Status
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Gene information
General information
TC014309 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8924611..8926814
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014309
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05607

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014309 RA
      MEATFVVVG GGIAGVSCA ETLAFLEPE KTIILISES 
      SLIKTVTNL HAITRTLTD FDINETDSS YLTNKYLNI 
      TVVHDKLTE INDTDHFIV TSSGQIKYE FLCLCMGAE 
      PKLIPQASS FSQIIGIRD TDSVETFVQ KLKTAKTIV 
      VVGNGGIAS EVVFKIRNV DVHWVVRDK HISATFVDP 
      GAAEFFQSA LNHGKSGEE PVYKRMRYS EDSFTKSGA 
      ALGPDWYNN LSITGGKGA SSTDVKIHY EAEVTNVEA 
      LENCDFPIK VALTSGLKI PCDLVVSAT GVLPRRNFA 
      TLHEFARSE DGGLQVNEF MQTSLKDIY AAGDICAAG 
      WPPAKHWFQ MKLWTQARQ MGCYAAKCM SAHSNNESL 
      LQDFCFEMF AHSTKLFGY KVILLGLYN GQKLGRNYE 
      ILLRMTKEK EYIKFVLED NKLQGAVLI GDTDLEEMC 
      ENLILNQLD LAPYGDDIL NPNIDIEDY FD
    CDS sequence
    • >TC014309 RA
      atggaggcg acgtttgtg gtagttggg ggaggaata 
      gctggagtt agttgtgca gaaactttg gcgtttctg 
      gagcctgag aaaacaata attttgatt agtgaatcc 
      tcccttatt aagacagtt acaaattta cacgcaatt 
      acgcgaact ttgaccgat tttgatatt aatgaaacg 
      gattcaagc tatttaaca aacaaatat ttgaatatt 
      actgtggtc cacgataaa ctcaccgaa atcaacgac 
      actgaccac ttcatagtc acgtctagt gggcaaatt 
      aaatacgaa tttttgtgt ttgtgtatg ggggctgag 
      ccaaaacta atcccccaa gcctcgtct ttttctcaa 
      atcattggt attagagac accgattcg gtcgaaact 
      ttcgtacaa aaattgaaa acagccaag acaattgtc 
      gttgtcggt aacgggggc atcgcctca gaggtggtt 
      ttcaaaatc agaaacgta gacgtccac tgggtggtg 
      cgtgacaag cacatttcc gcgactttc gtcgaccct 
      ggggctgct gaattcttc caatccgcc ttaaaccac 
      ggaaaatcg ggcgaggag ccggtttac aaaagaatg 
      agatacagt gaggacagc ttcaccaaa tcgggggcc 
      gctttgggg cctgactgg tacaacaac ctgtccatc 
      actgggggc aaaggcgcc tctagtacc gacgtgaaa 
      atccactac gaagccgag gtcacaaac gtcgaagct 
      ttggaaaat tgcgatttc ccgattaag gtcgctcta 
      accagcggt ttgaaaatc ccctgcgac cttgtcgtt 
      tccgccacc ggggtgctc ccaagacgc aactttgcg 
      actttgcac gagttcgca aggtccgaa gatgggggg 
      ctccaagtc aacgagttc atgcaaact tcgcttaaa 
      gacatctac gccgcgggg gatatttgt gccgccggt 
      tggccccca gctaagcac tggttccag atgaagttg 
      tggacgcag gcgagacag atggggtgt tacgcggcc 
      aagtgcatg tcagctcat tcgaacaac gagagcttg 
      ctgcaggat ttttgcttc gaaatgttc gcacattcg 
      accaaactt ttcgggtat aaagtcatc ctgctgggg 
      ctttataac ggccagaag ttggggcgt aattacgaa 
      attttgtta aggatgacc aaggagaag gaatacatc 
      aagtttgtt ctcgaagat aataagtta caaggggct 
      gttctcatc ggcgataca gatttggag gaaatgtgc 
      gagaactta attttgaat cagttggat ctggcgcct 
      tacggcgac gatattttg aacccaaac atcgatatt 
      gaggattat tttgattga
    mRNA sequence
    • >TC014309 RA
      ggatgcggc ttgaaaatt aatcaataa aaaccaata 
      aaaaaacac agtttcaaa tttgactcc cccagtccc 
      cacattgac attcgaatc cgacactaa cctaaaata 
      tggcaatgt tatcctcaa taattgtga agaatttac 
      ccgagaacc ccttatgga aacgcactt ttaccatat 
      cggcagcat tcagagcat cagaaacgg ccttatctg 
      ctgagccaa ggctcgaag ttcgcgaaa taaccaaag 
      accgctcga aacccatcc ccgttgaga caagtataa 
      ggtacctaa agagcgccg cttaccggc aaacgtacg 
      gcgaccagc ctgtgtggg tcctgtacc gacgaaacc 
      acaaagggg ctatccccc ccaggaaaa cacggaaaa 
      cctgcattc ggggggacc aaatttcgt gcgggaacc 
      cctgtccca tctgccggg acgagtact tggttttac 
      acgaaaata acgtagaat tgcttaaac agttcgtct 
      gtgaacaca ctggtagta ttttgagtt attccaaaa 
      ctgggttgt gccaaaaga agcaccaac agttgctag 
      tggcgattc gaagggctg tggacagcg gactgctca 
      cctttgatg tgccgcacc gacactaca attattccg 
      aattttacc ccgaactga agcaataac atggaggcg 
      acgtttgtg gtagttggg ggaggaata gctggagtt 
      agttgtgca gaaactttg gcgtttctg gagcctgag 
      aaaacaata attttgatt agtgaatcc tcccttatt 
      aagacagtt acaaattta cacgcaatt acgcgaact 
      ttgaccgat tttgatatt aatgaaacg gattcaagc 
      tatttaaca aacaaatat ttgaatatt actgtggtc 
      cacgataaa ctcaccgaa atcaacgac actgaccac 
      ttcatagtc acgtctagt gggcaaatt aaatacgaa 
      tttttgtgt ttgtgtatg ggggctgag ccaaaacta 
      atcccccaa gcctcgtct ttttctcaa atcattggt 
      attagagac accgattcg gtcgaaact ttcgtacaa 
      aaattgaaa acagccaag acaattgtc gttgtcggt 
      aacgggggc atcgcctca gaggtggtt ttcaaaatc 
      agaaacgta gacgtccac tgggtggtg cgtgacaag 
      cacatttcc gcgactttc gtcgaccct ggggctgct 
      gaattcttc caatccgcc ttaaaccac ggaaaatcg 
      ggcgaggag ccggtttac aaaagaatg agatacagt 
      gaggacagc ttcaccaaa tcgggggcc gctttgggg 
      cctgactgg tacaacaac ctgtccatc actgggggc 
      aaaggcgcc tctagtacc gacgtgaaa atccactac 
      gaagccgag gtcacaaac gtcgaagct ttggaaaat 
      tgcgatttc ccgattaag gtcgctcta accagcggt 
      ttgaaaatc ccctgcgac cttgtcgtt tccgccacc 
      ggggtgctc ccaagacgc aactttgcg actttgcac 
      gagttcgca aggtccgaa gatgggggg ctccaagtc 
      aacgagttc atgcaaact tcgcttaaa gacatctac 
      gccgcgggg gatatttgt gccgccggt tggccccca 
      gctaagcac tggttccag atgaagttg tggacgcag 
      gcgagacag atggggtgt tacgcggcc aagtgcatg 
      tcagctcat tcgaacaac gagagcttg ctgcaggat 
      ttttgcttc gaaatgttc gcacattcg accaaactt 
      ttcgggtat aaagtcatc ctgctgggg ctttataac 
      ggccagaag ttggggcgt aattacgaa attttgtta 
      aggatgacc aaggagaag gaatacatc aagtttgtt 
      ctcgaagat aataagtta caaggggct gttctcatc 
      ggcgataca gatttggag gaaatgtgc gagaactta 
      attttgaat cagttggat ctggcgcct tacggcgac 
      gatattttg aacccaaac atcgatatt gaggattat 
      tttgattga atgtgtaaa tttcgtaat tgttaataa 
      atttttgag ttactt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY