Status
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Gene information
General information
TC011870 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12364626..12368045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011870
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011870 RA
      MAYLDQREI ANFLAQELF YQQFIALEG INSGVPTRT 
TPTLTPTTL RSIEQTFIE LQTKPESHE NEAGFVPPL 
VHSIGNNQY ISASDADIY SSKSQNMWQ TSLSQSSSD 
SNTRSTPPL TQKTSLINN TPQTRRNMG GRKPARDLN 
ISPEEEERR RIRRERNKA AAARCRKRR VDHTNALIK 
ETEDLEQKK QALQEEIQE YQRMKDELD YLLESHRSS 
SSNCRLHSQ SPPDVKPFV FGSRVNGDR VKPELDEAS 
LPTSNDSFC IPSPAKRVM LSSTAPISK PCRPNSLNV 
MSTAPKNNS ISDIAGIPI TTPSTGIQF NFDSLMVGG 
TGLTPVSAP LVPSCSTQQ RNIPTSAAD MTSPDACGP 
PKLVSL
    CDS sequence
    • >TC011870 RA
      atggcctat ttagaccaa agagaaatc gctaatttt 
ctcgcgcag gagttattt taccagcag ttcatagct 
ctagaaggc atcaattcg ggagtacca actcgaaca 
acccctact ttaactccg acaactctt cgaagtatc 
gagcaaacc tttatagaa ttgcagacg aagccggaa 
tctcacgaa aacgaagcc ggtttcgtc ccacctcta 
gttcattct ataggaaac aatcagtat atttcagcg 
tcggatgct gatatttac tcatcaaag tcacaaaac 
atgtggcaa acttcactt tctcaatct agttcggac 
tcaaacacc aggtcgacg ccacccctg acccagaaa 
actagtctt attaataat acaccgcaa acgaggcgt 
aatatggga gggaggaaa cccgcaaga gatctcaac 
atcagtccg gaagaagaa gaacgaaga cgcatacgg 
agagaacga aataaagcc gcagcggca cgctgcagg 
aaacgcaga gtcgaccac acaaacgct ttaatcaaa 
gagacggaa gatttggag cagaagaag caagcgctc 
caggaggag atccaggag taccaacgc atgaaagac 
gaattggac tacctcctg gaatcgcac cgttcctcc 
tctagcaat tgccgcctg cactcgcag agcccccct 
gacgtcaag ccgttcgtc ttcggaagt cgagtgaac 
ggcgaccgg gtcaagccg gagctggac gaggcgtcg 
ctcccgacg tcaaacgac tccttctgt ataccatcg 
ccagcgaag cgagtgatg ttgagttcg accgctccc 
atatcgaag ccttgcaga ccgaattcg ctgaacgtg 
atgtcgaca gcccccaaa aacaacagc attagcgac 
atagcgggc attccgatc acgacgccc tcgaccggc 
atccagttc aatttcgac tcgctgatg gtggggggc 
acagggctg acgcccgtc tcggccccc ttggtgcct 
tcgtgcagc acccagcag aggaacatc cccacgagt 
gctgccgat atgacgtcg ccggacgcg tgcgggccc 
ccgaaactt gttagttta taa
    mRNA sequence
    • >TC011870 RA
      ggtcaattt ttcgaacga acgattccg atagcaacg 
atattcgaa tatcaataa ttttgattt tgttattac 
atttttaac gattttcgt gtggtacct gcccgaaat 
ggcctattt agaccaaag agaaatcgc taattttct 
cgcgcagga gttatttta ccagcagtt catagctct 
agaaggcat caattcggg agtaccaac tcgaacaac 
ccctacttt aactccgac aactcttcg aagtatcga 
gcaaacctt tatagaatt gcagacgaa gccggaatc 
tcacgaaaa cgaagccgg tttcgtccc acctctagt 
tcattctat aggaaacaa tcagtatat ttcagcgtc 
ggatgctga tatttactc atcaaagtc acaaaacat 
gtggcaaac ttcactttc tcaatctag ttcggactc 
aaacaccag gtcgacgcc acccctgac ccagaaaac 
tagtcttat taataatac accgcaaac gaggcgtaa 
tatgggagg gaggaaacc cgcaagaga tctcaacat 
cagtccgga agaagaaga acgaagacg catacggag 
agaacgaaa taaagccgc agcggcacg ctgcaggaa 
acgcagagt cgaccacac aaacgcttt aatcaaaga 
gacggaaga tttggagca gaagaagca agcgctcca 
ggaggagat ccaggagta ccaacgcat gaaagacga 
attggacta cctcctgga atcgcaccg ttcctcctc 
tagcaattg ccgcctgca ctcgcagag cccccctga 
cgtcaagcc gttcgtctt cggaagtcg agtgaacgg 
cgaccgggt caagccgga gctggacga ggcgtcgct 
cccgacgtc aaacgactc cttctgtat accatcgcc 
agcgaagcg agtgatgtt gagttcgac cgctcccat 
atcgaagcc ttgcagacc gaattcgct gaacgtgat 
gtcgacagc ccccaaaaa caacagcat tagcgacat 
agcgggcat tccgatcac gacgccctc gaccggcat 
ccagttcaa tttcgactc gctgatggt ggggggcac 
agggctgac gcccgtctc ggccccctt ggtgccttc 
gtgcagcac ccagcagag gaacatccc cacgagtgc 
tgccgatat gacgtcgcc ggacgcgtg cgggccccc 
gaaacttgt tagtttata aagaaaagc gctgtagcg 
agatgtgtg ttatgtggg gaaaagttt catgttgtt 
gtattattt tctaattaa actaagagt ttatttatt 
atatttttg gactttttt tcacatacg catgtcgag 
ctacgggct aggttagcc gagagatca atgttggac 
gagctcatc tttagcgaa aaagtgaaa ttttttagg 
gattttatg actgccgcc gcatgtcca acgtgttta 
ccaaccatg tttagtatt cttgaaacc aaaccaaaa 
atatttatt actaattaa tttattagt aatgttcta 
gtgtcattt ttgcattat ttattgtta tgttttttt 
aaatagtca tttgtatat aacgtgtgt actaaacat 
ggaccaaaa atgtcgtca aagtgctca agctatttt 
gctatgcta aatgcagat ttatttttt gctaccaag 
aaatgcctt ttttgtaat ggtaaaaaa atctgtata 
ttttcactt aacgcaaag ttattccct ccatacatt 
tttatatga tagatttaa ggttaatgt tatattttt 
gcaatagga gtgctccta ttccaataa gcgaatttg 
tgttactac tattaagaa tataattaa taatatatt 
gagaatact ttaacaaat aggactatc agcaatact 
gtggttgca atatttgta atcactcgt caattttat 
atagagttt gtcaaattt tgtatttat tttatgcga 
tgccaatta taaaaaata aaatctatc ttttaag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY