Status
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Gene information
General information
TC015063 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3894855..3902092
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015063
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015063 RA
      MSATAQTNG KKSSNSSIV NTPASTPVT NGNSQTISQ 
STPPPPNNN APKYGTLVP NRIFVGGIS ANTTEGELL 
QLFSSYGTV KAAKIIQDR AGVSKGYGF ITFESEDDA 
KRPLKDADN IVLKERKLN IAPAIKKQP FNRAFDAAS 
PSTVAPGNT AQYFFPPSP GVPYFQGGV TYYQQPPAA 
PGDPNSQTT VYQAPPVYP AQTGQPQAP TYPPMMFPH 
HIYMPQQYA MPPMPYDYN YYPNNGGGA QYMVGGQNG 
GPGGGGSGG NSQGGPPMP PPNSPPRPP CYNAQLPPY 
TPDPVYYNM PVYGGPVEG APLYPEGLD MSNGVGYPE 
VSQLLGEFL HGFDGCTDI HQGPYPMAD PTQDINDSA 
PPPLPPPIA TPLTADHAP LYEHETNDA DNSRQSKTP 
VVSLLSMEQ HQEKDFVSM QGGRRVKPQ PPPPPPPQQ 
QPMIIPHFA QNQAPPPIY YNFNGPPVR SHQIPPYNN 
NNIKTTNGY RKPRTFNSA SANRANDNS SRSSLRTDS 
NSSANAAVD ENKNEDKQS PNSFHLYNG SRTYQNSLG 
RRINNNKKD TALDNNNKT KSNFVVQSQ QQLRNNGQQ 
SYVLTSASA ASVVRRNKR STRRNVGQS GGSLSEIGA 
GDAPLANND VTDTCKKME TLKM
    CDS sequence
    • >TC015063 RA
      atgtcggcc accgctcaa accaacggc aagaagagc 
tcgaacagt tccattgtg aacacccca gccagcact 
ccggtgact aacggcaat tcgcaaacg atctcccag 
agcacccct ccgcccccg aacaacaac gcccccaag 
tacggcacc ctggtcccc aaccgaatc ttcgtcggc 
ggcatctcc gccaacacc accgaaggc gagctcttg 
cagctcttc agcagttac gggaccgtc aaagcggcc 
aaaatcatc caagacagg gcaggcgtc tccaaaggc 
tacgggttc ataacgttc gaaagcgaa gacgatgcc 
aaacgcccc ctcaaagac gccgacaac atcgtcctc 
aaggaaaga aaactgaac attgcgcca gcaatcaag 
aaacaaccg tttaatcgg gcctttgac gccgccagt 
ccctcgact gtcgcgcca gggaacacg gcacagtac 
tttttcccg ccgagcccc ggagtgccc tactttcaa 
gggggcgtc acttactac caacagcca ccggcagcc 
cccggcgat cccaactca caaacgacc gtctatcag 
gctcctcct gtatatccg gcccaaacc ggacaacca 
caagcccca acttaccca ccgatgatg ttccctcat 
cacatctat atgccacaa cagtacgcc atgccaccg 
atgccgtac gactacaac tactaccct aacaacgga 
ggaggagcc cagtacatg gtcggcggt cagaatggg 
ggcccagga ggcggcgga tccggcgga aattctcag 
ggcggcccc ccaatgccc cccccgaac agcccccct 
cgaccgcct tgctacaac gcccaattg cccccgtac 
acaccggac ccagtatac tacaacatg ccagtatac 
ggcggtccg gtcgagggg gcccccctc tatccagaa 
ggtctggac atgtccaac ggcgtcggt tatcctgag 
gttagtcag ttattaggc gagtttttg cacggcttt 
gacggatgc acggatatt catcagggc ccctatcct 
atggccgac cccacccaa gacataaac gactccgcc 
ccaccccca ctaccacca ccaatcgcc acacctctc 
accgccgac cacgcccca ctatacgaa cacgaaacg 
aacgacgcc gacaacagc cgacaaagc aaaacgccg 
gtcgtgtcg ctgctgtcg atggaacaa caccaagag 
aaagacttc gtttcgatg caaggcgga cgccgcgtc 
aaaccccaa ccaccccca ccaccaccg ccacaacaa 
caaccaatg attataccg catttcgcc caaaatcaa 
gccccgccc cccatctac tacaacttc aacggaccg 
cccgtacga tctcatcaa atcccgcct tacaacaac 
aacaacatt aaaacgacg aacggttat cgcaaaccg 
cgcactttt aactccgcc tccgcgaat cgagccaat 
gacaattcg tcgagatca agtctccgg actgattcg 
aattcgtcg gcgaacgcc gccgtcgac gaaaacaaa 
aacgaagat aaacaatcg ccaaacagt tttcatttg 
tataacggt tcgagaacg tatcagaat tcgctagga 
cgacgcatc aacaacaac aagaaggac accgccttg 
gacaacaac aacaaaact aaaagtaat ttcgttgtt 
caatcgcag caacagctg aggaacaac ggccagcag 
agctacgtg ctgacgtcg gcgtcggca gcgtcggtc 
gtcaggcgg aacaaaaga tcgacgcgt cgcaacgtc 
ggccaatcg ggaggttcg ctgagcgaa atcggcgcc 
ggtgacgcc cccctagcc aacaacgat gttacagat 
acctgtaaa aaaatggaa acactgaaa atgtga
    mRNA sequence
    • >TC015063 RA
      tcgacgcca ttttatcga tggctacgt tttgtagtg 
aaaacgact ttagttttt aagaatttc tgtgttcat 
ttctctaaa ttcactcat gtttcttca taaatttac 
ggtgttgtt gccacaagt agttttcct cagtgagtt 
tgcttggta atcggtaaa atgtcggcc accgctcaa 
accaacggc aagaagagc tcgaacagt tccattgtg 
aacacccca gccagcact ccggtgact aacggcaat 
tcgcaaacg atctcccag agcacccct ccgcccccg 
aacaacaac gcccccaag tacggcacc ctggtcccc 
aaccgaatc ttcgtcggc ggcatctcc gccaacacc 
accgaaggc gagctcttg cagctcttc agcagttac 
gggaccgtc aaagcggcc aaaatcatc caagacagg 
gcaggcgtc tccaaaggc tacgggttc ataacgttc 
gaaagcgaa gacgatgcc aaacgcccc ctcaaagac 
gccgacaac atcgtcctc aaggaaaga aaactgaac 
attgcgcca gcaatcaag aaacaaccg tttaatcgg 
gcctttgac gccgccagt ccctcgact gtcgcgcca 
gggaacacg gcacagtac tttttcccg ccgagcccc 
ggagtgccc tactttcaa gggggcgtc acttactac 
caacagcca ccggcagcc cccggcgat cccaactca 
caaacgacc gtctatcag gctcctcct gtatatccg 
gcccaaacc ggacaacca caagcccca acttaccca 
ccgatgatg ttccctcat cacatctat atgccacaa 
cagtacgcc atgccaccg atgccgtac gactacaac 
tactaccct aacaacgga ggaggagcc cagtacatg 
gtcggcggt cagaatggg ggcccagga ggcggcgga 
tccggcgga aattctcag ggcggcccc ccaatgccc 
cccccgaac agcccccct cgaccgcct tgctacaac 
gcccaattg cccccgtac acaccggac ccagtatac 
tacaacatg ccagtatac ggcggtccg gtcgagggg 
gcccccctc tatccagaa ggtctggac atgtccaac 
ggcgtcggt tatcctgag gttagtcag ttattaggc 
gagtttttg cacggcttt gacggatgc acggatatt 
catcagggc ccctatcct atggccgac cccacccaa 
gacataaac gactccgcc ccaccccca ctaccacca 
ccaatcgcc acacctctc accgccgac cacgcccca 
ctatacgaa cacgaaacg aacgacgcc gacaacagc 
cgacaaagc aaaacgccg gtcgtgtcg ctgctgtcg 
atggaacaa caccaagag aaagacttc gtttcgatg 
caaggcgga cgccgcgtc aaaccccaa ccaccccca 
ccaccaccg ccacaacaa caaccaatg attataccg 
catttcgcc caaaatcaa gccccgccc cccatctac 
tacaacttc aacggaccg cccgtacga tctcatcaa 
atcccgcct tacaacaac aacaacatt aaaacgacg 
aacggttat cgcaaaccg cgcactttt aactccgcc 
tccgcgaat cgagccaat gacaattcg tcgagatca 
agtctccgg actgattcg aattcgtcg gcgaacgcc 
gccgtcgac gaaaacaaa aacgaagat aaacaatcg 
ccaaacagt tttcatttg tataacggt tcgagaacg 
tatcagaat tcgctagga cgacgcatc aacaacaac 
aagaaggac accgccttg gacaacaac aacaaaact 
aaaagtaat ttcgttgtt caatcgcag caacagctg 
aggaacaac ggccagcag agctacgtg ctgacgtcg 
gcgtcggca gcgtcggtc gtcaggcgg aacaaaaga 
tcgacgcgt cgcaacgtc ggccaatcg ggaggttcg 
ctgagcgaa atcggcgcc ggtgacgcc cccctagcc 
aacaacgat gttacagat acctgtaaa aaaatggaa 
acactgaaa atgtgatta aaacgaggt ttgttgttg 
acacttaag aaagggata atttattta ttaaaaaag 
aaatgtttg cttgtatat aaaaaacac aaaaaaata 
ataataaaa atgcaatat tatttaaat aatcgacaa 
tattattgt tattaatac aaaagtgag atgaagccc 
gtttagcat tggacttta ctattatga ttcctagct 
taggttctt ttgtagata aagaagaga gaaaaaact 
aagtcctac acatatttt taacaagta attgttatt 
taatgtcta aggatgtgt ataaattag tttagtttc 
gattatttt tttctaaac tcgatgact cttattcgc 
aacaatcgt attttagtc gaggggaac tgtgtccta 
tcttgaatt tgaggtgtt gtttgctcg atgccttat 
ttcctctca acttgtacg ttctatagg ttagttttt 
gatattgtt tttcccctt tgaaaacag tctttacaa 
ttgacttta aaatcaaag tgagatttt tgctcaaaa 
aggctgcca tcggttttt agtccaatt cgagtgaat 
ctatcggct ctctaaatc acacaacac gcacttttg 
ttgtaattc cctcccatt ttgttttgt tgtttgtcg 
gaatatttc aattttagg ctgtgacgt taaattgtt 
ataaatttg tagtgattt catcgaaat tcaaaattt 
tttacggga cagattgtt acaaatcaa actattatt 
attacacaa aatgtttca aaagagcct aaaatttat 
tatgaatga tttttttcc aaagaagta ttttaggat 
tagtcgtaa ggattgtag caatgtaag atagtaatt 
tattaagta gtgtagata tttaggtgt catagttca 
taaatcgag aattttttg gccaaaact aattacact 
atttagtta attgtgctt accgttgcg ggtaagcaa 
ccgagtctt gttgaaagt atattaaca aagtacctg 
tattattaa atctacgct aaaatgaaa caaacaaaa 
catttgtaa atacctatt tagttgata tacttgttg 
atgtttgga aattttatc gaagaaagc gttttcgtt 
gcgttattg gccaacgta gcttttaat ttataagta 
ggtttaggg aagatcacc catgccccg cccctcaca 
aaaacaaga gcaatatgt aaactctgt caattttag 
tttgtatat ataataatc aaaaaactt gtcaaaaaa 
aagaaatgc tctaaaaac ggttgtgca taaaattcg 
tagtcgtaa agtatttag ctgtagttt aaatacacg 
attgatcgg ctcccaaaa cgccgaata caaaaatac 
aaactgaaa tgtgagcta tgataagcc aaatttttg 
tgatagcgg cgaaagatg tgtttttaa aattcaaat 
tttcccgcc tggggaaaa ttcgaattt taaaacaca 
aatttccga cttaaaatg tgtgttcaa caacttgaa 
atcttgtag gcatattat tagacttgc ttcgggagg 
ctctgcggc ctcgtctca atagctgta acaaacaac 
aaaaacgga aaaatagcg ttatggaac tatgaatgt 
aaaataaaa cgcaatatt ttagaaatt atataactt 
aagaaaaca gtgagaaat atttattaa ttacaataa 
caattataa ttaattaac tattaaata agcatgtac 
aaagtatct cagataata aatttaaat ttaagaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY