Status
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Gene information
General information
TC011652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16016547..16045471
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011652
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head capsule split - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011652 RA
      MELENIVAN TVYLKAREG GSDSNKGKS KKWRKILQF 
PHISQCIDL INKIDVRYE YVVDQQPIG KLLFEQWCE 
VKRHEYHRC VKFLDIAKR YEVETDEQR LELANAIKK 
NSWEFTNSM YFHRYLQWK WLEAQPVTY KTFRMYRVL 
GKGGFGEVC ACQVRATGK MYACKKLEK KRIKKRKGE 
AMVLIEKQI LQKINSRFV VNLAYAYET KDALCLVLT 
IMNGGDLKF HIYNMGGEP GLDIARARF YAAEVVCGL 
DHLHQQGIV YRDCKPENI LLDDAGHVR ISDLGLAVD 
IPEGEFVRG RVGTVGYMA PEVIDNERY TFSPDWFSF 
GCLLYEMIE GQAPFRARK EKVKREEVD RRVKSDQEK 
YSSKFSEDA KSLCQQLLA KSPAQRLGG KAGRYGAAV 
VKQHAFFQS LNWRRLEAG MVEPPFVPD PHAVYAKDV 
LDIEQFSTV KGVNIDEND SSFYSKFNS GCVSIPWQN 
EMIETDCFR QLNVFGANS TRSSDLILQ PVTTTENEG 
CFPFRRKKK QLPRTKPIP INPSLLPQM TES
    CDS sequence
    • >TC011652 RA
      atggaattg gaaaatatc gtcgccaac acagtctat 
ctaaaagcg agagagggt ggttccgat agtaataaa 
ggcaagagc aaaaagtgg aggaagatt ctgcagttt 
ccccatatc tcgcagtgt atagatctt atcaacaaa 
atagatgtt cgctacgag tacgtggtg gaccagcag 
cccatcgga aagctcttg ttcgagcag tggtgtgaa 
gtgaagcgt catgaatac caccgctgt gtcaagttc 
ctcgacatc gccaaacgc tatgaagtc gaaacggac 
gaacaacgg ttggagttg gcaaacgcc atcaaaaag 
aattcatgg gaattcact aattctatg tattttcat 
cggtacctg cagtggaag tggctggaa gcgcaaccg 
gtcacttac aagactttt aggatgtat cgcgtcttg 
ggcaagggc ggattcgga gaggtctgc gcttgtcag 
gtgcgagcg actggtaaa atgtacgcg tgtaaaaag 
ctggaaaag aaacggata aagaaacgc aaaggcgaa 
gcgatggtt ctgatcgag aagcagata ttgcaaaag 
atcaattca cgatttgtt gtgaatctt gcctacgct 
tacgaaacg aaagacgcc ctttgttta gtgttgacg 
ataatgaat gggggcgat ttaaagttt cacatctac 
aacatgggt ggagaaccg ggactggac attgcgagg 
gcgcgattt tacgcagct gaagtcgtc tgtggactt 
gatcatttg catcagcag ggcattgta tatagagac 
tgtaaaccc gaaaacatt cttctcgac gatgcaggt 
cacgtaagg atctcagat ttaggttta gccgtagac 
atccctgaa ggagaattc gttagaggt agagtcggc 
actgtaggc tacatggca cctgaagtt atagataac 
gaacgttac acattcagt cccgattgg ttcagtttc 
ggttgtttg ttgtacgaa atgattgaa ggccaggca 
ccgttccga gcgcgtaaa gaaaaagtt aaacgtgaa 
gaagtggat cgacgagtg aagtccgac caggagaaa 
tactcgtcg aaattcagt gaagatgcc aaatctttg 
tgccaacaa ttactagcc aaaagtccc gcacagaga 
ctggggggc aaagcggga cgatatggg gctgctgta 
gtcaaacaa cacgctttt tttcagagt cttaattgg 
aggagactc gaggcaggg atggtcgaa ccccctttc 
gtaccagat cctcatgcg gtttacgcc aaagacgtg 
ctcgatatc gagcagttt tccaccgtc aaaggcgtg 
aatattgac gagaacgat tcctcgttt tatagcaag 
ttcaacagt ggttgtgtg tccatacca tggcaaaat 
gaaatgata gaaacagac tgctttaga caattaaat 
gtgtttggt gcgaacagt acgagaagc agcgattta 
atacttcaa cccgtcacg acgacagaa aacgaaggt 
tgctttcct ttccgacga aagaagaaa cagctgcct 
aggaccaag cctatcccc ataaacccc tccttgtta 
cctcaaatg acagagagc tga
    mRNA sequence
    • >TC011652 RA
      taagttgca atcaaacat catataact ggtcatgtg 
accaaattc aacgaatcg gatgctcaa actcatgtg 
tgataatta attgggcat taattttta acactgaat 
cgtcaatta atttttaat tagactatt gttaaacca 
gcttgtagt ttgtcttag ttgttttgc tcttccata 
gaaaaaaat ctgcgagat ttttggttt ttcatttga 
agatcgatc gtttggatc cccacccga gcgtcacca 
ccgccgcat cgcctcctc gtgtgaacc tctccgtag 
ttctgtttt cgtcctctt cgttgtggt gtcgctctg 
ttcggtgga tagacgcgt gtcggtagc tgtgtgtac 
ctagtaaag tcggtgaaa tttgttgag aaagtcgtc 
tttgagtgc atcagggtg gcattccaa gggtcgcag 
acacacccc gaagtggcg gcagcatcc ccttgggtg 
cgcatcgag ggtgcaagt gcatcagcc cgaccccca 
accaccccc ataattaat ggaaacggg tcgttcacg 
acccgaccc cccgggtaa aaacaacca ccaaactgg 
aactcatgg aattggaaa atatcgtcg ccaacacag 
tctatctaa aagcgagag agggtggtt ccgatagta 
ataaaggca agagcaaaa agtggagga agattctgc 
agtttcccc atatctcgc agtgtatag atcttatca 
acaaaatag atgttcgct acgagtacg tggtggacc 
agcagccca tcggaaagc tcttgttcg agcagtggt 
gtgaagtga agcgtcatg aataccacc gctgtgtca 
agttcctcg acatcgcca aacgctatg aagtcgaaa 
cggacgaac aacggttgg agttggcaa acgccatca 
aaaagaatt catgggaat tcactaatt ctatgtatt 
ttcatcggt acctgcagt ggaagtggc tggaagcgc 
aaccggtca cttacaaga cttttagga tgtatcgcg 
tcttgggca agggcggat tcggagagg tctgcgctt 
gtcaggtgc gagcgactg gtaaaatgt acgcgtgta 
aaaagctgg aaaagaaac ggataaaga aacgcaaag 
gcgaagcga tggttctga tcgagaagc agatattgc 
aaaagatca attcacgat ttgttgtga atcttgcct 
acgcttacg aaacgaaag acgcccttt gtttagtgt 
tgacgataa tgaatgggg gcgatttaa agtttcaca 
tctacaaca tgggtggag aaccgggac tggacattg 
cgagggcgc gattttacg cagctgaag tcgtctgtg 
gacttgatc atttgcatc agcagggca ttgtatata 
gagactgta aacccgaaa acattcttc tcgacgatg 
caggtcacg taaggatct cagatttag gtttagccg 
tagacatcc ctgaaggag aattcgtta gaggtagag 
tcggcactg taggctaca tggcacctg aagttatag 
ataacgaac gttacacat tcagtcccg attggttca 
gtttcggtt gtttgttgt acgaaatga ttgaaggcc 
aggcaccgt tccgagcgc gtaaagaaa aagttaaac 
gtgaagaag tggatcgac gagtgaagt ccgaccagg 
agaaatact cgtcgaaat tcagtgaag atgccaaat 
ctttgtgcc aacaattac tagccaaaa gtcccgcac 
agagactgg ggggcaaag cgggacgat atggggctg 
ctgtagtca aacaacacg ctttttttc agagtctta 
attggagga gactcgagg cagggatgg tcgaacccc 
ctttcgtac cagatcctc atgcggttt acgccaaag 
acgtgctcg atatcgagc agttttcca ccgtcaaag 
gcgtgaata ttgacgaga acgattcct cgttttata 
gcaagttca acagtggtt gtgtgtcca taccatggc 
aaaatgaaa tgatagaaa cagactgct ttagacaat 
taaatgtgt ttggtgcga acagtacga gaagcagcg 
atttaatac ttcaacccg tcacgacga cagaaaacg 
aaggttgct ttcctttcc gacgaaaga agaaacagc 
tgcctagga ccaagccta tccccataa acccctcct 
tgttacctc aaatgacag agagctgat gcctctaat 
aatttaatt caatgtcaa cacactttc ctccccaac 
gtctcatat tcgtcttcc ctgaaattt acgcacgac 
gattgttac attttttgt atatattta agtgttgaa 
agcatgcac aatgccatt ttggtagat gatatattt 
tttataagt ttatttttt tgcgtgaga ttactttcg 
ggagtcatc tactgatgt ggaaacggt tagttacac 
agcttccgt gatagatag acagttaat taaaacgtc 
tatatatca tgaaggctg tggttagtt gtctcaaaa 
acgtatttg aatgttgtt ttgttttca ttccacaat 
cacgtcacc taatggatt agataattg atgtatttc 
aagaaggaa gaaaaaaga ccttttata ttattttat 
tgtgatatt ttatctgtg tgtttgtgt ctaccagga 
cttgatttc aacggccca ataataaag ttcaaaaca 
gattttaac ttgcccaag aatttgtct acattaagc 
tcaaatatt aggatagaa atccattaa ctttcaatt 
gtatgtata agcagtttg tattgtttt tttatttaa 
tttatacat tttttgagc gactgtttt ttgtaaatt 
cgttggtcc gtcactgtg agatcatta aacagagtg 
aatttaaaa aaaaacgtc caaacgttt gtatgtata 
ctccaggaa acaattcct gactcacgt tgactgtcc 
aaccaaact tttttttaa attatgcct gttttgact 
ttgtattga aatgatttt tgataatta attaaacat 
ctaaatcat aa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY