Status
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Gene information
General information
TC015317 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 561721..570779
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015317
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02453

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    iBeetle screen iB_02453

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015317 RA
      MASIEYDLD TSGGLMDQI QALIAPPQS EDTKSRKGA 
      TATEHPYFK RPGKGHNHD SCDACGEGG DLICCDKCP 
      SSFHLQCHD PPLEEKDIP SGEWLCHSC KYAKKSNAP 
      QTRNKRSAS TPAGKAPKK AKVSPMDLL IQAASSMNP 
      KQFDLPRSM SVPCIFPGT DKVEAPYSR NGRKSMKVH 
      KDHEKKQDA TIFLPAKKC HECRKSCRV APLISCDFC 
      SLYFHMDCL DPPLTTPPS GRWMCPNHV EHYLDSKLL 
      TSISATERI KLWDKFNGP VDQDAIRLE FFRKIHSKN 
      PPFRVKIKL ASKNRVKVP AMVKYHYRK PVKLLPSLK 
      DVLRLESVL NNREQMVED AVKCEDVEV VQSLCNGPE 
      DQTCDKSTD ENTNNTQED DTKEDFCRN GCIKNEVMN 
      GATVDQKDN IFLPDVVSD ITNEVEVEL KQLDDRLVK 
      LLAYQRIQH LLNQPNNSA NLNLYLPSN LQSKLRHMP 
      MPSELLTPA DIERISRVF SSPKKKIKP KSALRARAM 
      MCPVVSPHF YNVRTSEVS PNDVRHDDS FLGFRPTVS 
      ARYPEAVAM RYRVLNVGK GSSNDVDLE RFGHCNNIS 
      ARHAIIFYD EFTKNFELI NYSSHGTFV NNVLFSNDT 
      NTERPKQCE GKIVPLEVQ VREIIDRRR KVVRQRKNS 
      FEAKMTAID NMDTMECCC TNVGESSSR IGWEGSAIL 
      NHGSLLRFG CVSFVFSIV DCAA
    CDS sequence
    • >TC015317 RA
      atggcttca atcgagtac gatttggac acatctggg 
      ggtctcatg gaccaaatc caggctctg atcgcgccc 
      ccgcagtcc gaggacacg aaatcgcgc aagggggcc 
      accgccacg gagcacccg tacttcaag cggccggga 
      aaggggcac aaccacgac agttgcgac gcgtgcggc 
      gaagggggc gatttgatt tgctgtgac aagtgtccg 
      tcgagtttt catctgcag tgccatgat cccccattg 
      gaggagaag gatataccg agtggagag tggttgtgt 
      cacagttgt aaatatgca aaaaagagc aatgcgccc 
      caaacgagg aacaaacgc tcggcgtcc acgccggcg 
      gggaaagcc cccaaaaag gcgaaagtc agtccgatg 
      gatttgtta attcaggct gcgagttca atgaatccc 
      aagcagttt gatttgccc aggagtatg agtgtaccg 
      tgcattttt ccagggact gataaagtc gaagcccca 
      tattctcgc aacggccgc aaaagcatg aaagtccac 
      aaagaccac gagaaaaaa caagatgct acaattttc 
      cttccagca aagaaatgc cacgaatgt cgcaaatct 
      tgccgtgta gctccgcta atatcgtgc gacttttgc 
      tccctatac tttcacatg gattgctta gatccaccg 
      ctaacaacg ccacctagc ggccgctgg atgtgcccg 
      aatcacgtc gaacactat ctcgactct aaactgttg 
      acttcgatt tcggccacc gaacgcatc aaactgtgg 
      gataaattc aacggtccc gtcgatcaa gacgccata 
      cgtttggag ttttttcgt aaaattcac agtaaaaat 
      ccgccgttt cgtgtaaaa attaaattg gcgtcgaaa 
      aatcgcgtt aaagtaccg gcaatggtc aaatatcat 
      tatcgtaaa ccggtgaaa ttgttgccg agtttgaag 
      gatgtcttg cggttagag agtgtgtta aacaatcga 
      gagcagatg gttgaggac gctgttaag tgtgaagat 
      gttgaagtt gtacaaagt ttgtgtaac ggtccggag 
      gatcaaacg tgtgataaa agtacagat gaaaatacg 
      aataatact caagaggac gacacaaag gaggatttt 
      tgtcgaaat ggttgtatt aaaaatgag gtaatgaat 
      ggggcaacg gttgaccaa aaggacaat atttttttg 
      ccggacgtc gtctcggat attacgaac gaagttgaa 
      gtcgaattg aagcaatta gacgaccga ttggtgaaa 
      ctcctcgct tatcagcgc atccagcac ctgctcaac 
      cagccgaac aattccgcc aacttaaat ttgtatcta 
      ccgtcgaat ttgcagagc aaattacgt cacatgcct 
      atgccgagt gaattgttg acgcctgcc gatatcgaa 
      cgaatatcg cgcgttttt tccagtccg aagaagaaa 
      atcaagccg aagtcggcg cttcgcgca cgtgctatg 
      atgtgtccg gtggtttcc ccacatttc tacaatgtg 
      cgcacgtcc gaagtcagt ccgaacgat gtgcgacac 
      gacgatagt tttctcgga tttaggccg acggtttcg 
      gcgagatat cccgaagcg gtggcgatg agataccgg 
      gtgttgaat gtggggaaa ggatcgtcc aatgatgtc 
      gatttggaa cggtttgga cattgcaat aacatatcg 
      gcaaggcat gcgatcatt ttctacgat gagttcacg 
      aaaaacttc gagctgatc aactactcg tcccacggt 
      acatttgtg aacaacgtg ctgttttca aacgacacc 
      aataccgaa cgccccaaa cagtgtgaa ggaaaaatc 
      gttccgttg gaagtccaa gtgcgtgaa atcattgat 
      agaagacga aaagtagtc cgccaacga aaaaactcg 
      tttgaagcg aaaatgaca gcaatagat aacatggat 
      acgatggaa tgttgttgt acaaatgtt ggtgaatca 
      tcatcacgt ataggatgg gaaggatcg gcaatttta 
      aaccacgga agtctttta aggttcgga tgtgtctcg 
      ttcgtattt tcgatcgtc gattgtgcg gcataa
    mRNA sequence
    • >TC015317 RA
      cagctcgcg gcaaacact gtcaaaaac ctgccaaac 
      gttttataa ataagcgta aaaattcaa gtgttatta 
      atgaattta tctcaaccg attgatcgt ttcgccccc 
      atttcaccg tcatcataa ccaaattgc agttttcag 
      tgccattca tattttccc ccaaccatg taaccaaaa 
      ccgaaaaaa ccaaactcg agtgagtga aaaaccact 
      cagttcatt cccacgcca ccgttctcc tcagcaatg 
      gcttcaatc gagtacgat ttggacaca tctgggggt 
      ctcatggac caaatccag gctctgatc gcgcccccg 
      cagtccgag gacacgaaa tcgcgcaag ggggccacc 
      gccacggag cacccgtac ttcaagcgg ccgggaaag 
      gggcacaac cacgacagt tgcgacgcg tgcggcgaa 
      gggggcgat ttgatttgc tgtgacaag tgtccgtcg 
      agttttcat ctgcagtgc catgatccc ccattggag 
      gagaaggat ataccgagt ggagagtgg ttgtgtcac 
      agttgtaaa tatgcaaaa aagagcaat gcgccccaa 
      acgaggaac aaacgctcg gcgtccacg ccggcgggg 
      aaagccccc aaaaaggcg aaagtcagt ccgatggat 
      ttgttaatt caggctgcg agttcaatg aatcccaag 
      cagtttgat ttgcccagg agtatgagt gtaccgtgc 
      atttttcca gggactgat aaagtcgaa gccccatat 
      tctcgcaac ggccgcaaa agcatgaaa gtccacaaa 
      gaccacgag aaaaaacaa gatgctaca attttcctt 
      ccagcaaag aaatgccac gaatgtcgc aaatcttgc 
      cgtgtagct ccgctaata tcgtgcgac ttttgctcc 
      ctatacttt cacatggat tgcttagat ccaccgcta 
      acaacgcca cctagcggc cgctggatg tgcccgaat 
      cacgtcgaa cactatctc gactctaaa ctgttgact 
      tcgatttcg gccaccgaa cgcatcaaa ctgtgggat 
      aaattcaac ggtcccgtc gatcaagac gccatacgt 
      ttggagttt tttcgtaaa attcacagt aaaaatccg 
      ccgtttcgt gtaaaaatt aaattggcg tcgaaaaat 
      cgcgttaaa gtaccggca atggtcaaa tatcattat 
      cgtaaaccg gtgaaattg ttgccgagt ttgaaggat 
      gtcttgcgg ttagagagt gtgttaaac aatcgagag 
      cagatggtt gaggacgct gttaagtgt gaagatgtt 
      gaagttgta caaagtttg tgtaacggt ccggaggat 
      caaacgtgt gataaaagt acagatgaa aatacgaat 
      aatactcaa gaggacgac acaaaggag gatttttgt 
      cgaaatggt tgtattaaa aatgaggta atgaatggg 
      gcaacggtt gaccaaaag gacaatatt tttttgccg 
      gacgtcgtc tcggatatt acgaacgaa gttgaagtc 
      gaattgaag caattagac gaccgattg gtgaaactc 
      ctcgcttat cagcgcatc cagcacctg ctcaaccag 
      ccgaacaat tccgccaac ttaaatttg tatctaccg 
      tcgaatttg cagagcaaa ttacgtcac atgcctatg 
      ccgagtgaa ttgttgacg cctgccgat atcgaacga 
      atatcgcgc gttttttcc agtccgaag aagaaaatc 
      aagccgaag tcggcgctt cgcgcacgt gctatgatg 
      tgtccggtg gtttcccca catttctac aatgtgcgc 
      acgtccgaa gtcagtccg aacgatgtg cgacacgac 
      gatagtttt ctcggattt aggccgacg gtttcggcg 
      agatatccc gaagcggtg gcgatgaga taccgggtg 
      ttgaatgtg gggaaagga tcgtccaat gatgtcgat 
      ttggaacgg tttggacat tgcaataac atatcggca 
      aggcatgcg atcattttc tacgatgag ttcacgaaa 
      aacttcgag ctgatcaac tactcgtcc cacggtaca 
      tttgtgaac aacgtgctg ttttcaaac gacaccaat 
      accgaacgc cccaaacag tgtgaagga aaaatcgtt 
      ccgttggaa gtccaagtg cgtgaaatc attgataga 
      agacgaaaa gtagtccgc caacgaaaa aactcgttt 
      gaagcgaaa atgacagca atagataac atggatacg 
      atggaatgt tgttgtaca aatgttggt gaatcatca 
      tcacgtata ggatgggaa ggatcggca attttaaac 
      cacggaagt cttttaagg ttcggatgt gtctcgttc 
      gtattttcg atcgtcgat tgtgcggca taaaaaact 
      cactcacaa caaagatac atttttact tgatgattt 
      gaatacaac atacatgat aca

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY