatt ttaccaact ttcggcgaa 
      gcccaaagc ttagcagaa aaggcagaa cttatcgaa 
      agtggagaa gtgtatttt gttcctgct ttcagcggc 
      ttgtatgca ccctattgg caacaggac gctagagga 
      ataattgtc ggtattacg gaagacaca aactctaat 
      catatcgtg agggccact ttggacgct gtttgtttc 
      caaacgagg gatatttta gaggcgatg aataaagat 
      tacggggcg cctctctca catttaaaa gtggatggg 
      ggcatgacg gccaattct cttctgatg cagctccaa 
      gccgattta gcaggaata cctgtcatg aagtcaagt 
      gtggctgaa agtacggcc ctgggggct gcgatggtg 
      gctggggct gcagtcggc tgttgggac attgagggg 
      gcaccgttg gacatccca gcttataaa tggaccccc 
      agatatagc gagaatgaa agagatgtg aggtacgcc 
      aaatggaag atggcgatt gagagatcc acaggatgg 
      gatatttga agtgttata atggttata atcagtttt 
      cgatgacat atttttata tctattgga gggctgcgt 
      gttatgtta ctttagatt tttcagaga taagacttt 
      gttacataa aaaatcaat ttgttgttt tatttcttc 
      aaagtacta acactccgc actaaaatt agtaccgca 
      tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY