| General information | |||
| TC034414
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12330071..12343025 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034414 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01898 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 25.0% (includes death as larva, prepupa, pupa, adult)
- >TC034414 RA
MNGTAIDDG STIDRRPLI TCAGELEVF STIEDNLSQ ALPQDSCEW RRSLGRPVR SVHIGANFA PFSAAALPK GAQWDLIRY PLFHIYWTE CTDVDLYKN TVRDDIENW LKELQTRDI PDFLVVVVE NYDGKRTNK LLPRTTVLD KIRTDFAPK QGDRCISVI NPGKVEARS ADSWRGLVS RIRHLLLVA YARAVSRLE DHVRQQRER RNEIGWNFM QYFHLQEEL AHVLEMLGV HEEALVQYD ELDALFSQF VVNGITGES INWLGDFQK PLEQWHGLK INHSTMLSE SPSLLELRA YLFAKQAQM LLKANKVWE MASRCLPFL HLTTREITV LEINSPPGA VSCWLFLAC VEVLQVCDK YNNADAVED YSLHTACLW AYASEKLRD LGELCGLMP GGAPTSEQL HTVVGLSAG MGDNPGAPD SPSPTDKLK EALCSQEAF KKTYLELAE LAMCTYKHI GRLRSARLV GREVASFYL LLGETQTAA AFLSDAVRT FEQDKWHEL AAQTQIELA ECYRKANDT KKFIKTSAS VAAALEIDT LIRWSYFDE MRKCLQSLE HPLVVPFND IIKITSVAL KNEAIILQD STINVELVI ESNFPREIL CTNVQLSIE LDTKEGRKS EKYSKSRVL TGKDLKPRD PLLQRLQIF RHLDLKQDK TLASASVVC KHPEHKRKD SAAAPPKSD FQCCLQVAN LPLVVTPGV NVIRLSKQA TNVGKHYLG QMAIFMKNL HLISQLLTP RVAFEVKEE APVVRLHKT DAPLLSGFE QIMNLIITI GSYTVEPKS RIKLTASRG LSFQTDPSE PLTTSLEIE LGERPPFSS TRTPLRVLA DLLTKDHQV SISCPWTSK PIVVKLSFT PPLTTIWRL NTVQHRKFV QINVTGQCE KDIYVEKPQ LSVGDFCKI VDRNHSSSH CAFRLPESD TDFCATLRS AKPCRDAPP HHSSGAPPR TSSPVDSIA FNSGASLRA EAEGGMNYS ATRDAADSC WTKLYKRGE LRAAGSWNF SRRQTAENY SKQVIKSPD IWTPIEIIH KWLCFKPLQ AEKML
- >TC034414 RA
atgaatggt acagcaatt gacgacggg tcaacgatt gacaggcga ccactaatt acatgcgct ggcgagttg gaagtcttt tcaacgatt gaagacaac ttgtctcaa gccctgccg caggattcg tgcgagtgg cggcggtcg ttgggtcgc cctgtgcgc tcggtgcac attggggcc aatttcgcg ccctttagt gctgcggcc ctgcccaag ggggcgcag tgggatctg atcaggtat cctttgttc cacatttac tggacggag tgcacggac gtggatttg tacaaaaat acagtcagg gatgatatt gagaattgg ttgaaggag ttgcagacg cgggacatt cccgatttt ttggtcgtt gtggtggaa aattacgat ggaaaacgt actaataag ctgttaccg aggaccact gttttggat aagatccgg acagatttt gctcccaaa caaggggat cggtgcata tctgtgata aacccaggc aaagttgag gcccgttct gctgactcc tggcggggt ttagtgagt cgcattcgc cacttgttg ttggtggcg tatgcgcgt gctgtatct cgccttgag gaccatgtg aggcaacaa cgcgaacgt aggaacgaa attggttgg aattttatg caatatttt cacctccaa gaggaattg gctcacgtt ttagaaatg ttaggcgta cacgaggaa gctttagtg caatacgac gaattagat gctctcttt tctcaattt gtagtaaac ggaatcaca ggcgagtcg ataaactgg cttggcgat ttccaaaag cctctagaa cagtggcat ggacttaaa atcaatcac tcaacaatg ctctctgaa agtccgtct ctgttagag ttgagagcg tatttgttt gcaaagcag gctcaaatg ttgcttaaa gcaaataaa gtgtgggag atggcttca aggtgtttg ccttttctt catttaact acaagggaa ataacagtt ttagaaata aattcgccg cctggtgcg gtctcttgt tggttgttt ttggcctgt gttgaggtt ttacaggtt tgtgacaag tacaataat gccgatgct gttgaagac tattccttg catacggcc tgtttgtgg gcttatgca agtgaaaag ttgcgcgat ttgggcgaa ttgtgcggc ctaatgcct ggtggggcc cccacctct gagcaacta cacaccgtg gtggggcta tcagctgga atgggcgat aatcccggg gccccagac agcccttca cccacggac aagctcaaa gaagcgtta tgttcgcag gaggctttc aaaaaaaca tatttggag ttggcagaa ctggcaatg tgcacgtac aaacacatc ggccgtctc cgctcggcc cgacttgtg ggccgcgaa gtggcctcg ttttacctc cttttaggg gaaacccaa acagctgcg gcattttta agcgacgca gttcgcact ttcgaacag gacaaatgg cacgaattg gccgctcaa acccaaatc gaactcgcc gaatgttac cgaaaggcc aacgatacg aaaaaattc attaaaacg agtgccagt gtggccgca gccttggaa atcgatact ctgatccgt tggagctat tttgacgaa atgagaaag tgtctccaa tcactcgaa catccccta gtagtgcct ttcaacgat attatcaaa attacgagt gttgcccta aagaacgaa gcgattatt ctgcaagac agcaccatc aatgtggaa cttgtgatc gaatcgaac tttccgcgg gagattctg tgcactaat gtgcaactg tcgatcgag ttggacacg aaagagggc cgaaaaagc gaaaagtac agcaaaagt cgcgtgttg actgggaaa gacttgaaa ccgcgcgat cccttatta caaagactc caaattttt cgacatttg gacctgaag caggacaaa acgttggcc agtgcgagt gtggtttgt aagcatcca gagcacaag aggaaggat agcgcggca gcgccgcct aagagcgat tttcagtgt tgtctccaa gtcgccaat cttccgctt gttgtcaca cccggtgtc aatgtgata agactgagt aaacaggcg acgaacgtt gggaagcac tacttgggc caaatggcc atttttatg aagaatctt cacttgatc tcgcaattg ttgacgccg agagtggct ttcgaagtg aaggaggag gcgcctgtt gtgaggctg cacaagacc gatgctccg ttgttatca gggtttgag caaattatg aatttgatt attacgatc gggagctat acagttgaa ccaaagtct agaataaaa ctaactgct tctcggggt ttaagtttc caaacagac cccagcgag cccctaacc acaagttta gaaatcgaa ctcggggaa agaccccct tttagtagt actcgaaca ccgttacga gtactcgcc gatcttcta acaaaagat caccaagta tccattagt tgtccttgg acgtctaaa ccaattgtt gttaagttg agctttaca ccgcctttg acgacaatc tggcgatta aatacagta caacatcgg aaatttgtc caaatcaat gtgacagga caatgcgaa aaagacata tacgtagaa aaaccccaa ctgagcgtg ggcgacttt tgtaagatt gttgataga aatcatagt tctagtcat tgtgcgttc cggttaccg gagtcggac acggatttc tgcgctacg ctccggagt gcgaaaccg tgccgagac gctcctccc caccattca tccggagcc ccaccacgg acttcgtca cctgttgac agtattgca ttcaactcc ggggcgtct ctgcgagcg gaagccgag ggtggcatg aattattca gcgacgagg gacgctgcc gattcctgc tggacaaag ttgtacaaa cgaggggaa ttacgcgcc gccggaagc tggaatttt tcgcgccga caaacggcc gaaaactat tctaaacaa gtaattaaa agtccagat atttggacg ccgattgaa attattcac aagtggctg tgtttcaag ccactgcag gcggaaaag atgctttaa
- >TC034414 RA
cgtgaaatg acaacacgt tttaatttt tggggtgtt taatcatct gtcaaactc atattgatg gtatttatt ttagttgtg tgtgttaaa atcattaat gaaatacaa tacgaacca tgaagatat caccccgcc cgccccttt aaccgaaaa accacgcaa tagagtatc ttccgacaa tgacctttt ttttgcctc ctggttaag tttcaaatt ccaatcatg aatggtaca gcaattgac gacgggtca acgattgac aggcgacca ctaattaca tgcgctggc gagttggaa gtcttttca acgattgaa gacaacttg tctcaagcc ctgccgcag gattcgtgc gagtggcgg cggtcgttg ggtcgccct gtgcgctcg gtgcacatt ggggccaat ttcgcgccc tttagtgct gcggccctg cccaagggg gcgcagtgg gatctgatc aggtatcct ttgttccac atttactgg acggagtgc acggacgtg gatttgtac aaaaataca gtcagggat gatattgag aattggttg aaggagttg cagacgcgg gacattccc gattttttg gtcgttgtg gtggaaaat tacgatgga aaacgtact aataagctg ttaccgagg accactgtt ttggataag atccggaca gattttgct cccaaacaa ggggatcgg tgcatatct gtgataaac ccaggcaaa gttgaggcc cgttctgct gactcctgg cggggttta gtgagtcgc attcgccac ttgttgttg gtggcgtat gcgcgtgct gtatctcgc cttgaggac catgtgagg caacaacgc gaacgtagg aacgaaatt ggttggaat tttatgcaa tattttcac ctccaagag gaattggct cacgtttta gaaatgtta ggcgtacac gaggaagct ttagtgcaa tacgacgaa ttagatgct ctcttttct caatttgta gtaaacgga atcacaggc gagtcgata aactggctt ggcgatttc caaaagcct ctagaacag tggcatgga cttaaaatc aatcactca acaatgctc tctgaaagt ccgtctctg ttagagttg agagcgtat ttgtttgca aagcaggct caaatgttg cttaaagca aataaagtg tgggagatg gcttcaagg tgtttgcct tttcttcat ttaactaca agggaaata acagtttta gaaataaat tcgccgcct ggtgcggtc tcttgttgg ttgtttttg gcctgtgtt gaggtttta caggtttgt gacaagtac aataatgcc gatgctgtt gaagactat tccttgcat acggcctgt ttgtgggct tatgcaagt gaaaagttg cgcgatttg ggcgaattg tgcggccta atgcctggt ggggccccc acctctgag caactacac accgtggtg gggctatca gctggaatg ggcgataat cccggggcc ccagacagc ccttcaccc acggacaag ctcaaagaa gcgttatgt tcgcaggag gctttcaaa aaaacatat ttggagttg gcagaactg gcaatgtgc acgtacaaa cacatcggc cgtctccgc tcggcccga cttgtgggc cgcgaagtg gcctcgttt tacctcctt ttaggggaa acccaaaca gctgcggca tttttaagc gacgcagtt cgcactttc gaacaggac aaatggcac gaattggcc gctcaaacc caaatcgaa ctcgccgaa tgttaccga aaggccaac gatacgaaa aaattcatt aaaacgagt gccagtgtg gccgcagcc ttggaaatc gatactctg atccgttgg agctatttt gacgaaatg agaaagtgt ctccaatca ctcgaacat cccctagta gtgcctttc aacgatatt atcaaaatt acgagtgtt gccctaaag aacgaagcg attattctg caagacagc accatcaat gtggaactt gtgatcgaa tcgaacttt ccgcgggag attctgtgc actaatgtg caactgtcg atcgagttg gacacgaaa gagggccga aaaagcgaa aagtacagc aaaagtcgc gtgttgact gggaaagac ttgaaaccg cgcgatccc ttattacaa agactccaa atttttcga catttggac ctgaagcag gacaaaacg ttggccagt gcgagtgtg gtttgtaag catccagag cacaagagg aaggatagc gcggcagcg ccgcctaag agcgatttt cagtgttgt ctccaagtc gccaatctt ccgcttgtt gtcacaccc ggtgtcaat gtgataaga ctgagtaaa caggcgacg aacgttggg aagcactac ttgggccaa atggccatt tttatgaag aatcttcac ttgatctcg caattgttg acgccgaga gtggctttc gaagtgaag gaggaggcg cctgttgtg aggctgcac aagaccgat gctccgttg ttatcaggg tttgagcaa attatgaat ttgattatt acgatcggg agctataca gttgaacca aagtctaga ataaaacta actgcttct cggggttta agtttccaa acagacccc agcgagccc ctaaccaca agtttagaa atcgaactc ggggaaaga ccccctttt agtagtact cgaacaccg ttacgagta ctcgccgat cttctaaca aaagatcac caagtatcc attagttgt ccttggacg tctaaacca attgttgtt aagttgagc tttacaccg cctttgacg acaatctgg cgattaaat acagtacaa catcggaaa tttgtccaa atcaatgtg acaggacaa tgcgaaaaa gacatatac gtagaaaaa ccccaactg agcgtgggc gacttttgt aagattgtt gatagaaat catagttct agtcattgt gcgttccgg ttaccggag tcggacacg gatttctgc gctacgctc cggagtgcg aaaccgtgc cgagacgct cctccccac cattcatcc ggagcccca ccacggact tcgtcacct gttgacagt attgcattc aactccggg gcgtctctg cgagcggaa gccgagggt ggcatgaat tattcagcg acgagggac gctgccgat tcctgctgg acaaagttg tacaaacga ggggaatta cgcgccgcc ggaagctgg aatttttcg cgccgacaa acggccgaa aactattct aaacaagta attaaaagt ccagatatt tggacgccg attgaaatt attcacaag tggctgtgt ttcaagcca ctgcaggcg gaaaagatg ctttaatta aaagttggg acatgaaaa agttcgctt tgaacgact ttgcaaact tttaagtcg caaaaattc ataatcgag actaaacaa gccaaatat tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
