Status
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Gene information
General information
TC013546 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8216413..8219103
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013546
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_02155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013546 RA
      MCDVNESAS TSFISLSSS EESLNDENC DICDNPETI 
      RVYLRIKNG YDLKDLYEI EGNTLVSKV PPGSNFLRN 
      IKEGDSLKK IHAFTKIFE PQTTQRDIF NDVVKPKIF 
      DFINGQNST LLTYGASGS GKTFTVIGT DEEPGIVPR 
      SLEYLFRTL PQLMSDPAA KPSPAGNVT LLSDSDCKK 
      EKLLCRNLL NSSSAVGDR MQHVRIYKA MQQRLSSEP 
      VAFLEDCND LSIGVWISF AEIYNEQIY DLLRANPSK 
      KEQRPRLRL GMSKGQVYI KNLTCVNVR SGEEAYQIL 
      QYGLNNLNY APTRVNEHS SRSHSIFTI KLVQVSKSD 
      GGCFVSSFN FCDLAGSER SKKTLNVGD RLRESNNIN 
      TSLLVLGRC ISAVRNAQK AHDNKLVPF RESKLTQLF 
      QKALSGGED IAMIVTINP SREMFDESQ HVLNFSAVA 
      SEISIETAT KQKETVRDV LKNKTCTEE DFEYTSEEL 
      QNMILLLRD ELEIQRNEY ETNLKIERE YLRKGYEEI 
      YQDHENYVQ ERIKFAEER VRRQYEDEI EAYKDKIKE 
      LENRSEVIE LSSDEESEA KETRRRVTN LRRTLDEGF 
      GRYRAAEEK CRQLEIENI QLKERIFEL ENELEAFRQ 
      HHASVISEE IEGSETYGE NGVAFNLWD D
    • >TC013546 RB
      MFEYDSLVM KNASFLRAR DPSILTYNR SLIPGARTN 
      LDKDFAEET YQQKNQPET IRVYLRIKN GYDLKDLYE 
      IEGNTLVSK VPPGSNFLR NIKEGDSLK KIHAFTKIF 
      EPQTTQRDI FNDVVKPKI FDFINGQNS TLLTYGASG 
      SGKTFTVIG TDEEPGIVP RSLEYLFRT LPQLMSDPA 
      AKPSPAGNV TLLSDSDCK KEKLLCRNL LNSSSAVGD 
      RMQHVRIYK AMQQRLSSE PVAFLEDCN DLSIGVWIS 
      FAEIYNEQI YDLLRANPS KKEQRPRLR LGMSKGQVY 
      IKNLTCVNV RSGEEAYQI LQYGLNNLN YAPTRVNEH 
      SSRSHSIFT IKLVQVSKS DGGCFVSSF NFCDLAGSE 
      RSKKTLNVG DRLRESNNI NTSLLVLGR CISAVRNAQ 
      KAHDNKLVP FRESKLTQL FQKALSGGE DIAMIVTIN 
      PSREMFDES QHVLNFSAV ASEISIETA TKQKETVRD 
      VLKNKTCTE EDFEYTSEE LQNMILLLR DELEIQRNE 
      YETNLKIER EYLRKGYEE IYQDHENYV QERIKFAEE 
      RVRRQYEDE IEAYKDKIK ELENRSEVI ELSSDEESE 
      AKETRRRVT NLRRTLDEG FGRYRAAEE KCRQLEIEN 
      IQLKERIFE LENELEAFR QHHASVISE EIEGSETYG 
      ENGVAFNLW DD
    • >TC013546 RC
      MCDVNESAS TSFISLSSS EESLNDENC DICDNVEFF 
      YTPHFSNFR LTFQPETIR VYLRIKNGY DLKDLYEIE 
      GNTLVSKVP PGSNFLRNI KEGDSLKKI HAFTKIFEP 
      QTTQRDIFN DVVKPKIFD FINGQNSTL LTYGASGSG 
      KTFTVIGTD EEPGIVPRS LEYLFRTLP QLMSDPAAK 
      PSPAGNVTL LSDSDCKKE KLLCRNLLN SSSAVGDRM 
      QHVRIYKAM QQRLSSEPV AFLEDCNDL SIGVWISFA 
      EIYNEQIYD LLRANPSKK EQRPRLRLG MSKGQVYIK 
      NLTCVNVRS GEEAYQILQ YGLNNLNYA PTRVNEHSS 
      RSHSIFTIK LVQVSKSDG GCFVSSFNF CDLAGSERS 
      KKTLNVGDR LRESNNINT SLLVLGRCI SAVRNAQKA 
      HDNKLVPFR ESKLTQLFQ KALSGGEDI AMIVTINPS 
      REMFDESQH VLNFSAVAS EISIETATK QKETVRDVL 
      KNKTCTEED FEYTSEELQ NMILLLRDE LEIQRNEYE 
      TNLKIEREY LRKGYEEIY QDHENYVQE RIKFAEERV 
      RRQYEDEIE AYKDKIKEL ENRSEVIEL SSDEESEAK 
      ETRRRVTNL RRTLDEGFG RYRAAEEKC RQLEIENIQ 
      LKERIFELE NELEAFRQH HASVISEEI EGSETYGEN 
      GVAFNLWDD
    CDS sequence
    • >TC013546 RA
      atgtgtgac gttaacgaa tctgcttcc acttctttc 
      atcagttta agttcaagt gaagaaagc ttgaatgac 
      gaaaattgt gatatttgc gataatccc gaaaccatc 
      cgtgtttat ctccgcata aaaaacggc tatgacttg 
      aaagacttg tacgaaatt gagggaaat accctcgtt 
      tcgaaggtg cccccaggt tcgaatttc ctgcgcaac 
      atcaaagaa ggtgactct ttgaagaaa atccacgct 
      tttacaaaa atcttcgag cctcaaaca acccaaaga 
      gatattttc aatgacgtc gttaaacca aaaattttt 
      gatttcata aatggacag aattccact cttttaact 
      tatggcgct tcaggatct ggaaaaaca ttcactgtc 
      attgggact gatgaagaa ccaggaatc gttcctcgt 
      tctttggaa tatctcttc cgtactttg cctcaactc 
      atgtcagat cctgctgcc aagccatca cctgcagga 
      aatgtgaca ctattgagt gattccgac tgcaagaaa 
      gaaaaattg ctgtgtaga aatctcctg aattcgtct 
      tcagcggtg ggggatcga atgcagcac gtcaggatt 
      tacaaggca atgcaacaa agactgagc agcgaaccg 
      gtcgctttt ctggaagac tgcaatgac ttatctatc 
      ggcgtgtgg atctctttt gccgaaata tacaacgag 
      caaatttac gacctgctt cgcgcaaac ccttcgaaa 
      aaagaacaa cgaccaaga ctgcgctta ggcatgtct 
      aaaggtcaa gtctacatt aaaaacctg acatgcgtg 
      aatgtaaga agtggagag gaagcatat cagatcctc 
      cagtacggg ctaaacaat ctcaattat gcaccaacc 
      cgagtaaac gaacactca agtcgctcc cattcgatt 
      tttacgata aaattggta caagtttca aagtcagat 
      ggtggctgc ttcgttagc agctttaat ttttgtgat 
      ttggctggt tccgaacgc agtaaaaag accctaaat 
      gtgggtgat agactccga gaatccaac aatattaac 
      acctctttg ttggtcctg gggcggtgc atttctgca 
      gtgagaaac gcacaaaag gcgcatgat aataaattg 
      gttccgttt agagagtca aaattgacc caattgttc 
      caaaaggcg ttatcagga ggcgaagac attgcgatg 
      attgttact ataaatccc tcacgcgaa atgttcgac 
      gaatctcaa cacgtgctc aacttttcc gccgttgca 
      agcgaaatc agcatcgaa actgccacc aaacagaaa 
      gaaacagtt cgagatgtt ttaaaaaat aaaacatgt 
      acggaggaa gactttgag tacacaagt gaagaactg 
      caaaacatg attttgctt ctacgagat gaactcgaa 
      atccaacga aacgagtac gaaaccaac ttgaaaatc 
      gaaagagag tatttaagg aagggctac gaggaaatc 
      taccaagac cacgagaat tatgtgcaa gaaaggatt 
      aaatttgca gaggagcgg gtgagaagg caatacgaa 
      gacgaaatc gaggcttat aaagataaa atcaaagag 
      ttggaaaat agatctgaa gttattgaa ctgagctcg 
      gatgaagaa tcggaagct aaggaaact agaaggaga 
      gttactaac cttaggagg actttagat gaagggttt 
      ggtcgatat cgggcagct gaggaaaaa tgcagacaa 
      ctcgaaatt gaaaatatt caattaaag gagcgaatt 
      tttgaattg gaaaatgag ctagaggcg ttcagacaa 
      catcacgcg tcggtgatc agtgaagaa attgaaggt 
      tccgaaacg tatggtgaa aatggtgtg gcttttaac 
      ttatgggat gattaa
    • >TC013546 RB
      atgtttgaa tacgacagt ttggtaatg aaaaatgct 
      tcgttcttg agagctcga gatcccagt attttaaca 
      tacaatcgg tccttaata cctggcgca aggacaaat 
      ctagataag gactttgct gaagaaact tatcaacaa 
      aaaaatcag cccgaaacc atccgtgtt tatctccgc 
      ataaaaaac ggctatgac ttgaaagac ttgtacgaa 
      attgaggga aataccctc gtttcgaag gtgccccca 
      ggttcgaat ttcctgcgc aacatcaaa gaaggtgac 
      tctttgaag aaaatccac gcttttaca aaaatcttc 
      gagcctcaa acaacccaa agagatatt ttcaatgac 
      gtcgttaaa ccaaaaatt tttgatttc ataaatgga 
      cagaattcc actctttta acttatggc gcttcagga 
      tctggaaaa acattcact gtcattggg actgatgaa 
      gaaccagga atcgttcct cgttctttg gaatatctc 
      ttccgtact ttgcctcaa ctcatgtca gatcctgct 
      gccaagcca tcacctgca ggaaatgtg acactattg 
      agtgattcc gactgcaag aaagaaaaa ttgctgtgt 
      agaaatctc ctgaattcg tcttcagcg gtgggggat 
      cgaatgcag cacgtcagg atttacaag gcaatgcaa 
      caaagactg agcagcgaa ccggtcgct tttctggaa 
      gactgcaat gacttatct atcggcgtg tggatctct 
      tttgccgaa atatacaac gagcaaatt tacgacctg 
      cttcgcgca aacccttcg aaaaaagaa caacgacca 
      agactgcgc ttaggcatg tctaaaggt caagtctac 
      attaaaaac ctgacatgc gtgaatgta agaagtgga 
      gaggaagca tatcagatc ctccagtac gggctaaac 
      aatctcaat tatgcacca acccgagta aacgaacac 
      tcaagtcgc tcccattcg atttttacg ataaaattg 
      gtacaagtt tcaaagtca gatggtggc tgcttcgtt 
      agcagcttt aatttttgt gatttggct ggttccgaa 
      cgcagtaaa aagacccta aatgtgggt gatagactc 
      cgagaatcc aacaatatt aacacctct ttgttggtc 
      ctggggcgg tgcatttct gcagtgaga aacgcacaa 
      aaggcgcat gataataaa ttggttccg tttagagag 
      tcaaaattg acccaattg ttccaaaag gcgttatca 
      ggaggcgaa gacattgcg atgattgtt actataaat 
      ccctcacgc gaaatgttc gacgaatct caacacgtg 
      ctcaacttt tccgccgtt gcaagcgaa atcagcatc 
      gaaactgcc accaaacag aaagaaaca gttcgagat 
      gttttaaaa aataaaaca tgtacggag gaagacttt 
      gagtacaca agtgaagaa ctgcaaaac atgattttg 
      cttctacga gatgaactc gaaatccaa cgaaacgag 
      tacgaaacc aacttgaaa atcgaaaga gagtattta 
      aggaagggc tacgaggaa atctaccaa gaccacgag 
      aattatgtg caagaaagg attaaattt gcagaggag 
      cgggtgaga aggcaatac gaagacgaa atcgaggct 
      tataaagat aaaatcaaa gagttggaa aatagatct 
      gaagttatt gaactgagc tcggatgaa gaatcggaa 
      gctaaggaa actagaagg agagttact aaccttagg 
      aggacttta gatgaaggg tttggtcga tatcgggca 
      gctgaggaa aaatgcaga caactcgaa attgaaaat 
      attcaatta aaggagcga atttttgaa ttggaaaat 
      gagctagag gcgttcaga caacatcac gcgtcggtg 
      atcagtgaa gaaattgaa ggttccgaa acgtatggt 
      gaaaatggt gtggctttt aacttatgg gatgattaa
    • >TC013546 RC
      atgtgtgac gttaacgaa tctgcttcc acttctttc 
      atcagttta agttcaagt gaagaaagc ttgaatgac 
      gaaaattgt gatatttgc gataatgtt gagtttttt 
      tacacacca catttcagt aactttcga ttgactttc 
      cagcccgaa accatccgt gtttatctc cgcataaaa 
      aacggctat gacttgaaa gacttgtac gaaattgag 
      ggaaatacc ctcgtttcg aaggtgccc ccaggttcg 
      aatttcctg cgcaacatc aaagaaggt gactctttg 
      aagaaaatc cacgctttt acaaaaatc ttcgagcct 
      caaacaacc caaagagat attttcaat gacgtcgtt 
      aaaccaaaa atttttgat ttcataaat ggacagaat 
      tccactctt ttaacttat ggcgcttca ggatctgga 
      aaaacattc actgtcatt gggactgat gaagaacca 
      ggaatcgtt cctcgttct ttggaatat ctcttccgt 
      actttgcct caactcatg tcagatcct gctgccaag 
      ccatcacct gcaggaaat gtgacacta ttgagtgat 
      tccgactgc aagaaagaa aaattgctg tgtagaaat 
      ctcctgaat tcgtcttca gcggtgggg gatcgaatg 
      cagcacgtc aggatttac aaggcaatg caacaaaga 
      ctgagcagc gaaccggtc gcttttctg gaagactgc 
      aatgactta tctatcggc gtgtggatc tcttttgcc 
      gaaatatac aacgagcaa atttacgac ctgcttcgc 
      gcaaaccct tcgaaaaaa gaacaacga ccaagactg 
      cgcttaggc atgtctaaa ggtcaagtc tacattaaa 
      aacctgaca tgcgtgaat gtaagaagt ggagaggaa 
      gcatatcag atcctccag tacgggcta aacaatctc 
      aattatgca ccaacccga gtaaacgaa cactcaagt 
      cgctcccat tcgattttt acgataaaa ttggtacaa 
      gtttcaaag tcagatggt ggctgcttc gttagcagc 
      tttaatttt tgtgatttg gctggttcc gaacgcagt 
      aaaaagacc ctaaatgtg ggtgataga ctccgagaa 
      tccaacaat attaacacc tctttgttg gtcctgggg 
      cggtgcatt tctgcagtg agaaacgca caaaaggcg 
      catgataat aaattggtt ccgtttaga gagtcaaaa 
      ttgacccaa ttgttccaa aaggcgtta tcaggaggc 
      gaagacatt gcgatgatt gttactata aatccctca 
      cgcgaaatg ttcgacgaa tctcaacac gtgctcaac 
      ttttccgcc gttgcaagc gaaatcagc atcgaaact 
      gccaccaaa cagaaagaa acagttcga gatgtttta 
      aaaaataaa acatgtacg gaggaagac tttgagtac 
      acaagtgaa gaactgcaa aacatgatt ttgcttcta 
      cgagatgaa ctcgaaatc caacgaaac gagtacgaa 
      accaacttg aaaatcgaa agagagtat ttaaggaag 
      ggctacgag gaaatctac caagaccac gagaattat 
      gtgcaagaa aggattaaa tttgcagag gagcgggtg 
      agaaggcaa tacgaagac gaaatcgag gcttataaa 
      gataaaatc aaagagttg gaaaataga tctgaagtt 
      attgaactg agctcggat gaagaatcg gaagctaag 
      gaaactaga aggagagtt actaacctt aggaggact 
      ttagatgaa gggtttggt cgatatcgg gcagctgag 
      gaaaaatgc agacaactc gaaattgaa aatattcaa 
      ttaaaggag cgaattttt gaattggaa aatgagcta 
      gaggcgttc agacaacat cacgcgtcg gtgatcagt 
      gaagaaatt gaaggttcc gaaacgtat ggtgaaaat 
      ggtgtggct tttaactta tgggatgat taa
    mRNA sequence
    • >TC013546 RA
      caaatttga aaagtttta acggtgtgt ttgagtttg 
      agtgtaaaa gtgattgtt ttgtgttgt ctctaccaa 
      agttattaa cttattaag tttttaaac aagtaaaaa 
      tgtttgaat acgacagtt tggtaatga aaaatgctt 
      cgttcttga gagctcgag atcccagta ttttaacat 
      acaatcggt ccttaatac ctggcgcaa ggacaaatc 
      tagataagg actttgctg aagaaactt atcaacaaa 
      aaaatcagg taaaaacac tcccttggc gttgcagtg 
      atgattttc gttcaattt ttgtgaaat cgtgagaga 
      tgtgtgacg ttaacgaat ctgcttcca cttctttca 
      tcagtttaa gttcaagtg aagaaagct tgaatgacg 
      aaaattgtg atatttgcg ataatcccg aaaccatcc 
      gtgtttatc tccgcataa aaaacggct atgacttga 
      aagacttgt acgaaattg agggaaata ccctcgttt 
      cgaaggtgc ccccaggtt cgaatttcc tgcgcaaca 
      tcaaagaag gtgactctt tgaagaaaa tccacgctt 
      ttacaaaaa tcttcgagc ctcaaacaa cccaaagag 
      atattttca atgacgtcg ttaaaccaa aaatttttg 
      atttcataa atggacaga attccactc ttttaactt 
      atggcgctt caggatctg gaaaaacat tcactgtca 
      ttgggactg atgaagaac caggaatcg ttcctcgtt 
      ctttggaat atctcttcc gtactttgc ctcaactca 
      tgtcagatc ctgctgcca agccatcac ctgcaggaa 
      atgtgacac tattgagtg attccgact gcaagaaag 
      aaaaattgc tgtgtagaa atctcctga attcgtctt 
      cagcggtgg gggatcgaa tgcagcacg tcaggattt 
      acaaggcaa tgcaacaaa gactgagca gcgaaccgg 
      tcgcttttc tggaagact gcaatgact tatctatcg 
      gcgtgtgga tctcttttg ccgaaatat acaacgagc 
      aaatttacg acctgcttc gcgcaaacc cttcgaaaa 
      aagaacaac gaccaagac tgcgcttag gcatgtcta 
      aaggtcaag tctacatta aaaacctga catgcgtga 
      atgtaagaa gtggagagg aagcatatc agatcctcc 
      agtacgggc taaacaatc tcaattatg caccaaccc 
      gagtaaacg aacactcaa gtcgctccc attcgattt 
      ttacgataa aattggtac aagtttcaa agtcagatg 
      gtggctgct tcgttagca gctttaatt tttgtgatt 
      tggctggtt ccgaacgca gtaaaaaga ccctaaatg 
      tgggtgata gactccgag aatccaaca atattaaca 
      cctctttgt tggtcctgg ggcggtgca tttctgcag 
      tgagaaacg cacaaaagg cgcatgata ataaattgg 
      ttccgttta gagagtcaa aattgaccc aattgttcc 
      aaaaggcgt tatcaggag gcgaagaca ttgcgatga 
      ttgttacta taaatccct cacgcgaaa tgttcgacg 
      aatctcaac acgtgctca acttttccg ccgttgcaa 
      gcgaaatca gcatcgaaa ctgccacca aacagaaag 
      aaacagttc gagatgttt taaaaaata aaacatgta 
      cggaggaag actttgagt acacaagtg aagaactgc 
      aaaacatga ttttgcttc tacgagatg aactcgaaa 
      tccaacgaa acgagtacg aaaccaact tgaaaatcg 
      aaagagagt atttaagga agggctacg aggaaatct 
      accaagacc acgagaatt atgtgcaag aaaggatta 
      aatttgcag aggagcggg tgagaaggc aatacgaag 
      acgaaatcg aggcttata aagataaaa tcaaagagt 
      tggaaaata gatctgaag ttattgaac tgagctcgg 
      atgaagaat cggaagcta aggaaacta gaaggagag 
      ttactaacc ttaggagga ctttagatg aagggtttg 
      gtcgatatc gggcagctg aggaaaaat gcagacaac 
      tcgaaattg aaaatattc aattaaagg agcgaattt 
      ttgaattgg aaaatgagc tagaggcgt tcagacaac 
      atcacgcgt cggtgatca gtgaagaaa ttgaaggtt 
      ccgaaacgt atggtgaaa atggtgtgg cttttaact 
      tatgggatg attaagtaa tcgttgttt tgttattat 
      ttgttttta atttgtttt gccgtttta atttttacc 
      atgagataa atacaatat tttttcagt aaaagttaa 
      ttttgattt aacaccctt ttccttgaa aaattttca 
      tattttatt gaatcacta ctaaggccc tcaattttt 
      ataagttgg aaaggcatt tacataaca atatttcaa 
      taaaaatag cgaattgag
    • >TC013546 RB
      ggtgtgttt gagtttgag tgtaaaagt gattgtttt 
      gtgttgtct ctaccaaag ttattaact tattaagtt 
      tttaaacaa gtaaaaatg tttgaatac gacagtttg 
      gtaatgaaa aatgcttcg ttcttgaga gctcgagat 
      cccagtatt ttaacatac aatcggtcc ttaatacct 
      ggcgcaagg acaaatcta gataaggac tttgctgaa 
      gaaacttat caacaaaaa aatcagccc gaaaccatc 
      cgtgtttat ctccgcata aaaaacggc tatgacttg 
      aaagacttg tacgaaatt gagggaaat accctcgtt 
      tcgaaggtg cccccaggt tcgaatttc ctgcgcaac 
      atcaaagaa ggtgactct ttgaagaaa atccacgct 
      tttacaaaa atcttcgag cctcaaaca acccaaaga 
      gatattttc aatgacgtc gttaaacca aaaattttt 
      gatttcata aatggacag aattccact cttttaact 
      tatggcgct tcaggatct ggaaaaaca ttcactgtc 
      attgggact gatgaagaa ccaggaatc gttcctcgt 
      tctttggaa tatctcttc cgtactttg cctcaactc 
      atgtcagat cctgctgcc aagccatca cctgcagga 
      aatgtgaca ctattgagt gattccgac tgcaagaaa 
      gaaaaattg ctgtgtaga aatctcctg aattcgtct 
      tcagcggtg ggggatcga atgcagcac gtcaggatt 
      tacaaggca atgcaacaa agactgagc agcgaaccg 
      gtcgctttt ctggaagac tgcaatgac ttatctatc 
      ggcgtgtgg atctctttt gccgaaata tacaacgag 
      caaatttac gacctgctt cgcgcaaac ccttcgaaa 
      aaagaacaa cgaccaaga ctgcgctta ggcatgtct 
      aaaggtcaa gtctacatt aaaaacctg acatgcgtg 
      aatgtaaga agtggagag gaagcatat cagatcctc 
      cagtacggg ctaaacaat ctcaattat gcaccaacc 
      cgagtaaac gaacactca agtcgctcc cattcgatt 
      tttacgata aaattggta caagtttca aagtcagat 
      ggtggctgc ttcgttagc agctttaat ttttgtgat 
      ttggctggt tccgaacgc agtaaaaag accctaaat 
      gtgggtgat agactccga gaatccaac aatattaac 
      acctctttg ttggtcctg gggcggtgc atttctgca 
      gtgagaaac gcacaaaag gcgcatgat aataaattg 
      gttccgttt agagagtca aaattgacc caattgttc 
      caaaaggcg ttatcagga ggcgaagac attgcgatg 
      attgttact ataaatccc tcacgcgaa atgttcgac 
      gaatctcaa cacgtgctc aacttttcc gccgttgca 
      agcgaaatc agcatcgaa actgccacc aaacagaaa 
      gaaacagtt cgagatgtt ttaaaaaat aaaacatgt 
      acggaggaa gactttgag tacacaagt gaagaactg 
      caaaacatg attttgctt ctacgagat gaactcgaa 
      atccaacga aacgagtac gaaaccaac ttgaaaatc 
      gaaagagag tatttaagg aagggctac gaggaaatc 
      taccaagac cacgagaat tatgtgcaa gaaaggatt 
      aaatttgca gaggagcgg gtgagaagg caatacgaa 
      gacgaaatc gaggcttat aaagataaa atcaaagag 
      ttggaaaat agatctgaa gttattgaa ctgagctcg 
      gatgaagaa tcggaagct aaggaaact agaaggaga 
      gttactaac cttaggagg actttagat gaagggttt 
      ggtcgatat cgggcagct gaggaaaaa tgcagacaa 
      ctcgaaatt gaaaatatt caattaaag gagcgaatt 
      tttgaattg gaaaatgag ctagaggcg ttcagacaa 
      catcacgcg tcggtgatc agtgaagaa attgaaggt 
      tccgaaacg tatggtgaa aatggtgtg gcttttaac 
      ttatgggat gattaagta atcgttgtt ttgttatta 
      tttgttttt aatttgttt tgccgtttt aatttttac 
      catgagata aatacaata ttttttcag taaaagtta 
      attttgatt taacaccct tttccttga aaaattttc 
      atattttat tgaatcact actaaggcc ctcaatttt 
      tataagttg gaaaggcat ttacataac aatatttca 
      ataaaaata gcgaattga g
    • >TC013546 RC
      caaatttga aaagtttta acggtgtgt ttgagtttg 
      agtgtaaaa gtgattgtt ttgtgttgt ctctaccaa 
      agttattaa cttattaag tttttaaac aagtaaaaa 
      tgtttgaat acgacagtt tggtaatga aaaatgctt 
      cgttcttga gagctcgag atcccagta ttttaacat 
      acaatcggt ccttaatac ctggcgcaa ggacaaatc 
      tagataagg actttgctg aagaaactt atcaacaaa 
      aaaatcagg taaaaacac tcccttggc gttgcagtg 
      atgattttc gttcaattt ttgtgaaat cgtgagaga 
      tgtgtgacg ttaacgaat ctgcttcca cttctttca 
      tcagtttaa gttcaagtg aagaaagct tgaatgacg 
      aaaattgtg atatttgcg ataatgttg agttttttt 
      acacaccac atttcagta actttcgat tgactttcc 
      agcccgaaa ccatccgtg tttatctcc gcataaaaa 
      acggctatg acttgaaag acttgtacg aaattgagg 
      gaaataccc tcgtttcga aggtgcccc caggttcga 
      atttcctgc gcaacatca aagaaggtg actctttga 
      agaaaatcc acgctttta caaaaatct tcgagcctc 
      aaacaaccc aaagagata ttttcaatg acgtcgtta 
      aaccaaaaa tttttgatt tcataaatg gacagaatt 
      ccactcttt taacttatg gcgcttcag gatctggaa 
      aaacattca ctgtcattg ggactgatg aagaaccag 
      gaatcgttc ctcgttctt tggaatatc tcttccgta 
      ctttgcctc aactcatgt cagatcctg ctgccaagc 
      catcacctg caggaaatg tgacactat tgagtgatt 
      ccgactgca agaaagaaa aattgctgt gtagaaatc 
      tcctgaatt cgtcttcag cggtggggg atcgaatgc 
      agcacgtca ggatttaca aggcaatgc aacaaagac 
      tgagcagcg aaccggtcg cttttctgg aagactgca 
      atgacttat ctatcggcg tgtggatct cttttgccg 
      aaatataca acgagcaaa tttacgacc tgcttcgcg 
      caaaccctt cgaaaaaag aacaacgac caagactgc 
      gcttaggca tgtctaaag gtcaagtct acattaaaa 
      acctgacat gcgtgaatg taagaagtg gagaggaag 
      catatcaga tcctccagt acgggctaa acaatctca 
      attatgcac caacccgag taaacgaac actcaagtc 
      gctcccatt cgattttta cgataaaat tggtacaag 
      tttcaaagt cagatggtg gctgcttcg ttagcagct 
      ttaattttt gtgatttgg ctggttccg aacgcagta 
      aaaagaccc taaatgtgg gtgatagac tccgagaat 
      ccaacaata ttaacacct ctttgttgg tcctggggc 
      ggtgcattt ctgcagtga gaaacgcac aaaaggcgc 
      atgataata aattggttc cgtttagag agtcaaaat 
      tgacccaat tgttccaaa aggcgttat caggaggcg 
      aagacattg cgatgattg ttactataa atccctcac 
      gcgaaatgt tcgacgaat ctcaacacg tgctcaact 
      tttccgccg ttgcaagcg aaatcagca tcgaaactg 
      ccaccaaac agaaagaaa cagttcgag atgttttaa 
      aaaataaaa catgtacgg aggaagact ttgagtaca 
      caagtgaag aactgcaaa acatgattt tgcttctac 
      gagatgaac tcgaaatcc aacgaaacg agtacgaaa 
      ccaacttga aaatcgaaa gagagtatt taaggaagg 
      gctacgagg aaatctacc aagaccacg agaattatg 
      tgcaagaaa ggattaaat ttgcagagg agcgggtga 
      gaaggcaat acgaagacg aaatcgagg cttataaag 
      ataaaatca aagagttgg aaaatagat ctgaagtta 
      ttgaactga gctcggatg aagaatcgg aagctaagg 
      aaactagaa ggagagtta ctaacctta ggaggactt 
      tagatgaag ggtttggtc gatatcggg cagctgagg 
      aaaaatgca gacaactcg aaattgaaa atattcaat 
      taaaggagc gaatttttg aattggaaa atgagctag 
      aggcgttca gacaacatc acgcgtcgg tgatcagtg 
      aagaaattg aaggttccg aaacgtatg gtgaaaatg 
      gtgtggctt ttaacttat gggatgatt aagtaatcg 
      ttgttttgt tattatttg tttttaatt tgttttgcc 
      gttttaatt tttaccatg agataaata caatatttt 
      ttcagtaaa agttaattt tgatttaac acccttttc 
      cttgaaaaa ttttcatat tttattgaa tcactacta 
      aggccctca atttttata agttggaaa ggcatttac 
      ataacaata tttcaataa aaatagcga attgag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY