Status
Please wait ...
Gene information
General information
TC032332
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003353,TC003352
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1970657..2018839
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032332
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032332 RA
      MPGFNLIFF LLVLVHVVI GKEDPYFSE SPRNVDVVQ 
      GKSVTLPCK VTPGIGMTY YWELNGSKI ANTTRRHQQ 
      GSNLHITRV DRERDSGQF TCIAEDSTS SLAGSIRSS 
      EASLNIQWI DEVSVQLQE PESASFISR GSDVTLRCH 
      TDASGDVHY EWFRNSDRL LKSDKIEIK KKRLHIKNV 
      GPSDNGVYR CLANNTAGA RYSTTNFAL AVAGEETAL 
      IQVVPRDQL VPQGGTAYF DCQYQQAEV TEWYFKKDT 
      RPLENGKKF VIHSNGTLQ INDVQGKDQ GFYSCVGIR 
      SESTEIPQS YIAELKLAY LSEFTVNSF EPPLSENSN 
      QVVAPEGGL FQLTCLEPD SLPPAKKWW LNTGGHTVS 
      DSGEVRVDD GRLIIEKVR MDHAGNYTC VAENMAGKT 
      EKIIELVVL NKPVVTAQP LSVTVDENE PSMLTCDYE 
      PKNHFTIVK WRKDGKSLR HDFGENSLD RQRIKIFKH 
      NASLVIRET ESHDRGEYV CEVLAKGFE PVLSEPATI 
      SVIEKLKFV PPPVNKKLE LGSVAKVHC KAQGTPPPI 
      ITWEKLGES FDNSRIISM NGTLVFNGV LNEDKGKYT 
      CVATNSQGV INVTINIDV VVAPKFSVE PKNPTEVTE 
      GQSVTIDCV VEGDPKPTI HWDKNLKMN DFDHSRFTV 
      LENGTLFIS EVHREDENK YGCTAGNSA GLNRQEVQL 
      IVHSRDGYH PDGDSTVTK AVLITMSVA GAYIVLVIG 
      LMVWCRYRR RSRKLPVTD AAKTENGEV DHTELKDGA 
      NGHCSGTSK VATNGLEAL KEGQKSDGA ETTHSQSSN 
      QSKKSKSNY DKLALSRTH LKEMKLIGR GEFGEVMVA 
      KMSKSALLS EKRNSQATT PPIEDKELP VLVKALSHT 
      KDENSLSEF KREIDMFLK LSHENITKI YGLCREAEP 
      HYMILEHTD WGDLKQFLV ATQKGSPPP LTPVQCVAI 
      VHQLCQGLD HLATSRLIH RDLAARNCL VTSTLKAKV 
      GLPRLTRDP YSQEYCKHV NHIIPLRWL PYEAVYEDE 
      YSTKSDVYS FAVVIWEIF NQGELPFPK MNDTSFLNK 
      LKEKKLEWK PHSSTPESL QKLQASCWD SNPQNRPTF 
      SELAKEIEE VLKSM
    CDS sequence
    • >TC032332 RA
      atgcctggc tttaacttg atttttttt cttctcgtg 
      ttggttcac gtggttatc ggcaaggaa gatccatat 
      ttcagtgaa agccccagg aatgtggac gttgtgcaa 
      ggaaaatcc gtgacatta ccttgtaaa gtgacaccg 
      ggaataggg atgacgtac tactgggaa ctaaacggt 
      tctaaaata gccaacaca acgcgtcgg caccagcag 
      ggctccaac cttcatata actcgagtg gaccgcgaa 
      cgcgactcg ggtcagttc acctgcatt gctgaagac 
      tcgacctca tcattagcc ggctccatc cgaagttca 
      gaagcctcg ctcaacatt caatggatt gacgaagtg 
      tccgtacag ctccaggag ccagaatcg gcttcgttc 
      atatcgcgt gggagcgat gtcactctc aggtgtcac 
      acggacgct tccggggac gtccattac gagtggttt 
      agaaattca gaccgactc cttaaatcc gacaaaatt 
      gaaatcaaa aagaaaaga ctgcacatt aaaaatgta 
      ggaccttcg gacaatggc gtctacaga tgtttggcc 
      aataacaca gcaggggcc agatacagc actacaaat 
      tttgcccta gctgttgca ggtgaggaa actgcgcta 
      atccaggtg gtgccaagg gaccagttg gtaccgcaa 
      ggaggtacc gcgtatttt gactgccag taccaacaa 
      gctgaagtg accgaatgg tacttcaag aaggacaca 
      aggccactg gagaatggc aaaaaattt gtgatacac 
      tcaaatggt actctccaa attaacgat gttcaagga 
      aaagaccaa gggttttac agttgtgtt ggaattcga 
      agcgagtca actgaaata cctcaaagc tacatagcc 
      gaattaaaa ctagcctac ttgagcgaa ttcaccgtc 
      aattctttc gaacccccc ctttcggaa aactcaaac 
      caggtcgtg gcccctgaa ggagggctg tttcaactg 
      acttgcctc gaacctgac agtttaccc ccggccaaa 
      aaatggtgg ctcaacacc gggggtcac actgtgtca 
      gattccggg gaagtccgg gttgatgac ggcagatta 
      ataatagaa aaagtgcga atggaccat gcggggaat 
      tacacatgt gtcgccgaa aacatggct gggaaaacc 
      gagaaaatt atcgaactg gtcgttttg aacaaacct 
      gtagttact gcacaaccc ctcagtgtc acagttgac 
      gaaaacgaa ccctcgatg ttaacttgt gactacgag 
      cccaaaaac cacttcacg attgttaag tggcggaag 
      gatgggaag agtttacga cacgatttt ggggaaaat 
      tcgctcgat cggcaacga attaaaatt tttaaacac 
      aacgcttct ttagtgatt cgagagact gaaagtcac 
      gacaggggc gagtatgtg tgtgaggtg ttggctaag 
      ggttttgaa cctgtctta tccgaacct gccactatt 
      tctgtgatc gaaaaattg aagtttgtg ccgccgcca 
      gttaacaaa aaactagag cttggcagt gtggcgaaa 
      gttcactgt aaggcgcaa ggaacacca cctccgatt 
      atcacgtgg gagaaactc ggggaaagt ttcgacaat 
      tcgcggatt attagcatg aacgggacg cttgtcttt 
      aacggagtt ttgaacgaa gataaaggc aagtacacc 
      tgtgttgcc accaacagt caaggagtt atcaacgtg 
      acgataaac atcgatgtt gttgtggca cctaaattt 
      agcgtggaa ccgaaaaac ccaaccgaa gtcaccgaa 
      ggacagtcg gtgacgatt gactgtgtc gtggaaggc 
      gaccccaaa ccgaccatt cattgggac aagaattta 
      aaaatgaat gattttgat cactcgagg tttactgtt 
      ttggagaat gggactttg ttcataagt gaagtgcac 
      cgagaggat gaaaataag tacggttgc actgctgga 
      aatagcgct ggtttgaat cgacaagaa gtacaactg 
      attgttcat tcacgtgat ggctaccac cctgatgga 
      gattccacc gtaaccaaa gcggtgcta attacgatg 
      tcggtagct ggggcttat attgttcta gttattggg 
      ctcatggta tggtgcagg taccgaaga aggtccaga 
      aaattacca gttactgat gctgcgaaa actgaaaat 
      ggtgaagtg gaccataca gaactgaaa gacggagcc 
      aacggccac tgttcaggc acctccaaa gtcgcaacc 
      aacggactc gaagcttta aaagaaggc caaaagagt 
      gacggtgcc gaaaccact cattcccaa agctcaaat 
      caatcgaaa aaatccaaa tcaaactac gacaaactc 
      gccttatcg cgaacgcac ttaaaagaa atgaaacta 
      atcggccga ggagaattc ggcgaagtc atggtggcc 
      aaaatgtcc aaaagtgcc ctcttgtcc gaaaaacgc 
      aactctcaa gcaaccacc ccaccaatc gaagacaag 
      gaactgcca gtcctcgtg aaagctctg agccacacc 
      aaggacgaa aactcgcta tcagagttc aagcgcgaa 
      attgacatg tttttgaaa ttatcccac gaaaacatc 
      acaaagatt tacggactg tgtcgtgag gccgagccc 
      cactacatg atcctcgaa cacaccgat tggggcgat 
      ttgaagcag tttttggtg gcgacccag aaaggctcg 
      ccgccgcca ctgacccca gtgcagtgc gtggcaata 
      gtacaccag ctgtgtcaa ggtttggac catttggcc 
      acttctcgc ctgatccat agggattta gcggcgaga 
      aattgtcta gtcacttcc acgttaaaa gccaaagtt 
      ggtcttccg cgacttacg agggatccc tacagtcaa 
      gaatactgc aaacatgtg aaccacata attccgctg 
      cggtggttg ccctacgag gctgtttat gaggacgag 
      tactcgaca aagagcgac gtgtactcg tttgccgtc 
      gtcatttgg gagattttc aaccagggc gagctgccg 
      ttccccaaa atgaacgac acgagtttc ctaaataag 
      ctcaaagag aagaaactc gagtggaaa ccgcactct 
      tccacgcct gaatcttta caaaagtta caggcgtcc 
      tgttgggac agtaatccg caaaacagg ccgacgttc 
      tccgaatta gcgaaagaa atcgaagag gtacttaag 
      tctatgtaa
    mRNA sequence
    • >TC032332 RA
      ggtctggct catcaagct ttagccagt ttcacatgt 
      ttgctggta gagtgtaga gctttcggg agcttacgt 
      tacgttatt aatcgtttt actacggtt tctcggttt 
      tattacata caaacttta cacgaaatg tgaactaaa 
      ctcaaactt attcgtatt tatttgtga actcgtgat 
      agtgattgt ttatttacc ggtctcata atgcctggc 
      tttaacttg atttttttt cttctcgtg ttggttcac 
      gtggttatc ggcaaggaa gatccatat ttcagtgaa 
      agccccagg aatgtggac gttgtgcaa ggaaaatcc 
      gtgacatta ccttgtaaa gtgacaccg ggaataggg 
      atgacgtac tactgggaa ctaaacggt tctaaaata 
      gccaacaca acgcgtcgg caccagcag ggctccaac 
      cttcatata actcgagtg gaccgcgaa cgcgactcg 
      ggtcagttc acctgcatt gctgaagac tcgacctca 
      tcattagcc ggctccatc cgaagttca gaagcctcg 
      ctcaacatt caatggatt gacgaagtg tccgtacag 
      ctccaggag ccagaatcg gcttcgttc atatcgcgt 
      gggagcgat gtcactctc aggtgtcac acggacgct 
      tccggggac gtccattac gagtggttt agaaattca 
      gaccgactc cttaaatcc gacaaaatt gaaatcaaa 
      aagaaaaga ctgcacatt aaaaatgta ggaccttcg 
      gacaatggc gtctacaga tgtttggcc aataacaca 
      gcaggggcc agatacagc actacaaat tttgcccta 
      gctgttgca ggtgaggaa actgcgcta atccaggtg 
      gtgccaagg gaccagttg gtaccgcaa ggaggtacc 
      gcgtatttt gactgccag taccaacaa gctgaagtg 
      accgaatgg tacttcaag aaggacaca aggccactg 
      gagaatggc aaaaaattt gtgatacac tcaaatggt 
      actctccaa attaacgat gttcaagga aaagaccaa 
      gggttttac agttgtgtt ggaattcga agcgagtca 
      actgaaata cctcaaagc tacatagcc gaattaaaa 
      ctagcctac ttgagcgaa ttcaccgtc aattctttc 
      gaacccccc ctttcggaa aactcaaac caggtcgtg 
      gcccctgaa ggagggctg tttcaactg acttgcctc 
      gaacctgac agtttaccc ccggccaaa aaatggtgg 
      ctcaacacc gggggtcac actgtgtca gattccggg 
      gaagtccgg gttgatgac ggcagatta ataatagaa 
      aaagtgcga atggaccat gcggggaat tacacatgt 
      gtcgccgaa aacatggct gggaaaacc gagaaaatt 
      atcgaactg gtcgttttg aacaaacct gtagttact 
      gcacaaccc ctcagtgtc acagttgac gaaaacgaa 
      ccctcgatg ttaacttgt gactacgag cccaaaaac 
      cacttcacg attgttaag tggcggaag gatgggaag 
      agtttacga cacgatttt ggggaaaat tcgctcgat 
      cggcaacga attaaaatt tttaaacac aacgcttct 
      ttagtgatt cgagagact gaaagtcac gacaggggc 
      gagtatgtg tgtgaggtg ttggctaag ggttttgaa 
      cctgtctta tccgaacct gccactatt tctgtgatc 
      gaaaaattg aagtttgtg ccgccgcca gttaacaaa 
      aaactagag cttggcagt gtggcgaaa gttcactgt 
      aaggcgcaa ggaacacca cctccgatt atcacgtgg 
      gagaaactc ggggaaagt ttcgacaat tcgcggatt 
      attagcatg aacgggacg cttgtcttt aacggagtt 
      ttgaacgaa gataaaggc aagtacacc tgtgttgcc 
      accaacagt caaggagtt atcaacgtg acgataaac 
      atcgatgtt gttgtggca cctaaattt agcgtggaa 
      ccgaaaaac ccaaccgaa gtcaccgaa ggacagtcg 
      gtgacgatt gactgtgtc gtggaaggc gaccccaaa 
      ccgaccatt cattgggac aagaattta aaaatgaat 
      gattttgat cactcgagg tttactgtt ttggagaat 
      gggactttg ttcataagt gaagtgcac cgagaggat 
      gaaaataag tacggttgc actgctgga aatagcgct 
      ggtttgaat cgacaagaa gtacaactg attgttcat 
      tcacgtgat ggctaccac cctgatgga gattccacc 
      gtaaccaaa gcggtgcta attacgatg tcggtagct 
      ggggcttat attgttcta gttattggg ctcatggta 
      tggtgcagg taccgaaga aggtccaga aaattacca 
      gttactgat gctgcgaaa actgaaaat ggtgaagtg 
      gaccataca gaactgaaa gacggagcc aacggccac 
      tgttcaggc acctccaaa gtcgcaacc aacggactc 
      gaagcttta aaagaaggc caaaagagt gacggtgcc 
      gaaaccact cattcccaa agctcaaat caatcgaaa 
      aaatccaaa tcaaactac gacaaactc gccttatcg 
      cgaacgcac ttaaaagaa atgaaacta atcggccga 
      ggagaattc ggcgaagtc atggtggcc aaaatgtcc 
      aaaagtgcc ctcttgtcc gaaaaacgc aactctcaa 
      gcaaccacc ccaccaatc gaagacaag gaactgcca 
      gtcctcgtg aaagctctg agccacacc aaggacgaa 
      aactcgcta tcagagttc aagcgcgaa attgacatg 
      tttttgaaa ttatcccac gaaaacatc acaaagatt 
      tacggactg tgtcgtgag gccgagccc cactacatg 
      atcctcgaa cacaccgat tggggcgat ttgaagcag 
      tttttggtg gcgacccag aaaggctcg ccgccgcca 
      ctgacccca gtgcagtgc gtggcaata gtacaccag 
      ctgtgtcaa ggtttggac catttggcc acttctcgc 
      ctgatccat agggattta gcggcgaga aattgtcta 
      gtcacttcc acgttaaaa gccaaagtt ggtcttccg 
      cgacttacg agggatccc tacagtcaa gaatactgc 
      aaacatgtg aaccacata attccgctg cggtggttg 
      ccctacgag gctgtttat gaggacgag tactcgaca 
      aagagcgac gtgtactcg tttgccgtc gtcatttgg 
      gagattttc aaccagggc gagctgccg ttccccaaa 
      atgaacgac acgagtttc ctaaataag ctcaaagag 
      aagaaactc gagtggaaa ccgcactct tccacgcct 
      gaatcttta caaaagtta caggcgtcc tgttgggac 
      agtaatccg caaaacagg ccgacgttc tccgaatta 
      gcgaaagaa atcgaagag gtacttaag tctatgtaa 
      atacgataa tttattaaa gagtcatct aaaccaaat 
      ttaggatac gaaattcaa taatcatgt aagcttcca 
      gtttatgaa agaattata taagcctta tgcaatgtt 
      gaggaaatt gactgtctc attgtgtgt actattgta 
      atattatta tttatgtta gggtaagtt cgatgagtt 
      tgatctcaa atctaaact ggctaaaga ggttataaa 
      attgtacat atcatttcg tttaagtct gtaggtagt 
      gtaaaaagc atatcatta aggtaattt taagttaat 
      gttagtgta tattgattg tcttgagcc acaaagcac 
      tgccaaata ctcttggag acaacgtaa tatacaatc 
      gagcgcata gactgtttt acaataaaa gctcccaat 
      aatgtaacg attctcgtc aatcatatt ccaatttta 
      acaagtgtt ctttgctca tagcttttt ataatgcac 
      ttttatata tagtggggt agtttacag tttgttttt 
      tctacttac tgtaggtct gtagacgaa ttcacaata 
      agtataata gcatgatat tcatatgta cgtaatagc 
      caataatgc ttcttaatt cagtcagtg taggaaaaa 
      gtctggaat ctttaaggg ctggaaaga ccagtgttt 
      tcgacaact ttttgagca acaactaag tgtttttat 
      gtttgtact taattcata aatgtacat tataaagaa 
      atattgtta attatcata atccactca aatgtgtga 
      ggactatta cacacttgt atgatacat gatctgtcc 
      atatttagt gatgtagct ttttataat gattttata 
      ctgtattgt ccattttta tataattat gcataatca 
      tctcttgtg atatgtatt ctaaattaa aaaatatat 
      accaaacat gctt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY