Status
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Gene information
General information
TC014465 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10680279..10684566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014465
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02288

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 5
    vitellogenic egg chamber late completenot present - Number of animals showing the phenotype: 5

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection

    Pupal morphology
    eyes decreased - Number of animals showing the phenotype: 4

    ommatidia irregular - Number of animals showing the phenotype: 4
    Adult morphology
    eyes decreased - Number of animals showing the phenotype: 4


    ommatidia irregular - Number of animals showing the phenotype: 4

    elytra distalpigmentation/tanning decreased - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014465 RA
      MADTGYKNR IQNFKNKGK DQDEMRRRR NEVTVELRK 
NKREETLQK RRNVPVVDS TDEDDIEKS LSTTNLEEL 
VAQAAAIDN PPLQLMAIQ TARKLLSSD RNPPIDALI 
SSGILPVLV MCLERYDEP SLQFEAAWA LTNIASGTS 
AQTNRVVQA GAVPLFLKL LHSPQQNVC EQAVWALGN 
IIGDGPQLR DYVIELGVV NPLLSFIKP DVPISFLRN 
VTWVIVNLC RNKDPPPPI QTIRELLPA LNALIHHTD 
TNILVDTVW ALSYLTDGG NEQIQMVID SGVVPKLIP 
LLSHKEVKV QTAALRAVG NIVTGTDEQ TQVVLNCDA 
LSHFPALLS HSKEKICKE AVWFLSNIT AGNQMQVQA 
VIDAGLLPN IINNLSKGD FQTQKEAAW AISNLTIGG 
NKEQVATLI REGVIPPFC DLLSCKDAQ VIQVVLDGI 
NNMLKMAGA EVDQLCTMI EECNGLDKI EMLQNHDNI 
EIYKIAYDI IEQYFSGDA DDDANLAPT AAETGFQFD 
PNTNVPNEG FKF
    CDS sequence
    • >TC014465 RA
      atggcggat acaggttac aagaatcgc atccagaat 
ttcaagaac aagggaaaa gaccaagat gaaatgcga 
agacggcgc aacgaggtc accgtggaa ttgcgcaag 
aacaagcgc gaggagacc ctccagaag cgccgcaat 
gtccccgtg gtggactcc accgacgag gacgacatc 
gagaagtcg ctctccacc acgaacttg gaagaactg 
gtggcgcaa gcggccgct atcgacaac cccccgctg 
cagctgatg gcgatccaa accgcgcgg aagttgctg 
tcttcagac aggaacccc ccgatcgac gccctcatc 
tccagcggg atcctgccg gtcctggtg atgtgtctc 
gaacggtat gacgagccc tcgttgcag tttgaggcc 
gcctgggcg ctcacgaac atcgcctcg ggcacgtcc 
gcgcagacc aacagggtg gtgcaagcg ggggccgtg 
cccctcttc ctgaagctg ctgcactcg ccccagcag 
aacgtctgt gaacaggcc gtttgggcc ctggggaat 
atcatcggc gatgggccg cagttaaga gactatgtt 
atcgagctc ggtgttgtt aatccactt ctctcgttc 
attaaaccc gacgttccg atttcgttc ttgaggaac 
gtcacctgg gttattgtt aatttgtgt aggaacaag 
gatcccccg ccgcctata cagactatt agggagttg 
ttacccgcc ttgaatgcg cttattcac cacacagat 
accaatatt ttagtcgac acggtctgg gcattaagt 
tacctcacc gacggcggc aacgaacaa attcaaatg 
gtgatcgac agcggcgtc gtgcccaaa ctcatccct 
ctcctcagc cacaaggag gtgaaagtg cagacggcg 
gccctccgt gccgtcgga aacatcgtc accggaacg 
gacgagcaa acccaggtg gtcctcaac tgcgacgcc 
ttgtcccac ttccctgcc ctcctctcc cactccaaa 
gagaagatc tgtaaagag gccgtctgg ttcctgtct 
aacatcacc gccggtaac cagatgcaa gtccaggcc 
gtgattgac gccggcctc ctgcccaac atcatcaac 
aacctcagc aagggcgat tttcaaacg cagaaagag 
gcggcgtgg gccatcagt aatctaacc attggcgga 
aacaaggaa caggtggcc acactgatc cgcgaaggg 
gtgattccc ccgttctgc gacctcctc agttgtaaa 
gatgcgcaa gtgattcag gtcgttttg gacgggatt 
aacaacatg ctgaagatg gcgggagcg gaggtggac 
cagttgtgt accatgatt gaggagtgc aatggctta 
gataaaatc gaaatgttg caaaatcat gacaatata 
gaaatttat aaaattgca tatgacatc atcgaacag 
tactttagt ggtgatgcg gatgatgat gcaaactta 
gcaccgacc gccgctgaa accggattc caattcgat 
ccaaatacc aacgtaccg aacgaggga ttcaaattc 
tga
    mRNA sequence
    • >TC014465 RA
      gccaacttt aaaaactga aagttttgc cgaaattat 
tgcaaataa aggtgtagt gtccattga aacaataaa 
tttggcttg attttgagc ctacagtga aaagttgtg 
gttttaatg ttaataagg tttagattg tgttaattt 
tcccgatca gctgtcctt ccgttgtgt ttgccaccg 
tgcgcgcaa cctgtgcaa ccgatcttt gtagcaaat 
gggtatgaa aaaaatacc caaattacg ttaaatcag 
tcggtaatt ttgtgataa tcgcgtccg aacgtttcc 
cccttcatt tttcatgtt atcataacg ccaaatcga 
gtcttttac gttttaaga gcgccaccc ggcttttcc 
tcgtgtttt catcagccc tccattgtc aaatctatc 
aaaatggcg gatacaggt tacaagaat cgcatccag 
aatttcaag aacaaggga aaagaccaa gatgaaatg 
cgaagacgg cgcaacgag gtcaccgtg gaattgcgc 
aagaacaag cgcgaggag accctccag aagcgccgc 
aatgtcccc gtggtggac tccaccgac gaggacgac 
atcgagaag tcgctctcc accacgaac ttggaagaa 
ctggtggcg caagcggcc gctatcgac aaccccccg 
ctgcagctg atggcgatc caaaccgcg cggaagttg 
ctgtcttca gacaggaac cccccgatc gacgccctc 
atctccagc gggatcctg ccggtcctg gtgatgtgt 
ctcgaacgg tatgacgag ccctcgttg cagtttgag 
gccgcctgg gcgctcacg aacatcgcc tcgggcacg 
tccgcgcag accaacagg gtggtgcaa gcgggggcc 
gtgcccctc ttcctgaag ctgctgcac tcgccccag 
cagaacgtc tgtgaacag gccgtttgg gccctgggg 
aatatcatc ggcgatggg ccgcagtta agagactat 
gttatcgag ctcggtgtt gttaatcca cttctctcg 
ttcattaaa cccgacgtt ccgatttcg ttcttgagg 
aacgtcacc tgggttatt gttaatttg tgtaggaac 
aaggatccc ccgccgcct atacagact attagggag 
ttgttaccc gccttgaat gcgcttatt caccacaca 
gataccaat attttagtc gacacggtc tgggcatta 
agttacctc accgacggc ggcaacgaa caaattcaa 
atggtgatc gacagcggc gtcgtgccc aaactcatc 
cctctcctc agccacaag gaggtgaaa gtgcagacg 
gcggccctc cgtgccgtc ggaaacatc gtcaccgga 
acggacgag caaacccag gtggtcctc aactgcgac 
gccttgtcc cacttccct gccctcctc tcccactcc 
aaagagaag atctgtaaa gaggccgtc tggttcctg 
tctaacatc accgccggt aaccagatg caagtccag 
gccgtgatt gacgccggc ctcctgccc aacatcatc 
aacaacctc agcaagggc gattttcaa acgcagaaa 
gaggcggcg tgggccatc agtaatcta accattggc 
ggaaacaag gaacaggtg gccacactg atccgcgaa 
ggggtgatt cccccgttc tgcgacctc ctcagttgt 
aaagatgcg caagtgatt caggtcgtt ttggacggg 
attaacaac atgctgaag atggcggga gcggaggtg 
gaccagttg tgtaccatg attgaggag tgcaatggc 
ttagataaa atcgaaatg ttgcaaaat catgacaat 
atagaaatt tataaaatt gcatatgac atcatcgaa 
cagtacttt agtggtgat gcggatgat gatgcaaac 
ttagcaccg accgccgct gaaaccgga ttccaattc 
gatccaaat accaacgta ccgaacgag ggattcaaa 
ttctgattc atttctata ttgctcaga gtaactatt 
ttgatctag cgcgtttgc ttcctttcc tccctaaat 
tcttttacg gtggtttat gtacgctca ttttatacg 
gttgtgtaa atatagaat tttttataa ataattatg 
ctcgtaatt gctttagga tatttattt aatttaaac 
ggaaaaata tgtcactgt attttagtt tttatttta 
ttttgtaca tttaaatta ggtaaatat caacattaa 
tgtaaaatt tttaaaatg accgtacat aagagacaa 
tctgggcat gatctaata aatcaaaaa acagtaagt 
cattaacgg gattttaac aattggaca tgtcatgcg 
cagatttgt atattttta tgatattaa cagtttttt 
ggtaaacat catgctata tacctgcgt gttttttaa 
gtttacaac gtttttttt tcgttgttt gtagtcata 
tacataatg tttatcgaa aaagaacta aataaattg 
agaatttta attgactgg tcgcgttta attaatatg 
attaaggct attatgtaa ttaaaaagt atagtttcg 
tgcttacca ttgtattat ggtagttgg catcgttta 
aatataatt taaaataaa caaagtcga atctt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY