Status
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Gene information
General information
TC011295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19632792..19641569
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011295
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01837

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_01837

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011295 RA
      MANDCEKPG GAVKRSAQK PETGEMASP EPKRLKVDL 
      SLMSDQNPV SVLNQLRVG LKYNFIEQR GPSHAPLFK 
      VAVEVDGQT YYGVGGSKK LAKCKAAEE ALKSFIQFP 
      NNTAYAVGG NGTNVDFTS DAFEAEKKE GPTKKVATK 
      GPVMLLNEL YPNVVYTCV ENDGSAYAR FKMTVTIGD 
      EKFIGTGSS KKQAKNAAA TTALAKLNL KTSLNGDQK 
      SSVLLEEQE KADYVGRLI QEKFEALMS TDPTHIKRK 
      VLSGIVMTR STSLHDSEI ISVTTGTKC ISGEHISMN 
      GCSLNDMHA EILSRRCLI TYFYDQLEL IANSQPEKS 
      IFTQREDGK GYKLKPGLD FHLYINTAP CGDARIFSP 
      HEESEAVDK HPNRCSRGQ LRTKIESGE GTIPVKASG 
      SLIQTWDGI LQGERLLTM SCSDKICRW NVVGVQGAL 
      LSHFIEPIY LKSIVLGSL MREAHMYRA LCGRIENTI 
      QGLPPPFRL NRPSMYLLT SSETRQPKK APNFAVVWA 
      QGDSRPEIV NTTVGKPEN GFSKLCKVE LMKRFARLV 
      GRISSVTEI EKNLPSLYC DVKDAVASY KAAKTNLYE 
      AFLKAGLGN WISKPMEQD MFELREGND
    CDS sequence
    • >TC011295 RA
      atggccaat gattgtgaa aaacctggg ggcgcagtt 
      aaaaggagt gctcagaag cctgaaaca ggcgagatg 
      gcgtctccg gagcccaag cggctcaaa gtggacttg 
      tctttgatg agcgaccaa aaccccgtt tcagtccta 
      aaccagtta agagtaggt ttaaagtac aattttatc 
      gaacagcgg ggcccttca cacgccccg ctgttcaaa 
      gtcgccgta gaagtggat ggtcagact tactacggc 
      gttggcggt tcgaaaaaa ctcgccaaa tgtaaagcg 
      gccgaagag gccttaaaa tcgttcatc cagtttccg 
      aataacaca gcgtatgct gttggagga aacggcact 
      aatgtcgat tttacgagc gatgccttc gaagccgag 
      aaaaaagaa gggcccacg aaaaaagta gcgactaaa 
      gggccggtt atgctcttg aatgaactt tatccaaat 
      gtggtctat acgtgtgtg gagaatgat ggaagtgcg 
      tatgctagg tttaagatg acggtgacc attggggac 
      gaaaaattt atcgggact ggttccagt aaaaaacaa 
      gcgaaaaat gcggctgca accaccgcc ttagccaag 
      cttaactta aaaacgagt ctaaatgga gatcaaaaa 
      tcaagcgtg ctgttggag gaacaagaa aaagccgat 
      tatgttgga agattaatc caagagaaa ttcgaagcc 
      ctcatgtcc accgacccc acccacatc aaacgcaaa 
      gtcctctcg ggcatcgta atgacccgt tcaacgtcc 
      ctccacgac tctgaaata atttcagtg accaccggc 
      acaaaatgc atctccggc gagcacatc agcatgaac 
      gggtgttcc cttaacgac atgcacgca gaaatcctc 
      tcccgccgt tgtctcatc acctacttc tacgaccaa 
      ctcgagctc attgcaaac tcacagccc gaaaaatca 
      atcttcacc caacgtgaa gacggcaaa ggctacaaa 
      ctcaaaccg ggtctagac ttccacctg tacatcaac 
      acagccccc tgcggcgac gcgcgcatt ttcagcccc 
      catgaggag agcgaggcg gtggacaag caccccaat 
      cgctgctca agggggcaa ctgcgcacc aaaatcgag 
      tcaggcgag ggcacaatc cccgtcaaa gccagcggg 
      agtttgata caaacctgg gacgggatt ttgcaaggg 
      gagagactc ctaaccatg tcctgctcg gataagatc 
      tgtcgctgg aacgttgtg ggggtacag ggggccctc 
      ctgtcccat ttcatcgaa ccgatttac ctcaaatcc 
      atcgttttg gggagcttg atgcgcgag gcgcacatg 
      tacagagcc ctctgtggc cggattgag aacacgatt 
      caaggccta ccgccccca tttcggcta aaccgcccc 
      tcgatgtat ttactgact tcgagtgag acacggcag 
      cctaaaaaa gcgccaaac tttgccgtg gtgtgggca 
      caaggtgat tctagaccg gaaattgtt aatacaacg 
      gtgggaaaa cccgaaaat gggttttcg aaattgtgc 
      aaagtcgag ttgatgaag aggtttgcg cgattagtt 
      gggagaatc tcaagtgtg actgagatt gagaaaaat 
      ttaccgagc ctttattgt gacgttaaa gacgccgta 
      gcttcgtat aaggccgcc aaaaccaat ttgtacgaa 
      gcgttcctc aaagcgggc ttgggtaat tggatttcc 
      aaacccatg gaacaggat atgttcgaa ttgagggag 
      ggaaacgac tag
    mRNA sequence
    • >TC011295 RA
      tgaggttat tttaaccgt agataatta tttgttttg 
      caacaaaac caaattttt tgtgttaac aaatgtccc 
      aacagttgt tattttacg ttaaagcca aactttaac 
      tttttgtga gcatggcca atgattgtg aaaaacctg 
      ggggcgcag ttaaaagga gtgctcaga agcctgaaa 
      caggcgaga tggcgtctc cggagccca agcggctca 
      aagtggact tgtctttga tgagcgacc aaaaccccg 
      tttcagtcc taaaccagt taagagtag gtttaaagt 
      acaatttta tcgaacagc ggggccctt cacacgccc 
      cgctgttca aagtcgccg tagaagtgg atggtcaga 
      cttactacg gcgttggcg gttcgaaaa aactcgcca 
      aatgtaaag cggccgaag aggccttaa aatcgttca 
      tccagtttc cgaataaca cagcgtatg ctgttggag 
      gaaacggca ctaatgtcg attttacga gcgatgcct 
      tcgaagccg agaaaaaag aagggccca cgaaaaaag 
      tagcgacta aagggccgg ttatgctct tgaatgaac 
      tttatccaa atgtggtct atacgtgtg tggagaatg 
      atggaagtg cgtatgcta ggtttaaga tgacggtga 
      ccattgggg acgaaaaat ttatcggga ctggttcca 
      gtaaaaaac aagcgaaaa atgcggctg caaccaccg 
      ccttagcca agcttaact taaaaacga gtctaaatg 
      gagatcaaa aatcaagcg tgctgttgg aggaacaag 
      aaaaagccg attatgttg gaagattaa tccaagaga 
      aattcgaag ccctcatgt ccaccgacc ccacccaca 
      tcaaacgca aagtcctct cgggcatcg taatgaccc 
      gttcaacgt ccctccacg actctgaaa taatttcag 
      tgaccaccg gcacaaaat gcatctccg gcgagcaca 
      tcagcatga acgggtgtt cccttaacg acatgcacg 
      cagaaatcc tctcccgcc gttgtctca tcacctact 
      tctacgacc aactcgagc tcattgcaa actcacagc 
      ccgaaaaat caatcttca cccaacgtg aagacggca 
      aaggctaca aactcaaac cgggtctag acttccacc 
      tgtacatca acacagccc cctgcggcg acgcgcgca 
      ttttcagcc cccatgagg agagcgagg cggtggaca 
      agcacccca atcgctgct caagggggc aactgcgca 
      ccaaaatcg agtcaggcg agggcacaa tccccgtca 
      aagccagcg ggagtttga tacaaacct gggacggga 
      ttttgcaag gggagagac tcctaacca tgtcctgct 
      cggataaga tctgtcgct ggaacgttg tgggggtac 
      agggggccc tcctgtccc atttcatcg aaccgattt 
      acctcaaat ccatcgttt tggggagct tgatgcgcg 
      aggcgcaca tgtacagag ccctctgtg gccggattg 
      agaacacga ttcaaggcc taccgcccc catttcggc 
      taaaccgcc cctcgatgt atttactga cttcgagtg 
      agacacggc agcctaaaa aagcgccaa actttgccg 
      tggtgtggg cacaaggtg attctagac cggaaattg 
      ttaatacaa cggtgggaa aacccgaaa atgggtttt 
      cgaaattgt gcaaagtcg agttgatga agaggtttg 
      cgcgattag ttgggagaa tctcaagtg tgactgaga 
      ttgagaaaa atttaccga gcctttatt gtgacgtta 
      aagacgccg tagcttcgt ataaggccg ccaaaacca 
      atttgtacg aagcgttcc tcaaagcgg gcttgggta 
      attggattt ccaaaccca tggaacagg atatgttcg 
      aattgaggg agggaaacg actaggtca ctaattttc 
      aaattttac ttctgtcat ttttacaga atatatttt 
      aatttttga cttcataag tctatgaat tttattcta 
      ggaattaaa ataatttta acaaaccat a

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY