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Gene information
General information
TC000021 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16166029..16221003
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000021
    iBeetle screen iB_00002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000021 RA
      MRSHKKTKE SGFFEGDSS DTDGCFNMG KRTCTTSSD 
      CPKKIKYKD EDYDNPIRT PQHDKVNLS GVDRVNMSH 
      FDLLKVLGT GAYGKVFLV RKRGGADNN RLYAMKVLK 
      KATIVQKKK TTEHTKTER QVLEAVRDN PFLVTLHYA 
      FQTDAKLHL ILDYVAGGE LFTHLYQRE HFTEDEVRI 
      YIGEIILAL ERLHSLGII YRDIKLENI LVDESGHIV 
      LTDFGLSKE LPREGDNDQ RAYSFCGTI EYMAPEVVK 
      GGTQGHDIA VDWWSVGVL TYELLTGAS PFTVEGEKN 
      TQQEISRRI LKTTPPIPD SLGKDVADF ISKLLVKDP 
      RKRLGGGEG DAKELKKHS FFKSLDWEK LARKEIPAP 
      FKPVIRSEL DVSNFSEEF TQMPPTDSP AVVPPNYDK 
      IFKGYSYVA PSVLFTKNA ISDEILKPA KQHDSTKLI 
      SCKINNSQF FQTYDIDLN EPILGDGTF SVCRKCVNL 
      QTGKEYAVK IVSRKKDCS QEINLLRAC QGHPNIVTL 
      HDVIQDEAH TYLVLEYLK GGELFERIR KKSKFTESE 
      ASGILRKLV SAVSFMHSC GVVHRDLKP ENLVFTDDD 
      DSAEIKVID FGFARLKQE KESLHTPCF TLHYAAPEV 
      LKGDPEGYD ENCDLWSLG VILYTMLCG KAPFHARSR 
      DENVSSIME RIKEGDFNF NSSAWTGVS NEAKSVVRG 
      LLTVNPGQR LRMSHLETN PWVQGSQHF VSTPLMTPD 
      VLLETAPEK SLQTTFNAF HKAQREGFR LQDVFSAKL 
      AQRRRMKKS SSGDNSTSS SSFTSEGSL KSGPKLSVD 
      VKKTDKVAL GGNVFNFGE NRVTEFLET MSSNPLDPE 
      TVSPCREST SSSGIVMSD KNSVDTKVV GRKRQKKVY 
      VQTRWSERI RRRQRQEID LDPVPVKHN RKRKVDERE 
      SIESTSSSS KRKKTMAPL RKSKKSKIG R
    CDS sequence
    • >TC000021 RA
      atgcggtcc cataaaaaa accaaagaa agtggcttt 
      ttcgaaggt gattcctcg gacacagat ggttgtttt 
      aacatgggt aaaaggaca tgtactaca tctagtgat 
      tgtcctaaa aagattaag tacaaggac gaggactac 
      gacaaccca attaggaca ccacagcac gacaaagtt 
      aatctgtca ggagtggac cgagtcaat atgtcccac 
      ttcgatctg ctcaaagtt cttggaaca ggagcgtac 
      ggtaaagta ttcctagtg cgaaaacgc ggcggtgcc 
      gacaacaat cgtctctat gccatgaaa gtactgaaa 
      aaggcgacg atagtgcag aagaagaaa acgacggag 
      cacacaaag accgaacgc caagtccta gaagccgtc 
      cgagacaat ccgttttta gttacactg cactacgcc 
      ttccaaacg gatgccaaa ttacaccta atcctcgac 
      tatgtggcc ggaggagaa ctattcaca catttatac 
      caaagagag cattttaca gaagatgaa gtccgcatt 
      tacataggg gaaattatt ttagcactc gaacgcctc 
      cacagtttg ggtatcata tacagggac atcaagcta 
      gaaaatatc ttagtcgac gaatcggga cacatcgtc 
      ctcactgat ttcggattg agcaaagaa ctacctagg 
      gaaggtgac aatgaccag cgggcgtac tctttttgt 
      ggcacaatc gagtatatg gcacccgaa gtggtcaag 
      ggaggcacc caaggacac gatattgcc gttgactgg 
      tggtccgtg ggcgtactg acttacgaa ttattgact 
      ggagcttcc cctttcacc gtcgagggt gaaaagaac 
      actcaacag gaaatctcc agacgaatc ctaaaaact 
      actccgccg atacctgat tctttagga aaagatgta 
      gccgatttt attagtaaa ttattagtc aaggatcca 
      agaaagcga ttaggtggt ggcgaagga gacgccaaa 
      gaactgaaa aagcactca ttcttcaaa agtctggac 
      tgggagaag ttggccagg aaggagatc ccggctcct 
      ttcaaacca gttattagg agcgaactg gacgtgtca 
      aacttcagc gaagaattc acccaaatg cctccaacc 
      gactcacca gccgtcgtt cctccaaac tacgataaa 
      atcttcaag ggctattca tacgttgcg ccctctgtc 
      ctcttcacc aaaaacgcc atctcggac gagatcctc 
      aaaccggcc aaacagcac gatagtaca aaactgatc 
      agttgcaaa atcaacaat tcgcaattc ttccaaacg 
      tacgacatc gacctaaac gagccaatt ttaggcgat 
      ggcaccttt tcagtatgt cgtaaatgt gtcaactta 
      caaacgggt aaagaatac gctgtgaaa atcgtcagc 
      aggaaaaaa gattgctca caagagatt aatctattg 
      cgcgcctgt caaggccac ccaaacatc gtcaccctc 
      cacgatgtt atccaagac gaagcgcac acctacctg 
      gtcttggag tacctcaaa ggtggcgaa ctgttcgaa 
      cgcattcgg aaaaaatcg aaattcacc gaatcggaa 
      gcgtcgggg attttacgc aagttggta tcggctgtg 
      agtttcatg cattcgtgt ggtgtcgtc catcgggac 
      cttaaaccg gagaatttg gtttttacc gatgatgac 
      gacagtgct gagattaag gtgattgat tttggcttt 
      gcgaggctc aagcaggag aaggagtcg ttgcatacg 
      ccgtgtttt acccttcat tacgccgcc cctgaggtg 
      ctaaagggt gacccggaa gggtacgac gaaaactgt 
      gatttgtgg agtttgggg gtgattctc tacacgatg 
      ctgtgcggg aaggcacct ttccatgca cgttcacgg 
      gacgaaaac gtctcttcc atcatggag cgcatcaaa 
      gagggcgat tttaatttc aattcgtcg gcttggacc 
      ggtgttagc aacgaggcc aagtcggtg gtgaggggg 
      ctcctgacc gtgaacccg ggccaaagg ctgcgcatg 
      agccacctg gagaccaac ccctgggtg caagggagc 
      caacatttc gtctcaacc cccttgatg acgcccgat 
      gtgctgcta gaaaccgcc cccgagaag agtctccag 
      accacgttc aacgccttc cacaaggcg cagcgtgag 
      ggcttccgg ctccaggac gtgttcagt gccaagctg 
      gcgcagagg cgaaggatg aagaagagc tcctccggt 
      gacaacagc acctcctcg tcgagtttc acgagtgaa 
      ggctctcta aaaagcgga ccaaagcta tctgttgac 
      gtcaaaaag actgataaa gtcgcactc ggaggcaac 
      gttttcaac tttggggag aaccgagtg acggagttt 
      ttggagacg atgagttcg aatcccctg gacccggaa 
      acggtgtct ccgtgccgg gagtcgacg agttccagt 
      ggcatcgtc atgtcggat aagaattcg gtggatacg 
      aaggtggtg gggaggaag aggcagaag aaggtttac 
      gtgcagacg aggtggagc gaacgcatt cgcaggaga 
      cagcgccag gagattgac ctcgatccg gttcctgtg 
      aaacacaac aggaagcgg aaggttgac gaaagagag 
      agtatagag tcgacatcg tcgagttcg aagaggaaa 
      aagacgatg gcgccactt aggaaatca aaaaaaagc 
      aaaatcggt cgatag
    mRNA sequence
    • >TC000021 RA
      ttagaaaaa acatctgga actatttgc taaagaagg 
      cgtttttta acagtttcg tggttttga gttttgaga 
      tctagagat tagcattat gcattagca taacacctt 
      ctgattttt ttgttgttt gttgtttct gtacctctc 
      accttttgg caatcaatt ctatagcaa aataatttt 
      aaaatattt agtaacttt attataact tctttggct 
      tcctgtagt accattcgt atttgtcat tttgtaaaa 
      tacataaca ctgtagtca catccttat tttttataa 
      actcaccat tccatattt tatctgctt ccggattaa 
      actgaatgg aactaagca aagttcata gaaatatct 
      ttttagcaa tttgtgaaa ctgtaaata tcatgcatg 
      caatgcata ctcatacaa ttaagtcat tcttcagcg 
      tcctttgat tttgaaata aagttaagt tggcggtac 
      tgaataata aaaacactg tcagttcag caccgctgt 
      caacttgtg taactttaa aatcacttt gttaaaaaa 
      ttaacggtt gttatttta taaataatt aatatgtta 
      gtataatat ttactgaaa gagatatgt ggcgacgcc 
      gagcgtcga gttattaaa ttttgcgac tcgtcgctt 
      gctccaaaa catgcgctc ttttcttct ttcgcccaa 
      gataaaaat aaaatagtc aataaataa ccaaatgct 
      tgattaaac gtatttcca atgaaattc aaggatgat 
      gtaattaaa aatgcgata atattgcag aagatattc 
      acgttcttc aatgtttgt atcatttca atgccacct 
      ctgatctca gcaattaca gaaatatcc gcacatgag 
      atggtttga atgaattat atttttagc attgaaacc 
      tcaaatatt tcctcacac caagcgaat tccccaaat 
      atttacaca gatatttcc aacgaaagg tggagacgc 
      ggaaaaaat aggtaggat ttatctatg aagtgcgtt 
      ggtggaaca acttgtaca atatttcag ttgtgaaag 
      gaaaagttt gtttgaatg aagtgtaag cggacttgg 
      actagccta ctgcgattg cgattccga gagtagcag 
      tcccaaaac atgggctaa aatcggcaa ataccgagt 
      gattgaagt aataaatta atccaaaat aattaaacg 
      agtatttgt tcacataaa ataaaccga tccaagcta 
      aaaaaaatg gcgtaaaat cagtttcaa tcgcacgat 
      aaatataaa gtgaattta tttacatct gggtcagtg 
      ggtgtttgt ctttagcaa caggtggtg gttgtgatg 
      gcgatggag tcgtctgtg caaaaacaa gacaatgcg 
      acattttcc aaaacgcca aagcccctg tcgagatca 
      cccggaacc tcaccgtct atccggaac atgacacag 
      accgtcatc gtatcactg gctgagact gtgacacac 
      gatatcaca tacggtaag tacagaacc gcatcttgc 
      tagcaagaa tgtaaatat ttatgcgtg ggtggtgtt 
      ttgttaaga cattcaaaa atcaaagtg tggtttcta 
      actacagtt atcttatcg cagccaact tgttattta 
      atcaccttg aatttattt ttaaaacag ttccgatgt 
      ctcgagtat ttcagtaca gatgatttt gtttgcatt 
      gtgaggcat ttttgcgat acaatgaat attgaaatt 
      ttaaatctt caagcgcgc cttttaaaa ccacattgt 
      ggtcacgtt cctatcaaa tgaatgtgc gttcggcgc 
      ctctgattg gtggggata tggaaacaa catggaatc 
      gacaactgt ctaaaatca gttactttg tagacgcgt 
      ttgtcggtt gtattaagt tgtattttt gaatttctc 
      aattatttc ctacctcat gttcagtta actaagcct 
      gaaaccttc ttcctagca cgcaaaacc cgcccaaac 
      aaagtgaat ttcgagaat gtgttgaag ttcgaaaac 
      ctaacctga caaactgtc tatctgaaa gtttgtgat 
      agtgattgg cgacaagtt taaagagtt tcctgtgag 
      aatccgaaa aggcacgcc acggactgt gaaacggta 
      caagtgaat caaatttac tgttacgac ctagttttg 
      tgatactac cagcacaag tcttcggct caagtcagc 
      tcgttattc ggggaataa ctcaatttt ttaaatatg 
      tacttggtg taattgtac gcaatcagg caaaatgcg 
      gtcccataa aaaaaccaa agaaagtgg ctttttcga 
      aggtgattc ctcggacac agatggttg ttttaacat 
      gggtaaaag gacatgtac tacatctag tgattgtcc 
      taaaaagat taagtacaa ggacgagga ctacgacaa 
      cccaattag gacaccaca gcacgacaa agttaatct 
      gtcaggagt ggaccgagt caatatgtc ccacttcga 
      tctgctcaa agttcttgg aacaggagc gtacggtaa 
      agtattcct agtgcgaaa acgcggcgg tgccgacaa 
      caatcgtct ctatgccat gaaagtact gaaaaaggc 
      gacgatagt gcagaagaa gaaaacgac ggagcacac 
      aaagaccga acgccaagt cctagaagc cgtccgaga 
      caatccgtt tttagttac actgcacta cgccttcca 
      aacggatgc caaattaca cctaatcct cgactatgt 
      ggccggagg agaactatt cacacattt ataccaaag 
      agagcattt tacagaaga tgaagtccg catttacat 
      aggggaaat tattttagc actcgaacg cctccacag 
      tttgggtat catatacag ggacatcaa gctagaaaa 
      tatcttagt cgacgaatc gggacacat cgtcctcac 
      tgatttcgg attgagcaa agaactacc tagggaagg 
      tgacaatga ccagcgggc gtactcttt ttgtggcac 
      aatcgagta tatggcacc cgaagtggt caagggagg 
      cacccaagg acacgatat tgccgttga ctggtggtc 
      cgtgggcgt actgactta cgaattatt gactggagc 
      ttccccttt caccgtcga gggtgaaaa gaacactca 
      acaggaaat ctccagacg aatcctaaa aactactcc 
      gccgatacc tgattcttt aggaaaaga tgtagccga 
      ttttattag taaattatt agtcaagga tccaagaaa 
      gcgattagg tggtggcga aggagacgc caaagaact 
      gaaaaagca ctcattctt caaaagtct ggactggga 
      gaagttggc caggaagga gatcccggc tcctttcaa 
      accagttat taggagcga actggacgt gtcaaactt 
      cagcgaaga attcaccca aatgcctcc aaccgactc 
      accagccgt cgttcctcc aaactacga taaaatctt 
      caagggcta ttcatacgt tgcgccctc tgtcctctt 
      caccaaaaa cgccatctc ggacgagat cctcaaacc 
      ggccaaaca gcacgatag tacaaaact gatcagttg 
      caaaatcaa caattcgca attcttcca aacgtacga 
      catcgacct aaacgagcc aattttagg cgatggcac 
      cttttcagt atgtcgtaa atgtgtcaa cttacaaac 
      gggtaaaga atacgctgt gaaaatcgt cagcaggaa 
      aaaagattg ctcacaaga gattaatct attgcgcgc 
      ctgtcaagg ccacccaaa catcgtcac cctccacga 
      tgttatcca agacgaagc gcacaccta cctggtctt 
      ggagtacct caaaggtgg cgaactgtt cgaacgcat 
      tcggaaaaa atcgaaatt caccgaatc ggaagcgtc 
      ggggatttt acgcaagtt ggtatcggc tgtgagttt 
      catgcattc gtgtggtgt cgtccatcg ggaccttaa 
      accggagaa tttggtttt taccgatga tgacgacag 
      tgctgagat taaggtgat tgattttgg ctttgcgag 
      gctcaagca ggagaagga gtcgttgca tacgccgtg 
      ttttaccct tcattacgc cgcccctga ggtgctaaa 
      gggtgaccc ggaagggta cgacgaaaa ctgtgattt 
      gtggagttt gggggtgat tctctacac gatgctgtg 
      cgggaaggc acctttcca tgcacgttc acgggacga 
      aaacgtctc ttccatcat ggagcgcat caaagaggg 
      cgattttaa tttcaattc gtcggcttg gaccggtgt 
      tagcaacga ggccaagtc ggtggtgag ggggctcct 
      gaccgtgaa cccgggcca aaggctgcg catgagcca 
      cctggagac caacccctg ggtgcaagg gagccaaca 
      tttcgtctc aaccccctt gatgacgcc cgatgtgct 
      gctagaaac cgcccccga gaagagtct ccagaccac 
      gttcaacgc cttccacaa ggcgcagcg tgagggctt 
      ccggctcca ggacgtgtt cagtgccaa gctggcgca 
      gaggcgaag gatgaagaa gagctcctc cggtgacaa 
      cagcacctc ctcgtcgag tttcacgag tgaaggctc 
      tctaaaaag cggaccaaa gctatctgt tgacgtcaa 
      aaagactga taaagtcgc actcggagg caacgtttt 
      caactttgg ggagaaccg agtgacgga gtttttgga 
      gacgatgag ttcgaatcc cctggaccc ggaaacggt 
      gtctccgtg ccgggagtc gacgagttc cagtggcat 
      cgtcatgtc ggataagaa ttcggtgga tacgaaggt 
      ggtggggag gaagaggca gaagaaggt ttacgtgca 
      gacgaggtg gagcgaacg cattcgcag gagacagcg 
      ccaggagat tgacctcga tccggttcc tgtgaaaca 
      caacaggaa gcggaaggt tgacgaaag agagagtat 
      agagtcgac atcgtcgag ttcgaagag gaaaaagac 
      gatggcgcc acttaggaa atcaaaaaa aagcaaaat 
      cggtcgata ggtaattat tcaattcgt taggtgtga 
      tctttcaaa agacattaa cggtggaac caaagctcg 
      gccctctcg agggaagta tttgtgtac atagtgcgg 
      tggtttatg caataattg gggcaggcg gggctttgg 
      ctcaccgct attgcgatt gtgataaaa tgttaacct 
      taaaatgct gatgaattc gattgatga tcacattta 
      gtgttaagt tccatttag gtatatcat tatcattaa 
      gtgcacttc ttaggctga atcttaatt cattaaaat 
      accaaaaag ttgtaaata aaattatat agttttttt 
      gtaagttac tttgtgttt tatacaagt ctcatctaa 
      agaatttta ttagagaaa cggttgcaa ttaatattg 
      atgtttgga acggaatcg cttaaaata atgttgatt 
      atgtatttt tagagcctc tgaatttta cctgcgagt 
      aaaaaacga atgtttgtt gtgtctggt gttgatatc 
      ttttatcat tatttattt tcattctat ttattcttc 
      ctgttacct aactatgaa acacttgtt taaagttta 
      ttgttattg ttatgtata atatgcaac acttcaagc 
      tgaatggat catagtgaa aataatgtg tttctagca 
      ttcaattac ctggtctat gttttagtc aatattgtt 
      agttatggg ttacaatgt aagttgatc caattgatt 
      atttataat tttgcactc aggtaccgt tcttattat 
      tattattac aaatcacta atttgtagt attttcatt 
      acttattta tagaagatt tgtttgtat atttgtttc 
      aaatttatc tcaatattt tttaaataa tttcttttg 
      cataagaac tacttatga ttatgtaaa gcaaagtgt 
      atattcata cgacatgat ggagaccaa gtggacagt 
      gcctttcct ctttcaaga ggctgtcat gcttgaagg 
      caaaattta tattactgt aacatgcct taattatgt 
      tagtattaa atattttga ttcttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY