| General information | |||
| TC032667
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 29337218..29443319 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032667 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02745 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07095 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)| 2 |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032667 RA
MSAYRNYVP WNKSPNSST SFSSKKEGT KYDSSFGKK KDEEKSATA RIASKYGAY GSGYQRDSS ASGSSSYSS RLKPTLEKK ETNHPPVSY RALARTTPR SRDPSPDKS SSSSSYNYN RLYPTRPYT RSVSRELDT STTIPKYSG RSSLSKDDV SSRRSSVSR EDLSKYTGR SSTTKEDLV PKYSGRSSL TKDDLSRYK PTSREDLSS SSQKYINSR FLPKNSVEK SYTAYTRPS AIRSHETIR KNRELLNVL HAQQEQSRP TSRCDAKTP PKEPETETV STITRATSP SPTTTNIAR SRRTEIAKT VEKQTTRAK NRPMSDKEV QSDDTKNPR VSGAGRMVA TPWSSFLDM KFSSPNDQT RKSVSRTNS SKSLEKKSP PKKTSPTKQ LLPPQIPKS DSKSSSLHS ISTANKDFR KSVLNMNPE GGKKKVGRR SNSASSAES EPEVQASEA TDVSENLTS CPSYHKSSS SSSKLPQRV DKGGQRSRR SPSSDGSTS SSSASEEEK KDLKVNSSR TSMAVSSAD ESPKPPPSP RLKSEAEAK SFLMRALAP VTSLFKTKY QGSSDKINW MDESPPTTP KSDQKPAKN FKKIESGER AWWLDDTTA SCSLPKDSE KSQPKTKPF LRNIDSGEI PWWLDKNAE VPEGVQTYP EESPQKIFR LGSTDTEPS SNIMDADYQ ERHKIRHID SGERSWWLS SAENIPEMV QAQEKSEKP KYAIRHQES GEKAWWLQS NSHSEEFDQ TQAPLGDRA SPEGLEMPE GRLSPYDNV PVRSNKGKK TANLFISKH RNIDEILGG CGPMWIFDY QGQEKEEKC MEVDAKEVR IHDSTPQRG VIQPARIDN IGGFPKTFS PYGKYLNDI DPFSYTTNF PDPARPPLH TFSVTLPLI NQLAAVHRL LKSPHRLDD AALQLYKDG DYGSYLDLE ASISEQQEE FEGFQSNES SLNPRLTKP LNMGYLLHE LIAYNNRVD AAPVPVLCL ISDVRIFCE KSEEGLLTQ WLNSLETRS SLFWLHYVD HDDDDKRAN SLESTEAST SEDDHVDDF LDVDDVPTC RSIAHRESD LLLALTENC TLGPVCYFC RLIPIFCCS KKNSIVLRT QLSVRVHTI IEKLLTSEG RELRRALFS LKQIFQEDK DLVHEFVQN DGLSCLIKV GSEADQNYQ NYILRALGQ VMLYVDGMN GVMEHNQTI QWLYSLISS KFRLVVKTA LKLLLVFVE YTESNCLLL IKAIQAVDF AQGVLPWHN IMKLLKDFD SADTELLIY ATTLINKVL NGVPDQDTY YDQTDSLEE QEIERIIQR YMSKPGTDL DLLQQFQIY EAVLQYEDG EEGGTPIRH LDESIRKTL RTRKSVSEA NERRKSRRH STGTSPHPH PLLARNNNR SLQINVEDD ESSSSQSSE INNSQLNGC YKDSNKATD AGVTPALRR RRERAERQR SFMREQQET VELLRAASQ NEEDENENN LGNYTNALQ QRIANRKDI TPLMNAVNK LDSEENLTK QPWILYDDS QQPIDENTE NSNENERNV LLQLKRENT VKDLTQKLA NQNILHSPS DENKPNRIG DMTGLISKA KEGLAKSKS KADVLKSPT TESPPKIEV KKSENELRW EELVANLDR PLNLCDMDF TDLGSDDEA DVLAPAAVF NGIPPPPPP LKENGAPPP PPLSKGPPP VPNPPVFGV TLPRMSKQA ETKIPIKKN KKTVKLFWK EVREDPVCS SKLKTGFIW DELKPVCVD TQKLEHLFE SRAKDLITK KQQEMNKNK EIIVLDPKR SNAINIGMT KLPPPRSIK TAILKMDAT IMNREGIEK LLTMLPTEE ERSKIQEAQ AANPDLPLG SAEQFLLTL ASISELPAR LKLWAFKLD FENSEREIA EPLMDLKQG IEVLRVNKT FRGILSTLL SIGNFLNGN EVKGFQIDY LAKVPEVKD TVHKHSLLH HLCHIVMEK FPDATDLYS EIGAITRAS KIDFDELAL NIQRLEADC KASWDHLKL IAKHDGSTM MKVKMSDFL ADCAERIIL VGIIHRRVM NRFHKFLLW LGIPLHQLS DTKPNEFCR IVSEFALEY RTTRERVLQ QLEKKANHR ERNKTRGKM ITDAHAITV ASPLVTF
- >TC032667 RA
atgagcgcc tacagaaac tacgtgcct tggaacaaa tcgccgaat tcctccaca agtttttcg tccaaaaaa gaagggaca aaatacgat tcgagtttt ggcaagaag aaggatgaa gaaaagagt gccacggcc aggattgcc tcgaaatat ggcgcctat ggctcaggc taccagcga gacagcagt gcttcagga agcagcagc tactcgagc cggcttaaa ccaactctg gaaaaaaag gagacgaac caccctccc gtctcttat cgggcttta gcccgaaca accccccgg tcacgtgac cctagccct gacaaaagc tcctcttct tcgagctac aactacaat cgcctttac cccaccagg ccctacacc cgctctgtg agtagggaa ctggacact tccaccacc ataccgaaa tattccgga cgctcctca ctcagtaaa gatgacgtg tccagtcgg cgttcgtcc gtaagccgg gaagactta tccaaatac actggacgt tcttccaca acgaaggaa gacctagtc ccgaaatat tcaggacgg tcttcactc accaaagac gatttgtcg cgctataag ccgacaagt cgcgaagat ttatcttca agttcccag aaatatatc aacagccgg tttcttccg aaaaattct gttgagaaa agctatacg gcctacacc aggccttct gcgatcaga tcgcacgaa accattcgc aaaaatcgc gaactactc aatgtcttg cacgcgcaa caagagcaa agtcggccc acaagtcgc tgcgacgcc aaaacccct cccaaagaa ccggaaacg gaaactgtc tcaaccata accagagcc acttcccct tcccccacc accacaaac atcgctcgc tcccggcga accgaaatc gccaaaacc gtcgaaaaa caaacaaca cgtgccaaa aaccggcca atgagcgac aaggaagtc caatcggac gacactaaa aatcccagg gtttcagga gcgggccgt atggtggcc actccttgg agttcgttc ctcgatatg aaattttcg agtccaaac gaccaaacc cgcaaaagt gtctcacgg acgaactcc agcaaaagt ctggagaag aaatcaccc cccaagaag acttccccc accaagcaa ctcctccca ccccaaatc cccaaaagc gactcgaaa tcatcgtcg ttacattcc attagcacc gcgaataag gatttcagg aaatcggtc ctgaatatg aaccctgaa ggggggaag aagaaggtt gggaggagg agtaattcg gcgagttcg gctgagtcc gagcctgaa gtccaggcg tcggaagca accgacgtt tcggagaat ttgacgagt tgtccgtct tatcacaag agtagttct tcgagttct aagttgccg cagagggtt gataagggg gggcagagg agtcgaagg tcgccgagt tccgatggg agcacctcg tcgtcgtct gcgagcgag gaggagaag aaggatttg aaggttaac tcctcgagg acttccatg gcggtgtcg tcagctgat gagtcgccg aaaccgcca ccaagtcca agactcaag tcggaagcc gaagccaag tcgtttcta atgagggct ttggcgccg gttacgagt ttgttcaag accaagtat caaggatcc agtgacaaa atcaactgg atggacgaa agccccccc accactcca aaaagcgac caaaaaccg gcgaaaaac ttcaaaaaa atcgaatcc ggtgaacgc gcctggtgg ttggacgac accacagcc tcctgttcc ctcccaaag gactctgaa aagagccaa ccgaaaacc aaacccttc ttacgcaat atcgattcc ggggaaatc ccctggtgg ctcgacaaa aacgccgaa gtccctgaa ggcgtccaa acgtacccc gaggaatcc cctcagaaa attttccgt cttgggagt actgacact gagcccagt agcaatatc atggacgct gactaccaa gagcgccac aaaatcaga cacatcgac tcaggagag cgctcttgg tggctcagc agcgctgag aatatcccc gaaatggtg caagctcag gaaaagagc gagaaaccg aaatatgca atcaggcat caggaatca ggggagaaa gcctggtgg ttgcagtcg aactcacac tcagaggag ttcgaccag actcaggcc cctttgggc gatagggcg agtcctgag ggcttggaa atgccagaa gggaggtta agtccctat gataatgtc cctgtgagg agtaataag ggaaagaaa acagcgaat ttgtttatt tccaaacat agaaatatt gacgagatt ttgggaggg tgtgggccg atgtggatt tttgattac caaggtcag gaaaaagag gagaagtgc atggaagtg gacgccaag gaggtcagg attcacgac agtacgccc cagagggga gtcattcag cccgctcgg attgacaac atcggtggt ttcccgaag actttttcg ccgtacgga aagtatttg aacgacatc gaccctttt tcgtacact acgaatttt ccggatcct gcgagacct cctctgcac acttttagt gtcactttg ccgctcatc aaccagctg gcagctgtt cacaggctg ctcaagtcc ccacatagg ttggatgat gcggcactt cagttgtac aaagatggg gattatggg tcttacctt gacctcgaa gcttctatc agtgaacag caagaagag tttgagggt tttcaatca aatgagagt tctcttaac cccaggtta acaaaaccc ctcaatatg ggttatctc ctccacgaa ctcatcgca tacaacaat cgagtagac gctgcacct gtgccagtg ctgtgcttg ataagtgat gtgaggata ttttgcgaa aaatccgag gagggcctc ctcacccaa tggctcaac tctctggag acgcgttcg tcgctcttc tggctccac tatgtggac cacgacgac gacgacaaa cgcgccaat tcgctggaa tcgacggag gcgtcgacc agcgaagac gaccacgtg gacgacttc ctggacgtg gacgacgtg cccacatgt cgcagtatc gcgcaccgg gagtccgac cttttgctg gctctcacc gagaattgc actctgggc ccggtttgc tatttttgc agactaatt cccattttc tgttgcagt aagaagaat tcgatcgtt ttaaggaca cagctgtcg gttagagtg cacacgatt atagaaaaa ttactaact tccgaaggc cgcgaactg cgtcgcgca cttttctcc ctcaaacaa atcttccaa gaggataaa gacctggtc catgagttt gtccaaaac gatgggttg tcgtgtctc atcaaagtc ggctctgaa gccgaccaa aactaccaa aattacatt ttgcgggcc ttgggccaa gtgatgttg tatgtggat gggatgaac ggggtgatg gaacacaac caaaccatc caatggttg tacagtctc atttcgtca aagttccga cttgtggtc aaaaccgcc ttgaagttg cttttggtt tttgtggaa tatacggaa tcgaattgt ttgctgttg atcaaagcg attcaagcg gttgatttt gcacaaggt gttctgcct tggcacaac ataatgaaa ctactcaaa gattttgat tccgctgac accgaactc ctgatctat gctacaact ttgatcaat aaagtacta aatggagta ccagatcaa gacacttat tacgaccaa accgattca cttgaggag caagaaatc gagcgaata atccaaaga tacatgtcc aagccggga actgatctg gacttactc caacaattc caaatttac gaagccgtt cttcaatac gaagacggc gaagaaggc ggaacccca atccgccac ttggacgaa agtatccga aagacgctc cgaacgcga aaatcagtc tcggaagcg aacgaacgc agaaaaagt cggagacac tccaccggg acttccccg caccctcac ccccttcta gcgcgcaac aacaacagg tccctccag atcaacgtc gaagacgac gaatcgtca agctcccag tcgtcggaa atcaacaat tcgcagctg aatggatgc tataaggac agtaacaaa gcgactgac gctggcgtc acccccgct ttgaggagg aggcgggaa cgggcggag agacagagg agtttcatg cgggagcag caggagacg gtggagttg ttgagggcg gcgtcccag aatgaggag gacgagaat gagaataat ttgggaaac tacacaaac gccctccag cagcgaatc gccaaccgc aaagacatc acccctctc atgaacgca gtcaacaag cttgactct gaagagaac ctcactaaa caaccctgg atcctttac gacgactcc caacagcca atcgacgaa aacaccgaa aattcgaac gaaaacgag cgaaatgtc ctcctccaa ctcaaacgc gaaaacaca gtcaaagac ctgacccaa aaattggcc aatcaaaat atcctccat agtccttca gacgaaaac aaaccgaac cgaatcggc gacatgacc ggtttaatt tccaaggcc aaagaaggc ttagccaag tccaagtcc aaagcggac gtgctcaag agcccaaca actgaatcc ccgccaaaa atcgaagta aagaagtcc gaaaacgag ctcagatgg gaggagtta gtcgcgaat ttagatcgt ccgttgaac ttgtgtgat atggacttc actgatttg gggagcgac gacgaggcg gatgttctg gccccagct gctgtgttc aatggaatc cctccacct cctccccct ttgaaggag aacggggcg cccccacct cccccctta tcaaagggg ccccctccc gtcccaaac cccccagtt tttggggta accctccca cgaatgtcc aaacaagcc gaaaccaaa atcccaatc aagaaaaac aagaagaca gtaaaactc ttctggaag gaagtccga gaggatcca gtttgctcc tccaaactc aagactggt tttatttgg gacgaactg aaaccagtt tgtgtcgac acccaaaaa ctggaacac ctgttcgag tcaagggcc aaggacctc attacgaag aaacagcaa gagatgaat aaaaacaag gaaataatc gtcctggac ccgaaacgc tccaacgcc atcaacatc ggcatgact aagttaccc cccccacgt tccatcaaa accgccatt ttaaaaatg gacgccacc ataatgaac cgggaaggt atcgaaaaa ttgctcaca atgctgcca actgaagag gaaaggtct aaaatacaa gaagcccaa gctgccaat cctgatctt cctctaggg agtgcggaa cagttcctt ttaactcta gcatcaatc tctgaatta ccggcaaga cttaaattg tgggctttt aagctcgat tttgaaaat tccgaacgg gaaattgcc gaacccttg atggatttg aagcaaggg attgaggtt ttgcgagtg aataagacg tttaggggg attttgagc acccttttg tcgataggg aattttttg aatgggaat gaggtgaaa gggttccaa attgattat cttgccaaa gtacctgaa gtcaaagat acggtccat aagcactct ttgttgcac catctttgc catattgtt atggagaag ttcccagac gcgactgat ttgtattca gagattggt gcaattact cgagcttcc aaaatcgac ttcgacgaa cttgccttg aacatccag aggcttgag gccgattgc aaggcctcc tgggaccac ttgaaactc atagccaaa cacgatggt tccactatg atgaaagtc aaaatgtcc gactttcta gccgattgt gccgaaaga attattctg gttggaatc atccacagg agggttatg aacagattt cataaattt ttattatgg ttagggatc cctctacat caactgtcg gataccaag ccaaatgag ttttgtaga atcgtttcc gagtttgcc ttagaatat cgaacgaca agggaacga gttttacaa cagttggaa aagaaagcc aatcatcgc gaacgcaat aaaacgagg gggaaaatg ataacggat gcccacgcc atcacagta gcttctccc ctagttact ttctga
- >TC032667 RA
gtggttaga atagagcga gaacgtttt aataaattc tatttgggc cgcgccagt tttttcaat tagtttaac catttcggt ttagtgtgt gttgttgga tgatctttg gtttatttg tgggttcaa acggtttcg acctcaaca caatgtaaa cttggtcga ttttttgac aaattgtga ctaattaat taatatgaa gtgaggtgt gagctattt tcaaattat gagcgccta cagaaacta cgtgccttg gaacaaatc gccgaattc ctccacaag tttttcgtc caaaaaaga agggacaaa atacgattc gagttttgg caagaagaa ggatgaaga aaagagtgc cacggccag gattgcctc gaaatatgg cgcctatgg ctcaggcta ccagcgaga cagcagtgc ttcaggaag cagcagcta ctcgagccg gcttaaacc aactctgga aaaaaagga gacgaacca ccctcccgt ctcttatcg ggctttagc ccgaacaac cccccggtc acgtgaccc tagccctga caaaagctc ctcttcttc gagctacaa ctacaatcg cctttaccc caccaggcc ctacacccg ctctgtgag tagggaact ggacacttc caccaccat accgaaata ttccggacg ctcctcact cagtaaaga tgacgtgtc cagtcggcg ttcgtccgt aagccggga agacttatc caaatacac tggacgttc ttccacaac gaaggaaga cctagtccc gaaatattc aggacggtc ttcactcac caaagacga tttgtcgcg ctataagcc gacaagtcg cgaagattt atcttcaag ttcccagaa atatatcaa cagccggtt tcttccgaa aaattctgt tgagaaaag ctatacggc ctacaccag gccttctgc gatcagatc gcacgaaac cattcgcaa aaatcgcga actactcaa tgtcttgca cgcgcaaca agagcaaag tcggcccac aagtcgctg cgacgccaa aacccctcc caaagaacc ggaaacgga aactgtctc aaccataac cagagccac ttccccttc ccccaccac cacaaacat cgctcgctc ccggcgaac cgaaatcgc caaaaccgt cgaaaaaca aacaacacg tgccaaaaa ccggccaat gagcgacaa ggaagtcca atcggacga cactaaaaa tcccagggt ttcaggagc gggccgtat ggtggccac tccttggag ttcgttcct cgatatgaa attttcgag tccaaacga ccaaacccg caaaagtgt ctcacggac gaactccag caaaagtct ggagaagaa atcaccccc caagaagac ttcccccac caagcaact cctcccacc ccaaatccc caaaagcga ctcgaaatc atcgtcgtt acattccat tagcaccgc gaataagga tttcaggaa atcggtcct gaatatgaa ccctgaagg ggggaagaa gaaggttgg gaggaggag taattcggc gagttcggc tgagtccga gcctgaagt ccaggcgtc ggaagcaac cgacgtttc ggagaattt gacgagttg tccgtctta tcacaagag tagttcttc gagttctaa gttgccgca gagggttga taagggggg gcagaggag tcgaaggtc gccgagttc cgatgggag cacctcgtc gtcgtctgc gagcgagga ggagaagaa ggatttgaa ggttaactc ctcgaggac ttccatggc ggtgtcgtc agctgatga gtcgccgaa accgccacc aagtccaag actcaagtc ggaagccga agccaagtc gtttctaat gagggcttt ggcgccggt tacgagttt gttcaagac caagtatca aggatccag tgacaaaat caactggat ggacgaaag cccccccac cactccaaa aagcgacca aaaaccggc gaaaaactt caaaaaaat cgaatccgg tgaacgcgc ctggtggtt ggacgacac cacagcctc ctgttccct cccaaagga ctctgaaaa gagccaacc gaaaaccaa acccttctt acgcaatat cgattccgg ggaaatccc ctggtggct cgacaaaaa cgccgaagt ccctgaagg cgtccaaac gtaccccga ggaatcccc tcagaaaat tttccgtct tgggagtac tgacactga gcccagtag caatatcat ggacgctga ctaccaaga gcgccacaa aatcagaca catcgactc aggagagcg ctcttggtg gctcagcag cgctgagaa tatccccga aatggtgca agctcagga aaagagcga gaaaccgaa atatgcaat caggcatca ggaatcagg ggagaaagc ctggtggtt gcagtcgaa ctcacactc agaggagtt cgaccagac tcaggcccc tttgggcga tagggcgag tcctgaggg cttggaaat gccagaagg gaggttaag tccctatga taatgtccc tgtgaggag taataaggg aaagaaaac agcgaattt gtttatttc caaacatag aaatattga cgagatttt gggagggtg tgggccgat gtggatttt tgattacca aggtcagga aaaagagga gaagtgcat ggaagtgga cgccaagga ggtcaggat tcacgacag tacgcccca gaggggagt cattcagcc cgctcggat tgacaacat cggtggttt cccgaagac tttttcgcc gtacggaaa gtatttgaa cgacatcga ccctttttc gtacactac gaattttcc ggatcctgc gagacctcc tctgcacac ttttagtgt cactttgcc gctcatcaa ccagctggc agctgttca caggctgct caagtcccc acataggtt ggatgatgc ggcacttca gttgtacaa agatgggga ttatgggtc ttaccttga cctcgaagc ttctatcag tgaacagca agaagagtt tgagggttt tcaatcaaa tgagagttc tcttaaccc caggttaac aaaacccct caatatggg ttatctcct ccacgaact catcgcata caacaatcg agtagacgc tgcacctgt gccagtgct gtgcttgat aagtgatgt gaggatatt ttgcgaaaa atccgagga gggcctcct cacccaatg gctcaactc tctggagac gcgttcgtc gctcttctg gctccacta tgtggacca cgacgacga cgacaaacg cgccaattc gctggaatc gacggaggc gtcgaccag cgaagacga ccacgtgga cgacttcct ggacgtgga cgacgtgcc cacatgtcg cagtatcgc gcaccggga gtccgacct tttgctggc tctcaccga gaattgcac tctgggccc ggtttgcta tttttgcag actaattcc cattttctg ttgcagtaa gaagaattc gatcgtttt aaggacaca gctgtcggt tagagtgca cacgattat agaaaaatt actaacttc cgaaggccg cgaactgcg tcgcgcact tttctccct caaacaaat cttccaaga ggataaaga cctggtcca tgagtttgt ccaaaacga tgggttgtc gtgtctcat caaagtcgg ctctgaagc cgaccaaaa ctaccaaaa ttacatttt gcgggcctt gggccaagt gatgttgta tgtggatgg gatgaacgg ggtgatgga acacaacca aaccatcca atggttgta cagtctcat ttcgtcaaa gttccgact tgtggtcaa aaccgcctt gaagttgct tttggtttt tgtggaata tacggaatc gaattgttt gctgttgat caaagcgat tcaagcggt tgattttgc acaaggtgt tctgccttg gcacaacat aatgaaact actcaaaga ttttgattc cgctgacac cgaactcct gatctatgc tacaacttt gatcaataa agtactaaa tggagtacc agatcaaga cacttatta cgaccaaac cgattcact tgaggagca agaaatcga gcgaataat ccaaagata catgtccaa gccgggaac tgatctgga cttactcca acaattcca aatttacga agccgttct tcaatacga agacggcga agaaggcgg aaccccaat ccgccactt ggacgaaag tatccgaaa gacgctccg aacgcgaaa atcagtctc ggaagcgaa cgaacgcag aaaaagtcg gagacactc caccgggac ttccccgca ccctcaccc ccttctagc gcgcaacaa caacaggtc cctccagat caacgtcga agacgacga atcgtcaag ctcccagtc gtcggaaat caacaattc gcagctgaa tggatgcta taaggacag taacaaagc gactgacgc tggcgtcac ccccgcttt gaggaggag gcgggaacg ggcggagag acagaggag tttcatgcg ggagcagca ggagacggt ggagttgtt gagggcggc gtcccagaa tgaggagga cgagaatga gaataattt gggaaacta cacaaacgc cctccagca gcgaatcgc caaccgcaa agacatcac ccctctcat gaacgcagt caacaagct tgactctga agagaacct cactaaaca accctggat cctttacga cgactccca acagccaat cgacgaaaa caccgaaaa ttcgaacga aaacgagcg aaatgtcct cctccaact caaacgcga aaacacagt caaagacct gacccaaaa attggccaa tcaaaatat cctccatag tccttcaga cgaaaacaa accgaaccg aatcggcga catgaccgg tttaatttc caaggccaa agaaggctt agccaagtc caagtccaa agcggacgt gctcaagag cccaacaac tgaatcccc gccaaaaat cgaagtaaa gaagtccga aaacgagct cagatggga ggagttagt cgcgaattt agatcgtcc gttgaactt gtgtgatat ggacttcac tgatttggg gagcgacga cgaggcgga tgttctggc cccagctgc tgtgttcaa tggaatccc tccacctcc tcccccttt gaaggagaa cggggcgcc cccacctcc ccccttatc aaaggggcc ccctcccgt cccaaaccc cccagtttt tggggtaac cctcccacg aatgtccaa acaagccga aaccaaaat cccaatcaa gaaaaacaa gaagacagt aaaactctt ctggaagga agtccgaga ggatccagt ttgctcctc caaactcaa gactggttt tatttggga cgaactgaa accagtttg tgtcgacac ccaaaaact ggaacacct gttcgagtc aagggccaa ggacctcat tacgaagaa acagcaaga gatgaataa aaacaagga aataatcgt cctggaccc gaaacgctc caacgccat caacatcgg catgactaa gttaccccc cccacgttc catcaaaac cgccatttt aaaaatgga cgccaccat aatgaaccg ggaaggtat cgaaaaatt gctcacaat gctgccaac tgaagagga aaggtctaa aatacaaga agcccaagc tgccaatcc tgatcttcc tctagggag tgcggaaca gttcctttt aactctagc atcaatctc tgaattacc ggcaagact taaattgtg ggcttttaa gctcgattt tgaaaattc cgaacggga aattgccga acccttgat ggatttgaa gcaagggat tgaggtttt gcgagtgaa taagacgtt tagggggat tttgagcac ccttttgtc gatagggaa ttttttgaa tgggaatga ggtgaaagg gttccaaat tgattatct tgccaaagt acctgaagt caaagatac ggtccataa gcactcttt gttgcacca tctttgcca tattgttat ggagaagtt cccagacgc gactgattt gtattcaga gattggtgc aattactcg agcttccaa aatcgactt cgacgaact tgccttgaa catccagag gcttgaggc cgattgcaa ggcctcctg ggaccactt gaaactcat agccaaaca cgatggttc cactatgat gaaagtcaa aatgtccga ctttctagc cgattgtgc cgaaagaat tattctggt tggaatcat ccacaggag ggttatgaa cagatttca taaattttt attatggtt agggatccc tctacatca actgtcgga taccaagcc aaatgagtt ttgtagaat cgtttccga gtttgcctt agaatatcg aacgacaag ggaacgagt tttacaaca gttggaaaa gaaagccaa tcatcgcga acgcaataa aacgagggg gaaaatgat aacggatgc ccacgccat cacagtagc ttctcccct agttacttt ctgactacc aacctcata acctaaact aactaaata ttctaacac tcggtcatt ctgaccttc gagcggacc tccgctcga cataaaaca aggagcaat gaaatttaa tacactaat gaaacaaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
