Status
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Gene information
General information
TC012533 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11048995..11056149
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012533
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05262

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012533 RA
      MTLRIIWFF ILIDVIIDA RKNCGKNDI HFEKILGLK 
PSDRNVPLL LYQPASNTT APITTQCLE KCQSNPECQ 
SFVVYFNVS TCYWFRAAV TGAQELELD ADTAWFTKV 
CLKAPKCEK LWVFERVPG ATLVGSDTK ALPSLTRLE 
CQQSCLNER QFDCRSAKF KINSNYGPG DEVRGTCVL 
SEVDRHVMP SAYRASTYD DEYFENQCS KPDEDEDGF 
CTYEEYDNA TLGHSDLFF QNQTKESCR SLCEETQIF 
NCRGYSVVK RNRSFDCYL HSEDSKVHG PRLVQDNFK 
GKYFEKAPC LNISITCSE TYMTVEYNP SMNFMGRMY 
MEGYSENLE CFAKGHGSE IVVLKLPII SEQCGIIKA 
IAPDNRTLL AGTMILQYN PLVQTQGDR IIRVGCIYG 
NDSKVLLGT GITITTAPS HVSPLINST GFAASPVIE 
MLVLDAETQ REVSSTQIG QELELIIEL KQADGSLDI 
WAGHLVAMT EHNDESIIL LDDRGCPTN INIFPALTK 
VATNDSIKL IAKFQAFKF SSSPIVRFS VIVQFCPES 
CPPVDCGNN VQSFGRRKR EIQARAVYT IEGTKVTNV 
NRTEFEKDR AIMKMPLEF IMIVRNPGA NSERLVLGD 
GKILVAGYD FISNEVCLD YSLFIGLII TWILIQIIF 
VTCCIILVR RYKKYYEHE STTQSLEQL NKNFGIGFS 
NLDSRRVHW ADNGDYT
    CDS sequence
    • >TC012533 RA
      atgacgtta cggattatt tggtttttt attttaatt 
gatgttatt atcgatgcc cgaaaaaac tgcggaaaa 
aacgacatc cacttcgag aaaatcctc ggcttgaaa 
ccctccgac cgcaacgtc ccgctcctc ctgtaccaa 
cccgcctcc aacaccacc gcccccatc accacccag 
tgcctcgag aaatgccaa agcaacccc gaatgccaa 
agcttcgtc gtctacttc aacgtcagc acctgctac 
tggttcaga gccgccgtc acgggggcc caggagctg 
gagctggac gccgacacc gcctggttc accaaagtg 
tgcctcaag gcccccaag tgcgaaaaa ctgtgggtc 
tttgagcgc gtccccggc gccactctg gtcggaagc 
gacaccaaa gcactgcca tccctcacc cgcctcgag 
tgccaacaa agctgcctc aacgagagg cagttcgac 
tgcagatcg gccaagttc aagattaac tcgaattac 
ggccccggt gacgaagtc agagggacg tgcgttttg 
agcgaggtt gacaggcat gtcatgccc tcggcatat 
cgggcatca acttacgac gatgagtac tttgagaat 
cagtgctcg aagcccgat gaagacgag gacgggttt 
tgcacgtac gaggagtac gataatgcg acgctgggt 
cacagcgac ttgtttttc caaaatcag accaaagag 
agctgtcgc agtttgtgc gaggagacg cagattttt 
aattgtagg gggtatagt gtagtgaag aggaaccgc 
tcctttgat tgttatttg cacagtgag gatagtaag 
gtgcacggg ccgaggctg gtgcaggat aatttcaag 
gggaaatat ttcgagaaa gcgccatgt ctcaacata 
tcaatcact tgttccgaa acgtacatg acggtcgaa 
tacaacccc agcatgaat ttcatgggg cgcatgtac 
atggagggc tactccgaa aacctcgaa tgtttcgca 
aaaggacac ggaagcgaa atcgtcgtc ctaaaacta 
ccgataatt tccgaacag tgtggaatc atcaaagct 
atagccccc gacaatcgc actttacta gctgggacc 
atgattctg caatacaac ccactggta caaacccaa 
ggcgaccgg attatacga gttgggtgt atttacggg 
aacgatagt aaggtgtta ttggggacg gggatcaca 
attaccacg gctcctagt cacgtcagt cctttgatt 
aattcaacg ggatttgcg gcgtctccg gtcatcgaa 
atgctagtt ttggacgcg gagacccag cgggaagtc 
agcagcaca caaataggc caggagttg gaactaatc 
atcgagctt aagcaagcc gatggttcg ctggatatt 
tgggcaggc catttagtc gcaatgact gaacataat 
gacgaatca attatattg ttagacgat cgaggatgt 
ccgactaac atcaatatt ttcccggcg ttgactaaa 
gtcgcaaca aacgattcg attaaacta attgcgaaa 
ttccaggct tttaaattt tcctcctcg cccatcgtc 
aggttcagc gtgattgta cagttctgc cccgaaagt 
tgtcccccg gttgattgc ggcaacaat gtccagtcg 
ttcggccga agaaagcgc gaaattcaa gcgcgggct 
gtgtacaca atcgagggc acgaaagtc acaaatgtg 
aaccgaaca gaattcgaa aaagaccga gcgattatg 
aaaatgccg ctcgagttc attatgatc gttcgcaac 
cctggtgcc aactccgag cgactggtt ttaggagat 
ggaaaaatt ctagtcgcc ggatatgat tttatctca 
aacgaggtt tgtttggac tactccttg ttcataggc 
ctcataata acgtggatc ttgatccaa atcatcttc 
gtaacgtgt tgtatcatc ctagtgcga cgatacaaa 
aaatactac gagcacgaa agcacaacc cagtcgttg 
gaacagttg aataaaaac ttcggaatc ggcttcagc 
aacctggac agccgacga gtgcattgg gccgataac 
ggcgactat acatga
    mRNA sequence
    • >TC012533 RA
      cgataaacg atggtcaat ttgcattcg actgtttcg 
ggtgatcgt tcatgtaat tgcgtgcgg ttcacttgc 
accacttgt actttattt acctatgtg taaagttca 
gtttttgtt atgacgtta cggattatt tggtttttt 
attttaatt gatgttatt atcgatgcc cgaaaaaac 
tgcggaaaa aacgacatc cacttcgag aaaatcctc 
ggcttgaaa ccctccgac cgcaacgtc ccgctcctc 
ctgtaccaa cccgcctcc aacaccacc gcccccatc 
accacccag tgcctcgag aaatgccaa agcaacccc 
gaatgccaa agcttcgtc gtctacttc aacgtcagc 
acctgctac tggttcaga gccgccgtc acgggggcc 
caggagctg gagctggac gccgacacc gcctggttc 
accaaagtg tgcctcaag gcccccaag tgcgaaaaa 
ctgtgggtc tttgagcgc gtccccggc gccactctg 
gtcggaagc gacaccaaa gcactgcca tccctcacc 
cgcctcgag tgccaacaa agctgcctc aacgagagg 
cagttcgac tgcagatcg gccaagttc aagattaac 
tcgaattac ggccccggt gacgaagtc agagggacg 
tgcgttttg agcgaggtt gacaggcat gtcatgccc 
tcggcatat cgggcatca acttacgac gatgagtac 
tttgagaat cagtgctcg aagcccgat gaagacgag 
gacgggttt tgcacgtac gaggagtac gataatgcg 
acgctgggt cacagcgac ttgtttttc caaaatcag 
accaaagag agctgtcgc agtttgtgc gaggagacg 
cagattttt aattgtagg gggtatagt gtagtgaag 
aggaaccgc tcctttgat tgttatttg cacagtgag 
gatagtaag gtgcacggg ccgaggctg gtgcaggat 
aatttcaag gggaaatat ttcgagaaa gcgccatgt 
ctcaacata tcaatcact tgttccgaa acgtacatg 
acggtcgaa tacaacccc agcatgaat ttcatgggg 
cgcatgtac atggagggc tactccgaa aacctcgaa 
tgtttcgca aaaggacac ggaagcgaa atcgtcgtc 
ctaaaacta ccgataatt tccgaacag tgtggaatc 
atcaaagct atagccccc gacaatcgc actttacta 
gctgggacc atgattctg caatacaac ccactggta 
caaacccaa ggcgaccgg attatacga gttgggtgt 
atttacggg aacgatagt aaggtgtta ttggggacg 
gggatcaca attaccacg gctcctagt cacgtcagt 
cctttgatt aattcaacg ggatttgcg gcgtctccg 
gtcatcgaa atgctagtt ttggacgcg gagacccag 
cgggaagtc agcagcaca caaataggc caggagttg 
gaactaatc atcgagctt aagcaagcc gatggttcg 
ctggatatt tgggcaggc catttagtc gcaatgact 
gaacataat gacgaatca attatattg ttagacgat 
cgaggatgt ccgactaac atcaatatt ttcccggcg 
ttgactaaa gtcgcaaca aacgattcg attaaacta 
attgcgaaa ttccaggct tttaaattt tcctcctcg 
cccatcgtc aggttcagc gtgattgta cagttctgc 
cccgaaagt tgtcccccg gttgattgc ggcaacaat 
gtccagtcg ttcggccga agaaagcgc gaaattcaa 
gcgcgggct gtgtacaca atcgagggc acgaaagtc 
acaaatgtg aaccgaaca gaattcgaa aaagaccga 
gcgattatg aaaatgccg ctcgagttc attatgatc 
gttcgcaac cctggtgcc aactccgag cgactggtt 
ttaggagat ggaaaaatt ctagtcgcc ggatatgat 
tttatctca aacgaggtt tgtttggac tactccttg 
ttcataggc ctcataata acgtggatc ttgatccaa 
atcatcttc gtaacgtgt tgtatcatc ctagtgcga 
cgatacaaa aaatactac gagcacgaa agcacaacc 
cagtcgttg gaacagttg aataaaaac ttcggaatc 
ggcttcagc aacctggac agccgacga gtgcattgg 
gccgataac ggcgactat acatgattt tttattttg 
ttactaact tattacaat aaaaattaa gtgatatcg 
gacgtgttt tcactcttc cttatctct ttggccaag 
tcgatttgt ttctggagg acgtagagg ttctcctgt 
ttgcgcctt tgccgcctc tccatgtct gtcagcact 
ccttggtgt aactgctgc ctgaaat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY