Status
Please wait ...
Gene information
General information
TC015524 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1726172..1734347
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015524
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 7

    wings shortened - Number of animals showing the phenotype: 7

    legs slightly shortened - Number of animals showing the phenotype: 7
    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 4

    antenna shortened - Number of animals showing the phenotype: 4


    flagellomeres partially fused - Number of animals showing the phenotype: 4

    prothorax slightly size decreased - Number of animals showing the phenotype: 4

    prosternal process shortened - Number of animals showing the phenotype: 4


    legs slightly shortened - Number of animals showing the phenotype: 3


    elytra shortened - Number of animals showing the phenotype: 4


    hindwing folding irregular - Number of animals showing the phenotype: 4

    elytra dorsal not closed - Number of animals showing the phenotype: 4


    elytra surface irregular - Number of animals showing the phenotype: 4

    elytra distal colour lighter - Number of animals showing the phenotype: 3

    Fertility
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_02486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015524 RA
      MSLKPRRVD FNATWGSIR DTIRGVITL DHVPRPVWN 
      DRFSDVYSL CVAHPEPMA DRLYAETKQ YLIDHVSKL 
      LVQVQDEGE ANLVKSYYH YWSQYSVGS QYLHSLYLY 
      LNQQHIRTQ KLSDAEIIY GSSDSSGGE QMEIGELAL 
      EVWQSGMIA PLGQRLVKL LLEAIGEDR AQNTCAIPI 
      DAVRGTILS FVEVQGYKK KGQLQLYQE LFEEPFLKE 
      SGEHFKRDA AKLLQEKNV SLYMERVKG RIEEELLRA 
      RRFLHTSSL PKVSQRCET HMVAEHLDF LYSECVAMV 
      QGERKKDLS NMYDLLKSV QNAMVVLVD TVLDHIKTQ 
      GLTAVGNID TENIHITFV ENMLAVYKK YKLLIQEVF 
      KSDQNFMGA LDKACSSVI NHKFGKPVC KSPEFLAKY 
      CDALLRKSS KGISDSEVD EKLAESITI FKYIDDKDV 
      FQKFYSRML AKRLIHQQT QSMDAEEAM INRLKQACG 
      YEFTSKLHR MFTDMSVSA DLNNKFNAF LKQDNIDLG 
      INFSIYVLQ AGAWPLGQT VTTPFTYPR QLEKSVQMF 
      ENFYHNRFN GRKLTWLHN LCQAELKLG HLKKSYLVT 
      MQTFQMATL LLFEHTDSL TFREIRESL QLTAEQYHR 
      YAASLIDCK LLIADSTDL KPETVLRLN MEYNNKRTK 
      FRINAAVQK ESPQEIEQT MNSVEEDRK MYLQAAIVR 
      IMKSRKILK HNALIQEVY AQSKVSFAP SVQLIKKCI 
      ESLIDKQYI ERTPHSSEE YSYVA
    CDS sequence
    • >TC015524 RA
      atgtctctg aaaccgcgc cgtgtggac ttcaacgcg 
      acctggggc tccatccgg gacaccatc cggggggtg 
      atcaccctg gaccacgtc ccgcgcccc gtgtggaac 
      gaccgcttc tcggacgtc tactcgcta tgtgtggcc 
      catcccgag cccatggcg gaccgtttg tacgccgag 
      acgaagcag tacctcatc gaccacgtc tccaaactg 
      cttgtgcaa gtgcaggac gagggcgag gccaatttg 
      gtgaaaagt tactaccat tattggtcg cagtactcg 
      gtagggagt cagtacttg cacagtttg tacttgtac 
      ctcaaccag caacatata aggactcag aaactgtcc 
      gatgctgag attatttac gggagttcg gattcgtca 
      gggggcgaa caaatggag atcggggag ttggcgctg 
      gaggtgtgg cagtcgggg atgatagcc cccttgggg 
      caaaggctt gttaaactg ctactggag gcgattggg 
      gaggatagg gcgcaaaat acgtgtgct ataccgatt 
      gatgcagtc aggggcaca attcttagt tttgttgag 
      gtgcagggc tacaagaaa aagggccaa ctgcaactg 
      taccaggag ttatttgag gagccgttc ctcaaagaa 
      agcggcgag cattttaaa cgcgacgct gccaaactt 
      ttgcaagag aaaaacgtc tccctgtat atggagcgc 
      gtcaaggga cgaattgag gaggaactg ttacgtgca 
      cgtcgcttc ctccacacc agttccctc ccgaaagtc 
      tcccaacga tgtgaaaca cacatggtg gccgaacat 
      ttggacttt ttatacagc gaatgcgtg gcaatggta 
      cagggtgag cgcaaaaag gacctctca aatatgtac 
      gatctgctc aaaagcgtc caaaatgca atggttgtg 
      cttgttgat accgttctg gaccatatt aagacgcaa 
      ggattgacg gctgttggt aatattgat accgaaaat 
      attcacata acgttcgtc gaaaatatg ctagcggtt 
      tataaaaaa tataaattg ttgattcaa gaggtgttc 
      aagtcggat cagaatttt atgggggct ttggataag 
      gcctgcagt tccgttatt aatcacaag tttggcaag 
      cctgtgtgt aaaagcccc gaatttttg gccaagtat 
      tgcgacgca ttgttgcgc aagtcgagt aaagggatt 
      agcgattcg gaggtggac gaaaagctc gccgagagc 
      ataacaatt ttcaaatat atcgacgac aaagacgta 
      tttcaaaaa ttctattct cgaatgttg gccaaacgc 
      ctcatccac caacaaacg caaagtatg gatgcggag 
      gaggccatg atcaatcgt ttaaaacaa gcatgcggt 
      tacgaattt acaagtaaa ttgcatcga atgtttacc 
      gatatgtca gtgagtgcg gatctcaat aataaattt 
      aacgcattt ttgaaacaa gacaacatc gacctcgga 
      attaatttc agtatatac gtattacaa gcaggggcg 
      tggccattg ggccagacg gtgaccacg ccttttacg 
      tatcctaga caactggag aagagcgta caaatgttt 
      gaaaatttc tatcataat aggttcaac ggtcgcaaa 
      ttgacatgg ttgcataat ttgtgtcaa gcggaattg 
      aagttgggc catttgaaa aaatcgtat ctagtgaca 
      atgcagacg tttcaaatg gccactttg ttgctgttt 
      gaacacacg gattcgctc acttttcgc gaaattagg 
      gagagtttg caactaacc gccgaacag tatcacaga 
      tacgcggct agcttgatt gattgtaaa ttattgata 
      gcggatagt acggatttg aaaccggaa acggtgctc 
      cgtttaaac atggaatat aacaacaag aggacgaag 
      ttccggata aatgcggct gtgcagaaa gagtcgcct 
      caggagatt gaacaaacg atgaattcg gtcgaagag 
      gaccggaag atgtacttg caggcggcc attgttagg 
      attatgaag tcgaggaaa atactcaaa cataacgct 
      ttgatacaa gaggtttat gcgcaatcg aaagtttcc 
      ttcgcaccg agtgttcag ttgatcaaa aagtgcatc 
      gaatctctc attgataaa cagtatatc gaaaggacg 
      ccacattcg agcgaggag tacagttat gtagcttag
    mRNA sequence
    • >TC015524 RA
      cattgttgt caaaactcc aagtcatcg aagcatgcg 
      tgttttacc accaacgtg actcgattt tttcgcaac 
      gataaaacc aaaaaatca gttaacgag gtacgaagg 
      cgcctaatt ggggccaaa tgccccgaa ttgagcccc 
      tttttgaag cttcctaac ctcactaaa acgtccaaa 
      ccccccttt cgaaaattc caagatgga gtcacccaa 
      ctgacattc atacgtcaa aaaactgac cccaatttt 
      cgtcgtagt gtctccaat aataatgag aaattcacc 
      gcgattata gaaacgtaa acgtccgaa aaaaccgtt 
      ttattttta gaaccgctc agtcatgtc tctgaaacc 
      gcgccgtgt ggacttcaa cgcgacctg gggctccat 
      ccgggacac catccgggg ggtgatcac cctggacca 
      cgtcccgcg ccccgtgtg gaacgaccg cttctcgga 
      cgtctactc gctatgtgt ggcccatcc cgagcccat 
      ggcggaccg tttgtacgc cgagacgaa gcagtacct 
      catcgacca cgtctccaa actgcttgt gcaagtgca 
      ggacgaggg cgaggccaa tttggtgaa aagttacta 
      ccattattg gtcgcagta ctcggtagg gagtcagta 
      cttgcacag tttgtactt gtacctcaa ccagcaaca 
      tataaggac tcagaaact gtccgatgc tgagattat 
      ttacgggag ttcggattc gtcaggggg cgaacaaat 
      ggagatcgg ggagttggc gctggaggt gtggcagtc 
      ggggatgat agccccctt ggggcaaag gcttgttaa 
      actgctact ggaggcgat tggggagga tagggcgca 
      aaatacgtg tgctatacc gattgatgc agtcagggg 
      cacaattct tagttttgt tgaggtgca gggctacaa 
      gaaaaaggg ccaactgca actgtacca ggagttatt 
      tgaggagcc gttcctcaa agaaagcgg cgagcattt 
      taaacgcga cgctgccaa acttttgca agagaaaaa 
      cgtctccct gtatatgga gcgcgtcaa gggacgaat 
      tgaggagga actgttacg tgcacgtcg cttcctcca 
      caccagttc cctcccgaa agtctccca acgatgtga 
      aacacacat ggtggccga acatttgga ctttttata 
      cagcgaatg cgtggcaat ggtacaggg tgagcgcaa 
      aaaggacct ctcaaatat gtacgatct gctcaaaag 
      cgtccaaaa tgcaatggt tgtgcttgt tgataccgt 
      tctggacca tattaagac gcaaggatt gacggctgt 
      tggtaatat tgataccga aaatattca cataacgtt 
      cgtcgaaaa tatgctagc ggtttataa aaaatataa 
      attgttgat tcaagaggt gttcaagtc ggatcagaa 
      ttttatggg ggctttgga taaggcctg cagttccgt 
      tattaatca caagtttgg caagcctgt gtgtaaaag 
      ccccgaatt tttggccaa gtattgcga cgcattgtt 
      gcgcaagtc gagtaaagg gattagcga ttcggaggt 
      ggacgaaaa gctcgccga gagcataac aattttcaa 
      atatatcga cgacaaaga cgtatttca aaaattcta 
      ttctcgaat gttggccaa acgcctcat ccaccaaca 
      aacgcaaag tatggatgc ggaggaggc catgatcaa 
      tcgtttaaa acaagcatg cggttacga atttacaag 
      taaattgca tcgaatgtt taccgatat gtcagtgag 
      tgcggatct caataataa atttaacgc atttttgaa 
      acaagacaa catcgacct cggaattaa tttcagtat 
      atacgtatt acaagcagg ggcgtggcc attgggcca 
      gacggtgac cacgccttt tacgtatcc tagacaact 
      ggagaagag cgtacaaat gtttgaaaa tttctatca 
      taataggtt caacggtcg caaattgac atggttgca 
      taatttgtg tcaagcgga attgaagtt gggccattt 
      gaaaaaatc gtatctagt gacaatgca gacgtttca 
      aatggccac tttgttgct gtttgaaca cacggattc 
      gctcacttt tcgcgaaat tagggagag tttgcaact 
      aaccgccga acagtatca cagatacgc ggctagctt 
      gattgattg taaattatt gatagcgga tagtacgga 
      tttgaaacc ggaaacggt gctccgttt aaacatgga 
      atataacaa caagaggac gaagttccg gataaatgc 
      ggctgtgca gaaagagtc gcctcagga gattgaaca 
      aacgatgaa ttcggtcga agaggaccg gaagatgta 
      cttgcaggc ggccattgt taggattat gaagtcgag 
      gaaaatact caaacataa cgctttgat acaagaggt 
      ttatgcgca atcgaaagt ttccttcgc accgagtgt 
      tcagttgat caaaaagtg catcgaatc tctcattga 
      taaacagta tatcgaaag gacgccaca ttcgagcga 
      ggagtacag ttatgtagc ttagaatca ctaacttat 
      gtttgatcg gtttgataa atattgtaa catatttgt 
      aaataagtg atcacttta atttttcta tggtttcat 
      tctggccag gttagttta cgactaatt actgtaatt 
      tgccctttg tgataataa aatataaac aagagt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY