Status
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Gene information
General information
TC011982 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9358356..9363512
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011982
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01912

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011982 RA
      MSSSSNNNT KIKSKQFEK VFKIRNSPL TDRFENSED 
IKTIYNLFA IILISLLTN TVIHDYSKT GQLHFGLRL 
IVRGFNKFG WVVVIWAIF NILAFVLYS VFKFWAKIY 
LLLQPKTQK FWNRIWITI LTLYYILSL YSVQKVVSL 
MDFAPGSAA VLFLEEVRL LMKQHAFIR TNAPKISEY 
KPHSDTKLT LPTFKKFIY FLFAPTFVY RDEYPRTRT 
IRWRFVFYN LGEVLSIIW LLSLVVERF LVPPFEDIC 
IRKYTLSEI LIPVVANSF PGILFMLCG FYSVLHSFQ 
NAFAEMLTF GDRLFYKEW WTSRSLSEY YRTWNVVVH 
DWLFIYIYK DVYEMVIPK NKMAGKLAV FLISALMHE 
WILTNVLKL FLPIQFIQF FIAGAVLAL FNILDLSVG 
NLLVWYLMV FGSGMQVNL YTLEFFART NCPLDTNAT 
IDFFKPRFI TCGCVE
    CDS sequence
    • >TC011982 RA
      atgtcttcg agttcgaat aacaacacg aaaattaaa 
agtaaacag tttgaaaag gtctttaaa atacgcaat 
tctccattg acggaccgc tttgaaaac agtgaagat 
atcaaaaca atttataat ttgttcgct ataattctt 
atcagcttg ttgaccaac actgtaata catgattac 
tctaagacc ggccagtta catttcggg ttgaggctc 
attgtgaga ggatttaat aaatttggc tgggttgtg 
gtcatttgg gctattttc aatattttg gccttcgtg 
ctatacagc gtgttcaag ttctgggcc aaaatttat 
ctactttta caaccaaaa acccaaaaa ttctggaac 
cgaatatgg atcacaatc ctgacttta tactacatc 
ctttcgctc tactccgtg caaaaagtg gtcagttta 
atggatttt gcgcccgga tctgctgcc gttttattc 
ctcgaagaa gtccgactt ttaatgaaa caacatgct 
ttcatccgt acaaacgcc cccaaaatc agcgaatac 
aagccccac agcgacact aaactaacc ctccctacg 
ttcaaaaaa ttcatttac ttccttttc gcccctaca 
tttgtctac agggatgaa tacccccgg acccgcaca 
atacgttgg cgtttcgtt ttttacaat ttaggggaa 
gttttgagc ataatttgg ctgttaagc ctcgttgta 
gagcgattc cttgtaccc ccattcgag gacatttgc 
atcaggaaa tacacttta agcgagatt ctaattcca 
gtggtggca aactcgttc cccggaatt ctattcatg 
ttgtgtggc ttttactcg gttttgcac tcgtttcaa 
aatgctttc gctgaaatg ctcacattc ggggaccga 
ttattttac aaggagtgg tggacctca cgttcgctt 
tcggaatac taccggaca tggaacgta gtagtgcac 
gattggtta tttatttac atctacaaa gacgtttac 
gaaatggtg attcccaag aacaaaatg gcgggaaaa 
ttggccgtt ttcctaatt tcagcactg atgcatgaa 
tggattttg acaaacgtt ttgaaatta ttcctccca 
atccaattc atacaattt ttcatcgcg ggggccgtc 
ctagcccta ttcaatatt ctagactta tcagtcggc 
aatttatta gtctggtat ttgatggtt tttgggtcg 
ggaatgcag gttaatttg tacacactt gaatttttc 
gcacgcaca aactgtcct ttggacaca aacgcgacg 
attgatttc ttcaaacct agatttatc acgtgtggt 
tgtgtggag tag
    mRNA sequence
    • >TC011982 RA
      taacgttat tgttatcac ttatcagtc gtctactgg 
ccgccacta gtttatgaa tctcaataa aatccgaga 
taatttttt gtgattagt tccgtgtgt tcacatgtg 
ccatgtctt cgagttcga ataacaaca cgaaaatta 
aaagtaaac agtttgaaa aggtcttta aaatacgca 
attctccat tgacggacc gctttgaaa acagtgaag 
atatcaaaa caatttata atttgttcg ctataattc 
ttatcagct tgttgacca acactgtaa tacatgatt 
actctaaga ccggccagt tacatttcg ggttgaggc 
tcattgtga gaggattta ataaatttg gctgggttg 
tggtcattt gggctattt tcaatattt tggccttcg 
tgctataca gcgtgttca agttctggg ccaaaattt 
atctacttt tacaaccaa aaacccaaa aattctgga 
accgaatat ggatcacaa tcctgactt tatactaca 
tcctttcgc tctactccg tgcaaaaag tggtcagtt 
taatggatt ttgcgcccg gatctgctg ccgttttat 
tcctcgaag aagtccgac ttttaatga aacaacatg 
ctttcatcc gtacaaacg cccccaaaa tcagcgaat 
acaagcccc acagcgaca ctaaactaa ccctcccta 
cgttcaaaa aattcattt acttccttt tcgccccta 
catttgtct acagggatg aataccccc ggacccgca 
caatacgtt ggcgtttcg ttttttaca atttagggg 
aagttttga gcataattt ggctgttaa gcctcgttg 
tagagcgat tccttgtac ccccattcg aggacattt 
gcatcagga aatacactt taagcgaga ttctaattc 
cagtggtgg caaactcgt tccccggaa ttctattca 
tgttgtgtg gcttttact cggttttgc actcgtttc 
aaaatgctt tcgctgaaa tgctcacat tcggggacc 
gattatttt acaaggagt ggtggacct cacgttcgc 
tttcggaat actaccgga catggaacg tagtagtgc 
acgattggt tatttattt acatctaca aagacgttt 
acgaaatgg tgattccca agaacaaaa tggcgggaa 
aattggccg ttttcctaa tttcagcac tgatgcatg 
aatggattt tgacaaacg ttttgaaat tattcctcc 
caatccaat tcatacaat ttttcatcg cgggggccg 
tcctagccc tattcaata ttctagact tatcagtcg 
gcaatttat tagtctggt atttgatgg tttttgggt 
cgggaatgc aggttaatt tgtacacac ttgaatttt 
tcgcacgca caaactgtc ctttggaca caaacgcga 
cgattgatt tcttcaaac ctagattta tcacgtgtg 
gttgtgtgg agtagttac aataaacgc ttattttat 
ta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY