Status
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Gene information
General information
TC000078 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11336038..11344065
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000078
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00014

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupa lethality as pupa - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    Pupal morphology
    wings blistered - Number of animals showing the phenotype: 9

    iBeetle screen iB_09772

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000078 RA
      MATLRPKSP RPVGPKNDK EESIWDKLG TLGRKKRIK 
      EVQEVQAEG KYAIDSPGH PTNPITPPE EYDLEDNEE 
      RCMVEPRSY DDPKFRELS QVLIDWIND ELASQRIIV 
      QDLAEDLYD GQVLQKLLE KLTNDKLDV PEVTQSEEG 
      QKQKLSVVL AHFNQVLGV QRLGHKKWS VEAIHSKNI 
      VPILHLLVA LARHFRPPV RLPEHVSVT VVVVQKVGG 
      QLSHRIVKE SITTAYDDV GMRCERDAF DTLFDHAPD 
      KLQVVKKSL VTFVNKHLN KINLEVSDL ETQFHDGVY 
      LIMLMGLLE GFFVPLYSF HPTPRDFEQ KVHNVSFAF 
      ELMEEVGLK RPKARPEDV VNSDLKSTL RVLYNLFTK 
      YKNIM
    CDS sequence
    • >TC000078 RA
      atggcgaca cttcggcct aaatcaccc aggcctgtg 
      gggcccaaa aacgataag gaggagtcg atctgggac 
      aagcttggt acacttggg cgcaagaaa cgcatcaag 
      gaagtgcaa gaggtgcaa gctgagggc aaatatgcg 
      attgattct ccggggcat cccacaaac cccatcaca 
      ccgccagaa gagtacgat ttggaggac aatgaggag 
      cgctgcatg gtggagccc cggtcgtac gacgacccc 
      aaattcagg gaactctcc caagtactg atcgactgg 
      atcaatgac gaattggcc tcacagcga atcattgtg 
      caagaccta gctgaggac ttgtatgat gggcaagtg 
      ttgcaaaaa cttctcgaa aagctaaca aatgacaaa 
      ctcgacgtt cccgaagtc acccagtct gaagaaggc 
      caaaagcag aaactttcg gtcgttttg gcacatttc 
      aaccaggtt ttgggggtg cagcgtttg gggcacaag 
      aaatggagt gtggaggct attcattcg aaaaatatc 
      gtcccaatt ttgcattta ttggtcgct ttggcgcga 
      catttcaga ccaccagtt cgtcttcct gaacatgtc 
      tctgtcaca gttgtggtg gtccagaaa gtggggggc 
      caattgagt cacagaatt gttaaagaa agcattacc 
      actgcctat gatgatgtc gggatgagg tgtgagagg 
      gatgcgttt gatacattg tttgatcat gcccccgat 
      aaactccaa gttgtaaaa aagagtctc gtaacattc 
      gtcaacaaa cacttgaac aaaatcaat ttggaagtg 
      agtgatctt gaaactcaa ttccatgat ggggtctat 
      ttaattatg ttgatgggt cttttggag ggatttttt 
      gtacctttg tacagcttc catcctact ccaagggat 
      tttgagcaa aaagttcac aacgtctcg ttcgctttt 
      gaactcatg gaagaagtc gggttgaaa agaccaaaa 
      gcacgacct gaagatgtt gtcaactct gatctcaaa 
      tctaccttg agagttctt tacaatctc tttacgaaa 
      tataaaaat attatgtag
    mRNA sequence
    • >TC000078 RA
      atttcaaaa atatgcggt ttccgcctt atcaaacgt 
      ttcaaacac tattctaat aaaacagtg ttgaattaa 
      ttgttttta aacacaaga agatggaag aatttatag 
      aaaagccac cttgccaaa ttttatacc gacaatctt 
      cgttggaaa ctcgcttat ctcccgttc acttgtctg 
      aagaatcat ccgaatcaa gtgatgagg aagtggaaa 
      atgttaaca ccacaccca aaatagtgg acgttaaaa 
      taaaaacac cagtagaaa aaggcaacg aaaacgagg 
      aggaaaaat taacagcgg tatatttaa tgaaaagta 
      agttcacaa cttgataac attaaatac aaattattt 
      aatacgcaa ctaatcaaa gtttttaaa acaaaacag 
      tgcaccatt aattaaaac ttaatcact ttgcataat 
      tgaattttc gacatctgc taaaaccca ctggtggtc 
      aacaatctc caaagcctc ccattccgc tttaaaggg 
      cactttggg gtcgtttgg catcgccat ggcagcccc 
      cgacatttg atcctgcat ttgcttgct ctgatcggc 
      cgcgttatt ctcccccaa aaccagcgc gtaaatgct 
      cctcaagcc aaacacgtt taaaaagta ccacgaagc 
      tgacgaaac ccacgacgc gtccaaatg cgacaattc 
      gatccctct ttgcagcat cgacttaaa ccgcagagt 
      taggggaaa cggcacctt tgtcgtaat aaaacccga 
      aaacatcca attaatcca ccccccgat aataatcat 
      gggcaagac attggtcaa attgccttt caacatatc 
      cgtcatcat tgaggggtc ggtcatcat gttttgcgt 
      cacgggggg gcgtttctg catcaaata gccctcctc 
      ttccctgtt aaaggcatg ttttcggat caggaacga 
      cgtcttcgg tatatactt ggcgttttc gcgcaaaac 
      ccgcgccct gaggatcaa ctggctgtc ttgcaactg 
      ttggatttc taccttttt ctggcttga cagaaggat 
      ggagatgta gtgccgcaa gatgccgac caggaatgt 
      gatcaccac gatgggcaa aaacaccca gaaacgtat 
      ttgtgggtc gacaattaa atcggtggt catggttag 
      gtttctgac acttgtctg actgacact agtgaattt 
      caggcatca caccaccaa aacaacaaa ctcccccag 
      agaaatgtc aaagtgagt tagaaatgg cgacacttc 
      ggcctaaat cacccaggc ctgtggggc ccaaaaacg 
      ataaggagg agtcgatct gggacaagc ttggtacac 
      ttgggcgca agaaacgca tcaaggaag tgcaagagg 
      tgcaagctg agggcaaat atgcgattg attctccgg 
      ggcatccca caaacccca tcacaccgc cagaagagt 
      acgatttgg aggacaatg aggagcgct gcatggtgg 
      agccccggt cgtacgacg accccaaat tcagggaac 
      tctcccaag tactgatcg actggatca atgacgaat 
      tggcctcac agcgaatca ttgtgcaag acctagctg 
      aggacttgt atgatgggc aagtgttgc aaaaacttc 
      tcgaaaagc taacaaatg acaaactcg acgttcccg 
      aagtcaccc agtctgaag aaggccaaa agcagaaac 
      tttcggtcg ttttggcac atttcaacc aggttttgg 
      gggtgcagc gtttggggc acaagaaat ggagtgtgg 
      aggctattc attcgaaaa atatcgtcc caattttgc 
      atttattgg tcgctttgg cgcgacatt tcagaccac 
      cagttcgtc ttcctgaac atgtctctg tcacagttg 
      tggtggtcc agaaagtgg ggggccaat tgagtcaca 
      gaattgtta aagaaagca ttaccactg cctatgatg 
      atgtcggga tgaggtgtg agagggatg cgtttgata 
      cattgtttg atcatgccc ccgataaac tccaagttg 
      taaaaaaga gtctcgtaa cattcgtca acaaacact 
      tgaacaaaa tcaatttgg aagtgagtg atcttgaaa 
      ctcaattcc atgatgggg tctatttaa ttatgttga 
      tgggtcttt tggagggat tttttgtac ctttgtaca 
      gcttccatc ctactccaa gggattttg agcaaaaag 
      ttcacaacg tctcgttcg cttttgaac tcatggaag 
      aagtcgggt tgaaaagac caaaagcac gacctgaag 
      atgttgtca actctgatc tcaaatcta ccttgagag 
      ttctttaca atctcttta cgaaatata aaaatatta 
      tgtaggttt tcagggaag acaatagtg ccttggaag 
      tgtctttgc cagatctaa atgtgcttt atcgtgcct 
      tagacagaa atatgtatt tttaacacg attgattat 
      atttatcca agtgttgta ggaacccac taatgtaaa 
      taac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY