Status
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Gene information
General information
TC033626
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009695,TC009696 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4627279..4631692
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033626
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033626 RA
      MKRVFFSYQ FLATVTSSL AVLSSGIHE AWTSVYIPV 
LLNGTNTLK ITSVEGSWI TMSMSFGGL IGCVVSCLL 
INKIGRKKT ILLTFCPNF LSSVVLAFA NSVPVFCTA 
RVLSGVAFG IAIGVIPHY IGEIADPEI RGSLGTLVT 
IFSLSGFLF INIVGSYVT IQTSSWISA TIPVLFLLT 
FIWMPESPY YLVMIGECD QAEQTLAKL KGTRDVFDE 
FQNLKDVMP VSVITIALT FGLNVWHSF IMSDPTVTI 
QQGQLRGKI STNIDNKNF YSFQGIPYA KPPTGPLRF 
KDPQAPEPW TGVKDATTE GSVCYAKHM LFNNIIGSE 
DCLHLNVYT PELASKNLR PVMVWIHGG AFKSGSSNT 
DVYGPEYLL TQDVVVVTF NYRLGIFGF LKLDDATLG 
VPGNAGMKD MVMALKWVR KNISKFGGD PNNVTIFGE 
SAGAASVHY LVLSPLAKG LFHRAIAQS GTALNSFAR 
GRSDMVSHI SAELNTKNE REILEKLVQ MPAEQLFEL 
YEKLVTVCE GLNNAGGKL PFAPVIEKA SQNAFIAEE 
PLKIIKSGN YNQVPLIFG FTTREGMLI EQMIKPRKP 
TMPRDFEAA IPYTLEVER GSENSKQIA QKIKDFYYG 
EPGSEDKLE NFYIIHTDN YFLRDIIFA VKHHAATSK 
FPVYLYQMS VDSSLNVMK RLSNITAPG VAHGDDIGY 
LFKTKVSPA LNPGSLEEV SVRRFVRLW TNFARNGNP 
DEPQWKPVK TNELNYIDI GEKLSAKVN PEPERMKFW 
EELYKNKPL YSI
    CDS sequence
    • >TC033626 RA
      atgaagcgt gtgtttttt tcgtaccag tttttggcc 
actgtgacc tcctcgttg gctgtttta tcgtcgggg 
attcacgaa gcctggact tcggtctac atccccgtt 
ttgctgaac ggcaccaac actttgaaa ataacaagt 
gtggaaggg tcctggatt acgatgtcc atgagtttt 
ggcggattg atcggctgt gttgtgtct tgccttctg 
attaataaa atcggacgc aaaaaaacg atactcctg 
acgttttgt cccaatttt ttgtcctcg gtggtcctg 
gcttttgcc aattcggtg ccggttttt tgcacagct 
cgggtttta tcgggtgtt gctttcggg attgcaatc 
ggggttatt ccccattat atcggcgaa atcgcagat 
ccggaaatt aggggttcg ttgggcaca cttgtgact 
attttttcg ctgtctggt ttcctcttt ataaacatt 
gtcggttcg tacgtaacg atacaaaca tcgtcttgg 
atttcggca acaattcca gttctattc ctactgact 
tttatttgg atgccggaa agtccgtac tatttggta 
atgattgga gagtgcgat caggctgaa caaactttg 
gcaaaactt aagggaaca cgcgatgtt tttgacgag 
ttccaaaat ttgaaagat gtcatgcca gtttctgtt 
atcactatt gccctaacc ttcggttta aacgtctgg 
cacagtttc atcatgtct gatcccacc gtcaccata 
cagcaggga caactccgg ggaaaaatc tcgaccaac 
attgacaat aaaaacttc tacagtttc caaggcatt 
ccatacgcc aaacccccc actggacca ctccggttc 
aaggaccct caagccccg gaaccatgg accggagtc 
aaggacgca acgacggaa ggaagcgtg tgttacgcc 
aaacacatg cttttcaat aacattatc ggatcggag 
gattgccta cacttgaat gtttacaca ccggaattg 
gcatcaaaa aatttaaga ccagtcatg gtctggata 
catggtggc gcctttaaa agcggctca agtaatacg 
gatgtctac ggtcctgag tatttgcta actcaggat 
gttgttgtc gttactttc aactacaga ttgggaatt 
ttcgggttt cttaaactg gatgatgcc acgttaggt 
gttcccggg aacgcggga atgaaagac atggtaatg 
gccttgaaa tgggtccgg aaaaacatc agtaaattc 
ggtggggat ccgaacaat gtgaccatt tttggtgaa 
agtgctgga gcagcctcg gtgcattac ctagtcttg 
tcaccacta gccaaaggg ctgtttcac agggccatt 
gcacagagt gggactgcg ttaaattct tttgcaagg 
gggcgcagt gacatggtt tcgcatatc tcggctgag 
ttgaacacc aaaaatgag agggaaatt ttggaaaag 
ttggtgcaa atgccagcc gaacaactg tttgaactg 
tacgagaaa ttagtaact gtgtgtgag ggtttgaac 
aatgccggt ggtaaacta cccttcgct ccggtgatt 
gaaaaggcg tctcaaaat gcgttcatt gccgaagaa 
ccgctcaaa atcataaaa tcggggaat tacaaccaa 
gtgcctcta atattcggg tttacgaca agagaggga 
atgttgatt gagcaaatg ataaagccg agaaagccc 
accatgccg cgggatttt gaggcagcg attccttac 
actctggag gttgaacgg ggttcggaa aattcgaaa 
caaatcgct cagaagatc aaagatttt tattacgga 
gagccaggc tccgaagac aagcttgaa aatttctac 
attattcat accgacaac tatttcctg agagatatt 
atttttgcg gtcaaacac catgccgct acgtccaaa 
ttcccggtt tatttgtac caaatgtcg gtagattca 
agtttgaat gtcatgaaa agactttca aacataact 
gcgccaggt gtagctcac ggtgacgac atcggctac 
ctcttcaaa accaaagtc tcgccagct ttaaacccc 
ggaagtttg gaggaagta tcagtgaga cgtttcgtc 
agactttgg acgaacttc gccagaaac ggcaatcca 
gacgaaccg cagtggaaa ccggtgaaa acgaacgaa 
ctcaattac atcgacatt ggggagaaa ttaagtgcg 
aaagttaac cccgagccc gaaagaatg aaattctgg 
gaagaactt tacaaaaat aaaccactg tattcaatt 
taa
    mRNA sequence
    • >TC033626 RA
      agtgagcca cgatgaagc gtgtgtttt tttcgtacc 
agtttttgg ccactgtga cctcctcgt tggctgttt 
tatcgtcgg ggattcacg aagcctgga cttcggtct 
acatccccg ttttgctga acggcacca acactttga 
aaataacaa gtgtggaag ggtcctgga ttacgatgt 
ccatgagtt ttggcggat tgatcggct gtgttgtgt 
cttgccttc tgattaata aaatcggac gcaaaaaaa 
cgatactcc tgacgtttt gtcccaatt ttttgtcct 
cggtggtcc tggcttttg ccaattcgg tgccggttt 
tttgcacag ctcgggttt tatcgggtg ttgctttcg 
ggattgcaa tcggggtta ttccccatt atatcggcg 
aaatcgcag atccggaaa ttaggggtt cgttgggca 
cacttgtga ctatttttt cgctgtctg gtttcctct 
ttataaaca ttgtcggtt cgtacgtaa cgatacaaa 
catcgtctt ggatttcgg caacaattc cagttctat 
tcctactga cttttattt ggatgccgg aaagtccgt 
actatttgg taatgattg gagagtgcg atcaggctg 
aacaaactt tggcaaaac ttaagggaa cacgcgatg 
tttttgacg agttccaaa atttgaaag atgtcatgc 
cagtttctg ttatcacta ttgccctaa ccttcggtt 
taaacgtct ggcacagtt tcatcatgt ctgatccca 
ccgtcacca tacagcagg gacaactcc ggggaaaaa 
tctcgacca acattgaca ataaaaact tctacagtt 
tccaaggca ttccatacg ccaaacccc ccactggac 
cactccggt tcaaggacc ctcaagccc cggaaccat 
ggaccggag tcaaggacg caacgacgg aaggaagcg 
tgtgttacg ccaaacaca tgcttttca ataacatta 
tcggatcgg aggattgcc tacacttga atgtttaca 
caccggaat tggcatcaa aaaatttaa gaccagtca 
tggtctgga tacatggtg gcgccttta aaagcggct 
caagtaata cggatgtct acggtcctg agtatttgc 
taactcagg atgttgttg tcgttactt tcaactaca 
gattgggaa ttttcgggt ttcttaaac tggatgatg 
ccacgttag gtgttcccg ggaacgcgg gaatgaaag 
acatggtaa tggccttga aatgggtcc ggaaaaaca 
tcagtaaat tcggtgggg atccgaaca atgtgacca 
tttttggtg aaagtgctg gagcagcct cggtgcatt 
acctagtct tgtcaccac tagccaaag ggctgtttc 
acagggcca ttgcacaga gtgggactg cgttaaatt 
cttttgcaa gggggcgca gtgacatgg tttcgcata 
tctcggctg agttgaaca ccaaaaatg agagggaaa 
ttttggaaa agttggtgc aaatgccag ccgaacaac 
tgtttgaac tgtacgaga aattagtaa ctgtgtgtg 
agggtttga acaatgccg gtggtaaac tacccttcg 
ctccggtga ttgaaaagg cgtctcaaa atgcgttca 
ttgccgaag aaccgctca aaatcataa aatcgggga 
attacaacc aagtgcctc taatattcg ggtttacga 
caagagagg gaatgttga ttgagcaaa tgataaagc 
cgagaaagc ccaccatgc cgcgggatt ttgaggcag 
cgattcctt acactctgg aggttgaac ggggttcgg 
aaaattcga aacaaatcg ctcagaaga tcaaagatt 
tttattacg gagagccag gctccgaag acaagcttg 
aaaatttct acattattc ataccgaca actatttcc 
tgagagata ttatttttg cggtcaaac accatgccg 
ctacgtcca aattcccgg tttatttgt accaaatgt 
cggtagatt caagtttga atgtcatga aaagacttt 
caaacataa ctgcgccag gtgtagctc acggtgacg 
acatcggct acctcttca aaaccaaag tctcgccag 
ctttaaacc ccggaagtt tggaggaag tatcagtga 
gacgtttcg tcagacttt ggacgaact tcgccagaa 
acggcaatc cagacgaac cgcagtgga aaccggtga 
aaacgaacg aactcaatt acatcgaca ttggggaga 
aattaagtg cgaaagtta accccgagc ccgaaagaa 
tgaaattct gggaagaac tttacaaaa ataaaccac 
tgtattcaa tttaatgta atttcaaaa attctaata 
aaaataaaa ttgttat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY