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Gene information
General information
TC000085 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 6957641..6965183
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000085
    iBeetle screen iB_00016
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs sometimes everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen dorsoventral-axis shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 0 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000085 RA
      MENSNSEGV VLPDISEAE QLASEHDGG ENGDSSVDV 
      KQARVSAVS VRHAGVPVS LPVGSLITS TFNVITQDQ 
      LPHFKPMLC VDNNGYIAT DGSGAELKT IVIQGDVSE 
      AGAVSTPVT VSGPNLGSW NEAASLPVL PVRCKNTSA 
      ELHKNRFGS GGRGRCIKL GSSWYTPSE FEALCGRAS 
      SKDWKRSIR FGGRSLQTL IDEGIILPH ATSCTCAAC 
      CDDESATGP VRLFTPYKR KRRKDHDPN KRSCGEDSD 
      GNQQSKEEA WQNLAEGLD STDYQLMEP VVNSDPTAS 
      LKRLEDIAS QITRLASDF RKGVEELRD AISRQSEKF 
      QREKDAAVL AARVEAQVA ALQPDGSSD TTLQPALDD 
      NQKKCANCN REALAECSL CRRTPYCST FCQRKDWVS 
      HQVECVRGV TTDANSQQS IMLIVESGE QQ
    CDS sequence
    • >TC000085 RA
      atggagaac tcaaactcg gaaggcgtc gtcctaccg 
      gacatttcc gaagccgaa cagctcgcg agcgagcac 
      gatggcggc gagaatggc gactcctcc gtggacgtg 
      aagcaggcg cgcgtctcc gccgtctcg gtgcgccat 
      gcgggcgtc ccggtgtcc ctgccggtg ggttcgctc 
      atcaccagc accttcaac gtcatcacc caggaccag 
      ctgccccac ttcaagccg atgctgtgc gtcgacaac 
      aacggctac atcgccacc gacggctcg ggggccgag 
      ctcaagacc atcgtcatc cagggcgac gtctcggag 
      gcgggggcc gtctccacg ccggtgacg gtgtccggg 
      cccaacctg ggctcgtgg aacgaggcg gcgagtttg 
      ccggtgctg ccggtcagg tgcaagaac acctcggcg 
      gagctgcac aagaacagg ttcggctcg gggggcaga 
      ggccgctgc atcaagttg ggctccagt tggtacacg 
      ccgagcgag ttcgaggcg ctctgcgga agggcctcc 
      agtaaggac tggaagagg agtattagg ttcggcggg 
      aggagtctg caaacgttg attgatgaa ggtattatc 
      ctccctcat gcaacaagt tgcacttgc gccgcttgc 
      tgcgatgac gaatcggca acaggtccc gtccgcctg 
      ttcacccct tacaaaaga aagcgtcga aaggatcac 
      gacccgaac aagcgcagc tgtggcgaa gacagtgac 
      ggcaatcag caatcgaaa gaggaagca tggcaaaac 
      ctggccgaa ggcttagac tccaccgat tatcagtta 
      atggaaccg gtggtcaat tcagatccc acagcttcc 
      ttgaaacgg ctcgaagac atcgcgtcg caaataacg 
      cgactagcg agcgatttc cgcaaaggt gttgaagaa 
      ttacgtgac gccattagc agacaatcg gagaaattt 
      caaagagaa aaggatgct gcagttttg gcggcgcga 
      gtggaggcc caagtggcg gcgcttcag cctgatggc 
      agctcggat acgaccctg caaccggct ttggacgat 
      aaccagaaa aagtgtgcg aattgcaac agggaggca 
      ctggccgaa tgttccctc tgtcggcgg acgccttac 
      tgttccact ttctgtcaa cgtaaggat tgggtctcg 
      catcaagtc gagtgtgtt aggggtgtg acaacggac 
      gctaattca caacagtca attatgctg attgttgaa 
      agcggagag caacaataa
    mRNA sequence
    • >TC000085 RA
      cctttcact ttacatatc caccaccaa tcaccttca 
      aggataaac agtaacaaa tttgcagct tgaacctga 
      atttctttg tttttgaag ctgattttg attgaattc 
      tttcacgga atgtcgtaa aatgggtta attgtcata 
      attaaaatt cgcttacat tgtgaaaaa cggagcgta 
      aaattcaaa aatggcgcg gcgcatgcg cagcccctt 
      gcacgccgc ctgcatatc ggcaagtgt agtaaccaa 
      ccgtgcatc cgtcatcgt catcacccc aagtggcgt 
      catgtaaat ccgttaaac tcctccacc tcttcgaga 
      ccgcttcct ctgaaaaac ccttggtaa acgtcgtcg 
      tgtccgcgt tttcgtcca tggagaact caaactcgg 
      aaggcgtcg tcctaccgg acatttccg aagccgaac 
      agctcgcga gcgagcacg atggcggcg agaatggcg 
      actcctccg tggacgtga agcaggcgc gcgtctccg 
      ccgtctcgg tgcgccatg cgggcgtcc cggtgtccc 
      tgccggtgg gttcgctca tcaccagca ccttcaacg 
      tcatcaccc aggaccagc tgccccact tcaagccga 
      tgctgtgcg tcgacaaca acggctaca tcgccaccg 
      acggctcgg gggccgagc tcaagacca tcgtcatcc 
      agggcgacg tctcggagg cgggggccg tctccacgc 
      cggtgacgg tgtccgggc ccaacctgg gctcgtgga 
      acgaggcgg cgagtttgc cggtgctgc cggtcaggt 
      gcaagaaca cctcggcgg agctgcaca agaacaggt 
      tcggctcgg ggggcagag gccgctgca tcaagttgg 
      gctccagtt ggtacacgc cgagcgagt tcgaggcgc 
      tctgcggaa gggcctcca gtaaggact ggaagagga 
      gtattaggt tcggcggga ggagtctgc aaacgttga 
      ttgatgaag gtattatcc tccctcatg caacaagtt 
      gcacttgcg ccgcttgct gcgatgacg aatcggcaa 
      caggtcccg tccgcctgt tcacccctt acaaaagaa 
      agcgtcgaa aggatcacg acccgaaca agcgcagct 
      gtggcgaag acagtgacg gcaatcagc aatcgaaag 
      aggaagcat ggcaaaacc tggccgaag gcttagact 
      ccaccgatt atcagttaa tggaaccgg tggtcaatt 
      cagatccca cagcttcct tgaaacggc tcgaagaca 
      tcgcgtcgc aaataacgc gactagcga gcgatttcc 
      gcaaaggtg ttgaagaat tacgtgacg ccattagca 
      gacaatcgg agaaatttc aaagagaaa aggatgctg 
      cagttttgg cggcgcgag tggaggccc aagtggcgg 
      cgcttcagc ctgatggca gctcggata cgaccctgc 
      aaccggctt tggacgata accagaaaa agtgtgcga 
      attgcaaca gggaggcac tggccgaat gttccctct 
      gtcggcgga cgccttact gttccactt tctgtcaac 
      gtaaggatt gggtctcgc atcaagtcg agtgtgtta 
      ggggtgtga caacggacg ctaattcac aacagtcaa 
      ttatgctga ttgttgaaa gcggagagc aacaataac 
      cggttttat gactttgtg ctatgtttg aaatagccc 
      tacttttta gtgtttttt ttctgatca gaaaagtga 
      ctggtgagt ttggagcta agttggcaa cttaacttc 
      aaagtcatg gccacttgg accagaaat ttaattgac 
      agcatgata tttatagta tcttgccca cacatatct 
      gtctgcgtt tgtggattc ccgtgggat ttttatcaa 
      tttctatca tgatggtgt atatattgt acatatgtt 
      tcattttgt aaattttga acggctcgt cgcattagg 
      actaaattg tgctacttt atttaacaa ttaaagcat 
      ttttaacgt t