Status
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Gene information
General information
TC010107 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14857846..14861042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010107
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04762

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010107 RA
      MEFEQFCNF LPKIELHAH LNGSLSATS LQKLGCSNE 
EISEYQKLA ELQATEKTL NECFKLFKV AHNATKNPQ 
AVYLATKYV IEDFYNDNV AYLELRTTP REEENMSRV 
EYIESVVKA IEDCDKKII VKLLLSIDR SNNLKVEEN 
MEVIIKMKE KYPHVIKGV DFSGNPYVG GFNPKLFQK 
ARDSGLFVT LHCAEIKND KEVEEILKF RPDRIGHGT 
FLLSNDHIW KLYLDTNIP LECCLTSNV ACGTTKSYK 
EHHLQEWIK NSLPFTLCT DDKGVFGTT LSKELVLAC 
QYFSLKPTD LWDMTLKTI SYTFASDEE KTFLTTTLQ 
DWKMKNHF
    CDS sequence
    • >TC010107 RA
      atggaattt gaacagttt tgtaacttt ttaccaaaa 
atagaattg cacgcacac ctcaatggt tctttgagt 
gccactagt ttacaaaaa ctgggttgt tccaatgaa 
gaaatatct gaataccaa aaattagca gagttgcaa 
gcaaccgaa aaaacccta aatgaatgt ttcaagttg 
tttaaagtt gcccacaat gccacgaaa aatccgcaa 
gcggtttac ttggccaca aaatatgta attgaggat 
ttttacaac gataatgtg gcgtattta gaattgcga 
acaacaccg agagaagaa gaaaacatg tcaagagtg 
gagtatatt gaatcggtg gttaaagca atcgaagat 
tgtgataaa aaaatcatc gtgaagttg cttttgtca 
attgataga agcaacaat ttgaaagtt gaagaaaac 
atggaagtg attataaaa atgaaagaa aagtatccg 
catgtaata aaaggcgtt gactttagt ggcaaccct 
tacgtcggc ggtttcaac ccgaaatta ttccaaaaa 
gcacgagat tctggctta tttgtcact ctacactgt 
gctgaaata aaaaatgat aaggaagtt gaagaaatt 
cttaaattt agacccgac aggattggg cacggaaca 
tttttgctt tcaaacgac cacatttgg aaattatat 
ctcgacacg aacatacca ttggagtgt tgtttgaca 
tcaaacgtg gcgtgtgga accacaaaa agttacaaa 
gagcatcat ttgcaagag tggattaag aattcgttg 
ccgttcact ttatgtacc gatgataag ggagtattt 
ggcaccact ttatccaaa gagttggtg ttagcctgc 
cagtatttt tctctaaaa ccaactgat ctctgggac 
atgactctg aaaacgatc agttacaca tttgcctca 
gacgaggaa aagacattt ctcaccaca actttacaa 
gactggaaa atgaaaaat cacttctaa
    mRNA sequence
    • >TC010107 RA
      accagcacc agccaaacg acagtgttt tgtttataa 
ccacgtgcg aaatggaat ttgaacagt tttgtaact 
ttttaccaa aaatagaat tgcacgcac acctcaatg 
gttctttga gtgccacta gtttacaaa aactgggtt 
gttccaatg aagaaatat ctgaatacc aaaaattag 
cagagttgc aagcaaccg aaaaaaccc taaatgaat 
gtttcaagt tgtttaaag ttgcccaca atgccacga 
aaaatccgc aagcggttt acttggcca caaaatatg 
taattgagg atttttaca acgataatg tggcgtatt 
tagaattgc gaacaacac cgagagaag aagaaaaca 
tgtcaagag tggagtata ttgaatcgg tggttaaag 
caatcgaag attgtgata aaaaaatca tcgtgaagt 
tgcttttgt caattgata gaagcaaca atttgaaag 
ttgaagaaa acatggaag tgattataa aaatgaaag 
aaaagtatc cgcatgtaa taaaaggcg ttgacttta 
gtggcaacc cttacgtcg gcggtttca acccgaaat 
tattccaaa aagcacgag attctggct tatttgtca 
ctctacact gtgctgaaa taaaaaatg ataaggaag 
ttgaagaaa ttcttaaat ttagacccg acaggattg 
ggcacggaa catttttgc tttcaaacg accacattt 
ggaaattat atctcgaca cgaacatac cattggagt 
gttgtttga catcaaacg tggcgtgtg gaaccacaa 
aaagttaca aagagcatc atttgcaag agtggatta 
agaattcgt tgccgttca ctttatgta ccgatgata 
agggagtat ttggcacca ctttatcca aagagttgg 
tgttagcct gccagtatt tttctctaa aaccaactg 
atctctggg acatgactc tgaaaacga tcagttaca 
catttgcct cagacgagg aaaagacat ttctcacca 
caactttac aagactgga aaatgaaaa atcacttct 
aacaaaaca gttttatat ttcagatta taaaaaata 
caataaata acttatatt aatactttt atgaaggta 
tggcctttg acgtgtgtt gagcacagc ttcctagat 
caatgaact aacaccata cggattatt aatctttta 
tcaatcaag gaagctgtc aaaaacgtc aaaaattcc 
cacctatga actaatacg tcgttttga gtagcattc 
ctataaatg aatacctac caaaagtaa aacaaaaac 
tggtttatg tgatgttag tgtgagtat ttataaagc 
gaaaaggaa acaatgtag aataaaaat tgcacttaa 
atagggcac atcacaatt ttgttacac aaatttcac 
ctaaaggct aagctgcca tcgacaaac atcaaaata 
ctacagcta tatacccgt tctcaaatg attggtttt 
tcgtattat tctaatttc catttaatt tcgttttga 
tcataacct ccttaactc gtgtcaagg aggctgtgg 
cttggatgc aggagaaag gaagaggtc aacggccag 
tatttgatt ctaccctgt tttcctcta ctattccac 
aatgaattt ttagatgag catgagaca agtgcaaca 
gctataaat ttcgtaaac ataacggtt atagaattt 
tcagcgctg ggaacagga agatactta acgattata 
attgttagc ctcattgtg gcacaatga aataaaata 
taaaaatat caacaacaa acaaatcct taactacgt 
taaatgcaa tgattggtc ttggctgtg tcctatgat 
gttcgatta tcgaatcaa gacatttat acctaaaat 
attatagta tatacatgc agtacaatt tcaatgcga 
aagacttgg gttactagt cacgaatga taaaataca 
cagaccaca acaaaatat atctttttt atttataca 
acatgtaaa cggattttt tccaacatt tatgagtta 
tgagctgct tttgtggtt cacaacttt aggcgcttt 
gctcaaaat tcaattgga ttggaacgt gcccaacaa 
tggcagtaa ttaatcata aactagtga ttaagcaat 
tagtaccta ttattaaac gataatccg atctatttt 
tagagttaa gtaggtggt tggagcgtc aatataacc 
agattcaca aacatttat ctgtgaaat aaaaagtac 
ttgtagact ttaaataaa aaaacaagt tttctggag 
taaatattt gtaaatcca ccttatact gatcaaaat 
gtaaggttt attgtcttt tataaatgt tcattttta 
aatactgaa tcttgatgt gttatttca gttaattac 
gtgtatgaa acagattca ggtaacttt aaaatactt 
ttgtttttc ttcgattag agtcatgcg tttgctgaa 
taaaacaaa tcgttgcaa ttctacatt cacgcgttt 
ataaataaa tatttttga ataattttt tatgctgta 
tgtttaata atgttttac agaattgtg cgcgttaaa 
acgccatga atttattca gctttttat gtgtttttc 
ttgcttcgt tgttacagc tcacgattt cttacagtt 
taggaacat caaacaaat tttatttta tttacattt 
ttactaaca aacaataat aattattgc acttatttt 
aaatgtaaa cagaccagt cattctagc ttctccaaa 
atattgtaa accaaagca tgatgaaag acagaaatg 
atagtaaac gtgtatttt attataatt agaaattac 
ctgcaatcc cttcaaata agattcggc tacattttc 
cactctata tgcaaataa tctatcagc ttatttttg 
aactaaaaa catgctcaa acgttgaaa aaaggcaaa 
tttgactta atttgactg ttggcccaa cactgcgat 
tttgagagt ttaaaactc aaaaagtgc ttaaaagct 
cgaataagt aatcattct aactaaatt tggcgaaaa 
aat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY