Status
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Gene information
General information
TC013713 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5463690..5466438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013713
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013713 RA
      MNRRDYDKD KETIRTFLL EFCIQDDSG QKNFKYSNQ 
LTKLAHREQ IALYIELDD LYEHDDDLA KAVINNTRR 
YTTLFSDVV FELLPTFVE REVVAKDAL DVYIEHRLM 
MEQRLRQPN EQRDARNKF PPELLRRYE IYFKDLSTT 
KTVPIREVK AEHIGKLVT VRGIVTRST EVKPMMTVA 
TYTCDQCGA ETYQPVHGL SFMPVLMCP SEDCRVNKS 
GGRLYLQTR GSKFVKFQE LKIQEHSDQ VPVGHIPRT 
LTIFCRGEV TRLALPGHH VAVTGVFLP LLKTGFKQI 
MGGLLSETY VEAHRIALV NKTEEDESS SKPLTPEEL 
SVLTEDDFY TKLAVSLAP EIYGHLDVK KALLLLLVG 
GVDRRPDGM KIRGNINIC LMGDPGVAK SQLLGYIDR 
LAPRSQYTT GRGSSGVGL TASVMKDPM TGEMMLEGG 
ALVLADQGV CCIDEFDKM ADADRTAIH EVMEQQTIS 
IAKAGIMTC LNARVSILA AANPAYGRY NPKRTIEQN 
IQLPAALLS RFDLLWLIQ DKADRDNDL KLAKHITYV 
HQHCKQPPT QIKSLDMGV MRKYIALCK LKEPVIPED 
LTEYIVNAY VELRNVARN SRDMTFTSA RNLLGILRL 
STALARLRL SNVVEKDDV NEAIRLMEM SKDSLNQTF 
EKGARPPNV NDQIFAIIR DLAGESKTV KLSDVMNKC 
ASKGHTPLE VDSCIEEYE ELNVWQVNL TRTKITFI
    CDS sequence
    • >TC013713 RA
      atgaaccga cgtgattac gacaaagat aaagagacc 
ataagaacc ttcttgtta gagttttgc attcaagat 
gattccggt cagaaaaac tttaaatat tcgaatcag 
ttgacaaaa ctggctcat cgcgagcaa atagccctt 
tacatcgag cttgacgat ctttacgaa cacgacgat 
gatttagcc aaagctgta ataaacaac actcgtcgg 
tacacgact ttattctcc gacgttgtc ttcgaactg 
ttgcccaca ttcgtggaa cgcgaagtc gtggccaaa 
gacgctttg gacgtttac attgaacac aggctaatg 
atggagcag cgtttgaga caaccgaac gagcagaga 
gatgctcgt aataaattt ccaccggaa ttattacgc 
agatacgaa atctacttc aaagactta tcaactact 
aaaaccgtc ccaattcgc gaagtcaaa gccgaacac 
attgggaaa ctggtcacc gttaggggc atagtgacg 
cgaagcacc gaagtcaaa cctatgatg accgtagcg 
acctacact tgcgaccaa tgcggggct gagacctac 
caacctgtc cacggacta agtttcatg ccggtgttg 
atgtgtcct agtgaagac tgccgtgtg aacaaatca 
ggggggcgc ctatacctc caaacccgt ggctccaaa 
ttcgtcaaa ttccaggag ctcaaaatc caggaacac 
agtgaccaa gtcccagtg gggcacatc ccccggact 
ttgactatt ttttgtcgg ggcgaagtc actcgcctg 
gcccttccg gggcaccac gtggccgtc acaggcgtg 
ttcctccct ttgctcaag accggcttc aaacaaatc 
atggggggg ctcctgtcg gagacttac gtcgaggcg 
cacagaatc gctttggtg aacaaaacc gaagaggat 
gaaagctcg tccaaaccc ctcacacct gaagaacta 
tcggtcttg accgaagac gatttttac acaaaattg 
gcggtgtct ctagcgccg gaaatctac ggtcatctt 
gacgttaag aaggctttg cttcttttg ctggttggg 
ggagtggac cgcagacct gacggtatg aagatccgg 
gggaatatc aatatttgc ctgatgggg gaccccgga 
gtcgccaag tcgcaactt ttggggtac attgatcgg 
ttagcgcca cgaagtcag tataccaca ggacggggc 
tcttcggga gtggggctc actgcctcg gttatgaag 
gaccccatg acgggggag atgatgctg gaagggggc 
gcactggtc ttggccgat cagggggtt tgttgtatt 
gacgaattc gataaaatg gcggatgcg gacaggacg 
gccattcac gaagttatg gaacaacaa acaatcagc 
attgctaag gcgggtatt atgacttgt ttgaacgcc 
agagtctcg attttggcg gcggctaac ccggcctat 
ggccggtac aacccgaag cggacaatc gagcaaaat 
atccaacta ccggcggcc cttttatcc cgtttcgat 
ctcctttgg ttgattcag gataaagcg gatcgcgat 
aatgatctg aaactcgcc aaacacatc acatacgtg 
caccagcac tgcaaacag cccccgact caaatcaaa 
tcgcttgat atgggcgtt atgcggaaa tacatcgca 
ctgtgtaag ttgaaagaa ccggtgatt cccgaagat 
ttgactgaa tacatcgtt aatgcttat gtggagttg 
agaaatgtg gcccggaat tctagagac atgactttt 
acttcggct aggaatttg ttggggatt ttgaggctg 
tcgacggct ttagccagg ctgagattg tccaatgtt 
gtggagaaa gacgatgtt aatgaagct ataaggttg 
atggaaatg agcaaagat tctcttaac cagactttc 
gaaaaaggg gcaagacca cccaatgtc aacgaccaa 
atctttgcc attattaga gacttggct ggagaatcg 
aaaacagtc aaactatca gacgttatg aacaaatgt 
gccagtaag ggacacact cctctggaa gttgattcc 
tgtatcgaa gagtatgag gaattgaac gtgtggcaa 
gtgaatctg actcgcacc aaaatcaca tttatttaa
    mRNA sequence
    • >TC013713 RA
      aaactggcg tgtgttgtg tgcaaacag gtggtcgta 
aataaacgt tagtaaaac tccaattcc ggcttgaag 
tagtgtaaa ttcgccaaa aatcatgaa ccgacgtga 
ttacgacaa agataaaga gaccataag aaccttctt 
gttagagtt ttgcattca agatgattc cggtcagaa 
aaactttaa atattcgaa tcagttgac aaaactggc 
tcatcgcga gcaaatagc cctttacat cgagcttga 
cgatcttta cgaacacga cgatgattt agccaaagc 
tgtaataaa caacactcg tcggtacac gactttatt 
ctccgacgt tgtcttcga actgttgcc cacattcgt 
ggaacgcga agtcgtggc caaagacgc tttggacgt 
ttacattga acacaggct aatgatgga gcagcgttt 
gagacaacc gaacgagca gagagatgc tcgtaataa 
atttccacc ggaattatt acgcagata cgaaatcta 
cttcaaaga cttatcaac tactaaaac cgtcccaat 
tcgcgaagt caaagccga acacattgg gaaactggt 
caccgttag gggcatagt gacgcgaag caccgaagt 
caaacctat gatgaccgt agcgaccta cacttgcga 
ccaatgcgg ggctgagac ctaccaacc tgtccacgg 
actaagttt catgccggt gttgatgtg tcctagtga 
agactgccg tgtgaacaa atcaggggg gcgcctata 
cctccaaac ccgtggctc caaattcgt caaattcca 
ggagctcaa aatccagga acacagtga ccaagtccc 
agtggggca catcccccg gactttgac tattttttg 
tcggggcga agtcactcg cctggccct tccggggca 
ccacgtggc cgtcacagg cgtgttcct ccctttgct 
caagaccgg cttcaaaca aatcatggg ggggctcct 
gtcggagac ttacgtcga ggcgcacag aatcgcttt 
ggtgaacaa aaccgaaga ggatgaaag ctcgtccaa 
acccctcac acctgaaga actatcggt cttgaccga 
agacgattt ttacacaaa attggcggt gtctctagc 
gccggaaat ctacggtca tcttgacgt taagaaggc 
tttgcttct tttgctggt tgggggagt ggaccgcag 
acctgacgg tatgaagat ccgggggaa tatcaatat 
ttgcctgat gggggaccc cggagtcgc caagtcgca 
acttttggg gtacattga tcggttagc gccacgaag 
tcagtatac cacaggacg gggctcttc gggagtggg 
gctcactgc ctcggttat gaaggaccc catgacggg 
ggagatgat gctggaagg gggcgcact ggtcttggc 
cgatcaggg ggtttgttg tattgacga attcgataa 
aatggcgga tgcggacag gacggccat tcacgaagt 
tatggaaca acaaacaat cagcattgc taaggcggg 
tattatgac ttgtttgaa cgccagagt ctcgatttt 
ggcggcggc taacccggc ctatggccg gtacaaccc 
gaagcggac aatcgagca aaatatcca actaccggc 
ggccctttt atcccgttt cgatctcct ttggttgat 
tcaggataa agcggatcg cgataatga tctgaaact 
cgccaaaca catcacata cgtgcacca gcactgcaa 
acagccccc gactcaaat caaatcgct tgatatggg 
cgttatgcg gaaatacat cgcactgtg taagttgaa 
agaaccggt gattcccga agatttgac tgaatacat 
cgttaatgc ttatgtgga gttgagaaa tgtggcccg 
gaattctag agacatgac ttttacttc ggctaggaa 
tttgttggg gattttgag gctgtcgac ggctttagc 
caggctgag attgtccaa tgttgtgga gaaagacga 
tgttaatga agctataag gttgatgga aatgagcaa 
agattctct taaccagac tttcgaaaa aggggcaag 
accacccaa tgtcaacga ccaaatctt tgccattat 
tagagactt ggctggaga atcgaaaac agtcaaact 
atcagacgt tatgaacaa atgtgccag taagggaca 
cactcctct ggaagttga ttcctgtat cgaagagta 
tgaggaatt gaacgtgtg gcaagtgaa tctgactcg 
caccaaaat cacatttat ttaagattt agttgtttg 
taccacttt tataacttg tatttttta tatttcaat 
aaagcatga atccaatt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY