Status
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Gene information
General information
TC031843
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005139
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451544.1: 44..2997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031843
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06765

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031843 RA
      MDLHLPITG LQRRNTSNP VLELHTDSS RFANLGIST 
      VAVFLDIER AFDKVWHDG LILKLLSLQ INPRFVRLI 
      ASFLTNRSC SVKVQNALS NPIPIRSGV PQGSILSPL 
      LYIVYCRDF PISDHPRTK TRLFADDTA IWSSHKSPS 
      IAAKRIQTQ LQRIQQWTN TWRVKPNPT KSQSITMSY 
      KCSRRALSQ PIQLLLNNQ QIPHLKTIK YLGVTFSHT 
      CSLAPDINE TLKKVRNRA NLLYLIRGR LHGCSPQTL 
      LYTYNSFIR PVIEYRAPI YASIPLNQL LQIASTERR 
      ILRKIFRLD PSCKPSLKQ NSNCPQTKI SSPPYCSSS 
      VLLRFSPAN SPTTHRRNP TFIAITSQT TFIPPRPDC 
      QNQDHRSQN SPLPDSPPP GFTPTCDKR PNFKVVKKM 
      ARTKQTARK STGGKAPRK QLATKAARK SAPATGGVK 
      KPHRYRPGT VALREIRRY QKSTELLIR KLPFQRLVR 
      EIAQDFKTD LRFQSSAVM ALQEASEAY LVGLFEDTN 
      LCAIHAKRV TIMPKDIQL ARRIRGERA
    CDS sequence
    • >TC031843 RA
      atggatctc cacctgcca atcactgga ttacagaga 
      cgtaacacc tcaaatcca gtcctcgaa cttcacact 
      gactcctct cgttttgcc aaccttgga atcagcacg 
      gtcgctgtc tttttggac attgagcgt gcttttgac 
      aaggtctgg cacgacgga cttatccta aaactccta 
      agtctccaa attaacccc cgatttgtt cgcctcatc 
      gcctccttt cttactaac cgatcctgt agtgtcaaa 
      gttcaaaac gctttgtca aacccaatc cccattcgc 
      tcaggagtc ccccagggc tccattctt tccccctta 
      ctttacatt gtatactgc agagatttt ccaatttca 
      gatcacccg aggacaaag actagactc ttcgccgac 
      gacaccgca atctggtca tcccacaaa tctccctca 
      atagccgcc aaacgcatc caaacccaa ctccaacgc 
      atccaacag tggaccaac acctggcgc gtcaaaccc 
      aaccccaca aaatcccaa tctattacc atgtcgtac 
      aaatgctcc agacgtgcc ctctctcaa cccattcaa 
      cttctcctc aataatcaa caaattccc catctcaaa 
      accatcaaa tacctcgga gtcaccttc tcacacact 
      tgttcttta gcccccgac attaacgaa accctcaaa 
      aaagtccga aatcgcgcc aaccttctc tatctcatc 
      cgtggccgc cttcacggc tgcagcccc caaactctt 
      ctttacacc tacaactct ttcatccgc cccgtcatt 
      gaataccgt gccccaatc tatgcctct atccccctc 
      aaccaactt cttcaaatc gcctccacc gaacgccga 
      atccttcga aaaattttc cgacttgac cccagctgc 
      aaaccgtct cttaaacag aattccaat tgtccccaa 
      acaaaaata tcgtcaccc ccctactgc tcttcttca 
      gtcctccta cgattctct cccgccaat tctccaaca 
      actcatcga cgaaacccc actttcatt gcgataacc 
      tcccaaaca actttcatt ccaccccga ccagattgc 
      cagaaccag gaccatcgc tcccaaaac tctccactc 
      ccggactcg ccgccccca ggatttacg cccacttgc 
      gacaagcgc cctaatttt aaagtagtc aaaaaaatg 
      gcccgtacc aaacaaact gctcgtaaa tcgaccggt 
      ggaaaagct ccacgaaaa cagcttgcc actaaggca 
      gcaagaaaa agcgcaccc gccactgga ggagtgaaa 
      aaacctcat cgctacagg cccggtaca gtggcactg 
      cgtgaaatt cgtcgttat cagaaaagc accgaactg 
      ttgattcgt aaattgccc ttccaacgc ttggtcagg 
      gaaatagcc caggatttc aagaccgat cttcgtttc 
      caaagttct gccgttatg gcactccaa gaagcgagt 
      gaagcgtat cttgtcgga ctttttgaa gatacaaat 
      ttatgtgcc atccatgca aaacgcgtc accatcatg 
      cctaaagac atccaactg gcaagacgt attcgagga 
      gaacgagct taa
    mRNA sequence
    • >TC031843 RA
      cccccggac atcaaccgc caaatctcc gacctaacc 
      aaccgcgtc atttcagcc cgagacgcc gctattcca 
      acagccatc attcccaaa tttcgcccc agcattcct 
      cccgacatt ctcggcctc attcgagaa aaacgccga 
      atttttcga caatttctt cgcacccgt gacccattt 
      ctcaagacc caattcaac agactcaac gcccaaatc 
      cggagagac atcaatcaa caccgtgaa aaccaatgg 
      atctccacc tgccaatca ctggattac agagacgta 
      acacctcaa atccagtcc tcgaacttc acactgact 
      cctctcgtt ttgccaacc ttggaatca gcacggtcg 
      ctgtctttt tggacattg agcgtgctt ttgacaagg 
      tctggcacg acggactta tcctaaaac tcctaagtc 
      tccaaatta acccccgat ttgttcgcc tcatcgcct 
      cctttctta ctaaccgat cctgtagtg tcaaagttc 
      aaaacgctt tgtcaaacc caatcccca ttcgctcag 
      gagtccccc agggctcca ttctttccc ccttacttt 
      acattgtat actgcagag attttccaa tttcagatc 
      acccgagga caaagacta gactcttcg ccgacgaca 
      ccgcaatct ggtcatccc acaaatctc cctcaatag 
      ccgccaaac gcatccaaa cccaactcc aacgcatcc 
      aacagtgga ccaacacct ggcgcgtca aacccaacc 
      ccacaaaat cccaatcta ttaccatgt cgtacaaat 
      gctccagac gtgccctct ctcaaccca ttcaacttc 
      tcctcaata atcaacaaa ttccccatc tcaaaacca 
      tcaaatacc tcggagtca ccttctcac acacttgtt 
      ctttagccc ccgacatta acgaaaccc tcaaaaaag 
      tccgaaatc gcgccaacc ttctctatc tcatccgtg 
      gccgccttc acggctgca gcccccaaa ctcttcttt 
      acacctaca actctttca tccgccccg tcattgaat 
      accgtgccc caatctatg cctctatcc ccctcaacc 
      aacttcttc aaatcgcct ccaccgaac gccgaatcc 
      ttcgaaaaa ttttccgac ttgacccca gctgcaaac 
      cgtctctta aacagaatt ccaattgtc cccaaacaa 
      aaatatcgt cacccccct actgctctt cttcagtcc 
      tcctacgat tctctcccg ccaattctc caacaactc 
      atcgacgaa accccactt tcattgcga taacctccc 
      aaacaactt tcattccac cccgaccag attgccaga 
      accaggacc atcgctccc aaaactctc cactcccgg 
      actcgccgc ccccaggat ttacgccca cttgcgaca 
      agcgcccta attttaaag tagtcaaaa aaatggccc 
      gtaccaaac aaactgctc gtaaatcga ccggtggaa 
      aagctccac gaaaacagc ttgccacta aggcagcaa 
      gaaaaagcg cacccgcca ctggaggag tgaaaaaac 
      ctcatcgct acaggcccg gtacagtgg cactgcgtg 
      aaattcgtc gttatcaga aaagcaccg aactgttga 
      ttcgtaaat tgcccttcc aacgcttgg tcagggaaa 
      tagcccagg atttcaaga ccgatcttc gtttccaaa 
      gttctgccg ttatggcac tccaagaag cgagtgaag 
      cgtatcttg tcggacttt ttgaagata caaatttat 
      gtgccatcc atgcaaaac gcgtcacca tcatgccta 
      aagacatcc aactggcaa gacgtattc gaggagaac 
      gagcttaaa atgaaaaaa taaacaaaa aaaaaacgg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY